Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840905
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6036nts
Open reading frame: 1 to 6036
ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGACCTA
CACTCCAGCCTCTACTTTGTCAATGCATCTCTGCAAGAGGTAGTGTTTGCCAGCACCACG
GGGACTCTGGTGCCCTGCCCCGCAGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTA
GCCACGGGCGAGGAGATCTACGATGTCCCCGGGATCCGCCACGTCCACCCCAACGGCACT
CTCCAAATTTTCCCCTTCCCTCCTTCAAGCTTCAGTACCTTAATCCATGATAATACTTAT
TATTGCACAGCTGAAAATCCTTCAGGGAAAATTAGAAGTCAGGATGTCCACATCAAGGCT
GTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTT
GCGGTCTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACATCACTGTCGTCTCATGG
GAGAAAGACACTGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCC
TTGTATATTAAAGATGTACAGAATGAAGATGGATTGTATAACTACCGCTGCATCACGCGG
CATCGATACACCGGAGAGACGAGGCAGAGCAACAGCGCCAGACTTTTTGTATCAGACCCA
GCGAACTCAGCCCCATCCATACTGGATGGGTTTGACCATCGCAAAGCCATGGCTGGGCAG
CGTGTGGAGCTGCCTTGCAAAGCGCTCGGGCACCCTGAGCCAGATTACCGCTGGCTGAAG
GACAACATGCCCCTGGAACTTTCAGGGAGGTTCCAGAAGACCGTGACGGGGCTGCTCATT
GAGAACATTCGCCCCTCGGACTCAGGCAGCTATGTTTGTGAAGTGTCCAACAGATACGGA
ACTGCTAAGGTGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCC
AGGAAGGTTAAAAGCAGCGTGGGTAGCCAAGTTTCCTTGTCCTGCAGCGTGACAGGAACT
GAGGACCAGGAACTCTCCTGGTACCGCAATGGTGAAATCCTCAACCCTGGAAAAAATGTG
AGGATCACAGGGATCAACCACGAAAACCTTATAATGGATCACATGGTCAAAAGTGACGGG
GGCGCATACCAGTGCTTTGTGCGCAAGGACAAGCTGTCCGCTCAAGACTATGTGCAGGTC
GTCCTTGAAGATGGAACTCCCAAAATTATTTCTGCCTTTAGTGAAAAGGTGGTGAGTCCA
GCAGAGCCGGTTTCCCTTATGTGCAACGTGAAGGGAACACCTTTGCCCACGATCACGTGG
ACCCTGGACGATGACCCGATTCTCAAGGGTGGCAGTCACCGCATCAGCCAGATGATCACG
TCGGAGGGGAACGTGGTCAGCTACCTGAACATCTCCAGCTCCCAGGTCCGGGACGGGGGA
GTCTACCGCTGCACTGCCAACAACTCGGCGGGAGTCGTCCTGTACCAGGCTCGAATAAAC
GTAAGAGGGCCTGCAAGCATTCGACCAATGAAAAACATCACAGCAATAGCAGGACGGGAC
ACATACATTCACTGTCGTGTGATTGGCTATCCGTATTACTCCATTAAATGGTACAAGAAC
TCTAACCTGCTTCCTTTCAACCACCGCCAAGTGGCATTTGAGAACAATGGAACTCTTAAA
CTTTCAGATGTGCAAAAGGAAGTGGACGAGGGGGAGTACACGTGCAACGTGTTGGTTCAA
CCACAACTCTCCACCAGCCAGAGCGTCCACGTGACCGTGAAAGTTCCGCCTTTCATACAA
CCCTTTGAGTTTCCAAGATTCTCCATTGGGCAGCGGGTCTTCATCCCCTGTGTTGTAGTC
TCAGGGGACTTACCCATCACGATCACCTGGCAGAAGGATGGCCGGCCAATCCCTGGGAGC
CTTGGGGTGACCATTGACAATATTGACTTCACGAGCTCCTTGAGGATTTCCAATCTCTCG
CTCATGCACAATGGGAATTACACGTGCATAGCCCGGAATGAGGCCGCCGCTGTGGAGCAC
CAAAGCCAGTTGATTGTCAGAGTTCCTCCCAAGTTTGTGGTTCAGCCACGGGACCAGGAC
GGGATTTATGGCAAAGCAGTCATCCTCAATTGTTCTGCTGAGGGTTACCCTGTACCTACC
ATCGTGTGGAAATTCTCTAAAGGTGCTGGGGTTCCCCAGTTCCAGCCAATTGCCCTAAAT
GGCCGAATCCAAGTTCTCAGCAATGGGTCGTTGCTGATCAAGCATGTCGTGGAGGAAGAC
AGTGGCTACTACCTCTGCAAGGTCAGCAACGATGTGGGCGCAGACGTCAGCAAGTCCATG
TACCTCACGGTTAAAATTCCTGCGATGATAACATCCTATCCAAATACTACCCTGGCCACG
CAGGGGCAGAAAAAGGAGATGAGCTGCACGGCGCATGGTGAGAAGCCCATTATAGTCCGC
TGGGAGAAGGAGGACCGAATCATTAACCCTGAGATGGCCCGTTATCTTGTGTCCACCAAG
GAGGTGGGAGAAGAGGTGATTTCTACTCTGCAGATTTTGCCAACTGTGAGAGAAGATTCT
GGTTTCTTTTCCTGCCATGCTATTAATTCTTATGGGGAGGACCGTGGAATAATTCAGCTC
ACAGTGCAAGAGCCCCCAGACCCTCCCGAAATTGAGATCAAAGATGTCAAAGCACGCACA
