Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840313
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5208nts
Open reading frame: 1 to 5208
ATGGAGCGGTGCAGCCGATGCCATCGCCTCCTCCTGCTCCTACCTCTGGTGCTGGGGCTG
AGCGCGGCCCCAGGCTGGGCTGGTGCACCCCCTGTGGATGTCCTGCGGGCCCTGAGGTTC
CCCTCCCTCCCTGATGGTGTCAGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTAC
CGAGTGGCACGACCTGCGCAGCTCAGTGCACCCACTCGCCAGCTTTTCCCAGGAGGATTT
CCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCCCTGGTCTACAAGCTCCCCTC
CTCACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTTGAGCTGGGACGACCTGTC
CGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTCTTCCGA
GGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCCGTGGCTGTGAAGGGCCAGTCT
GTCACCCTCATTGTTGACTGCAAGAAGCGAGTCACCAGGCCACTCCCCCGAAGTGCTCGT
CCAGTATTGGACACCCATGGAGTGATCATCTTTGGTGCCCGTATTCTCGATGAAGAAGTC
TTTGAGGGTGATGTCCAGGAGCTGGCCATTGTCCCAGGGGTGCAGGCAGCCTATGAATCA
TGTGAACAGAAGGAGCTGGAATGCGAGGGTGGCCAGAGGGAAAGACCTCAAAACCAACAG
CCTCACAGAGCCCAGAGATCCCCTCAGCAGCAACCATCAAGACTGCACAGGCCTCAAAAT
CAGGAACCCCAGAGCCAGCCCACTGAGTCTCTCTACTATGACTACGAGCCCCCCTATTAT
GATGTGATGACTACGGGGACAACCCCTGATTATCAGGACCCCACCCCAGGTGAAGAGGAA
GAAATCCTGGAGTCGAGCCTCTTGCCACCCCTTGAGGAGGAGCAGACAGATCTGCAGGTC
CCCCCCACAGCCGACAGGTTTCAGGCAGAGGAATATGGGGAGGGTGGTACAGACCCCCCT
GAAGGCCCCTACGATTACACCTATGGCTATGGGGATGATTATCGTGAGGAGACAGAGCTT
GGCCCTGCCCTCTCTGCGGAGACAGCCCACTCAGGAGCCGCTGCCCATGGACCCCGAGGG
CTGAAGGGCGAAAAAGGAGAACCTGCAGTGTTGGAACCTGGTATGCTCGTGGAGGGGCCG
CCTGGCCCAGAAGGCCCTGCGGGATTGATTGGTCCCCCTGGCATCCAGGGGAACCCAGGC
CCAGTTGGAGATCCCGGAGAGCGGGGCCCCCCTGGCCGAGCAGGGCTTCCTGGATCAGAT
GGGGCTCCTGGTCCTCCTGGCACATCTCTCATGCTCCCATTCCGGTTTGGCAGTGGTGGG
GGTGACAAGGGACCTGTGGTGGCGGCCCAGGAGGCTCAGGCGCAGGCCATCCTGCAGCAA
GCGAGACTTGCGCTCCGTGGACCCCCTGGCCCCATGGGATACACAGGGCGCCCTGGGCCC
TTGGGCCAACCTGGGAGCCCCGGCTTGAAAGGCGAGTCTGGAGACTTAGGACCGCAGGGG
CCCAGAGGACCACAGGGCCTCACAGGCCCTCCCGGCAAGGCTGGGCGAAGGGGACGCGCA
