Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840045
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6120nts
Open reading frame: 1 to 6120
ATGGAACATAAGGAAGTGGTTCTTCTACTTCTCTTATTTCTGAAATCAGCAGCACCTGAG
CAGAGCCATGTCGTGCAGGATTGTTATCATGGAGATGGGCAAAGTTACCGAGGCACGTAC
AGTACCACAGTAACCGGAAGGACGTGCCAAGCGTGGTCTAGCATGACACCCCACCAACAT
AATAGGACAACAGAAAATTATCCCAATGCAGGCTTAATAATGAATTACTGCCGAAATCCA
GACGCTGTTGCTGCTCCATACTGTTACACTAGAGATCCTGGTGTTAGATGGGAGTACTGC
AACTTGACGCAATGTTCAGACGCAGAGGGAACCGCCGTGGCGCCCCCCACAGTTACCCCC
GTCCCAAGCCTAGAGGCTCCTTCAGAGCAAGCTCCAACCGAACAAAGGCCCGGCGTGCAA
GAATGCTATCATGGGAACGGTCAGAGTTACCGCGGAACATATTCGACCACTGTTACGGGA
CGGACCTGTCAAGCTTGGTCTTCAATGACACCTCATTCGCATTCACGGACCCCTGAATAT
TATCCAAACGCTGGGCTTATCATGAATTATTGCAGAAACCCAGATGCTGTGGCAGCCCCT
TACTGTTATACGAGGGATCCCGGTGTCAGGTGGGAGTACTGCAACCTGACTCAGTGCTCC
GACGCCGAAGGCACTGCTGTCGCGCCTCCTACCGTTACTCCGGTTCCATCCCTAGAAGCC
CCTTCGGAGCAAGCCCCGACAGAACAACGGCCCGGGGTACAGGAATGTTATCATGGTAAT
GGGCAATCCTATCGCGGAACCTATTCCACCACTGTGACAGGACGTACCTGCCAAGCATGG
TCATCAATGACGCCACACTCACACAGTCGCACCCCGGAGTACTACCCGAATGCTGGGTTA
ATTATGAATTACTGTCGCAACCCAGACGCGGTCGCAGCTCCCTATTGCTACACGAGAGAT
CCAGGTGTCCGCTGGGAGTACTGCAACCTCACTCAGTGTTCCGACGCAGAAGGGACAGCT
GTTGCGCCTCCGACTGTGACTCCCGTTCCTAGTCTAGAGGCGCCTAGTGAGCAAGCGCCG
ACCGAGCAGAGGCCTGGGGTGCAAGAGTGCTACCACGGAAATGGACAGAGTTATCGGGGA
ACATACTCCACTACAGTCACGGGGAGAACTTGTCAGGCCTGGAGCTCAATGACTCCACAC
TCCCACAGTAGAACACCGGAATACTACCCCAACGCTGGCCTGATTATGAACTACTGTAGG
AATCCAGATGCAGTGGCAGCACCATATTGTTATACTCGCGATCCCGGCGTTAGGTGGGAG
TATTGTAACCTGACGCAGTGCAGCGATGCCGAGGGCACCGCCGTCGCGCCCCCGACCGTA
ACACCCGTTCCAAGTCTCGAAGCGCCTTCCGAACAAGCTCCCACTGAACAAAGACCAGGG
GTTCAGGAGTGTTATCATGGCAACGGCCAGTCTTATCGCGGCACCTATTCAACCACCGTT
ACCGGACGGACATGCCAAGCCTGGTCATCTATGACCCCACACTCGCATAGTCGCACACCA
GAATACTATCCCAACGCCGGATTGATCATGAACTACTGCCGTAATCCTGATGCCGTGGCA
GCTCCTTATTGCTATACAAGGGACCCAGGTGTGAGGTGGGAGTACTGTAACTTGACACAG
TGTTCGGACGCTGAAGGGACTGCCGTCGCCCCCCCGACGGTTACCCCGGTGCCCAGCCTG
GAAGCTCCCTCCGAACAGGCTCCGACAGAGCAAAGACCTGGCGTACAGGAGTGCTATCAC
GGGAATGGACAATCCTACAGAGGCACCTACAGCACCACAGTCACAGGCCGCACTTGCCAG
GCCTGGTCCTCTATGACTCCCCACTCTCATAGTAGGACTCCAGAGTATTACCCGAACGCT
GGATTGATAATGAACTACTGCCGCAACCCGGATGCAGTCGCAGCCCCCTACTGCTATACT
