Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
HsCD00039757
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00836342
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3300nts
Open reading frame: 1 to 3300
ATGTTCGGCTCCGCCCCCCAGCGTCCCGTGGCCATGACGACCGCTCAGAGGGACTCCCTG
TTGTGGAAGCTCGCGGGGTTGCTGCGGGAGTCCGGGGATGTGGTCCTGTCTGGCTGTAGC
ACCCTGAGCCTGCTGACTCCCACACTGCAACAGCTGAACCACGTATTTGAGCTGCACCTG
GGGCCATGGGGCCCTGGCCAGACAGGCTTTGTGGCTCTGCCCTCCCATCCTGCCGACTCC
CCTGTTATTCTTCAGCTTCAGTTTCTCTTCGATGTGCTGCAGAAAACACTTTCACTCAAG
CTGGTCCATGTTGCTGGTCCTGGCCCCACAGGGCCCATCAAGATTTTCCCCTTCAAATCC
CTTCGGCACCTGGAGCTCCGAGGTGTTCCCCTCCACTGTCTGCATGGCCTCCGAGGCATC
TACTCCCAGCTGGAGACCCTGATTTGCAGCAGGAGCCTCCAGGCATTAGAGGAGCTCCTC
TCAGCCTGCGGCGGCGACTTCTGCTCTGCCCTCCCTTGGCTGGCTCTGCTTTCTGCCAAC
TTCAGCTACAATGCACTGACCGCCTTAGACAGCTCCCTGCGCCTCTTGTCAGCTCTGCGT
TTCTTGAACCTAAGCCACAATCAAGTCCAGGACTGTCAGGGATTCCTGATGGATTTGTGT
GAGCTCCACCATCTGGACATCTCCTATAATCGCCTGCATTTGGTGCCAAGAATGGGACCC
TCAGGGGCTGCTCTGGGGGTCCTGATACTGCGAGGCAATGAGCTTCGGAGCCTGCATGGC
CTAGAGCAGCTGAGGAATCTGCGGCACCTGGATTTGGCATACAACCTGCTGGAAGGACAC
CGGGAGCTGTCACCACTGTGGCTGCTGGCTGAGCTCCGCAAGCTCTACCTGGAGGGGAAC
CCTCTTTGGTTCCACCCTGAGCACCGAGCAGCCACTGCCCAGTACTTGTCACCCCGGGCC
AGGGATGCTGCTACTGGCTTCCTTCTCGATGGCAAGGTCTTGTCACTGACAGATTTTCAG
ACTCACACATCCTTGGGGCTCAGCCCCATGGGCCCACCTTTGCCCTGGCCAGTGGGGAGT
ACTCCTGAAACCTCAGGTGGCCCTGACCTGAGTGACAGCCTCTCCTCAGGGGGTGTTGTG
ACCCAGCCCCTGCTTCATAAGGTTAAGAGCCGAGTCCATGTGAGGCGGGCAAGCATCTCT
GAACCCAGTGATACGGACCCGGAGCCCGGAACTCTGAACCCCTCTCCGGCTGGATGGTTC
GTGCAGCAGCACCCGGAGCTGGAGCTCATGAGCAGCTTCCGGGAACGGTTCGGCCGCAAC
TGGCTGCAGTACAGGAGTCACCTGGAGCCCTCCGGAAACCCTCTGCCGGCCACCCCCACT
ACTTCTGCACCCAGTGCACCTCCAGCCAGCTCCCAGGGCCCCGACACTGCACCCAGACCT
TCACCCCCGCAGGAGGAAGCCAGAGGCCCCCAGGAGTCACCACAGAAAATGTCAGAGGAG
GTCAGGGCAGAGCCACAGGAGGAGGAAGAGGAGAAGGAGGGGAAGGAGGAGAAGGAGGAG
GGGGAGATGGTGGAACAGGGAGAAGAGGAGGCAGGAGAGGAGGAAGAAGAGGAGCAGGAC
CAGAAGGAAGTGGAAGCGGAACTCTGTCGCCCCTTGTTGGTGTGTCCCCTGGAGGGGCCT
GAGGGCATACGGGGCAGGGAATGCTTTCTCAGGGTCACTTCTGCCCACCTGTTTGAGGTG
GAACTCCAAGCAGCTCGCACCTTGGAGCGACTGGAGCTCCAGAGTCTGGAGGCAGCTGAG
ATAGAGCCGGAGGCCCAGGCCCAGAGGTCGCCCAGGCCCACGGGCTCAGATCTGCTCCCT
GGAGCCCCCATCCTCAGTCTGCGCTTCTCCTACATCTGCCCTGACCGGCAGTTGCGTCGC
TATTTGGTGCTGGAGCCTGATGCCCACGCAGCTGTCCAGGAGCTGCTTGCCGTGTTGACC
CCAGTCACCAATGTGGCTCGGGAACAGCTTGGGGAGGCCAGGGACCTCCTGCTGGGTAGA
TTCCAGTGTCTACGCTGTGGCCATGAGTTCAAGCCAGAGGAGCCCAGGATGGGATTAGAC
AGTGAGGAAGGCTGGAGGCCTCTGTTCCAAAAGACAGAATCTCCTGCTGTGTGTCCTAAC
