Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840092
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4569nts
Open reading frame: 1 to 4569
ATGGCGGCTGTGGGGTCAGGAGGCTATGCGCGGAACGATGCAGGGGAGAAGCTTCCCTCC
GTCATGGCGGGAGTTCCGGCGAGGAGGGGCCAGTCCTCACCGCCACCCGCTCCACCAATC
TGCCTGCGGAGGAGAACGCGACTTTCTACGGCCTCCGAGGAGACGGTGCAGAACCGGGTG
TCACTCGAGAAGGTGCTTGGCATCACAGCCCAGAACAGCAGTGGCCTAACCTGTGACCCC
GGAACAGGCCATGTGGCCTACCTGGCAGGATGTGTGGTGGTGATTTTGGACCCCAAGGAG
AACAAGCAGCAGCACATCTTTAATACCGCCAGGAAGTCTCTCAGTGCTCTGGCCTTCTCC
CCTGATGGCAAGTACATAGTGACAGGGGAGAATGGGCATAGGCCTGCTGTGCGCATCTGG
GATGTGGAGGAGAAGAATCAGGTGGCGGAGATGCTAGGCCACAAGTATGGTGTGGCGTGT
GTGGCCTTCTCACCCAATATGAAGCACATCGTGTCCATGGGCTACCAACATGACATGGTG
CTGAACGTCTGGGACTGGAAGAAAGACATCGTAGTGGCCTCCAACAAGGTATCTTGTAGA
GTCATTGCCCTCTCCTTCTCAGAGGACAGCAGCTATTTTGTCACTGTTGGCAACCGCCAT
GTGAGGTTCTGGTTCTTGGAAGTCTCCACTGAGACAAAGGTGACGTCTACAGTGCCCCTT
GTAGGGCGCTCGGGCATCCTGGGCGAGCTGCACAACAACATCTTCTGTGGTGTCGCCTGC
GGTCGGGGCAGGATGGCCGGCAGTACCTTCTGTGTGTCCTACTCGGGCCTCCTCTGCCAG
TTCAATGAGAAGAGGGTGCTGGAGAAGTGGATCAACCTGAAGGTGTCCCTGTCTTCTTGC
CTCTGTGTCAGCCAGGAGCTCATCTTCTGTGGCTGCACAGATGGGATAGTCAGGATCTTC
CAGGCCCATAGCCTCCACTACCTCGCCAACCTGCCCAAGCCACACTACCTTGGGGTAGAC
GTGGCACAGGGGCTGGAGCCCAGCTTCCTGTTCCACAGGAAGGCGGAAGCAGTCTACCCT
GATACAGTGGCACTGACCTTCGACCCCATCCACCAGTGGCTGTCCTGCGTGTATAAGGAC
CACAGCATCTACATCTGGGATGTCAAGGACATCAACAGAGTGGGCAAGGTGTGGTCAGAG
CTGTTCCACAGCTCCTACGTTTGGAACGTGGAGGTGTATCCTGAGTTTGAAGACCAGAGA
GCTTGTTTGCCATCAGGATCCTTTCTGACTTGTTCTTCAGACAACACCATTCGATTCTGG
AACTTGGACAGCAGCCCTGATTCCCACTGGCAGAAAAACATCTTCAGCAATACCCTGCTG
AAGGTCGTGTACGTCGAGAATGACATCCAGCATCTGCAGGACATGTCACACTTCCCAGAC
CGGGGGAGCGAGAATGGGACACCCATGGACGTGAAAGCCGGGGTCCGGGTCATGCAGGTC
AGTCCTGACGGCCAGCATTTGGCTTCAGGCGACCGAAGTGGAAATCTGAGGATCCACGAG
CTGCACTTCATGGACGAGCTGGTCAAGGTGGAGGCCCATGATGCCGAGGTGCTGTGCCTG
GAGTACTCCAAGCCAGAGACGGGGCTGACCTTGCTGGCCTCAGCCAGTCGCGACAGGCTG
ATCCATGTGCTCAACGTGGAGAAGAACTACAATCTGGAGCAGACGCTGGATGACCACTCC
TCCTCTATCACCGCCATCAAGTTCGCTGGCAACAGAGACATCCAGATGATCAGCTGTGGG
GCTGACAAGAGCATCTACTTTCGGTCTGCCCAGCAGGGTTCGGATGGACTACACTTTGTC
CGTACCCACCACGTAGCAGAGAAAACCACCTTGTATGACATGGACATCGACATCACCCAG
AAGTACGTGGCCGTGGCCTGCCAGGACCGCAATGTGAGAGTCTACAACACTGTGAACGGG
