Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840359
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4617nts
Open reading frame: 1 to 4617
ATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAACTCCTTGATGATTTTGAA
CAGAACCCCGATGAACAAGATTATCTCCAAGATGTGCAAAATGCATATGATTCTAACCAC
TGCTCAGTTTCTTCAGAGTTGGCTTCCTCACAGCGAACTTCATTGCTCCCAAAAGACCAG
GAGTGCGTTAATAGTTGTGCCTCATCAGAAACTAGCTACGGAACAAATGAGAGTTCCCTG
AATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATGAAAAAAATGTAACAGGACTT
GATCTTCTGTCTTCTGTGGATGGTGGTACTTCCGATGAAATCCAGCCGTTGTACATGGGA
CGATGTTCTAAACCTATTTGTGATCTGATAAGTGACATGGGTAACTTAGTTCATGCAACC
AATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTTAAGTCTAATGCCGATTCC
TTGATTGGATTGGATTTATCTTCAGTGTCAGATACTCCCTGTGTTTCTTCAACAGACCAT
GATAGCGATACTGTCAGAGAACAACAGAATGATATCAGTTCTGAATTACAGAATAGAGAA
ATCGGAGGAATCAAAGAATTGGGTATAAAAGTAGATACAACACTTTCAGATTCCTACAAT
TACAGTGGCACAGAAAATCTAAAAGATAAGAAGATCTTCAATCAGTTGGAATCAATTGTT
GATTTTAACATGTCATCCGCTTTGACTCGACAAAGTTCCAAAATGTTTCATGCCAAAGAC
AAGCTGCAACACAAGAGCCAGCCATGTGGACTACTCAAAGATGTTGGCTTAGTAAAAGAG
GAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAAGAAGAGGGCAAGACAAGT
GCATTGACCTGCAGCCTTCCGAAAAACGAAGATTTGTGCTTAAATGATTCAAATTCAAGA
GATGAAAATTTCAAGTTACCTGACTTCTCCTTTCAGGAAGATAAGACTGTTATAAAGCAA
TCTGCACAAGAAGACTCAAAAAGTTTGGACCTTAAGGATAATGATGTAATCCAAGATTCC
TCTTCAGCTCTGCATGTTTCCAGTAAAGATGTGCCGTCTTCACTGTCCTGTCTTCCTGCG
TCTGGGTCTATGTGTGGCTCATTAATTGAAAGCAAAGCACGGGGTGATTTTTTACCTCAG
CATGAACATAAAGATAATATACAAGATGCAGTGACTATACATGAAGAAATACAGAACAGT
GTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTGTTGAAACAGGAAAAATGTAAA
AGCATACTGCTTCAGTCATTAATTGAAGGGATGGAAGACAGAAAGATAGATCCCGACCAG
ACAGTAATCAGAGCCGAGTCTTTGGATGGCGGTGACACCAGTTCTACAGTTGTAGAATCT
CAAGAGGGGCTTTCTGGCACTCATGTCCCAGAGTCTTCTGATTGTTGTGAAGGTTTTATT
AATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAGATTACTTTAATATTGATGAA
GGCGCAAAAAGTGGCCCACTAATTAGCGATGCTGAACTTGATGCCTTTCTGACAGAACAG
TATCTTCAGACCACTAACATAAAGTCCTTTGAAGAGAATGTAAATGACTCGAAATCGCAA
ATGAATCAGATAGATATGAAGGGCTTAGATGATGGAAACATCAACAATATATATTTCAAT
GCAGAAGCAGGAGCTATTGGGGAAAGTCATGGTATTAATATAATTTGCGAAACTGTTGAT
AAACAAAATACCATAGAAAATGGCCTTTCTTTAGGAGAAAAAAGTACTATTCCCGTTCAA
CAAGGGTTACCTACCAGTAAGTCTGAGATTACAAATCAATTGTCAGTCTCTGATATTAAC
AGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTAGCCTTCCATCACGAACAAGG
AGTTCAAAGGACCTGAATAAGCCAGATGTGCCAGATACAATAGAAAGTGAGCCCAGCACA
GCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACAGCTGATCCCCAGGTTAGC
TTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGTGGATCTAGTTTCGTAACT
GCAAATGAAGATTCTGTACCTGAAAACACTTGCAAAGAAGGATTGGTTTTGGGCCAGAAA
CAGCCTACTTGGGTTCCTGATTCAGAAGCACCAAACTGTATGAACTGCCAAGTGAAATTT
ACTTTTACCAAACGGCGACACCATTGCCGAGCATGTGGGAAAGTATTTTGTGGTGTTTGT
TGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAGCAAGAGTATGTGTGGTCTGC
TATGAAACTATTAGTAAAGCTCAGGCATTTGAAAGGATGATGAGCCCAACTGGTTCTAAT
CTGAAGTCTAATCATTCTGATGAATGTACTACTGTCCAGCCACCTCAGGAGAACCAAACA
TCCAGTATACCTTCACCAGCAACCTTGCCAGTGTCCGCACTTAAACAACCAGGCGTTGAA
GGACTATGTTCCAAAGAACAGAAGAGAGTGTGGTTTGCAGATGGTATATTGCCCAATGGC
GAAGTGGCAGATACAACAAAATTATCATCCGGAAGTAAAAGATGTTCCGAAGACTTTAGT
CCTCTTTCACCTGATGTGCCTATGACAGTGAACACAGTGGATCATTCCCATTCTACTACA
GTGGAAAAGCCAAATAATGAGACTGGAGATATTACAAGAAATGAGATAATTCAGAGTCCT
ATTTCACAGGTTCCATCAGTGGAAAAATTGTCTATGAACACAGGGAATGAGGGGTTACCT
ACTTCTGGTTCATTTACACTAGATGATGATGTTTTTGCAGAAACTGAAGAACCATCTAGT
CCTACTGGTGTCTTAGTTAACAGCAATTTACCTATCGCTAGTATTTCAGATTATAGGTTA
CTGTGTGATATTAACAAGTATGTCTGCAATAAGATTTCCCTTCTACCTAATGATGAGGAC
