DNASU Plasmid | TM0086 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0086

Gene Name: virulence factor MviN-related protein

Original Clone ID:

7040

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Next amino acid codon after CDS represents STOP, hence clone should be labeled for CDS 'fusion', overall 'closed'

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00083937

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1461nts

Open reading frame: 37 to 1497

ATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTCCAGCATAAAGAAAACGCTC
GCTTTCTCTCTGGGAACCTTTTTCTCCCGTATCACGGGTCTCGTTCGTGATATAATTCTC
GCAAAAACCTTCGGTGCTTCCTCTACTCTCGATGCCTACTATGTTTCCATAGTCTTTCCG
TTCTTCCTCAGGAGGACCTTCGCTGAGGGTGCGATGAGCTCAGCCTTCATGGCAATCTAC
AAAAAGCTGAAGAACAAAGAAGAAAAAGCACAATTCACCTCGGCTGTGCTCACCTCTCTT
GGACTCGTTACTCTCCTCATTGTCTTTCTTTCCGAAGTTTTTCCTTACTTCATGGCATCT
ATCTTCGCCACAGGTGCCGACGAAGAAGTGAAATCGCTGGCAGCCGATCTCATACGCCTG
ACAGCTCCATTCATAACCATCGTTTTTGTGTGGGCAGTTTTTTATTCGGTACACAACGCC
TCACACAGATACTTCCTTCCCGCGTTGACTCCGATGTTTTCGAACGTAGGTGTGATAGTG
GGATGTCTATTCGGTGATGTAAGGTGGGCAGCCGCCGGATTCACGATAGGAGGTCTGGCT
GCACTACTGGTTTTACTTCCCTTCGGAAAATTTCGATACAGACCAACATTCAAAGGCCTT
GGAGAATTCTACAGACTCTTCTTTGGAACGTTTATGACAATGGCAGTCTCTCAAGTAACC
ACACTGATCGATGTGAACGTTGCCTCTTTTTTAGACCCAGGTTCTCTCTCTTTGATCCAG
CTTTCCAGTCGTCTTTACCAGCTTCCACTCGGTATCTTTGGTGTTGCGGTATCCACCGTT
GCACTGTCCACCCTCAGCGAAAGCGAAGGGGATTTTCACGAGAATCTGAAAGATTTCATA
AGCAAAAGCCTCTTTCTGACGCTTCCTTCCTCGATTGGATTGATGGCTCTTTCCGAGAGA
ATCATTTCTCTACTTTTCGGATATGGAGCGTTCACCCATGAAGATGTGAAAAAATCGGCT
CAGATACTCTTCATGTACGCAATTGGATTGTGTTTTGTGTCCCTGTTTAACCTTTTATCC
CGAGCCTATCACGCTTCGAAAGAAGTGAAGACTCCCTTCTTTGCCACTCTGCTCGTTTCT
GCTGTGAACATATCGCTCGACGTGATACTCGGTTTCACCATGGGAGCCTCGGGCATCGCA
CTTGCGACGAGTGTTTCATACATTGCAGGATTCGTTTTTCTCACTTTAAGGATGAAACCT
TCCTTCGACAAAAAGATTTTCAAAATCAGCCTGGCTTCTGCGGTCATGGGAACTGTGATT
CTTCTGCTGAGAGGTTCATTCAAAGGGAATCTCGGAACGATCTTTCTGGTACTGATCGGT
GTGTTCGTTTATGTGTTGTTTTCGAAACTTCTGAAAATAGAAGAACTCGAAGAGATTCTC
AGAAGAGGATCGCACTAATTAANNNNNNNNNNNNNNNNNNNNNNNCTACCCGGGTAAGTT
TAAACGGTCTCCAG

Protein sequence predicted based on nucleotide sequence

VSSIKKTLAFSLGTFFSRITGLVRDIILAKTFGASSTLDAYYVSIVFPFFLRRTFAEGAM
SSAFMAIYKKLKNKEEKAQFTSAVLTSLGLVTLLIVFLSEVFPYFMASIFATGADEEVKS
LAADLIRLTAPFITIVFVWAVFYSVHNASHRYFLPALTPMFSNVGVIVGCLFGDVRWAAA
GFTIGGLAALLVLLPFGKFRYRPTFKGLGEFYRLFFGTFMTMAVSQVTTLIDVNVASFLD
PGSLSLIQLSSRLYQLPLGIFGVAVSTVALSTLSESEGDFHENLKDFISKSLFLTLPSSI
GLMALSERIISLLFGYGAFTHEDVKKSAQILFMYAIGLCFVSLFNLLSRAYHASKEVKTP
FFATLLVSAVNISLDVILGFTMGASGIALATSVSYIAGFVFLTLRMKPSFDKKIFKISLA
SAVMGTVILLLRGSFKGNLGTIFLVLIGVFVYVLFSKLLKIEELEEILRRGSH*LLPG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1422

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0086

GENEID :

896913

Locus Tag :

TM0086

Target GenBank:

NP_227902

Protein

Structural Annotation Wiki :

TOPSAN

Clone

Cloning Information :

271565

HIP Master Clone ID :

202275

Original Clone ID :

7040

Experimental

TargetTrack - Experimental data :

281967

Organism

Species Specific ID:

896913