Gene:
Gene Symbol:
Disc1
Gene Name:
disrupted in schizophrenia 1
Original Clone ID:
FLH270439.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083363
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2556nts
Open reading frame: 1 to 2556
ATGCAGGGCGGGGGTCCCCGGGGCGCTCCGATCCACAGTCCGAGCCACGGCGCAGACAGT
GGGCATGGCTTACCGCCTGCAGTAGCCCCTCAGAGGCGGCGGCTGACACGGAGACCAGGC
TACATGAGAAGCACAGCGGGTTCTGGGATCGGGTTCCTCTCTCCAGCAGTGGGCATGCCA
CACCCGAGCTCAGCAGGGCTGACAGGCCAGCAGTCCCAACACTCACAGTCCAAGGCTGGA
CAGTGCGGACTTGACCCTGGGAGCCACTGCCAAGCCTCACTGGTGGGCAAGCCTTTTCTC
AAGAGCTCCCTTGTCCCTGCTGTGGCCTCTGAGGGCCACCTGCACCCAGCCCAGCGCTCT
ATGAGAAAAAGACCAGTGCACTTTGCGGTTCATTCCAAGAATGACAGCAGACAATCTGAG
AGGCTGACTGGGTCATTTAAGCCTGGGGACAGTGGGTTTTGGCAAGAATTATTATCTTCA
GACAGCTTTAAGTCTCTGGCTCCTAGCCTTGATGCACCCTGGAACAAGGGATCAAGGGGC
CTGAAGACTGTGAAACCTCTGGCATCACCGGCGTTGAATGGCCCCGCTGATATCGCATCC
CTTCCCGGCTTCCAAGACACCTTCACTTCCAGCTTCAGCTTCATCCAACTCTCCCTTGGT
GCTGCTGGAGAACGCGGAGAAGCAGAAGGTTGCCTGCCATCCAGAGAGGCCGAACCTCTG
CATCAGAGGCCCCAAGAGATGGCAGCTGAAGCATCTAGCTCAGACAGGCCCCACGGGGAT
CCTCGGCATCTCTGGACCTTCAGTCTTCACGCTGCTCCAGGCTTGGCGGACTTGGCTCAG
GTGACAAGGAGCAGCAGCAGGCAATCAGAATGTGGCACGGTCTCCTCCTCCTCCTCGGAC
ACTGGCTTCTCTTCCCAGGATGCATCCTCCGCTGGTGGGCGGGGCGACCAGGGCGGCGGC
TGGGCCGATGCCCATGGATGGCATACATTGCTCAGGGAATGGGAGCCCATGCTGCAGGAC
TACCTACTGAGCAACCGCAGGCAGCTGGAGGTCACTTCCTTAATTTTAAAGCTTCAGAAA
TGTCAAGAAAAAGTGGTCGAGGATGGCGATTACGATACTGCAGAGACATTGAGACAGAGG
TTGGAAGAACTGGAACAGGAGAAAGGCCGCCTGTCCTGGGCTCTGCCTTCACAGCAACCT
GCTCTTCGCAGCTTCTTGGGTTACCTGGCAGCACAGATACAGGTGGCCTTGCATGGAGCC
ACCCAAAGGGCCGGCAGCGATGATCCAGAAGCCCCACTTGAAGGACAGCTGAGGACTACC
GCCCAGGATAGCCTGCCTGCATCCATCACCAGGAGGGACTGGCTTATTCGAGAGAAACAG
CGATTGCAGAAGGAAATCGAAGCTCTCCAAGCACGGATGTCTGCGCTGGAGGCAAAGGAA
AAACGGCTGAGCCAAGAGTTGGAGGAGCAGGAGGTGCTGCTCCGGTGGCCGGGCTGTGAC
CTGATGGCACTGGTGGCCCAGATGTCCCCAGGCCAGCTGCAGGAGGTCAGCAAGGCCTTG
GGAGAGACCCTGACCTCTGCCAACCAGGCTCCCTTCCAGGTGGAGCCACCTGAGACCCTC
AGGAGCCTCCGGGAAAGGACAAAATCACTGAACCTGGCTGTCAGAGAACTCACTGCTCAG
