Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840098
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5499nts
Open reading frame: 1 to 5499
ATGAGAGAGAAGGAGCAAGAAAGGGAAGAACAGTTAATGGAAGACAAGAAAAGGAAGAAA
GAGGATAAAAAGAAAAAAGAAGCCACTCAGAAGGTCACGGAACAAAAAACCAAAGTGCCT
GAAGTGACGAAACCAAGTTTAAGCCAACCAACGGCCGCCAGCCCAATTGGCAGCTCTCCA
TCGCCACCAGTCAATGGTGGAAACAATGCCAAAAGGGTGGCAGTGCCGAACGGACAACCG
CCAAGCGCCGCCCGCTACATGCCTCGGGAGGTGCCGCCGCGATTCCGTTGCCAGCAGGAC
CACAAAGTGTTACTAAAACGTGGGCAGCCCCCTCCTCCGTCTTGCATGCTCCTTGGGGGT
GGGGCAGGGCCTCCTCCCTGCACAGCACCTGGAGCAAACCCCAACAACGCACAAGTGACA
GGAGCGCTGTTGCAGAGCGAGAGTGGGACTGCGCCAGACTCTACCCTTGGAGGTGCTGCT
GCTTCAAATTATGCAAATTCCACTTGGGGCTCGGGAGCCTCCTCCAACAACGGCACCTCC
CCTAACCCAATTCACATCTGGGACAAGGTGATTGTAGACGGGTCTGACATGGAAGAGTGG
CCTTGTATTGCCAGCAAAGACACTGAATCTTCTTCCGAAAACACCACCGATAACAACAGT
GCCTCGAACCCTGGCTCTGAGAAGAGCACTCTGCCAGGCAGCACCACTAGTAACAAAGGA
AAAGGGAGCCAGTGCCAGTCTGCAAGTTCTGGGAACGAATGTAATCTTGGGGTCTGGAAA
TCTGACCCTAAGGCTAAATCTGTTCAATCTTCCAACTCTACTACAGAGAACAACAATGGA
CTAGGAAATTGGAGGAATGTGAGTGGTCAGGATAGAATCGGACCTGGCTCTGGCTTCAGC
AACTTTAACCCAAATTCCAATCCATCTGCCTGGCCAGCACTTGTCCAAGAAGGAACTTCT
AGGAAAGGGGCATTGGAAACAGATAATAGTAATTCCAGTGCACAGGTTAGCACAGTAGGT
CAGACATCTAGGGAACAGCAGTCAAAGATGGAAAATGCGGGTGTTAATTTTGTTGTGTCT
GGCAGAGAACAGGCTCAAATTCATAACACTGATGGACCAAAAAATGGAAACACTAACTCA
TTGAACCTCAGTTCACCAAACCCCATGGAGAATAAGGGAATGCCCTTTGGAATGGGCTTG
GGGAACACCTCCAGGAGCACTGATGCCCCTTCCCAAAGCACTGGAGATAGAAAGACTGGG
AGTGTTGGATCTTGGGGTGCAGCTAGGGGGCCTTCTGGAACTGACACAGTCTCTGGACAA
AGCAATTCTGGAAACAATGGGAACAATGGAAAAGAGAGGGAGGACTCCTGGAAAGGAGCT
TCTGTTCAGAAATCAACTGGGTCAAAAAATGACTCTTGGGACAACAATAACAGGTCTACG
GGTGGGTCCTGGAACTTTGGCCCCCAGGACTCTAATGACAACAAATGGGGTGAAGGGAAC
AAAATGACATCTGGGGTCAGTCAGGGAGAATGGAAACAGCCGACTGGGTCTGATGAGTTG
AAAATTGGAGAATGGAGTGGTCCAAACCAACCAAATTCTAGCACAGGAGCATGGGACAAT
CAAAAGGGCCACCCCCTCCCCGAAAACCAAGGCAATGCCCAGGCTCCCTGTTGGGGAAGA
TCTTCCAGCTCCACCGGAAGTGAAGTTGGAGGTCAAAGCACTGGAAGCAACCACAAAGCA
GGAAGTAGTGACAGTCATAACTCAGGCCGCCGGTCGTACAGGCCTACACATCCTGATTGT
CAGGCTGTCTTGCAGACTCTTTTGAGCCGAACTGATTTGGACCCCAGGGTGCTGTCAAAC
ACTGGCTGGGGCCAAACTCAAATTAAGCAGGACACAGTGTGGGACATTGAAGAGGTGCCA
AGACCTGAGGGGAAATCTGACAAAGGAACTGAGGGGTGGGAGAGCGCTGCCACACAGACC
AAGAACTCAGGGGGCTGGGGAGATGCACCCAGCCAAAGCAATCAAATGAAGTCTGGATGG
GGGGAGCTCTCAGCCTCTACAGAGTGGAAAGACCCCAAGAACACAGGAGGCTGGAATGAC
TACAAGAACAACAACTCTTCCAACTGGGGAGGAGGACGACCTGATGAAAAGACACCTTCA
TCTTGGAATGAGAATCCCAGCAAGGATCAGGGGTGGGGAGGCGGACGTCAGCCCAATCAA
GGATGGTCTTCAGGAAAGAATGGTTGGGGCGAGGAAGTCGATCAGACAAAAAACAGTAAT
TGGGAAAGTTCTGCCAGCAAACCTGTGTCTGGGTGGGGTGAAGGAGGGCAGAATGAAATC
GGGACTTGGGGTAATGGTGGCAATGCAAGCCTAGCTTCAAAAGGTGGGTGGGAGGATTGC
