Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840395
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4158nts
Open reading frame: 1 to 4158
ATGCCTGAGGGAGCCCAAGGACTGAGCCTCTCCAAACCTAGCCCAAGCCTCGGGTGTGGC
CGAAGAGGTGAAGTCTGTGACTGTGGCACCGTGTGTGAGACTCGGACAGCTCCTGCAGCC
CCCACCATGGCCTCCCCCCGAGGTTCAGGGAGCTCCACATCGCTGAGCACAGTGGGCTCT
GAGGGGGATCCCGCCCCTGGGCCCACTCCAGCCTGCTCAGCCTCCAGGCCAGAGCCCCTT
CCAGGGCCTCCCATCCGCCTACATCTCTCTCCGGTGGGGATCCCAGGTTCAGCCAGACCT
TCAAGGCTGGAGCGTGTGGCTCGGGAGATCGTGGAGACAGAACGGGCCTATGTCAGGGAC
CTGCGCAGCATCGTGGAGGACTACCTGGGCCCTCTGCTGGACGGCGGGGTCCTGGGGCTG
AGCGTGGAGCAGGTCGGCACGCTGTTTGCCAACATTGAGGACATCTACGAGTTCAGCAGC
GAGCTCCTGGAGGACTTGGAGAACAGCAGCAGCGCCGGGGGTATTGCCGAGTGCTTCGTG
CAGAGGAGCGAGGATTTTGACATCTACACATTGTACTGCATGAACTACCCGAGCTCCCTG
GCCCTGCTTCGGGAGCTGTCGTTGTCTCCGCCAGCAGCCCTGTGGCTGCAGGAGCGCCAG
GCCCAGCTTCGCCACTCGCTGCCCCTGCAGAGCTTCCTGCTGAAACCTGTCCAGCGCATT
CTCAAGTACCATCTGCTGCTGCAGGAACTAGGGAAGCACTGGGCGGAGGGACCAGGCACT
GGGGGTCGCGAGATGGTGGAGGAAGCTATTGTGTCTATGACAGCGGTTGCTTGGTACATC
AACGACATGAAGCGCAAGCAGGAGCATGCAGCGCGCCTCCAGGAAGTGCAGCGGCGGCTG
GGTGGATGGACCGGACCAGAGCTCAGTGCTTTTGGGGAACTGGTGTTGGAGGGCGCGTTC
CGAGGAGGCGGAGGGGGCGGCCCCCGGCTACGAGGGGGTGAGAGGCTGCTCTTCCTGTTC
TCTCGGATGCTGCTGGTGGCCAAGCGCAGGGGGCTGGAGTACACCTACAAAGGCCACATC
TTCTGCTGCAACCTGAGCGTGAGCGAGAGTCCCCGGGACCCCCTAGGGTTCAAGGTGTCT
GATCTGACCATTCCCAAGCACAGACACCTCCTCCAGGCCAAGAACCAAGAAGAGAAGAGG
CTGTGGATTCACTGTCTCCAGCGCCTCTTCTTTGAGAACCACCCTGCCTCCATCCCTGCC
AAGGCAAAGCAAGTTCTCCTTGAAAACAGTCTGCATTGTGCCCCCAAAAGTAAGCCTGTC
CTAGAGCCCCTGACACCCCCACTTGGCTCTCCTCGACCTCGAGATGCTAGAAGTTTTACC
CCTGGGAGAAGGAACACCGCTCCATCTCCTGGGCCCTCTGTGATTCGCCGAGGCCGGAGG
CAGTCTGAGCCGGTGAAGGACCCTTATGTCATGTTCCCACAGAACGCTAAGCCTGGATTC
AAGCACGCTGGCAGCGAAGGGGAACTGTACCCTCCAGAATCCCAGCCACCAGTTTCAGGC
TCTGCACCCCCTGAGGACCTGGAGGATGCTGGACCCCCAACACTGGACCCCTCTGGGACC
TCAATCACTGAAGAAATCCTGGAACTGCTGAATCAGCGAGGCCTTCGAGATCCAGGGCCG
TCCACCCATGACATTCCCAAGTTCCCCGGAGACTCCCAGGTGCCTGGCGACAGCGAAACC
CTCACATTCCAAGCCCTGCCCAGCCGGGACTCTTCAGAAGAGGAGGAGGAGGAAGAGGAA