AGTTTGCCCCCCCTCCTGGTTGCATCTGGAGAAAAGGGATCAGTGCCTGTAGTAGAAGAA
CATCCATCTCATGAGCAGATTATTTTGCTTCTTGAAGGTGAAGGCTTTCATCCTGTTACA
TTTGTCCTCAATGCTAATCTACTCGTGAATGTGAAATTCATATTTTATTCTTCAGACAAG
TATTGGTACTTTTCAACCAATGGATTGCATGGATTGGGACAGGCAGAAATTATTATTCTA
TTGTTATGTTTGCCAAATGAGGATACTATTCCTAAGGACATCTTCAGACTATTTATCACC
ATCTATAAGGATGCTCTAAAAGGAAAATACATAGAAAACTTGGACAATATTACCTTTACT
GAGAGTTTTCTCAGTAGCAAGGATCACGGCGGCTTCCTGTTTATTACACCTACTTTTCAG
AAACTTGATGATCTCTCATTACCAAGCAATCCTTTTCTTTGTGGAATTCTTATCCAGAAG
CTTGAGATCCCCTGGGCAAAGGTTTTTCCTATGCGTTTAATGTTGAGATTGGGTGCAGAA
TATAAAGCATATCCTGCTCCTCTAACAAGTATCAGAGGCCGCAAACCTCTCTTTGGAGAA
ATAGGACACACTATTATGAACTTACTTGTGGACCTTCGAAATTACCAGTATACCTTGCAT
AATATAGATCAACTGTTGATTCATATGGAAATGGGAAAAAGCTGCATAAAAATACCACGG
AAAAAGTACAGTGATGTAATGAAAGTACTAAATTCTTCCAATGAGCATGTCATTAGTATT
GGCGCAAGTTTCAGTACCGAAGCAGATTCTCATCTGGTCTGTATACAGAATGATGGAATT
TATGAAACACAGGCCAATAGTGCAACTGGCCATCCTAGAAAAGTGACAGGTGCAAGTTTT
GTGGTGTTCAATGGAGCTCTAAAAACATCCTCAGGATTTCTTGCTAAGTCCAGCATAGTT
GAAGATGGCTTAATGGTGCAAATAACTCCAGAGACAATGAATGGCTTACGGCTAGCTTTA
CGAGAACAGAAAGACTTTAAAATTACATGTGGGAAAGTTGATGCAGTAGACCTGAGAGAA
TACGTGGATATCTGCTGGGTAGATGCTGAAGAAAAAGGAAACAAAGGAGTTATCAGTTCT
GTTGATGGAATATCATTACAAGGATTTCCAAGTGAAAAAATCAAACTTGAAGCAGATTTT
GAAACCGATGAGAAGATTGTAAAATGTACCGAGGTCTTCTACTTTCTCAAGGACCAGGAT
TTATCTATTTTATCAACTTCTTATCAGTTTGCAAAAGAAATAGCCATGGCCTGTAGTGCT
GCGCTGTGCCCTCACCTGAAAACTCTAAAAAGTAATGGGATGAATAAAATTGGACTTCGA
GTCTCTATTGATACTGATATGGTTGAATTTCAGGCAGGATCTGAAGGCCAGCTGCTGCCT
CAGCATTATCTAAATGATCTTGATAGTGCTCTGATACCTGTGATCCATGGTGGGACCTCC
AACTCTAGCTTGCCATTAGAAATAGAATTAGTGTTTTTCATTATAGAACATCTGTTT
Protein sequence predicted based on nucleotide sequence
MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQ ECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMG RCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDH DSDTVREQQNDISSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIV DFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTS ALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLDLKDNDVIQDS SSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNS VVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVES QEGLSGTHVPESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQ YLQTTNIKSFEENVNDSKSQMNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVD KQNTIENGLSLGEKSTIPVQQGLPTSKSEITNQLSVSDINSQSVGGARPKQLFSLPSRTR SSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVSFNSNYIDIESNSEGGSSFVT ANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCRACGKVFCGVC CNRKCKLQYLEKEARVCVVCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQT SSIPSPATLPVSALKQPGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFS PLSPDVPMTVNTVDHSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLP TSGSFTLDDDVFAETEEPSSPTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDED SLPPLLVASGEKGSVPVVEEHPSHEQIILLLEGEGFHPVTFVLNANLLVNVKFIFYSSDK YWYFSTNGLHGLGQAEIIILLLCLPNEDTIPKDIFRLFITIYKDALKGKYIENLDNITFT ESFLSSKDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFPMRLMLRLGAE YKAYPAPLTSIRGRKPLFGEIGHTIMNLLVDLRNYQYTLHNIDQLLIHMEMGKSCIKIPR KKYSDVMKVLNSSNEHVISIGASFSTEADSHLVCIQNDGIYETQANSATGHPRKVTGASF VVFNGALKTSSGFLAKSSIVEDGLMVQITPETMNGLRLALREQKDFKITCGKVDAVDLRE YVDICWVDAEEKGNKGVISSVDGISLQGFPSEKIKLEADFETDEKIVKCTEVFYFLKDQD LSILSTSYQFAKEIAMACSAALCPHLKTLKSNGMNKIGLRVSIDTDMVEFQAGSEGQLLP QHYLNDLDSALIPVIHGGTSNSSLPLEIELVFFIIEHLF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
