Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840077
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3828nts
Open reading frame: 1 to 3828
ATGGTGGAGGCGGCGCCCCCCGGGCCCGGGCCGCTTCGGAGAACCTTTCTAGTGCCCGAG
ATTAAGTCGCTCGACCAGTACGATTTCTCGAGGGCCAAGGCGGCGGCCAGTCTTGCGTGG
GTGCTGAGGGCTGCGTTCGGCGGAGCAGAGCACGTCCCCCCGGAGCTGTGGGAGCCTTTC
TATACCGACCAGTACGCGCAGGAGCATGTCAAGCCCCCGGTGACACGGCTGCTGCTCTCT
GCCGAGCTCTACTGCAGAGCCTGGCGCCAGGCACTGCCACAGCTTGAAACACCTCCCAAC
CCCTCAGCACTGCTCGCCCTGCTTGCGCGGAGGGGCACAGTGCCTGCTTTGCCCGAGCGC
CCGGTGCGCGAGGCCGACCTGAGGCACCAGCCCATTCTCATGGGAGCCCACCTGGCTGTC
ATTGATGCCCTCATGGCTGCCTTTGCCTTCGAGTGGACCAAGACCCTGCCAGGTCCCTTG
GCCCTGACCAGCTTGGAGCACAAGCTGCTTTTCTGGGTGGACACGACAGTCCGGCGGTTG
CAGGAGAAGACCGAGCAGGAAGCGGCCCAGCGAGCCTCTCCAGCAGCCCCTGCAGACGGG
GCGGCCCCGGCTCAACCCTCGTGCCCTACGCGATGGTACTGGAAGCTGGTTCCTCACGCA
ATTGCCTTCTGTTTGAAGGAGTCGGGGAGCAAACCCCCCATGATCCGATACCGCAAAGAC
CGCGTTGTGGCGCGACGTGCCCCCTGCTTCCCGACGGTGACCAGCCTCCAGGACCTGGCC
AGTGGGGCCGCGCTGGCCGCCACAATCCACTGCTATTGTCCCCAGCTGCTGCGACTTGAG
GAGGTGTGCCTGAAGGACCCCATGTCTGTGGCGGACAGCCTGTACAACCTCCAGCTCGTG
CAGGATTTCTGTGCCTCTCGGCTGCCTCGTGGCTGCCCCCTGTCCCTTGAGGACCTGCTG
TACGTCCCACCGCCACTCAAGGTTAACCTGGTGGTGATGCTGGCCGAGTTGTTCATGTGT
TTTGAGGTGCTGAAGCCCGACTTTGTTCAAGTGAAGGACTTGCCCGATGGTCACGCTGCC
TCCCCCCGGGGCACTGAGGCCTCCCCACCTCAGAACAACAGCGGAAGTAGTTCTCCTGTC
TTCACCTTCCGCCACCCGCTTCTGTCATCTGGTGGCCCCCAGTCCCCACTCCGAGGATCC
ACCGGCTCCCTGAAGTCCTCCCCGTCCATGTCCCATATGGAGGCCCTGGGCAAGGCCTGG
AACCGGCAGCTCAGCCGTCCCCTCTCCCAGGCTGTGTCATTCAGCACCCCCTTTGGCCTG
GACAGCGACGTGGATGTCGTCATGGGCGACCCTGTGCTCCTCCGGTCTGTGAGTTCTGAC
AGCCTGGGCCCTCCGCGACCTGCGCCGGCCAGGACCCCTACCCAGCCACCCCCGGAGCCT
GGTGACCTGCCTACCATCGAGGAAGCTCTCCAGATCATCCACAGTGCTGAGCCCCGGCTC
CTCCCAGATGGGGCGGCCGACGGCAGCTTCTACCTCCACTCTCCTGAGGGGCCCTCCAAG
CCATCCCTGGCCTCCCCCTACCTGCCCGAGGGGACCTCCAAACCACTGTCCGACAGGCCT
