Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
HsCD00039954
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00836442
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3471nts
Open reading frame: 1 to 3471
ATGTTCCCAGCCGCCCCTTCTCCGCGGACCCCGGGTACCGGGTCCCGAAGGGGCCCGCTG
GCCGGACTCGGGCCCGGCTCCACGCCCCGGACGGCTAGCAGGAAGGGTCTGCCCCTGGGG
TCTGCAGTCAGCTCCCCAGTGCTCTTCTCGCCGGTCGGCCGGCGTAGCTCGCTAAGCTCG
CGGGGAACACCAACACGAATGTTCCCACACCACTCCATAACTGAGTCTGTGAACTATGAT
GTGAAAACGTTTGGATCTTCTCTTCCTGTTAAAGTCATGGAAGCCCTAACATTGGCTGAA
GTCGATGACCAGCTGACCATTAACATAGATGAAGGTGGATGGGCTTGTCTGGTGTGCAAA
GAGAAGCTCATTATTTGGAAGATTGCTCTGTCACCTATTACTAAGTTATCCGTTTGCAAA
GAACTTCAGCTGCCACCTAGTGATTTCCACTGGAGTGCCGACTTAGTGGCTCTTTCTTAC
TCTTCTCCCTCAGGTGAAGCACATTCTACTCAGGCTGTTGCTGTCATGGTTGCCACCAGA
GAAGGATCTATCCGCTATTGGCCAAGCCTTGCTGGTGAAGATACCTACACAGAGGCTTTT
GTAGATTCGGGAGGTGATAAGACTTACAGTTTCCTAACAGCAGTGCAGGGAGGAAGTTTT
ATTTTGTCTTCATCAGGAAGCCAACTAATTCGGTTGATACCTGAGAGCTCAGGAAAGATT
CATCAGCATATCCTGCCTCAGGGGCAAGGCATGCTTTCAGGAATTGGTCGAAAAGTTTCT
TCTCTTTTTGGAATTTTATCTCCTAGTAGTGATCTCACACTTTCAAGTGTTCTCTGGGAT
AGAGAGAGATCAAGCTTTTATAGCCTGACGAGTTCAAACATCAGTAAATGGGAATTAGAT
GATTCTTCAGAAAAGCATGCATACAGTTGGGATATAAATAGAGCCCTGAAGGAAAACATT
ACCGATGCTATTTGGGGATCTGAAAGTAACTATGAAGCTATTAAAGAAGGAGTCAACATT
CGATATTTGGACTTTAAGCAAAACTGTGATGGGCTGGTGATTTTGGCAGCAGCATGGCAC
TCAGCAGACAATCCATGTCTCATCTATTACTCTCTGATAACAATAGAAGATAATGGTTGC
CAAATGTCAGATGCAGTTACTGTAGAAGTCACTCAATATAATCCACCTTTTCAGTCTGAA
GACCTGATTTTGTGTCAGTTGACGGTCCCAAACTTTTCAAACCAGACTGCCTATCTGTAT
AACGAAAGTGCTGTCTATGTGTGCTCCACAGGAACTGGGAAATTTTCTCTTCCCCAGGAG
AAAATTGTCTTTAATGCACAAGGAGATAGTGTTTTAGGTGCTGGTGCCTGTGGTGGTGTT
CCTATCATTTTTTCTAGAAACAGTGGACTGGTGTCTATTACTTCAAGGGAAAATGTGTCT
ATATTGGCAGAAGACTTGGAAGGGTCTTTAGCATCTTCAGTTGCTGGACCAAACAGTGAG
AGTATGATTTTTGAGACCACTACAAAGAATGAAACTATAGCCCAGGAAGATAAAATCAAG
TTGCTGAAAGCTGCCTTTCTGCAATACTGCAGAAAAGATTTAGGTCATGCTCAAATGGTG
GTTGATGAGCTCTTTTCCTCTCACTCTGATTTGGATTCTGATTCTGAACTAGACAGGGCA
GTTACCCAAATCAGTGTAGACCTGATGGATGACTACCCAGCATCTGACCCACGGTGGGCT
GAGTCTGTCCCTGAGGAAGCACCTGGGTTCAGCAATACGTCACTGATTATCCTTCACCAG
CTAGAAGACAAGATGAAAGCTCACTCTTTTCTTATGGACTTTATTCATCAAGTTGGCTTA
TTTGGACGTCTAGGCAGTTTTCCAGTTAGAGGGACACCGATGGCCACTCGACTGTTGCTC
TGTGAGCATGCCGAAAAGCTGTCAGCCGCCATTGTTCTCAAGAACCACCACTCCCGGCTT
TCTGACCTTGTCAACACAGCCATATTGATTGCTTTGAACAAGAGGGAGTATGAAATCCCA
TCCAACCTGACTCCTGCAGATGTCTTTTTCAGGGAGGTATCCCAAGTAGATACCATCTGT
GAGTGCTTACTGGAGCATGAGGAGCAAGTCTTGAGGGATGCACCTATGGATTCCATTGAA
TGGGCTGAAGTGGTGATCAATGTGAACAATATTCTCAAGGATATGCTGCAGGCTGCTAGT
CATTATCGCCAAAATAGAAACTCTTTGTATAGAAGAGAAGAATCACTAGAAAAAGAACCT
