Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840891
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3018nts
Open reading frame: 1 to 3018
ATGGAGGGCCAGAGCAGCAGGGGCAGCAGGAGGCCAGGGACCCGGGCTGGCCTGGGTTCC
CTGCCCATGCCCCAGGGTGTTGCCCAAACTGGGGCACCCTCCAAGGTGGACTCAAGTTTT
CAGCTCCCAGCAAAGAAGAACGCAGCCCTAGGACCCTCGGAACCAAGGTTGGCTCTGGCA
CCTGTAGGGCCACGGGCAGCTATGTCAGCTTCCTCGGAAGGACCGAGGCTGGCTCTGGCA
TCTCCCCGACCAATCCTGGCTCCACTGTGTACCCCTGAAGGGCAGAAAACAGCTACTGCC
CACCGCAGCTCCAGCCTGGCCCCAACATCTGTGGGCCAGCTGGTGATGTCTGCCTCAGCT
GGACCAAAGCCTCCCCCAGCGACCACAGGCTCAGTTCTGGCTCCGACGTCCCTGGGGCTG
GTGATGCCTGCCTCAGCAGGGCCAAGATCTCCCCCAGTCACCCTGGGGCCCAATCTGGCC
CCAACCTCCAGGGACCAGAAGCAGGAGCCCCCTGCCTCTGTGGGACCCAAGCCAACACTG
GCAGCCTCTGGCCTGAGCCTGGCCCTGGCTTCTGAGGAGCAGCCCCCAGAACTCCCCTCC
ACCCCTTCCCCGGTGCCCAGTCCAGTTCTGTCTCCAACTCAGGAACAGGCCCTGGCTCCA
GCATCCACGGCATCAGGCGCAGCCTCTGTGGGACAGACATCAGCTAGAAAGAGGGATGCC
CCAGCCCCTAGACCTCTCCCTGCTTCTGAGGGGCATCTCCAGCCTCCAGCTCAGACATCT
GGTCCTACAGGCTCCCCACCCTGCATCCAAACCTCCCCAGACCCTCGGCTCTCCCCCTCC
TTCCGAGCCCGGCCTGAGGCCCTCCACAGCAGCCCTGAGGATCCTGTTTTGCCACGGCCA
CCCCAGACCTTGCCCTTGGATGTGGGCCAGGGTCCTTCAGAGCCTGGCACTCACTCCCCT
GGACTTCTGTCCCCCACCTTCCGGCCTGGGGCCCCCTCAGGCCAGACTGTGCCCCCACCT
CTGCCCAAGCCACCCCGATCACCCAGCCGGTCCCCAAGCCACTCCCCGAATCGCTCTCCC
TGTGTTCCCCCAGCCCCTGACATGGCCCTCCCAAGGCTTGGCACACAGAGTACAGGGCCT
GGAAGGTGCCTGAGCCCCAACCTTCAGGCCCAAGAAGCCCCAGCCCCAGTCACCACCTCC
TCTTCTACATCCACCCTGTCATCCTCCCCTTGGTCAGCTCAGCCTACCTGGAAGAGCGAC
CCCGGCTTCCGGATCACTGTGGTCACATGGAACGTGGGCACTGCCATGCCCCCAGACGAT
GTCACATCCCTCCTCCACCTGGGCGGTGGTGACGACAGCGACGGCGCAGACATGATCGCC
ATAGGGTTGCAGGAAGTGAACTCCATGCTCAACAAGCGACTCAAGGACGCCCTCTTCACG
GACCAGTGGAGTGAGCTGTTCATGGATGCGCTAGGGCCCTTCAACTTCGTGCTGGTGAGT
TCGGTGAGGATGCAGGGTGTCATCCTGCTGCTGTTCGCCAAGTACTACCACCTCCCCTTC
CTGCGAGACGTGCAGACCGACTGCACGCGCACTGGCCTGGGCGGCTACTGGGGTAACAAG
GGTGGCGTGAGCGTGCGCCTGGCGGCCTTCGGGCACATGCTCTGCTTCCTGAACTGCCAC
TTGCCTGCGCATATGGACAAGGCGGAGCAGCGCAAAGACAACTTCCAGACCATCCTCAGC
CTCCAGCAGTTCCAAGGGCCGGGCGCACAGGGCATCCTGGATCATGACCTCGTGTTCTGG
TTCGGGGACCTGAACTTCCGCATTGAGAGCTATGACCTGCACTTTGTCAAGTTTGCCATC
GACAGTGACCAGCTCCATCAGCTCTGGGAGAAGGACCAGCTCAACATGGCCAAGAACACC
TGGCCCATTCTGAAGGGCTTTCAGGAGGGGCCCCTCAACTTCGCTCCCACCTTCAAGTTT
GATGTGGGTACCAACAAATACGATACCAGTGCCAAGAAACGGAAGCCAGCTTGGACAGAC