ACCAAAGCACCAGTGTACATGCCACACCCCGAAACCCCCTCGAAACCATCTCCCTGTCTG
GTGGGGGAGGCATCGAAACCGCCAGCCCCCTCCGAGGGGTCCCCTAAGGCGGTGGCTTCG
TCCCCTGCAGCCACCAACTCTGAGGTGAAAATGACCAGCTTTGCAGAACGCAAGAAACAG
CTGGTGAAGGCAGAGGCTGAGGCCGGAGCGGGGTCCCCCACGTCCACTCCGGCCCCGCCG
GAGGCCCTGAGCTCGGAGATGAGTGAGCTCAGTGCTCGGCTGGAGGAGAAACGCAGAGCC
ATCGAGGCTCAGAAGCGACGGATTGAGGCCATATTCGCCAAGCACCGCCAGCGGCTCGGC
AAAAGCGCCTTCCTCCAGGTGCAGCCGCGGGAAGCCTCTGGGGAGGCGGAAGCAGAGGCG
GAGGAAGCCGATTCCGGTCCAGTCCCTGGAGGGGAGAGGCCCGCAGGCGAGGGCCAGGGA
GAGCCAACCTCACGGCCCAAGGCAGTGACCTTCTCGCCTGACCTGGGCCCGGTGCCTCAC
GAGGGGCTCGGGGAATACAATAGAGCGGTCAGCAAGCTGTCCGCCGCCTTGAGCTCGCTG
CAGCGGGACATGCAGAGACTTACGGACCAGCAGCAGCGGCTTCTGGCCCCGCCCGAGGCC
CCCGGATCTGCCCCACCACCAGCTGCGTGGGTCATCCCTGGCCCCACGACAGGACCCAAA
GCTGCATCTCCTAGCCCTGCCAGGCGAGTCCCTGCCACCCGGCGCAGCCCTGGGCCCGGG
CCCAGTCAGTCACCCAGAAGCCCGAAACACACGCGGCCAGCTGAGCTTCGGCTGGCACCC
TTGACCAGGGTGCTTACGCCACCCCACGACGTGGACAGTCTCCCCCACCTGAGAAAATTC
TCGCCGAGCCAGGTGCCCGTGCAGACACGCTCTTCCATCCTCCTGGCGGAGGAGACGCCC
CCTGAGGAGCCAGCTGCCCGGCCGGGACTCATCGAGATTCCGCTGGGCAGCCTGGCAGAT
CCCGCTGCCGAGGACGAGGGAGACGGGAGCCCTGCTGGCGCTGAGGATTCCTTGGAGGAG
GAGGCGTCTTCGGAGGGGGAGCCCAGGGTGGGACTGGGGTTCTTCTACAAGGATGAAGAC
AAGCCTGAGGACGAGATGGCCCAAAAGAGGGCCAGCCTGCTGGAGCGGCAGCAGAGGCGA
GCAGAGGAGGCGCGGCGCCGCAAGCAGTGGCAGGAGGTGGAGAAGGAACAGAGGAGGGAG
GAGGCCGCGAGGCTTGCTCAAGAGGAGGCCCCGGGCCCCGCCCCTTTAGTGTCCGCGGTC
CCTATGGCGACTCCAGCCCCTGCTGCCAGGGCTCCAGCTGAGGAGGAAGTGGGCCCCAGG
AAGGGGGACTTCACGCGGCAGGAGTACGAGCGGCGGGCCCAGCTGAAGCTGATGGACGAC
CTCGATAAGGTGCTGCGGCCCCGGGCTGCGGGGTCAGGAGGTCCAGGTAGGGGAGGGCGG
AGGGCCACACGGCCTCGCTCGGGTTGCTGTGATGACTCAGCCCTGGCACGAAGCCCAGCC
CGCGGCCTGCTGGGCTCTCGGCTGAGCAAAATCTATTCCCAGTCCACCCTCTCACTGTCC
ACAGTGGCCAACGAGGCCCACAATAACCTCGGGGTGAAGAGGCCTACGTCTCGGGCTCCT
TCCCCGTCAGGTCTGATGTCCCCAAGCCGGCTGCCTGGAAGCCGGGAACGGGACTGGGAA
AATGGCAGCAATGCCTCCTCCCCAGCGTCAGTGCCCGAGTACACCGGTCCACGGCTGTAC