AAAAGATCTCCAGCATGGAATGAGACGGGCCGACAGCCCAATTCTTGGAATAAACAACAC
CAACAGCAGCAGCCCCCACAGCAGCCGCCGCCACCACAACCAGAGGCTTCTGGTTCATGG
GGAGGCCCACCCCCACCACCTCCAGGCAACGTTCGACCTTCCAATTCCAGCTGGAGCAGC
GGGCCACAGCCTGCAACACCTAAGGATGAGGAACCCAGTGGTTGGGAAGAGCCATCCCCA
CAGTCAATTAGTCGGAAAATGGACATTGATGATGGCACTTCAGCATGGGGAGATCCTAAC
AGTTATAACTACAAGAATGTGAATCTGTGGGATAAGAATTCCCAAGGGGGCCCAGCACCT
CGAGAACCCAACCTGCCCACCCCCATGACCAGTAAATCGGCATCAGTCTGGAGCAAATCC
ACACCCCCTGCTCCAGATAATGGTACTTCCGCTTGGGGCGAGCCAAATGAAAGCAGTCCT
GGGTGGGGCGAGATGGATGATACAGGAGCATCGACCACAGGCTGGGGGAACACGCCCGCC
AACGCTCCCAATGCCATGAAGCCTAATTCCAAATCTATGCAAGACGGCTGGGGGGAGAGT
GACGGGCCAGTCACAGGAGCTCGCCATCCCAGCTGGGAAGAGGAGGAGGATGGCGGAGTC
TGGAACACCACTGGCTCTCAGGGCAGTGCATCCTCCCACAACTCCGCAAGCTGGGGACAA
GGAGGAAAGAAACAAATGAAGTGCTCACTCAAAGGAGGAAACAATGATTCATGGATGAAT
CCTCTCGCCAAACAGTTTTCCAATATGGGATTGCTGAGTCAGACTGAAGATAATCCAAGC
AGCAAAATGGATTTGTCTGTAGGAAGCCTTTCAGATAAAAAATTTGATGTGGACAAGCGA
GCGATGAACCTCGGGGATTTTAATGATATCATGAGGAAGGATCGATCTGGGTTCCGTCCA
CCTAACTCCAAAGACATGGGAACCACAGATAGTGGGCCTTATTTTGAGAAGCTGACTTTG
CCTTTCTCCAATCAAGATGGGTGCCTTGGGGATGAGGCTCCCTGCTCTCCCTTCTCCCCT
TCTCCCAGCTACAAGCTGTCTCCCTCTGGTTCCACACTACCCAACGTCAGCCTTGGAGCA
ATCGGCACAGGGCTGAACCCCCAAAACTTCGCTGCTAGGCAAGGCGGTAGTCATGGTTTG
TTTGGAAACAGCACAGCACAATCGAGGGGTCTGCACACACCCGTGCAGCCACTAAATTCT
TCTCCCAGTCTTCGGGCGCAAGTGCCTCCCCAGTTTATTTCCCCCCAGGTTTCTGCCTCA
ATGCTCAAGCAGTTTCCCAACAGTGGCCTCAGTCCAGGTCTTTTCAATGTGGGCCCCCAG
TTATCTCCTCAACAAATTGCCATGCTGAGCCAGCTTCCACAAATTCCCCAGTTTCAGTTG
GCATGTCAGCTTCTCTTGCAGCAGCAGCAACAGCAGCAGTTGTTACAGAACCAGAGAAAG
ATTTCTCAAGCTGTACGCCAACAGCAAGAGCAGCAGCTGGCTCGAATGGTGAGTGCACTC
CAGCAGCAGCAGCAGCAGCAGCAGAGACAGCCTGGCATGAAGCACTCGCCCTCTCATCCT
GTTGGGCCCAAGCCGCATCTGGACAACATGGTACCCAACGCATTGAATGTGGGGCTCCCA
GACCTTCAAACCAAAGGGCCAATACCTGGATATGGCTCTGGCTTCAGCTCTGGCGGCATG
GACTATGGCATGGTTGGTGGGAAGGAGGCTGGAACCGAGTCTCGCTTTAAACAGTGGACC
TCCATGATGGAGGGGCTGCCCTCTGTAGCCACACAGGAAGCCAATATGCACAAAAATGGC
GCTATAGTGGCCCCTGGTAAAACCCGGGGAGGGTCACCGTACAACCAGTTTGATATCATC
CCTGGTGACACACTGGGTGGCCATACGGGTCCTGCTGGTGATAGCTGGTTACCTGCCAAA
TCTCCACCAACAAATAAAATCGGAAGTAAATCCAGCAATGCCAGTTGGCCTCCCGAATTC
CAACCAGGAGTGCCATGGAAAGGTATCCAAAACATTGACCCTGAATCTGACCCCTATGTC
ACCCCAGGAAGTGTGCTGGGGGGTACAGCCACATCTCCCATTGTAGATACTGACCACCAA
CTGCTGCGGGATAACACCACAGGGTCTAATTCTTCTCTCAACACATCGCTGCCTTCACCT
GGCGCCTGGCCTTACAGTGCCTCTGACAACTCCTTTACAAACGTTCATAGCACTTCAGCA
AAGTTCCCTGATTACAAATCAACATGGTCCCCAGATCCCATAGGACACAACCCCACTCAT
CTCTCCAACAAGATGTGGAAAAACCATATTTCCTCCAGGAACACTACACCGCTGCCCCGC
CCACCTCCTGGTCTGACCAACCCCAAACCATCATCTCCCTGGAGCAGCACAGCACCCCGA
TCAGTCAGGGGGTGGGGGACACAGGACTCACGGCTCGCCTCGGCCTCTACCTGGAGTGAT