AAGCAGAAGAAGTGCTACAAGGGCTCCCAGGGTGACGAAGGGTCCTTGCTGAAGGTGCAT
GTGGACCCCTCAGGCACCTTCCTGGCCACCAGCTGCTCCGACAAAAGCATCTCAGTGATT
GACTTTTACTCGGGCGAGTGCATTGCCAAGATGTTTGGCCATTCAGAAATTATTACCTCA
ATGAAGTTCACCTATGACTGTCATCACCTGATCACAGTATCTGGAGACAGCTGCGTGTTC
ATCTGGCACCTGGGCCCGGAGATCACCAACTGCATGAAGCAGCACTTGCTGGAGATTGAC
CACCGGCAGCAGCAGCAGCACACAAATGACAAGAAGCGGAGTGGCCACCCCAGGCAGGAT
ACCTATGTGTCCACACCTAGTGAGATTCACTCCCTGAGCCCTGGAGAGCAAACTGAGGAT
GATCTGGAGGAAGAGTGTGAGCCAGAAGAGATGCTGAAGACACCCTCCAAAGATAGCTTG
GATCCAGATCCTCGTTGCCTGCTAACCAACGGCAAGCTGCCACTGTGGGCAAAGCGGCTG
CTGGGGGACGATGATGTGGCAGATGGCTTGGCCTTCCACGCCAAGCGCAGCTACCAGCCC
CACGGCCGATGGGCAGAGCGAGCCGGCCAAGAGCCCCTGAAGACCATCTTGGATGCCCAG
GACCTCGATTGCTACTTTACACCCATGAAGCCCGAGAGTCTGGAGAACTCCATTCTGGAT
TCACTGGAGCCACAGAGCCTGGCCAGCCTGCTGAGTGAGTCAGAGAGTCCCCAGGAAGCT
GGCCGCGGGCACCCCTCCTTCCTGCCTCAGCAGAAGGAATCATCTGAAGCCAGTGAGCTC
ATCCTCTACTCTCTGGAGGCCGAAGTGACAGTCACAGGGACAGACAGCCAGTATTGCAGG
AAGGAGGTGGAGGCCGGGCCTGGAGATCAGCAGGGCGACTCCTACCTCAGGGTGTCCTCC
GACAGCCCAAAGGACCAGAGTCCGCCTGAGGACTCGGGGGAGTCAGAGGCCGACCTGGAG
TGCAGCTTCGCAGCCATCCACTCCCCAGCTCCGCCTCCTGACCCAGCCCCTAGGTTTGCC
ACGTCGCTGCCCCATTTCCCAGGATGCGCAGGTCCCACAGAAGATGAGCTGTCCCTGCCC
GAGGGACCCAGCGTCCCCAGCAGCTCCCTGCCCCAGACTCCGGAGCAGGAGAAGTTCCTC
CGCCACCACTTTGAGACACTGACTGAGTCCCCCTGCAGAGAGCTCTTCCCCGCAGCTCTT
GGAGACGTGGAGGCCTCTGAAGCTGAAGACCACTTCTTCAACCCACGCCTGAGTATCTCC
ACGCAGTTCCTCTCAAGCCTCCAGAAGGCATCCAGGTTCACCCATACCTTCCCTCCCCGG
GCAACCCAGTGCCTTGTGAAGTCTCCTGAGGTCAAGCTGATGGACCGAGGCGGAAGCCAG
CCCAGAGCAGGTACTGGCTACGCCTCCCCAGACAGGACCCACGTCCTCGCTGCAGGGAAG
GCTGAAGAAACCCTGGAGGCCTGGCGACCACCACCTCCCTGCCTTACGAGCCTGGCGTCC
TGTGTCCCTGCTTCTTCCGTGCTGCCCACAGACAGGAATCTCCCAACGCCCACATCTGCA
CCCACCCCTGGACTGGCTCAGGGCGTCCATGCCCCCTCCACCTGTTCCTACATGGAGGCC
ACTGCCAGCTCCCGGGCCAGGATATCACGCAGCATCTCCCTCGGTGACAGTGAGGGCCCT
ATCGTGGCCACACTGGCCCAGCCCCTTCGTAGGCCATCTTCTGTTGGGGAGCTGGCCTCC
CTGGGCCAGGAGCTTCAGGCCATCACCACCGCGACAACACCCAGTTTGGACAGCGAGGGC
CAAGAGCCTGCCCTGCGTTCTTGGGGCAACCACGAGGCCAGGGCCAACCTGAGACTGACC
CTCTCAAGTGCCTGTGATGGGCTCCTGCAGCCCCCCGTGGATACCCAGCCTGGCGTTACC
GTCCCTGCAGTCTCCTTCCCAGCCCCTAGCCCTGTGGAAGAGTCTGCCCTGCGGCTCCAC
GGCTCTGCCTTTCGCCCAAGTCTCCCAGCTCCTGAGTCTCCTGGCCTTCCTGCCCACCCC