TGTGGTAGTGACCACGTGGTTCTCCTCGCTGTGTCTCGGGGAACCCCCAACAGGGAGCGG
AAACAGGGAGAGCAGTCTCTGGCTCCTTCTCCGTTTGCCAGCCCTGTCTGCCACCCTCCT
GGCCATGGTGACCACCTTGACAGGGCCAAGAACAGCCCACCTCAGGCACCGAGCACCCGT
GACCATGGTAGTTGGAGCCTCAGTCCCCCCCCTGAGCGCTGTGGCCTCCGCTCTGTGGAC
CACCGACTCCGGCTCTTCCTGGATGTTGAGGTGTTCAGCGATGCCCAGGAGGAGTTCCAG
TGCTGCCTCAAGGTGCCAGTGGCATTGGCAGGCCACACTGGGGAGTTCATGTGCCTTGTG
GTTGTGTCTGACCGCAGGCTGTACCTGTTGAAGGTGACTGGGGAGATGCGTGAGCCTCCA
GCTAGCTGGCTGCAGCTGACCCTGGCTGTTCCCCTGCAGGATCTGAGTGGCATAGAGCTG
GGCCTGGCAGGCCAGAGCCTGCGGCTAGAGTGGGCAGCTGGGGCGGGCCGCTGTGTGCTG
CTGCCCCGAGATGCCAGGCATTGCCGGGCCTTCCTAGAGGAGCTCCTTGATGTCTTGCAG
TCTCTGCCCCCTGCCTGGAGGAACTGTGTCAGTGCCACAGAGGAGGAGGTCACCCCCCAG
CACCGGCTCTGGCCATTGCTGGAAAAAGACTCATCCTTGGAGGCTCGCCAGTTCTTCTAC
CTTCGGGCGTTCCTGGTTGAAGGCCCTTCCACCTGCCTCGTATCCCTGTTGCTGACTCCG
TCCACCCTGTTCCTGTTAGATGAGGATGCTGCAGGGTCCCCGGCAGAGCCCTCTCCTCCA
GCAGCATCTGGCGAAGCCTCTGAGAAGGTGCCTCCCTCGGGGCCGGGCCCTGCTGTGCGT
GTCAGGGAGCAGCAGCCACTCAGCAGCCTGAGCTCCGTGCTGCTCTACCGCTCAGCCCCT
GAGGACTTGCGGCTGCTCTTCTACGATGAGGTGTCCCGGCTGGAGAGCTTTTGGGCACTC
CGTGTGGTGTGTCAGGAGCAGCTGACAGCCCTGCTTGCCTGGATCCGGGAACCATGGGAG
GAGCTGTTTTCCGTCGGACTCCGGACAGTGATCCAAGAGGCGCTGGCCCTTGACCGATTG
Protein sequence predicted based on nucleotide sequence
MFGSAPQRPVAMTTAQRDSLLWKLAGLLRESGDVVLSGCSTLSLLTPTLQQLNHVFELHL GPWGPGQTGFVALPSHPADSPVILQLQFLFDVLQKTLSLKLVHVAGPGPTGPIKIFPFKS LRHLELRGVPLHCLHGLRGIYSQLETLICSRSLQALEELLSACGGDFCSALPWLALLSAN FSYNALTALDSSLRLLSALRFLNLSHNQVQDCQGFLMDLCELHHLDISYNRLHLVPRMGP SGAALGVLILRGNELRSLHGLEQLRNLRHLDLAYNLLEGHRELSPLWLLAELRKLYLEGN PLWFHPEHRAATAQYLSPRARDAATGFLLDGKVLSLTDFQTHTSLGLSPMGPPLPWPVGS TPETSGGPDLSDSLSSGGVVTQPLLHKVKSRVHVRRASISEPSDTDPEPGTLNPSPAGWF VQQHPELELMSSFRERFGRNWLQYRSHLEPSGNPLPATPTTSAPSAPPASSQGPDTAPRP SPPQEEARGPQESPQKMSEEVRAEPQEEEEEKEGKEEKEEGEMVEQGEEEAGEEEEEEQD QKEVEAELCRPLLVCPLEGPEGIRGRECFLRVTSAHLFEVELQAARTLERLELQSLEAAE IEPEAQAQRSPRPTGSDLLPGAPILSLRFSYICPDRQLRRYLVLEPDAHAAVQELLAVLT PVTNVAREQLGEARDLLLGRFQCLRCGHEFKPEEPRMGLDSEEGWRPLFQKTESPAVCPN CGSDHVVLLAVSRGTPNRERKQGEQSLAPSPFASPVCHPPGHGDHLDRAKNSPPQAPSTR DHGSWSLSPPPERCGLRSVDHRLRLFLDVEVFSDAQEEFQCCLKVPVALAGHTGEFMCLV VVSDRRLYLLKVTGEMREPPASWLQLTLAVPLQDLSGIELGLAGQSLRLEWAAGAGRCVL LPRDARHCRAFLEELLDVLQSLPPAWRNCVSATEEEVTPQHRLWPLLEKDSSLEARQFFY LRAFLVEGPSTCLVSLLLTPSTLFLLDEDAAGSPAEPSPPAASGEASEKVPPSGPGPAVR VREQQPLSSLSSVLLYRSAPEDLRLLFYDEVSRLESFWALRVVCQEQLTALLAWIREPWE ELFSVGLRTVIQEALALDRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
