Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840329
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6603nts
Open reading frame: 1 to 6603
ATGGGGGCCATGACTCAGCTGTTGGCGGGTGTCTTTCTTGCTTTCCTTGCCCTCGCTACC
GAAGGTGGGGTGCTCAAGAAAGTCATCCGGCACAAGCGACAGAGTGGGGTGAACGCCACC
CTGCCAGAAGAGAACCAGCCAGTCGTTTTTAACCACGTTTACAACATCAAGCTGCCAGTG
GGATCCCAGTGTTCGGTGGATCTGGAGTCAGCCAGTGGGGAGAAAGACCTGGCACCGCCT
TCAGAGCCCAGCGAGAGCTTTCAGGAGCACACAGTAGATGGGGAAAACCAGATTGTCTTC
ACACATCGGATCAACATCCCCCGCCGGGCCTGTGGCTGTGCCGCAGCCCCTGATGTTAAG
GAGCTACTTAGCAGACTTGAGGAGCTGGAGAACCTGGTGTCTTCCCTGAGGGAGCAATGT
ACTGCAGGAGCAGGCTGCTGTCTCCAGCCTGCCACAGGCCGCTTGGACACCAGGCCCTTC
TGTAGCGGTCGGGGCAACTTCAGCACTGAAGGATGTGGCTGTGTCTGCGAACCCGGCTGG
AAAGGCCCCAACTGCTCTGAGCCCGAATGTCCAGGCAACTGTCACCTTCGAGGCCGGTGC
ATTGATGGGCAGTGCATCTGTGACGACGGCTTCACCGGCGAGGACTGCAGCCAGCTGGCC
TGCCCCAGCGACTGCAATGACCAGGGCAAGTGCGTAAATGGAGTCTGCATCTGCTTCGAA
GGCTACGCCGGGGCTGACTGCAGTCGTGAAATCTGCCCAGTGCCCTGCAGTGAGGAGCAC
GGCACATGTGTAGATGGCTTGTGTGTGTGCCACGATGGGTTTGCAGGCGATGACTGCAAC
AAGCCTCTGTGTCTCAACAATTGCTATAATCGTGGACGATGCGTGGAGAATGAGTGCGTG
TGTGATGAGGGTTTCACGGGCGAAGACTGCAGTGAGCTCATCTGCCCCAATGACTGCTTC
GACCGGGGACGCTGCATCAATGGCACCTGTTACTGCGAAGAAGGCTTCACAGGTGAAGAC
TGCGGGAAACCCACTTGCCCACATGCCTGCCACACCCAGGGCCGGTGTGAGGAGGGGCAG
TGTGTCTGTGATGAGGGCTTCGCCGGTGTGGACTGCTCAGAGAAGAGGTGTCCTGCTGAC
TGTCACAATCGTGGCCGCTGTGTAGACGGGCGGTGTGAGTGTGATGATGGTTTCACTGGA
GCTGACTGTGGCGAGCTCAAGTGTCCCAATGGCTGCAGTGGTCATGGACGCTGTGTAAAT
GGGCAGTGCGTGTGTGATGAGGGCTATACTGGGGAGGACTGCAGCCAGCTACGGTGCCCC
AATGACTGTCACAGCCGGGGCCGCTGTGTCGAAGGCAAATGTGTATGTGAGCAAGGCTTC
AAGGGCTATGACTGCTCCGACATGAGCTGCCCTAATGACTGTCACCAGCACGGCCGGTGT
GTTAATGGCATGTGTGTTTGTGATGACGGCTACACAGGGGAAGACTGCCGGGATCGCCAA
TGCCCCAGGGACTGCAGCAACCGCGGCCTCTGTGTGGACGGACAGTGCGTCTGTGAGGAC
GGCTTCACCGGCCCTGACTGTGCAGAACTCTCCTGTCCAAATGACTGCCATGGCCAGGGT
CGCTGTGTGAATGGGCAGTGCGTGTGCCATGAAGGATTTATGGGCAAAGACTGCAAGGAG
CAAAGATGTCCCAGTGACTGTCATGGCCAGGGCCGCTGCGTGGACGGCCAGTGCATCTGC
CACGAGGGCTTCACAGGCCTGGACTGTGGCCAGCACTCCTGCCCCAGTGACTGCAACAAC
TTAGGACAATGCGTCTCCGGCCGCTGCATCTGCAACGAGGGCTACAGCGGAGAAGACTGC
TCAGAGGTGTCTCCTCCCAAAGACCTCGTTGTGACTGAAGTGACCGAAGAGACGGTCAAC
CTGGCCTGGGACAATGAGATGCGGGTCACAGAGTACCTTGTCGTGTACACGCCCACACAC
GAGGGTGGTCTGGAAATGCAATTCCGTGTGCCTGGGGATCAGACGTCCACCATCATCCAG
GAGCTGGAGCCTGGTGTGGAGTACTTTATCCGTGTATTTGCCATCCTGGAGAACAAGAAG
AGCATTCCTGTCAGCGCCAGGGTAGCCACGTACTTACCTGCACCTGAAGGCCTGAAATTC