AGGGATCCGGGTGTAAGATGGGAGTACTGCAATCTGACTCAATGCTCCGATGCAGAGGGG
ACCGCAGTCGCGCCACCGACTGTTACCCCTGTTCCTAGCCTTGAGGCCCCCTCTGAACAG
GCACCGACGGAGCAGCGGCCAGGGGTGCAGGAATGCTACCATGGGAATGGTCAAAGTTAT
AGAGGCACATATTCCACAACCGTTACAGGCAGAACCTGCCAGGCGTGGAGCTCGATGACA
CCGCACTCCCATAGTCGGACCCCCGAGTACTATCCTAATGCTGGCTTAATCATGAACTAT
TGTCGGAATCCCGATGCGGTGGCCGCCCCATACTGCTACACGCGCGATCCTGGGGTAAGG
TGGGAGTACTGCAACCTAACACAATGCTCGGACGCGGAAGGCACAGCCGTTGCGCCACCC
ACCGTAACTCCTGTTCCCAGCCTCGAAGCACCTTCAGAACAGGCGCCCACAGAGCAGCGA
CCTGGAGTTCAAGAGTGTTACCACGGCAACGGGCAGAGCTACCGAGGAACGTACTCAACA
ACTGTCACTGGGAGAACGTGTCAGGCTTGGTCAAGCATGACGCCCCATTCGCACTCACGA
ACCCCCGAATATTACCCCAATGCCGGCCTCATCATGAACTACTGCAGGAACCCAGATCCT
GTCGCCGCCCCTTATTGTTACACCAGGGATCCAAGTGTCCGTTGGGAGTACTGCAATTTA
ACCCAATGTTCTGATGCAGAAGGTACCGCCGTAGCGCCTCCAACCATTACCCCGATTCCC
AGTCTGGAGGCTCCCTCGGAACAAGCGCCCACTGAGCAAAGGCCGGGCGTCCAAGAATGT
TATCACGGAAACGGACAGAGCTATCAAGGAACCTACTTCATTACCGTCACGGGACGAACC
TGTCAGGCGTGGTCAAGTATGACGCCTCACTCGCACAGTCGTACCCCAGCCTACTATCCG
AACGCCGGCTTGATCAAAAATTACTGTAGAAATCCCGATCCTGTGGCTGCCCCTTGGTGT
TACACAACAGATCCCAGCGTGAGGTGGGAATATTGTAATCTGACGCGATGCTCAGATGCC
GAATGGACTGCCTTCGTCCCTCCTAATGTTATTCTGGCCCCATCCCTCGAGGCTTTTTTT
GAACAAGCACTGACTGAGGAAACCCCCGGCGTACAAGACTGCTACTACCATTATGGACAA
AGCTACCGGGGGACATATTCTACCACTGTAACAGGAAGAACATGCCAGGCATGGTCTTCC
ATGACCCCTCACCAGCATAGCAGGACACCAGAGAATTACCCAAACGCAGGGCTGACCAGG
AACTACTGTCGGAACCCGGACGCAGAGATTCGCCCATGGTGTTACACGATGGACCCGAGT
GTCAGATGGGAGTATTGCAACCTTACTCAATGTCTCGTGACAGAATCAAGTGTCTTGGCA
ACTCTCACCGTCGTGCCAGATCCATCAACAGAGGCTTCATCCGAAGAAGCTCCTACGGAG
CAAAGCCCAGGCGTCCAGGATTGCTACCATGGTGATGGCCAATCTTATCGAGGGTCATTC
TCTACCACCGTGACAGGGAGAACATGTCAGTCCTGGTCCAGTATGACCCCGCATTGGCAC
CAGCGCACAACCGAGTATTATCCGAATGGTGGCTTGACAAGAAATTATTGTCGCAATCCC
GACGCGGAGATTAGTCCGTGGTGTTATACAATGGATCCCAATGTGAGATGGGAATACTGT
AATTTGACCCAGTGTCCAGTTACAGAATCTAGTGTCCTTGCGACGTCCACGGCTGTTTCC
GAGCAAGCACCAACGGAACAATCACCCACAGTCCAAGACTGTTATCATGGCGATGGACAG
TCTTACCGTGGCTCATTCTCCACCACGGTCACCGGAAGAACGTGCCAGTCGTGGTCTTCT
ATGACACCACACTGGCATCAAAGAACTACCGAATACTACCCTAATGGCGGACTGACCCGC
AATTATTGCCGTAACCCCGATGCTGAGATTAGGCCATGGTGCTACACAATGGACCCCAGT
GTACGTTGGGAATATTGCAATCTTACGCAGTGTCCCGTCATGGAGTCAACTCTTCTCACA