ATTACGCTCAGGTGGACCATGGGGTTTGATGGAAACAGTCCCATCACAGGCTACGATATT
GAATGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAGAGAACCAAAGATGTTTCCCCT
CAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAGCATCCGCATG
TACGCCAAGAACCGGATTGGCAAGAGCGAGCCCAGCAACGAGCTCACCATCACGGCGGAC
GAGGCAGCTCCTGATGGTCCACCTCAGGAAGTTCACCTGGAGCCTATATCATCTCAGAGC
ATCAGGGTCACATGGAAGGCTCCCAAGAAACATTTGCAAAATGGGATTATCCGTGGCTAC
CAAATAGGTTACCGAGAGTACAGCACTGGGGGTAACTTCCAATTCAACATTATCAGTGTC
GACACCAGCGGGGACAGTGAGGTTTACACCCTGGACAACCTGAATAAGTTCACTCAGTAC
GGCCTGGTGGTGCAGGCCTGTAACCGGGCCGGCACGGGGCCTTCTTCTCAGGAAATCATC
ACCACCACTCTCGAGGATGTGCCCAGTTACCCCCCCGAAAATGTCCAAGCCATAGCAACA
TCACCAGAAAGCATATCAATATCCTGGTCCACACTTTCCAAGGAAGCCTTGAATGGAATT
CTCCAGGGGTTCAGAGTCATTTACTGGGCCAACCTCATGGACGGAGAGCTGGGTGAGATT
AAAAACATCACCACCACACAGCCTTCACTGGAGCTGGACGGGCTGGAAAAGTACACCAAC
TACAGCATCCAGGTGCTGGCCTTCACCCGCGCAGGAGACGGGGTCAGGAGTGAGCAGATC
TTCACCCGGACCAAAGAGGATGTTCCAGGTCCTCCCGCGGGTGTGAAGGCAGCGGCGGCC
TCAGCCTCCATGGTCTTTGTGTCCTGGCTTCCCCCTCTCAAGCTGAACGGCATCATCCGA
AAGTACACTGTATTCTGCTCCCACCCCTATCCCACAGTGATCAGCGAGTTTGAGGCCTCT
CCCGACTCGTTTTCCTACAGAATTCCCAACCTGAGTAGGAATCGTCAGTACAGCGTCTGG
GTGGTGGCTGTTACTTCAGCCGGAAGAGGCAACAGCAGTGAAATCATCACAGTCGAGCCA
CTAGCAAAAGCTCCTGCACGAATCCTGACCTTCAGTGGGACAGTGACTACTCCATGGATG
AAAGACATTGTCTTGCCTTGTAAGGCTGTTGGGGACCCTTCTCCTGCAGTCAAATGGATG
AAAGACAGTAACGGGACACCCAGTCTAGTAACGATTGATGGGCGGAGGAGCATCTTTAGC
AACGGAAGCTTCATTATTCGCACGGTGAAAGCAGAAGACTCCGGCTATTACAGCTGCATT
GCCAATAACAACTGGGGATCTGATGAAATTATTTTAAACTTACAAGTACAAGTTCCACCA
GATCAGCCTCGGCTTACAGTCTCCAAGACCACGTCTTCCTCCATCACCCTTTCTTGGCTC
CCTGGAGACAACGGGGGCAGCTCTATCAGAGGATACATACTGCAGTACTCCGAGGACAAT
AGTGAGCAGTGGGGGAGTTTTCCAATCAGCCCCAGCGAACGTTCCTATCGCTTGGAAAAT
CTCAAATGTGGGACTTGGTATAAGTTCACACTGACAGCCCAAAATGGAGTGGGCCCAGGG
CGCATAAGTGAAATCATAGAAGCAAAGACCTTAGGAAAAGAGCCCCAGTTCTCAAAGGAG
CAGGAGCTGTTTGCCAGCATCAACACCACACGCGTGAGGCTGAACCTCATTGGCTGGAAT
GATGGCGGCTGCCCCATCACCTCCTTCACACTAGAGTACAGGCCCTTTGGGACCACAGTT
TGGACCACAGCTCAGAGGACCTCTCTCTCCAAGTCCTACATCCTGTATGACCTGCAGGAA
GCCACCTGGTATGAGCTGCAGATGCGGGTGTGCAACAGTGCGGGCTGCGCGGAGAAGCAG
GCCAACTTCGCTACGCTGAACTACGATGGCAGTACAATTCCTCCACTCATTAAGTCAGTT
GTCCAAAACGAAGAAGGGCTGACGACCAACGAGGGGCTCAAGATGCTGGTGACCATCTCC
TGTATCCTGGTGGGGGTCTTGCTGCTGTTTGTGCTCCTGCTGGTTGTGCGGAGGAGGCGG
CGGGAGCAGAGGCTAAAGAGGCTGCGAGATGCAAAGAGTTTAGCTGAAATGCTCATGAGT
AAGAATACCCGGACTTCAGATACGTTAAGCAAGCAACAGCAGACCCTGCGAATGCACATC
GACATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACGATGGAAACCATTGATGAT
CGCTCCACGGTTCTGTTGACGGATGCTGACTTTGGAGAGGCAGCTAAGCAGAAGTCCCTG
ACGGTCACTCACACGGTCCATTACCAATCGGTGTCTCAGGCCACTGGGCCCTTAGTGGAT
GTTTCAGACGCTCGGCCGGGAACGAATCCCACCACCAGGAGGAATGCCAAGGCTGGGCCC
ACAGCGAGAAACCGCTATGCCAGCCAGTGGACCCTCAACCGACCCCACCCCACCATCTCA
GCACACACCCTCACCACAGACTGGAGGCTGCCAACACCCAGGGCTGCAGGATCAGTAGAC
AAAGAGAGCGACAGTTACAGCGTCAGCCCCTCGCAAGACACAGATCGAGCAAGAAGCAGC
ATGGTCAGCACAGAAAGTGCCTCCTCCACTTACGAAGAACTGGCCAGGGCCTACGAACAC
GCCAAGATGGAAGAGCAACTGAGGCACGCCAAGTTCACCATCACGGAGTGCTTCATATCA