GGAGCTGATGGAGCTCGAGGGATGCCCGGAGATCCTGGAGTGAAGGGTGACCGAGGTTTT
GATGGACTCCCCGGGCTCCCTGGCGAAAAGGGCCATAGGGGTGATACTGGTGCCCAAGGC
CTCCCTGGTCCACCCGGTGAGGATGGAGAGAGGGGAGATGACGGGGAGATTGGCCCTCGA
GGCCTGCCTGGAGAGTCTGGACCACGAGGCCTCCTTGGTCCCAAAGGCCCGCCTGGTATT
CCCGGACCCCCTGGCGTCCGAGGCATGGATGGTCCCCAGGGCCCAAAAGGGAGCTTGGGA
CCCCAGGGTGAGCCCGGACCGCCTGGACAACAGGGAACCCCCGGGACCCAGGGTCTTCCC
GGGCCCCAGGGTGCCATCGGCCCTCATGGAGAGAAGGGTCCTCAAGGCAAGCCAGGGCTC
CCCGGCATGCCTGGCTCAGACGGGCCTCCGGGTCACCCCGGGAAGGAAGGTCCCCCTGGA
ACCAAAGGAAACCAGGGTCCCTCTGGACCTCAGGGACCTCTAGGATACCCCGGCCCTCGC
GGCGTCAAGGGCGTGGACGGAATTCGGGGTCTGAAGGGTCATAAGGGTGAGAAAGGGGAG
GATGGCTTCCCTGGGTTCAAAGGCGACATAGGCGTGAAAGGTGACAGGGGCGAAGTTGGA
GTCCCCGGTTCAAGGGGAGAGGATGGTCCTGAGGGGCCAAAGGGACGCACTGGACCGACT
GGTGACCCAGGGCCCCCAGGGCTCATGGGCGAGAAAGGCAAGCTGGGTGTTCCTGGTCTG
CCTGGCTATCCTGGACGTCAGGGACCCAAGGGGTCCCTAGGATTTCCTGGCTTTCCTGGT
GCTAGTGGAGAGAAGGGCGCCCGGGGCCTGTCAGGGAAGTCGGGTCCTCGGGGAGAACGG
GGCCCTACCGGTCCACGAGGTCAGCGGGGACCCCGAGGTGCCACTGGGAAGTCTGGAGCT
AAGGGAACATCTGGTGGTGATGGCCCCCATGGGCCCCCCGGAGAGAGGGGCCTACCTGGA
CCTCAAGGTCCTAACGGGTTTCCTGGACCAAAAGGACCACCCGGCCCCCCTGGGAAGGAT
GGGCTGCCCGGACACCCAGGCCAACGAGGAGAAGTGGGTTTCCAAGGGAAGACCGGCCCA
CCTGGTCCTCCAGGAGTGGTGGGACCTCAGGGAGCAGCCGGAGAAACCGGACCTATGGGG
GAGAGAGGTCACCCAGGACCACCGGGGCCCCCTGGAGAGCAGGGACTGCCTGGGACAGCT
GGAAAAGAAGGAACAAAGGGCGACCCCGGGCCACCTGGGGCCCCAGGAAAGGATGGACCC
GCTGGTCTGCGGGGATTCCCAGGAGAGAGAGGCCTTCCAGGCACTGCTGGTGGACCTGGT
TTGAAGGGGAATGAAGGTCCGTCTGGCCCCCCTGGCCCCGCAGGCTCACCTGGGGAACGA
GGTGCAGCAGGATCAGGGGGACCCATTGGTCCGCCAGGGCGACCAGGCCCTCAGGGTCCA
CCTGGCGCAGCAGGAGAGAAAGGTGTCCCAGGTGAGAAGGGCCCTATTGGCCCGACTGGC
CGAGATGGAGTGCAGGGTCCTGTCGGGCTACCCGGTCCTGCTGGACCTCCCGGTGTGGCT
GGAGAGGACGGAGACAAGGGTGAGGTGGGGGACCCCGGACAGAAGGGCACCAAGGGGAAC
AAGGGTGAACATGGCCCTCCTGGACCCCCTGGACCCATCGGTCCTGTTGGGCAGCCTGGA
GCAGCGGGAGCAGATGGGGAGCCCGGAGCTAGGGGGCCACAGGGACACTTTGGAGCCAAA
GGTGATGAAGGAACAAGAGGATTCAATGGGCCTCCAGGTCCCATTGGCCTACAGGGTTTG