AAAGAACCCAGCGCCAAGTCCAACAAGTTCATCATCCACAATGCCCTATCACACTGCTGC
CTGGCGGGAAAGGTGAACGAACCGCAGAAGAATCGCATTCTGGAGGAAATTGAGAAAAGC
AAGGCCAACCACTTCCTGATCCTCTTTCGCGACTCGAGCTGCCAGTTCCGCGCGCTCTAC
ACGCTGTCGGGGGAGACTGAGGAGCTGTCGCGGCTGGCAGGGTATGGGCCCCGGACCGTC
ACGCCCGCCATGGTGGAAGGCATCTACAAGTACAACTCGGACCGGAAGCGCTTCACCCAG
ATCCCCGCCAAGACCATGTCCATGAGTGTCGATGCCTTCACCATCCAGGGACACCTCTGG
CAGGGCAAGAAACCCACCACTCCCAAGAAGGGCGGCGGCACCCCCAAA
Protein sequence predicted based on nucleotide sequence
MVEAAPPGPGPLRRTFLVPEIKSLDQYDFSRAKAAASLAWVLRAAFGGAEHVPPELWEPF YTDQYAQEHVKPPVTRLLLSAELYCRAWRQALPQLETPPNPSALLALLARRGTVPALPER PVREADLRHQPILMGAHLAVIDALMAAFAFEWTKTLPGPLALTSLEHKLLFWVDTTVRRL QEKTEQEAAQRASPAAPADGAAPAQPSCPTRWYWKLVPHAIAFCLKESGSKPPMIRYRKD RVVARRAPCFPTVTSLQDLASGAALAATIHCYCPQLLRLEEVCLKDPMSVADSLYNLQLV QDFCASRLPRGCPLSLEDLLYVPPPLKVNLVVMLAELFMCFEVLKPDFVQVKDLPDGHAA SPRGTEASPPQNNSGSSSPVFTFRHPLLSSGGPQSPLRGSTGSLKSSPSMSHMEALGKAW NRQLSRPLSQAVSFSTPFGLDSDVDVVMGDPVLLRSVSSDSLGPPRPAPARTPTQPPPEP GDLPTIEEALQIIHSAEPRLLPDGAADGSFYLHSPEGPSKPSLASPYLPEGTSKPLSDRP TKAPVYMPHPETPSKPSPCLVGEASKPPAPSEGSPKAVASSPAATNSEVKMTSFAERKKQ LVKAEAEAGAGSPTSTPAPPEALSSEMSELSARLEEKRRAIEAQKRRIEAIFAKHRQRLG KSAFLQVQPREASGEAEAEAEEADSGPVPGGERPAGEGQGEPTSRPKAVTFSPDLGPVPH EGLGEYNRAVSKLSAALSSLQRDMQRLTDQQQRLLAPPEAPGSAPPPAAWVIPGPTTGPK AASPSPARRVPATRRSPGPGPSQSPRSPKHTRPAELRLAPLTRVLTPPHDVDSLPHLRKF SPSQVPVQTRSSILLAEETPPEEPAARPGLIEIPLGSLADPAAEDEGDGSPAGAEDSLEE EASSEGEPRVGLGFFYKDEDKPEDEMAQKRASLLERQQRRAEEARRRKQWQEVEKEQRRE EAARLAQEEAPGPAPLVSAVPMATPAPAARAPAEEEVGPRKGDFTRQEYERRAQLKLMDD LDKVLRPRAAGSGGPGRGGRRATRPRSGCCDDSALARSPARGLLGSRLSKIYSQSTLSLS TVANEAHNNLGVKRPTSRAPSPSGLMSPSRLPGSRERDWENGSNASSPASVPEYTGPRLY KEPSAKSNKFIIHNALSHCCLAGKVNEPQKNRILEEIEKSKANHFLILFRDSSCQFRALY TLSGETEELSRLAGYGPRTVTPAMVEGIYKYNSDRKRFTQIPAKTMSMSVDAFTIQGHLW QGKKPTTPKKGGGTPK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