AGTAACCCCCAGCTTCCAGAGGCTCGGCCTGGCATCCCTGGCGGCACTGCTTCCCTCCTG
GAGCCCACCTCTGGTGCACTTGGTCTGTTACAGGGCAGCCCTGCCCGCTGGAGTGAGCCC
TGGGTGCCGGTTGAAGCCCTGCCCCCATCTCCCCTTGAGCTCAGCAGGGTGGGGAACATC
TTGCACAGGCTGCAGACCACCTTCCAAGAAGCCCTGGACCTTTACCGTGTGTTGGTGTCC
AGCGGCCAGGTGGACACCGGGCAGCAGCAGGCACGGACTGAGCTGGTGTCTACCTTCCTG
TGGATCCACAGCCAGCTGGAGGCTGAATGCCTGGTGGGGACTAGTGTGGCCCCAGCCCAA
GCCCTGCCCAGCCCAGGACCCCCGAGTCCACCGACGCTTTACCCTCTTGCCAGCCCAGAC
CTGCAAGCCCTCCTGGAACACTACTCGGAACTGCTGGTGCAGGCCGTGCGGAGGAAGGCA
CGGGGGCAC
Protein sequence predicted based on nucleotide sequence
MAAVGSGGYARNDAGEKLPSVMAGVPARRGQSSPPPAPPICLRRRTRLSTASEETVQNRV SLEKVLGITAQNSSGLTCDPGTGHVAYLAGCVVVILDPKENKQQHIFNTARKSLSALAFS PDGKYIVTGENGHRPAVRIWDVEEKNQVAEMLGHKYGVACVAFSPNMKHIVSMGYQHDMV LNVWDWKKDIVVASNKVSCRVIALSFSEDSSYFVTVGNRHVRFWFLEVSTETKVTSTVPL VGRSGILGELHNNIFCGVACGRGRMAGSTFCVSYSGLLCQFNEKRVLEKWINLKVSLSSC LCVSQELIFCGCTDGIVRIFQAHSLHYLANLPKPHYLGVDVAQGLEPSFLFHRKAEAVYP DTVALTFDPIHQWLSCVYKDHSIYIWDVKDINRVGKVWSELFHSSYVWNVEVYPEFEDQR ACLPSGSFLTCSSDNTIRFWNLDSSPDSHWQKNIFSNTLLKVVYVENDIQHLQDMSHFPD RGSENGTPMDVKAGVRVMQVSPDGQHLASGDRSGNLRIHELHFMDELVKVEAHDAEVLCL EYSKPETGLTLLASASRDRLIHVLNVEKNYNLEQTLDDHSSSITAIKFAGNRDIQMISCG ADKSIYFRSAQQGSDGLHFVRTHHVAEKTTLYDMDIDITQKYVAVACQDRNVRVYNTVNG KQKKCYKGSQGDEGSLLKVHVDPSGTFLATSCSDKSISVIDFYSGECIAKMFGHSEIITS MKFTYDCHHLITVSGDSCVFIWHLGPEITNCMKQHLLEIDHRQQQQHTNDKKRSGHPRQD TYVSTPSEIHSLSPGEQTEDDLEEECEPEEMLKTPSKDSLDPDPRCLLTNGKLPLWAKRL LGDDDVADGLAFHAKRSYQPHGRWAERAGQEPLKTILDAQDLDCYFTPMKPESLENSILD SLEPQSLASLLSESESPQEAGRGHPSFLPQQKESSEASELILYSLEAEVTVTGTDSQYCR KEVEAGPGDQQGDSYLRVSSDSPKDQSPPEDSGESEADLECSFAAIHSPAPPPDPAPRFA TSLPHFPGCAGPTEDELSLPEGPSVPSSSLPQTPEQEKFLRHHFETLTESPCRELFPAAL GDVEASEAEDHFFNPRLSISTQFLSSLQKASRFTHTFPPRATQCLVKSPEVKLMDRGGSQ PRAGTGYASPDRTHVLAAGKAEETLEAWRPPPPCLTSLASCVPASSVLPTDRNLPTPTSA PTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELAS LGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLQPPVDTQPGVT VPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGGTASLL EPTSGALGLLQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVS SGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPD LQALLEHYSELLVQAVRRKARGH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