CCAGGCCCCTCTGGCGAGAAGGGAGAAACAGGAGATGTGGGTCCTATGGGACCACCTGGC
CCCCCAGGACCTCGAGGACCAGCCGGACCCAATGGCGCTGATGGCCCACAAGGACCCCCA
GGAGGTGTTGGGAACCTGGGTCCGCCTGGAGAGAAGGGGGAGCCAGGGGAGTCAGGATCT
CCAGGTATCCAGGGCGAGCCAGGTGTCAAGGGTCCAAGAGGGGAACGTGGGGAGAAAGGA
GAGTCGGGGCAGCCAGGAGAGCCTGGGCCACCAGGGCCTAAAGGCCCCACAGGCGATGAT
GGCCCTAAAGGGAACCCTGGACCCGTTGGTTTTCCTGGCGACCCTGGCCCCCCTGGAGAA
GGTGGGCCTCGGGGCCAGGATGGTGCTAAGGGGGACCGAGGCGAGGATGGTGAGCCAGGA
CAGCCTGGATCCCCTGGGCCCACCGGGGAGAATGGACCCCCAGGGCCACTTGGAAAGCGA
GGTCCTGCCGGCTCCCCTGGTTCCGAGGGGCGACAAGGAGGGAAGGGAGCCAAGGGCGAT
CCTGGCGCTATAGGTGCCCCGGGCAAGACCGGACCGGTGGGTCCTGCAGGCCCAGCAGGG
AAACCTGGCCCTGATGGTCTGAGGGGGCTGCCAGGCTCAGTGGGTCAGCAGGGCCGACCT
GGAGCTACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTGCCTGGTCTT
CGGGGCGATGCTGGCGCTAAGGGAGAGAAGGGCCACCCCGGTCTGATTGGACTGATTGGG
CCCCCGGGTGAGCAGGGAGAAAAGGGAGATCGGGGACTTCCTGGTCCTCAGGGCTCTCCC
GGGCAGAAGGGTGAGATGGGTATCCCAGGAGCATCCGGCCCCATTGGGCCTGGAGGTCCC
CCCGGCCTCCCCGGACCTGCTGGCCCCAAAGGTGCCAAAGGAGCCACAGGACCAGGAGGC
CCCAAGGGAGAGAAGGGTGTGCAGGGCCCTCCGGGACACCCGGGTCCCCCAGGCGAGGTG
ATCCAGCCACTGCCCATTCAGATGCCCAAGAAGACTCGGCGCTCGGTGGATGGAAGCCGT
CTGATGCAGGAAGATGAGGCCATACCGACCGGGGGAGCCCCCGGCAGTCCTGGCGGGCTG
GAGGAGATCTTTGGCTCACTCGACTCCCTGCGGGAGGAGATCGAGCAGATGAGGCGGCCA
ACAGGGACTCAGGACAGTCCTGCTCGAACCTGCCAGGACCTGAAGCTGTGCCACCCAGAG
CTTCCCGATGGCGAGTACTGGGTCGACCCCAACCAGGGATGTGCTCGGGATGCCTTCCGA
GTTTTCTGCAACTTCACAGCAGGGGGTGAGACATGTGTGACGCCTAGGGATGACGTCACG
CAGTTCTCTTACGTGGACTCAGAGGGCTCCCCAGTGGGTGTGGTCCAGCTCACCTTCCTG
CGGCTGCTGAGTGTCTCAGCCCACCAGGACGTCTCCTACCCCTGCTCCGGCGCAGCCCGT
GACGGTCCCCTGAGACTCCGTGGGGCCAATGAGGATGAGCTGAGCCCCGAGACTAGCCCC
TATGTCAAAGAATTCAGAGATGGCTGCCAGACACAGCAAGGCCGGACGGTGCTGGAGGTG
CGAACGCCTGTGCTGGAGCAGCTGCCAGTGCTGGATGCCTCTTTCTCAGACCTGGGCGCC
CCACCGAGGCGGGGAGGGGTGCTGCTGGGGCCTGTCTGCTTCATGGGA
Protein sequence predicted based on nucleotide sequence