GGGCTGGAGATGGATGAACGGGGGCCTTCCCCACTCCACGTCCTGGAAGGGCTGGAAAGT
TCTATTGCAGCTGAAATGCCCAGCATTCCCTGCCTTACCAAAATTCCTGACGTGCCCAAC
CTTCCTGAAATTCCCAGTCGGTGTGAAATTCCCGAAGGTTCTCGCCTTCCTAGTCTCTCT
GACATTTCCGATGTTTTTGAGATGCCCTGCCTTCCAGCCATACCTAGTGTCCCCAACACC
CCCAGTCTGTCTAGCACTCCCACCCTCTCCTGTGACTCCTGGCTCCAAGGGCCTCTGCAG
GAACCAGCTGAGGCTCCAGCCACCAGGAGAGAATTGTTTTCTGGGAGCAATCCTGGGAAA
CTGGGAGAGCCGCCTTCAGGAGGCAAGGCAGGGCCAGAGGAGGATGAAGAAGGGGTATCA
TTCACAGACTTCCAGCCCCAGGATGTCACCCAACATCAGGGATTCCCAGATGAGCTGGCA
TTCCGCTCTTGCTCAGAAATCCGGAGCGCCTGGCAGGCATTGGAACAGGGACAGCTGGCC
CGGCCAGGCTTCCCAGAGCCACTGCTCATCCTGGAGGATTCGGATCTGGGTGGAGACAGC
GGGAGCGGGAAGGCAGGAGCCCCGAGTTCAGAAAGGACGGCGTCCAGAGTGCGAGAGCTG
GCCCGGCTTTACAGCGAGCGGATCCAGCAGATGCAGAGGGCGGAGACTCGGGCCTCAGCC
AATGCCCCGCGACGCCGGCCTCGGGTTCTTGCCCAACCCCAGCCATCCCCCTGTCTTCCC
CAGGAGCAGGCAGAGCCAGGGCTCCTGCCTGCCTTTGGACACGTGCTGGTATGTGAGCTG
GCCTTCCCACTGACATGTGCCCAGGAGTCTGTCCCCCTGGGTCCTGCTGTCTGGGTTCAA
GCTGCCATACCTTTGTCAAAGCAGGGAGGCAGCCCCGATGGCCAGGGTCTACATGTTTCC
AATTTGCCTAAGCAAGACCTTCCGGGCATCCACGTTTCAGCTGCTACCCTTTTGCCTGAG
CAAGGAGGTTCCCGGCATGTCCAGGCTCCAGCCGCCACACCTTTGCCTAAGCAAGAAGGC
CCCCTGCACCTCCAGGTGCCGGCTCTTACAACCTTCTCTGATCAAGGCCACCCAGAAATC
CAAGTTCCAGCCACCACTCCTTTGCCTGAGCATAGAAGTCACATGGTTATACCAGCTCCA
TCCACCGCCTTTTGTCCTGAGCAGGGACACTGTGCGGACATCCACGTTCCTACCACTCCA
GCTTTGCCTAAGGAGATTTGTTCTGATTTCACAGTTTCAGTCACCACCCCTGTGCCCAAG
CAAGAAGGTCACCTAGACAGCGAGAGCCCAACCAATATCCCACTGACCAAGCAAGGAGGT
TCCAGGGATGTTCAGGGCCCAGACCCTGTCTGCAGTCAACCCATCCAGCCCTTGTCCTGG
CATGGAAGCAGCCTGGATCCCCAGGGCCCAGGCGACACCCTACCACCCTTGCCATGTCAC
CTCCCAGACCTTCAGATTCCAGGTACCTCACCTTTGCCTGCACATGGAAGCCACCTCGAC
CATCGGATCCCAGCCAACGCCCCACTCTCTTTGTCACAGGAGTTGCCAGACACTCAGGTT
CCAGCTACCACACCTTTGCCCCTGCCACAAGTCCTCACAGACATCTGGGTCCAAGCCCTC
CCAACTTCACCCAAGCAGGGAAGCCTCCCAGACATCCAGGGTCCAGCGGCTGCACCTCCA
CTTCCGGAGCCAAGCCTTACAGATACACAGGTCCAAAAACTCACACCCTCGTTGGAGCAG
AAGAGCCTCATAGATGCCCATGTTCCAGCTGCCACACCTTTACCTGAGAGAGGAGGCTCT
CTAGACATTCAGGGCCTCTCACCCACCCCAGTTCAGACCACCATGGTTTTGTCCAAACCA