AAGTCCATCAAGGAGACATCTGTAGAAGTGGAGTGGGATCCTCTAGACATTGCTTTTGAA
ACCTGGGAGATCATCTTCCGGAATATGAATAAAGAAGATGAGGGAGAGATCACCAAAAGC
CTGCGGAGGCCAGAGACTTCTTACCGGCAAACTGGTCTAGCGCCCGGGCAAGAGTATGAG
ATATCTCTGCACATAGTGAAAAACAATACCCGGGGCCCTGGCCTGAAGAGGGTGACCACC
ACACGCTTGGATGCCCCCAGCCAGATCGAGGTGAAAGATGTCACAGACACCACTGCCTTG
ATTACCTGGTTCAAGCCGCTGGCTGAGATCGATGGCATTGAGCTGACATACGGCATCAAA
GACGTGCCAGGCGACCGGACCACCATCGATCTCACAGAGGACGAGAACCAGTACTCCATC
GGGAACCTGAAGCCTGACACTGAGTACGAGGTGTCCCTCATCTCCCGCAGAGGTGACATG
TCAAGCAACCCAGCCAAAGAGACATTCACAACAGGCCTGGATGCTCCCAGGAATCTTCGA
CGTGTTTCCCAGACTGATAACAGCATCACCCTGGAATGGAGGAATGGCAAGGCAGCTATT
GACAGTTACAGAATTAAGTATGCCCCCATCTCTGGAGGGGACCACGCTGAGGTTGATGTT
CCAAAGAGCCAACAAGCCACAACCAAAACCACACTCACAGGTCTGAGGCCGGGAACTGAA
TATGGGATTGGAGTTTCTGCAGTTAAGGAAGACAAGGAGAGCAATCCAGCGACCATCAAC
GCAGCCACAGAGTTGGACACGCCCAAGGACCTTCAGGTTTCTGAGACTGCAGAGACTAGC
CTGACCCTGCTCTGGAAAACACCGTTAGCCAAATTTGACCGCTACCGCCTCAATTATAGT
CTCCCCACAGGCCAGTGGGTCGGAGTGCAACTTCCAAGAAACACCACTTCCTATGTCCTG
AGGGGCCTGGAACCAGGACAGGAGTACAATGTCCTTCTGACAGCCGAGAAAGGCAGACAC
AAGAGCAAGCCCGCACGGGTGAAGGCATCCACTGAACAAGCCCCTGAGCTGGAAAACCTC
ACCGTGACTGAGGTTGGGTGGGATGGCCTCAGACTAAACTGGACCGCAGCTGATCAGGCC
TATGAGCACTTTATCATTCAGGTTCAGGAGGCCAACAAGGTGGAGGCAGCTCGGAACCTC
ACCGTGCCTGGCAGCCTTCGGGCAGTGGACATACCGGGCCTGAAGGCTGCTACGCCTTAT
ACAGTCTCCATCTATGGGGTGATCCAGGGCTATAGAACACCAGTGCTCTCTGCTGAGGCC
TCCACTGGGGAAACTCCCAATCTGGGAGAGGTCGTGGTGGCCGAGGTTGGCTGGGATGCC
CTCAAACTCAACTGGACTGCTCCAGAAGGGGCCTATGAGTACTTTTTCATTCAGGTGCAG
GAGGCTGACACAGTCGAGGCAGCCCAGAATCTCACCGTCCCTGGAGGACTGAGGTCCACA
GACCTGCCTGGGCTCAAAGCAGCCACTCATTATACCATCACCATCCGCGGGGTCACTCAG
GACTTCAGCACAACCCCTCTCTCTGTTGAAGTCTTGACAGAGGAGGTGCCAGATATGGGA
AACCTCACAGTGACCGAGGTTAGCTGGGATGCGCTCAGGCTGAACTGGACCACGCCAGAT
GGAACCTATGACCAGTTTACTATTCAGGTCCAGGAGGCTGACCAGGTGGAAGAAGCTCAC
AATCTCACGGTTCCTGGCAGCCTGCGTTCCATGGAAATCCCAGGCCTTAGGGCTGGCACT
CCTTACACAGTCACCCTGCATGGCGAGGTCAGGGGCCACAGCACTCGACCCCTTGCCGTA
GAGGTCGTCACAGAGGATCTCCCACAGCTGGGAGATTTAGCCGTGTCTGAGGTTGGCTGG
GATGGCCTCAGACTTAACTGGACCGCAGCTGACAATGCCTATGAGCACTTTGTCATTCAA
GTGCAGGAGGTCAACAAAGTGGAGGCAGCACAGAACCTCACGTTGCCTGGCAGCCTCAGG
GCTGTGGACATCCCGGGCCTCGAGGCTGCCACGCCTTATAGAGTCTCCATCTATGGGGTG
ATCCGGGGCTATAGAACACCAGTACTCTCTGCTGAGGCCTCAACAGCCAAAGAACCTGAA
ATTGGAAACTTAAATGTTTCAGACATTACTCCCGAGAGCTTCAATCTCTCCTGGATGGCG
ACCGATGGGATCTTCGAGACTTTTACCATTGAAATTATTGATTCCAATAGGTTGCTGGAG