MERCSRCHRLLLLLPLVLGLSAAPGWAGAPPVDVLRALRFPSLPDGVRRAKGICPADVAY RVARPAQLSAPTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVRQLGLELGRPV RFLYEDQTGRPQPPSQPVFRGLSLADGKWHRVAVAVKGQSVTLIVDCKKRVTRPLPRSAR PVLDTHGVIIFGARILDEEVFEGDVQELAIVPGVQAAYESCEQKELECEGGQRERPQNQQ PHRAQRSPQQQPSRLHRPQNQEPQSQPTESLYYDYEPPYYDVMTTGTTPDYQDPTPGEEE EILESSLLPPLEEEQTDLQVPPTADRFQAEEYGEGGTDPPEGPYDYTYGYGDDYREETEL GPALSAETAHSGAAAHGPRGLKGEKGEPAVLEPGMLVEGPPGPEGPAGLIGPPGIQGNPG PVGDPGERGPPGRAGLPGSDGAPGPPGTSLMLPFRFGSGGGDKGPVVAAQEAQAQAILQQ ARLALRGPPGPMGYTGRPGPLGQPGSPGLKGESGDLGPQGPRGPQGLTGPPGKAGRRGRA GADGARGMPGDPGVKGDRGFDGLPGLPGEKGHRGDTGAQGLPGPPGEDGERGDDGEIGPR GLPGESGPRGLLGPKGPPGIPGPPGVRGMDGPQGPKGSLGPQGEPGPPGQQGTPGTQGLP GPQGAIGPHGEKGPQGKPGLPGMPGSDGPPGHPGKEGPPGTKGNQGPSGPQGPLGYPGPR GVKGVDGIRGLKGHKGEKGEDGFPGFKGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPT GDPGPPGLMGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGASGEKGARGLSGKSGPRGER GPTGPRGQRGPRGATGKSGAKGTSGGDGPHGPPGERGLPGPQGPNGFPGPKGPPGPPGKD GLPGHPGQRGEVGFQGKTGPPGPPGVVGPQGAAGETGPMGERGHPGPPGPPGEQGLPGTA GKEGTKGDPGPPGAPGKDGPAGLRGFPGERGLPGTAGGPGLKGNEGPSGPPGPAGSPGER GAAGSGGPIGPPGRPGPQGPPGAAGEKGVPGEKGPIGPTGRDGVQGPVGLPGPAGPPGVA GEDGDKGEVGDPGQKGTKGNKGEHGPPGPPGPIGPVGQPGAAGADGEPGARGPQGHFGAK GDEGTRGFNGPPGPIGLQGLPGPSGEKGETGDVGPMGPPGPPGPRGPAGPNGADGPQGPP GGVGNLGPPGEKGEPGESGSPGIQGEPGVKGPRGERGEKGESGQPGEPGPPGPKGPTGDD GPKGNPGPVGFPGDPGPPGEGGPRGQDGAKGDRGEDGEPGQPGSPGPTGENGPPGPLGKR GPAGSPGSEGRQGGKGAKGDPGAIGAPGKTGPVGPAGPAGKPGPDGLRGLPGSVGQQGRP GATGQAGPPGPVGPPGLPGLRGDAGAKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSP GQKGEMGIPGASGPIGPGGPPGLPGPAGPKGAKGATGPGGPKGEKGVQGPPGHPGPPGEV IQPLPIQMPKKTRRSVDGSRLMQEDEAIPTGGAPGSPGGLEEIFGSLDSLREEIEQMRRP TGTQDSPARTCQDLKLCHPELPDGEYWVDPNQGCARDAFRVFCNFTAGGETCVTPRDDVT QFSYVDSEGSPVGVVQLTFLRLLSVSAHQDVSYPCSGAARDGPLRLRGANEDELSPETSP YVKEFRDGCQTQQGRTVLEVRTPVLEQLPVLDASFSDLGAPPRRGGVLLGPVCFMG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
