Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840360
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4632nts
Open reading frame: 1 to 4632
ATGGAGGCTGCAACAACACTTCACCCAGGACCTCGCCCTGCTCTTCCCCTTGGTGGCCCT
GGCCCTTTGGGAGAATTCCTGCCTCCACCCGAGTGTCCGGTTTTCGAACCCAGCTGGGAA
GAGTTTGCGGACCCCTTCGCCTTCATCCACAAGATCAGGCCCATAGCCGAGCAGACTGGC
ATCTGTAAGGTGCGGCCGCCGCCGGATTGGCAGCCACCATTTGCATGTGATGTTGATAAA
CTGCATTTTACGCCACGGATCCAGAGACTGAATGAATTAGAGGCCCAAACTCGTGTAAAA
TTGAATTTCCTGGACCAGATTGCAAAGTACTGGGAGCTACAGGGAAGTACTCTCAAAATT
CCACATGTCGAGAGGAAGATCCTGGACTTATTTCAGCTTAATAAGTTAGTTGCAGAAGAA
GGTGGATTTGCCGTGGTTTGCAAGGATAGAAAATGGACCAAAATTGCTACCAAGATGGGG
TTTGCTCCTGGCAAAGCAGTGGGCTCACATATCAGAGGGCATTATGAACGAATTCTCAAC
CCCTACAACTTATTCCTGTCTGGCGACAGCCTAAGGTGTTTGCAGAAGCCAAACCTGACC
ACAGACACTAAGGACAAGGAGTACAAACCCCATGATATTCCCCAGAGGCAGTCAGTGCAG
CCTTCGGAAACGTGCCCCCCCGCCCGACGAGCAAAACGCATGAGAGCAGAGGCCATGAAT
ATTAAAATAGAACCCGAGGAGACAACGGAAGCCAGAACTCATAATCTGAGACGTAGAATG
GGTTGTCCAACTCCTAAATGTGAGAACGAGAAAGAAATGAAGAGTAGCATCAAGCAAGAA
CCTATTGAGAGGAAAGATTATATTGTAGAAAACGAGAAGGAAAAGCCCAAGAGTCGATCT
AAAAAAGCCACAAATGCTGTGGACCTGTATGTCTGTCTGTTATGTGGGAGTGGCAATGAT
GAAGACCGGCTACTGCTGTGTGATGGCTGTGATGACAGTTACCATACCTTTTGCTTGATC
CCACCTCTACATGATGTTCCCAAGGGCGACTGGAGGTGTCCTAAGTGTTTGGCTCAGGAA
TGTAGTAAGCCACAAGAAGCATTTGGCTTTGAACAAGCAGCTAGGGACTATACCCTCCGG
ACTTTTGGGGAAATGGCAGATGCGTTCAAATCTGATTACTTCAACATGCCAGTCCATATG
GTGCCCACAGAGCTTGTGGAGAAAGAATTTTGGAGACTGGTATCCACTATTGAGGAGGAT
GTTACAGTCGAATATGGCGCTGACATTGCCTCAAAGGAATTTGGCAGTGGATTTCCTGTC
CGAGATGGGAAAATCAAACTCTCACCTGAGGAAGAGGAGTATCTGGATAGTGGCTGGAAT
TTGAACAACATGCCAGTGATGGAGCAGTCTGTCCTTGCACATATTACTGCTGATATATGT
GGCATGAAACTTCCTTGGTTGTATGTGGGAATGTGCTTTTCTTCATTCTGTTGGCACATT
GAAGACCACTGGAGCTATTCAATTAACTACTTGCACTGGGGTGAGCCAAAGACCTGGTAT
GGAGTGCCAGGGTATGCTGCTGAGCAGCTGGAAAATGTAATGAAGAAACTAGCTCCAGAA
CTCTTTGTGTCACAGCCGGATCTCCTCCATCAGCTTGTGACCATCATGAACCCCAATACC
CTGATGACTCATGAAGTGCCTGTTTACCGAACTAATCAGTGTGCTGGGGAGTTTGTGATT
ACCTTCCCAAGAGCCTACCACAGTGGTTTTAACCAGGGTTTTAATTTTGCTGAGGCTGTT
AACTTCTGCACTGTTGATTGGCTGCCATTAGGCCGACAGTGTGTGGAGCATTATCGCTTG
CTTCATCGATATTGTGTGTTTTCCCACGATGAGATGATCTGCAAGATGGCCTCCAAGGCT
GATGTATTAGATGTTGTAGTGGCTTCTACTGTTCAGAAAGACATGGCCATTATGATTGAG
GATGAGAAAGCTTTAAGAGAGACTGTCCGTAAATTGGGAGTGATTGATTCGGAAAGAATG
GATTTTGAGCTATTGCCAGATGATGAACGGCAGTGTGTAAAATGCAAAACTACATGCTTC
ATGTCTGCCATCTCCTGTTCTTGTAAACCTGGCCTTCTGGTTTGCCTGCATCATGTAAAG
GAATTGTGTTCCTGTCCTCCTTACAAATATAAATTGCGGTATAGGTACACGCTGGATGAT
CTCTACCCTATGATGAATGCATTGAAGCTTAGAGCAGAATCTTACAACGAATGGGCCCTG
AATGTGAATGAAGCTTTGGAGGCAAAGATCAACAAGAAGAAAAGCCTTGTCAGCTTCAAG
GCTTTAATTGAAGAATCTGAAATGAAGAAATTCCCAGACAATGATCTTTTGCGACACCTT
CGCCTAGTCACACAGGATGCAGAGAAGTGTGCCTCTGTTGCGCAGCAGCTGCTTAATGGC
AAACGCCAAACTAGATATCGATCTGGCGGAGGGAAATCCCAAAATCAGTTGACAGTGAAT
GAGCTCCGGCAGTTTGTAACACAGCTGTATGCTCTGCCATGTGTCCTCAGTCAGACACCC
TTACTGAAGGATCTCTTGAATCGTGTAGAAGATTTTCAACAGCATAGCCAGAAACTACTC
TCCGAGGAAACGCCTAGTGCTGCGGAGCTGCAGGACTTGCTGGATGTCAGCTTTGAATTT
GATGTGGAACTTCCACAGCTTGCTGAGATGCGTATCCGTTTGGAACAAGCCCGTTGGCTT
GAAGAGGTGCAGCAAGCTTGCCTAGACCCCAGCTCCCTCACTCTCGATGATATGCGACGT
CTCATAGACCTAGGGGTAGGCCTGGCCCCCTACTCAGCAGTGGAGAAAGCTATGGCTCGG
CTGCAGGAACTGCTGACAGTGTCAGAGCACTGGGACGACAAAGCCAAGAGTCTCCTCAAG
GCCAGGCCACGGCATTCATTGAATAGCCTGGCTACGGCAGTAAAGGAAATCGAAGAGATC