ACTGTTGAATATAATATCTCTGGTGCCGAACGAACTGCCCATATCTCGGGGCTACCCCCC
AGTACTGACTTTATTGTCTACCTCTCTGGACTTGCTCCCAGCATCCGGACCAAAACCATT
AGTGCCACAGCCACGACAGAGGCCCTGCCCCTTCTGGAAAACCTAACCATTTCCGACATT
AATCCTTACGGATTCACAGTTTCCTGGATGGCATCGGAGAATGCCTTTGACAGCTTTCTA
GTAACGGTGGTGGATTCTGGGAAGCTACTGGACCCCCAGGAATTCACACTTTCAGGAACC
CAGAGGAAGCTAGAGCTGAGAGGCCTCATCACTGGCATTGGCTATGAGGTTATGGTGTCT
GGCTTCACCCAAGGGCATCAAACCAAGCCCTTGAGGGCTGAGATTGTTACAGAAGCCGAA
CCGGAAGTTGACAACCTCCTGGTTTCAGATGCCACCCCAGACGGTTTCCGTCTGTCCTGG
ACAGCTGATGAAGGCGTGTTCGACAATTTTGTTCTCAAAATCAGAGATACCAAAAAGCAG
TCTGAGCCACTGGAAATAACCCTACTTGCCCCCGAACGTACTAGAGACATAACAGGACTC
AGAGAGGCTACTGAATACGAAATTGAACTCTATGGCATAAGCAAGGGAAGGCGATCCCAG
ACAGTCAGTGCTATAGCAACCACAGCAATGGGCTCCCCAAAGGAAGTCATTTTCTCAGAC
ATCACTGAAAATTCGGCTACTGTCAGCTGGAGAGCACCCACAGCACAAGTGGAGAGCTTC
CGGATTACCTATGTGCCTATCACAGGCGGTACACCTTCCATGGTAACTGTGGACGGAACC
AAGACTCAGACCAGGCTGGTGAAACTCATACCTGGCGTGGAGTATCTTGTTAGCATCATT
GCCATGAAGGGCTTTGAGGAAAGTGAACCTGTCTCAGGGTCATTCACCACAGCTCTGGAT
GGCCCATCTGGCCTGGTTACAGCCAACATTACTGACTCAGAAGCCTTGGCCAGGTGGCAG
CCAGCCATTGCCACTGTGGACAGTTATGTCATCTCTTACACAGGCGAGAAAGTGCCAGAA
ATTACACGGACGGTGTCCGGGAACACAGTGGAGTATGCTCTGACCGATCTCGAGCCTGCC
ACGGAATACACACTGAGAATCTTTGCAGAGAAAGGGCCCCAGAAGAGCTCAACCATCACT
GCCAAGTTCACAACAGACCTCGATTCTCCAAGAGACTTGACTGCTACTGAGGTTCAGTCG
GAAACTGCCCTCCTTACCTGGCGACCCCCCCGGGCATCAGTCACAGGTTACCTGCTGGTC
TATGAATCAGTGGATGGCACAGTCAAGGAAGTCATTGTGGGTCCAGATACCACCTCCTAC
AGCCTGGCGGACCTGAGCCCATCCACCCACTACACAGCCAAGATCCAGGCGCTCAATGGG
CCCCTGAGGAGCAATATGATCCAGACCATCTTCACCACAATTGGACTCCTGTACCCCTTC
CCCAAGGACTGCTCCCAAGCAATGCTGAATGGTGACACGACCTCCGGCCTGTACACCATT
TATCTGAATGGCGATAAGGCTGAGGCGCTGGAAGTCTTCTGTGACATGACCTCTGATGGG
GGTGGATGGATTGTGTTCCTGAGACGCAAGAACGGACGCGAGAACTTCTACCAAAACTGG
AAGGCATATGCTGCTGGATTTGGGGACCGCAGAGAAGAATTCTGGCTTGGGCTGGACAAC
CTGAACAAAATCACAGCCCAGGGGCAGTACGAGCTGCGGGTGGACCTGCGGGACCATGGG
GAGACAGCATTTGCTGTCTATGACAAGTTCAGCGTGGGAGATGCCAAGACTCGCTACAAG
CTGAAGGTGGAGGGATACAGTGGGACAGCCGGTGACTCCATGGCCTACCACAATGGCAGA
TCCTTCTCCACCTTTGACAAGGACACAGATTCAGCCATCACCAACTGTGCTCTGTCCTAC
AAAGGGGCTTTCTGGTATAGGAACTGTCACCGAGTCAACCTGATGGGGAGATATGGGGAC
AATAACCACAGTCAGGGCGTCAATTGGTTCCACTGGAAGGGCCATGAACACTCTATCCAG
TTTGCTGAGATGAAGCTGCGGCCAAGTAACTTCAGAAATCTTGAAGGCAGGCGCAAAAGG
GCA
Protein sequence predicted based on nucleotide sequence