GGAGGCTCCTTAGCCTCTCACGTTGCCAGGTTGGAGTCTTCAGACTTGACGCCACCTCAT
AGTCCCCCACCTTCCAGCCGTCAGCTGCTGGGACCCAATGCAGCTGCCCTCTCTAGATAC
CTGGCAGCCTCATATATCAGCCAGAGTCTGGCTCGGCGGCAGGGACCTGGAGGAGGGGCC
CCCGCAGCCTCTCGGGGCTCTTGGTCTTCTGCTCCCACATCAAGGGCATCTTCGCCGCCT
CCCCAGCCCCAGCCACCACCTCCTCCAGCCAGGCGGCTCAGCTATGCCACGACGGTTAAC
ATCCATGTGGGCGGGGGAGGGCGGTTGCGGCCAGCCAAGGCCCAGGTCCGGTTGAACCAC
CCTGCTCTCTTGGCCTCCACACAGGAATCTATGGGCCTTCACAGAGCCCAGGGCGCTCCT
GATGCCCCCTTCCACATG
Protein sequence predicted based on nucleotide sequence
MPEGAQGLSLSKPSPSLGCGRRGEVCDCGTVCETRTAPAAPTMASPRGSGSSTSLSTVGS EGDPAPGPTPACSASRPEPLPGPPIRLHLSPVGIPGSARPSRLERVAREIVETERAYVRD LRSIVEDYLGPLLDGGVLGLSVEQVGTLFANIEDIYEFSSELLEDLENSSSAGGIAECFV QRSEDFDIYTLYCMNYPSSLALLRELSLSPPAALWLQERQAQLRHSLPLQSFLLKPVQRI LKYHLLLQELGKHWAEGPGTGGREMVEEAIVSMTAVAWYINDMKRKQEHAARLQEVQRRL GGWTGPELSAFGELVLEGAFRGGGGGGPRLRGGERLLFLFSRMLLVAKRRGLEYTYKGHI FCCNLSVSESPRDPLGFKVSDLTIPKHRHLLQAKNQEEKRLWIHCLQRLFFENHPASIPA KAKQVLLENSLHCAPKSKPVLEPLTPPLGSPRPRDARSFTPGRRNTAPSPGPSVIRRGRR QSEPVKDPYVMFPQNAKPGFKHAGSEGELYPPESQPPVSGSAPPEDLEDAGPPTLDPSGT SITEEILELLNQRGLRDPGPSTHDIPKFPGDSQVPGDSETLTFQALPSRDSSEEEEEEEE GLEMDERGPSPLHVLEGLESSIAAEMPSIPCLTKIPDVPNLPEIPSRCEIPEGSRLPSLS DISDVFEMPCLPAIPSVPNTPSLSSTPTLSCDSWLQGPLQEPAEAPATRRELFSGSNPGK LGEPPSGGKAGPEEDEEGVSFTDFQPQDVTQHQGFPDELAFRSCSEIRSAWQALEQGQLA RPGFPEPLLILEDSDLGGDSGSGKAGAPSSERTASRVRELARLYSERIQQMQRAETRASA NAPRRRPRVLAQPQPSPCLPQEQAEPGLLPAFGHVLVCELAFPLTCAQESVPLGPAVWVQ AAIPLSKQGGSPDGQGLHVSNLPKQDLPGIHVSAATLLPEQGGSRHVQAPAATPLPKQEG PLHLQVPALTTFSDQGHPEIQVPATTPLPEHRSHMVIPAPSTAFCPEQGHCADIHVPTTP ALPKEICSDFTVSVTTPVPKQEGHLDSESPTNIPLTKQGGSRDVQGPDPVCSQPIQPLSW HGSSLDPQGPGDTLPPLPCHLPDLQIPGTSPLPAHGSHLDHRIPANAPLSLSQELPDTQV PATTPLPLPQVLTDIWVQALPTSPKQGSLPDIQGPAAAPPLPEPSLTDTQVQKLTPSLEQ KSLIDAHVPAATPLPERGGSLDIQGLSPTPVQTTMVLSKPGGSLASHVARLESSDLTPPH SPPPSSRQLLGPNAAALSRYLAASYISQSLARRQGPGGGAPAASRGSWSSAPTSRASSPP PQPQPPPPPARRLSYATTVNIHVGGGGRLRPAKAQVRLNHPALLASTQESMGLHRAQGAP DAPFHM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