CCTGCATATCTGCCCAATGGTGCGGCTCTGAAAGACTCAGTGCAGAGAGCCAGAGACTGG
CTTCAGGATGTAGAGGGCCTGCAGGCTGGAGGCCGGGTGCCAGTGTTAGACACCCTCATA
GAACTTGTTACACGGGGCCGATCGATCCCTGTACATCTGAATTCTTTGCCAAGGCTCGAA
ACCCTAGTAGCCGAGGTTCAGGCTTGGAAAGAATGTGCTGTTAATACATTCTTGACCGAG
AATTCTCCATATTCTCTCTTAGAGGTGCTGTGTCCCCGATGTGATATTGGCCTTTTGGGA
TTGAAAAGGAAGCAGAGAAAGTTAAAGGAGCCACTCCCAAATGGAAAGAAAAAAAGCACC
AAATTGGAGAGTCTGAGCGACCTCGAGAGAGCTTTGACTGAAAGCAAGGAGACTGCCTCA
GCTATGGCAACTCTTGGGGAAGCCCGCCTGAGGGAAATGGAAGCCTTGCAGTCCCTCAGA
CTCGCCAATGAAGGGAAATTGCTGTCGCCTCTTCAAGATGTGGATATAAAAATCTGCCTA
TGTCAGAAGGCCCCAGCTGCCCCTATGATTCAATGTGAACTCTGCAGGGATGCTTTCCAC
ACCAGTTGTGTGGCGGTACCCAGTATTTCACAGGGACTGCGAATCTGGCTTTGTCCCCAT
TGTCGGAGGTCAGAGAAACCTCCATTAGAGAAAATTCTCCCCCTGCTCGCCTCCCTCCAG
CGTATCCGAGTGCGCCTCCCTGAGGGAGATGCACTTCGATATATGATCGAAAGAACCGTG
AACTGGCAGCACAGGGCCCAGCAACTGTTGTCGTCAGGGAATCTGAAATTTGTCCAAGAT
CGAGTGGGATCAGGACTGTTATATAGCAGATGGCAAGCCTCAGCAGGACAGGTGTCAGAC
ACAAACAAGGTATCTCAACCTCCTGGCACAACATCTTTCTCTTTACCTGATGACTGGGAC
AACAGAACCTCATATCTGCACTCCCCCTTCTCCACTGGACGAAGTTGTATCCCCCTCCAT
GGTGTTAGTCCCGAAGTGAATGAACTGTTGATGGAAGCCCAGCTGCTCCAGGTATCCCTT
CCTGAAATTCAGGAACTTTACCAGACTTTACTTGCAAAGCCATCTCCTGCTCAGCAGACT
GACCGAAGTTCACCAGTCAGACCCAGCAGTGAGAAGAATGACTGTTGTCGGGGGAAGCGA
GATGGAATTAACAGTCTTGAGAGAAAACTCAAGAGACGCCTTGAAAGAGAGGGCCTTTCC
AGTGAGCGGTGGGAACGAGTTAAGAAAATGCGGACTCCCAAAAAGAAGAAAATCAAACTG
AGCCACCCTAAGGACATGAACAATTTCAAGTTGGAGAGAGAGCGTAGCTATGAATTAGTT
CGTTCTGCTGAAACCCATTCCCTGCCCTCAGACACATCCTATTCCGAACAGGAAGACTCT
GAGGATGAAGACGCTATCTGCCCAGCTGTGAGCTGCCTGCAGCCAGAAGGAGATGAGGTG
GACTGGGTCCAGTGTGATGGCAGCTGCAATCAGTGGTTTCATCAGGTCTGTGTTGGTGTC
TCTCCAGAGATGGCAGAGAAAGAAGACTACATCTGTGTGCGCTGTACTGTGAAGGACGCC
CCAAGCCGAAAG
Protein sequence predicted based on nucleotide sequence
MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTG ICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKI PHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILN PYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMN IKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRS KKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQE CSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEED VTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADIC GMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPE LFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAV NFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIE DEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVK ELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFK ALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVN ELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEF DVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMAR LQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDW LQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTE NSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETAS AMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFH TSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTV NWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWD NRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQT DRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKL SHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEV DWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