MGAMTQLLAGVFLAFLALATEGGVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIKLPV GSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVK ELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVCEPGW KGPNCSEPECPGNCHLRGRCIDGQCICDDGFTGEDCSQLACPSDCNDQGKCVNGVCICFE GYAGADCSREICPVPCSEEHGTCVDGLCVCHDGFAGDDCNKPLCLNNCYNRGRCVENECV CDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGKPTCPHACHTQGRCEEGQ CVCDEGFAGVDCSEKRCPADCHNRGRCVDGRCECDDGFTGADCGELKCPNGCSGHGRCVN GQCVCDEGYTGEDCSQLRCPNDCHSRGRCVEGKCVCEQGFKGYDCSDMSCPNDCHQHGRC VNGMCVCDDGYTGEDCRDRQCPRDCSNRGLCVDGQCVCEDGFTGPDCAELSCPNDCHGQG RCVNGQCVCHEGFMGKDCKEQRCPSDCHGQGRCVDGQCICHEGFTGLDCGQHSCPSDCNN LGQCVSGRCICNEGYSGEDCSEVSPPKDLVVTEVTEETVNLAWDNEMRVTEYLVVYTPTH EGGLEMQFRVPGDQTSTIIQELEPGVEYFIRVFAILENKKSIPVSARVATYLPAPEGLKF KSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYE ISLHIVKNNTRGPGLKRVTTTRLDAPSQIEVKDVTDTTALITWFKPLAEIDGIELTYGIK DVPGDRTTIDLTEDENQYSIGNLKPDTEYEVSLISRRGDMSSNPAKETFTTGLDAPRNLR RVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHAEVDVPKSQQATTKTTLTGLRPGTE YGIGVSAVKEDKESNPATINAATELDTPKDLQVSETAETSLTLLWKTPLAKFDRYRLNYS LPTGQWVGVQLPRNTTSYVLRGLEPGQEYNVLLTAEKGRHKSKPARVKASTEQAPELENL TVTEVGWDGLRLNWTAADQAYEHFIIQVQEANKVEAARNLTVPGSLRAVDIPGLKAATPY TVSIYGVIQGYRTPVLSAEASTGETPNLGEVVVAEVGWDALKLNWTAPEGAYEYFFIQVQ EADTVEAAQNLTVPGGLRSTDLPGLKAATHYTITIRGVTQDFSTTPLSVEVLTEEVPDMG NLTVTEVSWDALRLNWTTPDGTYDQFTIQVQEADQVEEAHNLTVPGSLRSMEIPGLRAGT PYTVTLHGEVRGHSTRPLAVEVVTEDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQ VQEVNKVEAAQNLTLPGSLRAVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEASTAKEPE IGNLNVSDITPESFNLSWMATDGIFETFTIEIIDSNRLLETVEYNISGAERTAHISGLPP STDFIVYLSGLAPSIRTKTISATATTEALPLLENLTISDINPYGFTVSWMASENAFDSFL VTVVDSGKLLDPQEFTLSGTQRKLELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVTEAE PEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPERTRDITGL REATEYEIELYGISKGRRSQTVSAIATTAMGSPKEVIFSDITENSATVSWRAPTAQVESF RITYVPITGGTPSMVTVDGTKTQTRLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTTALD GPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVEYALTDLEPA TEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYLLV YESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPF PKDCSQAMLNGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNW KAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYK LKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGD NNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