ACCCCCACCGTGGTCCCAGTTCCAAGCACAGAGCTGCCTTCTGAAGAAGCGCCAACTGAA
AACAGCACCGGTGTGCAGGACTGCTATCGTGGTGACGGACAGTCATATCGAGGCACACTC
TCCACAACAATTACAGGACGAACTTGCCAATCATGGTCGTCAATGACCCCCCACTGGCAC
CGGAGGATCCCACTCTATTATCCTAACGCGGGCCTGACCAGAAACTACTGCCGGAATCCT
GACGCCGAGATTCGGCCGTGGTGCTATACCATGGATCCTAGTGTGCGGTGGGAGTACTGT
AACCTCACAAGATGTCCAGTGACCGAATCGAGTGTCCTCACAACTCCGACCGTGGCCCCC
GTGCCATCTACAGAAGCACCCTCAGAACAAGCCCCACCCGAGAAAAGCCCTGTGGTCCAA
GATTGCTATCACGGTGATGGACGGAGCTATCGGGGCATATCCTCCACGACCGTCACAGGA
AGGACCTGCCAAAGTTGGTCCTCAATGATACCACATTGGCATCAGAGGACCCCGGAAAAC
TATCCAAATGCTGGACTTACCGAGAACTATTGCCGCAATCCGGACTCTGGGAAACAACCT
TGGTGCTACACCACCGATCCGTGTGTGCGATGGGAGTACTGTAATCTCACACAATGTTCC
GAAACAGAATCCGGTGTCCTAGAGACTCCCACGGTTGTTCCAGTGCCCTCTATGGAGGCC
CATTCCGAAGCTGCACCAACCGAGCAAACGCCTGTGGTTCGGCAATGTTACCATGGCAAT
GGCCAAAGCTATCGAGGAACATTCTCAACCACAGTGACGGGAAGGACATGTCAATCGTGG
TCGTCCATGACTCCTCACAGGCATCAGCGAACCCCAGAAAACTACCCAAATGATGGCCTT
ACGATGAATTACTGCAGAAATCCGGATGCCGATACAGGGCCTTGGTGTTTTACTATGGAC
CCTAGCATTCGCTGGGAATACTGCAATCTCACGCGGTGCTCAGACACAGAAGGAACTGTT
GTCGCTCCCCCGACTGTCATTCAGGTGCCAAGTCTAGGGCCTCCTAGTGAACAGGACTGT
ATGTTTGGAAATGGGAAAGGATACCGGGGCAAAAAGGCCACCACCGTCACCGGGACGCCA
TGTCAAGAATGGGCTGCCCAGGAGCCCCATAGACACAGCACGTTCATTCCAGGGACAAAT
AAATGGGCAGGTCTGGAAAAAAACTATTGTAGAAACCCTGATGGTGACATCAATGGGCCC
TGGTGCTACACTATGAATCCAAGAAAACTGTTTGACTACTGTGATATCCCTCTCTGTGCA
TCCTCTTCCTTTGATTGTGGGAAGCCTCAAGTGGAGCCGAAGAAATGTCCTGGCAGCATT
GTAGGCGGGTGTGTGGCCCACCCTCATTCCTGGCCTTGGCAAGTCAGTCTGAGAACAAGG
TTTGGGAAGCACTTCTGTGGAGGCACTTTAATATCCCCAGAGTGGGTGCTGACTGCCGCT
CACTGCTTGAAGAAGTCCTCAAGACCTTCATCCTACAAGGTCATCCTCGGTGCACACCAA
GAAGTGAACCTCGAATCTCATGTCCAGGAAATAGAAGTGTCTAGGCTCTTCCTGGAGCCC
ACACAAGCAGATATTGCCTTGCTAAAACTAAGTAGGCCTGCCGTCATCACTGACAAAGTA
ATGCCAGCTTGTCTGCCTTCACCAGACTACATGGTCACCGCCAGAACTGAATGTTACATC
ACTGGCTGGGGCGAAACCCAAGGTACATTTGGGACTGGCCTTCTCAAGGAAGCCCAACTC
CTTGTTATTGAAAATGAAGTGTGCAATCACTATAAGTATATTTGTGCTGAGCATTTAGCC
AGAGGAACTGACTCTTGCCAGGGTGACAGCGGAGGGCCTCTGGTTTGCTTCGAAAAGGAC
AAGTACATTTTGCAAGGAGTCACTTCATGGGGTCTGGGCTGTGCACGCCCCAATAAGCCC
GGTGTTTATGCTCGTGTTTCCAGGTTTGTTACTTGGATTGAGGGGATGATGAGAAATAAT
Protein sequence predicted based on nucleotide sequence