GACACGTCATCGGAGCAGTTGACGGCAGGGACAAATGAGTACACGGACAGTCTGACCTCC
AGCACCCCTTCCGAATCGGGAATCTGCAGGTTCACTGCATCTCCCCCCAAACCTCAGGAT
GGAGGAAGAGTAATGAATATGGCAGTTCCAAAGGCACATCGGCCAGGTGACCTCATACAT
TTGCCTCCATACCTTAGAATGGACTTTTTGTTAAACCGAGGTGGTCCAGGCACCAGCAGG
GACCTGAGCTTAGGACAAGCATGCTTGGAACCTCAGAAAAGCCGGACCCTGAAGCGCCCC
ACGGTCCTGGAGCCCATCCCGATGGAAGCCGCCTCCTCCGCCTCCTCCACGAGAGAAGGA
CAGTCGTGGCAGCCGGGGGCCGTGGCCACATTACCTCAGCGGGAGGGAGCAGAGCTGGGA
CAGGCAGCTAAAATGAGCAGCTCCCAAGAATCACTGCTCGACTCCCGGGGCCATTTGAAA
GGAAACAATCCTTACGCAAAATCTTACACCCTGGTA
Protein sequence predicted based on nucleotide sequence
MWILALSLFQSFANVFSEDLHSSLYFVNASLQEVVFASTTGTLVPCPAAGIPPVTLRWYL ATGEEIYDVPGIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKA VLREPYTVRVEDQKTMRGNVAVFKCIIPSSVEAYITVVSWEKDTVSLVSGSRFLITSTGA LYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVSDPANSAPSILDGFDHRKAMAGQ RVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLIENIRPSDSGSYVCEVSNRYG TAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGTEDQELSWYRNGEILNPGKNV RITGINHENLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSP AEPVSLMCNVKGTPLPTITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGG VYRCTANNSAGVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKN SNLLPFNHRQVAFENNGTLKLSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQ PFEFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLS LMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPT IVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSM YLTVKIPAMITSYPNTTLATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTK EVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKART ITLRWTMGFDGNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRM YAKNRIGKSEPSNELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGY QIGYREYSTGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEII TTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEI KNITTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGPPAGVKAAAA SASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVW VVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWM KDSNGTPSLVTIDGRRSIFSNGSFIIRTVKAEDSGYYSCIANNNWGSDEIILNLQVQVPP DQPRLTVSKTTSSSITLSWLPGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLEN LKCGTWYKFTLTAQNGVGPGRISEIIEAKTLGKEPQFSKEQELFASINTTRVRLNLIGWN DGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQEATWYELQMRVCNSAGCAEKQ ANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLKMLVTISCILVGVLLLFVLLLVVRRRR REQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLLIEERDTMETIDD RSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGP TARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESDSYSVSPSQDTDRARSS MVSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTS STPSESGICRFTASPPKPQDGGRVMNMAVPKAHRPGDLIHLPPYLRMDFLLNRGGPGTSR DLSLGQACLEPQKSRTLKRPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQREGAELG QAAKMSSSQESLLDSRGHLKGNNPYAKSYTLV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