CGTATCCTATGGAAGGTCAAGGCTCCAGGTGGGGGTCCCAGCCCCTCAGGACGGAAGAGC
CACCGACTCCAGGTGACGCAGCACAGCTACCGCAGCCACATGGAATACACAGTCAGCGAC
CACAAGCCTGTGGCTGCCCAGTTCCTCCTGCAGTTTGCCTTCAGGGACGACATGCCACTG
GTGCGGCTGGAGGTGGCAGATGAGTGGGTGCGGCCCGAGCAGGCGGTGGTGAGGTACCGC
ATGGAAACAGTGTTCGCCCGCAGCTCCTGGGACTGGATCGGCTTATACCGGGTGGGTTTC
CGCCATTGCAAGGACTATGTGGCTTATGTCTGGGCCAAACATGAAGATGTGGATGGGAAT
ACCTACCAGGTAACATTCAGTGAGGAATCACTGCCCAAGGGCCATGGAGACTTCATCCTG
GGCTACTATAGTCACAACCACAGCATCCTCATCGGCATCACTGAACCCTTCCAGATCTCG
CTGCCTTCCTCGGAGTTGGCCAGCAGCAGCACAGACAGCTCAGGCACCAGCTCAGAGGGA
GAGGATGACAGCACACTGGAGCTCCTTGCACCCAAGTCCCGCAGCCCCAGTCCTGGCAAG
TCCAAGCGACACCGCAGCCGCAGCCCGGGACTGGCCAGGTTCCCTGGGCTTGCCCTACGG
CCCTCATCCCGTGAACGCCGTGGTGCCAGCCGTAGCCCCTCACCCCAGAGCCGCCGCCTG
TCCCGAGTGGCTCCTGACAGGAGCAGTAATGGCAGCAGCCGGGGCAGTAGTGAAGAGGGG
CCCTCTGGGCTGCCTGGCCCCTGGGCCTTCCCACCAGCTGTGCCTCGAAGCCTGGGCCTG
TTGCCCGCCTTGCGCCTAGAGACTGTAGACCCTGGTGGTGGTGGCTCCTGGGGACCTGAT
CGGGAGGCCCTGGCGCCCAACAGCCTGTCTCCTAGTCCCCAGGGCCATCGGGGGCTGGAG
GAAGGGGGCCTGGGGCCC
Protein sequence predicted based on nucleotide sequence
MEGQSSRGSRRPGTRAGLGSLPMPQGVAQTGAPSKVDSSFQLPAKKNAALGPSEPRLALA PVGPRAAMSASSEGPRLALASPRPILAPLCTPEGQKTATAHRSSSLAPTSVGQLVMSASA GPKPPPATTGSVLAPTSLGLVMPASAGPRSPPVTLGPNLAPTSRDQKQEPPASVGPKPTL AASGLSLALASEEQPPELPSTPSPVPSPVLSPTQEQALAPASTASGAASVGQTSARKRDA PAPRPLPASEGHLQPPAQTSGPTGSPPCIQTSPDPRLSPSFRARPEALHSSPEDPVLPRP PQTLPLDVGQGPSEPGTHSPGLLSPTFRPGAPSGQTVPPPLPKPPRSPSRSPSHSPNRSP CVPPAPDMALPRLGTQSTGPGRCLSPNLQAQEAPAPVTTSSSTSTLSSSPWSAQPTWKSD PGFRITVVTWNVGTAMPPDDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFT DQWSELFMDALGPFNFVLVSSVRMQGVILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNK GGVSVRLAAFGHMLCFLNCHLPAHMDKAEQRKDNFQTILSLQQFQGPGAQGILDHDLVFW FGDLNFRIESYDLHFVKFAIDSDQLHQLWEKDQLNMAKNTWPILKGFQEGPLNFAPTFKF DVGTNKYDTSAKKRKPAWTDRILWKVKAPGGGPSPSGRKSHRLQVTQHSYRSHMEYTVSD HKPVAAQFLLQFAFRDDMPLVRLEVADEWVRPEQAVVRYRMETVFARSSWDWIGLYRVGF RHCKDYVAYVWAKHEDVDGNTYQVTFSEESLPKGHGDFILGYYSHNHSILIGITEPFQIS LPSSELASSSTDSSGTSSEGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPGLALR PSSRERRGASRSPSPQSRRLSRVAPDRSSNGSSRGSSEEGPSGLPGPWAFPPAVPRSLGL LPALRLETVDPGGGGSWGPDREALAPNSLSPSPQGHRGLEEGGLGP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