GAATATGTTCCATGGACGGCAACAAGTGGTCCTGGTGGCATCCGAACGGTAATAATACGC
CAGCATGAGATTGTCCTGAAGGTGGCTTATCCACAGGCAGACAGCAACCTCCGAAACATC
GTGACCGAGCAGCTGGTAGCCCTGATCGATTGCTTCCTGGATGGTTATGTTTCTCAGCTT
AAGTCTGTGGATAAATCCAGTAATCGGGAAAGATATGACAATCTGGAGATGGAATACCTA
CAGAAAAGATCAGATCTCTTATCTCCTCTTCTTTCACTAGGCCAGTACCTGTGGGCTGCT
TCTCTAGCAGAGAAATACTGTGACTTTGATATATTGGTACAAATGTGTGAGCAGACTGAC
AACCAGAGCCGACTCCAGCGCTACATGACCCAGTTTGCTGATCAGAATTTTTCAGACTTT
CTCTTCCGTTGGTATCTGGAGAAAGGAAAGCGAGGCAAATTATTATCTCAGCCCATTTCT
CAGCATGGACAGTTGGCAAATTTTTTGCAAGCTCATGAACATCTCAGCTGGTTACATGAA
ATTAATAGCCAAGAATTAGAAAAGGCTCATGCAACACTTCTGGGTTTGGCAAATATGGAA
ACTCGTTACTTTGCAAAGAAGAAAACCCTTCTTGGCTTGAGTAAATTGGCTGCATTAGCT
TCAGACTTTTCAGAGGATATGCTACAAGAAAAAATTGAAGAAATGGCTGAGCAGGAGCGC
TTTCTACTGCATCAGGAGACCCTACCTGAACAGCTGCTGGCGGAGAAACAGCTAAATCTC
AGTGCGATGCCAGTATTGACTGCACCACAACTCATTGGTCTATATATCTGTGAAGAAAAT
AGAAGAGCTAATGAATATGATTTCAAGAAAGCTTTGGACTTGTTGGAATATATTGATGAG
GAAGAAGATATAAATATAAATGATCTAAAACTGGAAATCCTTTGCAAAGCTCTTCAGAGA
GATAACTGGTCCAGTTCTGATGGCAAAGATGATCCAATTGAAGTATCTAAAGACAGTATA
TTTGTGAAGATCTTACAGAAACTTTTAAAAGATGGCATTCAGCTCAGTGAGTACTTACCG
GAGGTGAAAGACCTGCTACAAGCGGATCAGCTTGGAAGCTTAAAGTCCAATCCTTACTTC
GAGTTTGTTTTGAAAGCAAATTATGAATATTATGTTCAGGGACAAATATTG
Protein sequence predicted based on nucleotide sequence
MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSS RGTPTRMFPHHSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCK EKLIIWKIALSPITKLSVCKELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATR EGSIRYWPSLAGEDTYTEAFVDSGGDKTYSFLTAVQGGSFILSSSGSQLIRLIPESSGKI HQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWDRERSSFYSLTSSNISKWELD DSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDFKQNCDGLVILAAAWH SADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLY NESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVS ILAEDLEGSLASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMV VDELFSSHSDLDSDSELDRAVTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQ LEDKMKAHSFLMDFIHQVGLFGRLGSFPVRGTPMATRLLLCEHAEKLSAAIVLKNHHSRL SDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTICECLLEHEEQVLRDAPMDSIE WAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIR QHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYL QKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDF LFRWYLEKGKRGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANME TRYFAKKKTLLGLSKLAALASDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNL SAMPVLTAPQLIGLYICEENRRANEYDFKKALDLLEYIDEEEDININDLKLEILCKALQR DNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYF EFVLKANYEYYVQGQIL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