GTGTGCTCAGGTGAGAAGCTGTGCAGCTCTCTGAGGAGGAGACTCAGTGACCTCGACACC
AGGCTGCCTGCCTTGCTGGAAGCCAAGATGCTGGCCCTATCAGGAAGCTGCTTCTCCACA
GCCAAGGAGCTCACGGAGGAGATTTGGGCCTTGTCGTCAGAGCGGGAAGGGCTAGAGATG
TTCCTGGGCAGGCTGTTGGCACTCAGCTCCAGGAACAGCAGAAGGCTGGGCATCGTCAAA
GAGGATCACCTCAGGTGCAGGCAGGACCTGGCACTCCAGGACGCCGCCCACAAAACACGC
ATGAAGGCAAACACTGTGAAGTGCATGGAAGTGTTGGAAGGTCAGCTGAGCAGCTGCAGG
TGCCCGCTGCTTGGGAGAGTGTGGAAAGCAGACTTGGAGACTTGTCAGTTGCTAATGCAG
AGCCTGCAGCTTCAGGAAGCAGGCAGCAGCCCACACGCAGAGGACGAGGAGCAGGTGCAT
AGCACAGGAGAGGCCGCCCAGACAGCTGCTCTGGCTGTCCCTCGAACACCCCACCCCGAA
GAAGAAAAGTCCCCCTTGCAGGTGCTCCAGGAGTGGGACACCCACTCAGCTCTTTCACCA
CACTGTGCTGCAGGCCCATGGAAAGAGGATTCTCACATCGTTTCTGCTGAAGTTGGAGAA
AAGTGCGAAGCCATAGGCGTGAAGCTCCTACACCTGGAAGACCAGCTTCTCGGAGCCATG
TACAGTCACGATGAGGCTCTCTTTCAGTCTCTCCAGGGGGAGCTCCAGACGGTGAAGGAA
ACACTGCAGGCCATGATCCTGCAGCTCCAGCCAACAAAGGAGGCAGGAGAGGCCTCAGCT
TCCTATCCGACAGCTGGTGCTCAGGAAACCGAGGCC
Protein sequence predicted based on nucleotide sequence
MQGGGPRGAPIHSPSHGADSGHGLPPAVAPQRRRLTRRPGYMRSTAGSGIGFLSPAVGMP HPSSAGLTGQQSQHSQSKAGQCGLDPGSHCQASLVGKPFLKSSLVPAVASEGHLHPAQRS MRKRPVHFAVHSKNDSRQSERLTGSFKPGDSGFWQELLSSDSFKSLAPSLDAPWNKGSRG LKTVKPLASPALNGPADIASLPGFQDTFTSSFSFIQLSLGAAGERGEAEGCLPSREAEPL HQRPQEMAAEASSSDRPHGDPRHLWTFSLHAAPGLADLAQVTRSSSRQSECGTVSSSSSD TGFSSQDASSAGGRGDQGGGWADAHGWHTLLREWEPMLQDYLLSNRRQLEVTSLILKLQK CQEKVVEDGDYDTAETLRQRLEELEQEKGRLSWALPSQQPALRSFLGYLAAQIQVALHGA TQRAGSDDPEAPLEGQLRTTAQDSLPASITRRDWLIREKQRLQKEIEALQARMSALEAKE KRLSQELEEQEVLLRWPGCDLMALVAQMSPGQLQEVSKALGETLTSANQAPFQVEPPETL RSLRERTKSLNLAVRELTAQVCSGEKLCSSLRRRLSDLDTRLPALLEAKMLALSGSCFST AKELTEEIWALSSEREGLEMFLGRLLALSSRNSRRLGIVKEDHLRCRQDLALQDAAHKTR MKANTVKCMEVLEGQLSSCRCPLLGRVWKADLETCQLLMQSLQLQEAGSSPHAEDEEQVH STGEAAQTAALAVPRTPHPEEEKSPLQVLQEWDTHSALSPHCAAGPWKEDSHIVSAEVGE KCEAIGVKLLHLEDQLLGAMYSHDEALFQSLQGELQTVKETLQAMILQLQPTKEAGEASA SYPTAGAQETEA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2594
Start on reference sequence
36
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