MEHKEVVLLLLLFLKSAAPEQSHVVQDCYHGDGQSYRGTYSTTVTGRTCQAWSSMTPHQH NRTTENYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTP VPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEY YPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEA PSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGL IMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAP TEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCR NPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPG VQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVA APYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYH GNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYT RDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSY RGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVR WEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYST TVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDPVAAPYCYTRDPSVRWEYCNL TQCSDAEGTAVAPPTITPIPSLEAPSEQAPTEQRPGVQECYHGNGQSYQGTYFITVTGRT CQAWSSMTPHSHSRTPAYYPNAGLIKNYCRNPDPVAAPWCYTTDPSVRWEYCNLTRCSDA EWTAFVPPNVILAPSLEAFFEQALTEETPGVQDCYYHYGQSYRGTYSTTVTGRTCQAWSS MTPHQHSRTPENYPNAGLTRNYCRNPDAEIRPWCYTMDPSVRWEYCNLTQCLVTESSVLA TLTVVPDPSTEASSEEAPTEQSPGVQDCYHGDGQSYRGSFSTTVTGRTCQSWSSMTPHWH QRTTEYYPNGGLTRNYCRNPDAEISPWCYTMDPNVRWEYCNLTQCPVTESSVLATSTAVS EQAPTEQSPTVQDCYHGDGQSYRGSFSTTVTGRTCQSWSSMTPHWHQRTTEYYPNGGLTR NYCRNPDAEIRPWCYTMDPSVRWEYCNLTQCPVMESTLLTTPTVVPVPSTELPSEEAPTE NSTGVQDCYRGDGQSYRGTLSTTITGRTCQSWSSMTPHWHRRIPLYYPNAGLTRNYCRNP DAEIRPWCYTMDPSVRWEYCNLTRCPVTESSVLTTPTVAPVPSTEAPSEQAPPEKSPVVQ DCYHGDGRSYRGISSTTVTGRTCQSWSSMIPHWHQRTPENYPNAGLTENYCRNPDSGKQP WCYTTDPCVRWEYCNLTQCSETESGVLETPTVVPVPSMEAHSEAAPTEQTPVVRQCYHGN GQSYRGTFSTTVTGRTCQSWSSMTPHRHQRTPENYPNDGLTMNYCRNPDADTGPWCFTMD PSIRWEYCNLTRCSDTEGTVVAPPTVIQVPSLGPPSEQDCMFGNGKGYRGKKATTVTGTP CQEWAAQEPHRHSTFIPGTNKWAGLEKNYCRNPDGDINGPWCYTMNPRKLFDYCDIPLCA SSSFDCGKPQVEPKKCPGSIVGGCVAHPHSWPWQVSLRTRFGKHFCGGTLISPEWVLTAA HCLKKSSRPSSYKVILGAHQEVNLESHVQEIEVSRLFLEPTQADIALLKLSRPAVITDKV MPACLPSPDYMVTARTECYITGWGETQGTFGTGLLKEAQLLVIENEVCNHYKYICAEHLA RGTDSCQGDSGGPLVCFEKDKYILQGVTSWGLGCARPNKPGVYARVSRFVTWIEGMMRNN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