GGTGGCTCCGTTCGTCCTAGTTACTGGCTGGTTCTTCACAATCTCACCCCACAGATTGAT
GGGTCAACCTTGAGAACGATCTGCATGCAGCATGGCCCACTGCTGACATTCCATCTGAAT
CTAACCCAGGGGACTGCCCTGATCCGATACAGCACCAAACAGGAGGCGGCCAAGGCCCAA
ACTGCACTGCACATGTGTGTGTTGGGAAACACTACCATCCTTGCTGAGTTTGCCACTGAT
GATGAAGTCAGCCGCTTTCTGGCACAAGCTCAGCCTCCTACTCCTGCAGCAACCCCAAGT
GCGCCAGCAGCGGGGTGGCAGTCGCTGGAGACAGGCCAGAACCAGTCCGATCCCGTGGGA
CCTGCTCTGAATCTTTTTGGTGGGTCCACTGGGCTCGGGCAGTGGAGCAGCAGCGCTGGT
GGCAGCAGCGGGGCCGATCTTGCAGGCGCTTCCTTGTGGGGGCCCCCAAACTATTCTTCT
AGCTTATGGGGAGTCCCAACGGTGGAAGATCCCCATAGGATGGGCAGCCCTGCTCCTTTA
CTGCCTGGTGACCTTCTGGGAGGAGGGTCGGATTCAATC
Protein sequence predicted based on nucleotide sequence
MREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSP SPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGG GAGPPPCTAPGANPNNAQVTGALLQSESGTAPDSTLGGAAASNYANSTWGSGASSNNGTS PNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKG KGSQCQSASSGNECNLGVWKSDPKAKSVQSSNSTTENNNGLGNWRNVSGQDRIGPGSGFS NFNPNSNPSAWPALVQEGTSRKGALETDNSNSSAQVSTVGQTSREQQSKMENAGVNFVVS GREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNTSRSTDAPSQSTGDRKTG SVGSWGAARGPSGTDTVSGQSNSGNNGNNGKEREDSWKGASVQKSTGSKNDSWDNNNRST GGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDN QKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDC QAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQT KNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTPS SWNENPSKDQGWGGGRQPNQGWSSGKNGWGEEVDQTKNSNWESSASKPVSGWGEGGQNEI GTWGNGGNASLASKGGWEDCKRSPAWNETGRQPNSWNKQHQQQQPPQQPPPPQPEASGSW GGPPPPPPGNVRPSNSSWSSGPQPATPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPN SYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSP GWGEMDDTGASTTGWGNTPANAPNAMKPNSKSMQDGWGESDGPVTGARHPSWEEEEDGGV WNTTGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPS SKMDLSVGSLSDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTL PFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGSHGL FGNSTAQSRGLHTPVQPLNSSPSLRAQVPPQFISPQVSASMLKQFPNSGLSPGLFNVGPQ LSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKISQAVRQQQEQQLARMVSAL QQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGM DYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDII PGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYV TPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSA KFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPR SVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLN LTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPS APAAGWQSLETGQNQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSS SLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
