Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840942
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5820nts
Open reading frame: 1 to 5820
ATGAGTAGTGACACTGAAATGGAAGTGTTCGGCATAGCTGCTCCTTTCCTCCGGAAGTCA
GAAAAGGAGAGGATCGAGGCTCAGAACCAGCCCTTTGATGCCAAGACGTATTGCTTCGTG
GTGGACTCAAAGGAAGAATATGCCAAGGGGAAAATCAAGAGTTCTCAGGATGGGAAGGTC
ACTGTGGAAACTGAGGACAACAGGACCCTGGTGGTCAAACCAGAGGATGTGTACGCCATG
AACCCCCCCAAGTTCGACAGGATCGAAGACATGGCCATGCTGACGCACCTGAATGAGCCA
GCCGTGCTGTACAACCTGAAGGACCGTTACACATCTTGGATGATCTATACCTACTCAGGC
CTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCCGAGGTGGTG
GAAGGCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGAC
AACGCCTATCAGTTCATGCTGACTGATCGTGAAAACCAGTCCATTCTGATCACCGGAGAA
TCCGGGGCAGGAAAGACTGTGAACACCAAACGGGTCATCCAGTACTTTGCAACAATTGCA
GCTACTGGGGACCTGGCCAAGAAGAAGGACTCCAAAATGAAGGGGACTCTGGAAGATCAA
ATCATCAGTGCCAATCCCCTGCTGGAGGCCTTTGGGAACGCCAAGACTGTGAGGAATGAC
AACTCCTCCCGTTTTGGCAAGTTCATCCGAATCCATTTTGGAACCACTGGGAAGCTGGCC
TCTGCAGATATTGAAACTTATCTTCTGGAAAAATCAAGAGTCACTTTCCAGCTGAAGGCT
GAAAGAAGCTACCACATCTTCTACCAGATTCTTTCTAACAAGAAGCCTGAGCTCATAGAG
CTGCTGCTTATTACGACCAACCCTTACGACTACCCGTTCATTAGCCAGGGGGAGATCCTG
GTGGCCAGCATAGATGATGCAGAGGAGCTGCTGGCTACAGACAGCGCCATTGACATCCTG
GGCTTCACCCCAGAAGAGAAATCTGGGCTCTACAAGCTGACGGGAGCCGTGATGCACTAC
GGGAACATGAAGTTCAAGCAGAAGCAGCGAGAGGAGCAGGCCGAGCCGGATGGCACAGAA
GTGGCTGACAAAACAGCCTATCTGATGGGCCTGAACTCTTCGGACCTCCTAAAAGCTTTG
TGCTTTCCTAGAGTGAAAGTTGGGAATGAGTACGTTACCAAAGGTCAAACTGTGGATCAG
GTTCACCATGCTGTGAATGCTCTTTCAAAATCAGTTTATGAAAAGTTGTTCTTGTGGATG
GTCACTCGCATTAACCAGCAACTGGATACGAAGCTTCCAAGACAACACTTCATTGGTGTT
TTGGACATTGCAGGCTTTGAAATCTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAAC
TTCACCAATGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAG
GAGTACAAGAAGGAAGGCATCGAGTGGACGTTCATTGACTTCGGGATGGACCTGGCTGCC
TGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTGGAAGAGGAGTGCATG
TTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCATCTTGGAAAG
TCCAACAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAGGGCCGAGGCTCACTTCTCACTG
ATCCACTATGCGGGCACCGTGGACTACAGCGTCTCAGGTTGGCTGGAGAAGAACAAGGAC
CCTCTGAACGAGACTGTGGTTGGGCTGTACCAGAAGTCTTCCAACAGGCTCCTGGCACAC
CTCTATGCCACGTTTGCCACGGCGGATGCTGACAGTGGAAAGAAGAAAGTTGCCAAGAAG
AAGGGTTCTTCCTTCCAAACTGTCTCTGCCCTTTTCAGGGAAAACCTGAACAAGCTGATG
TCAAATTTAAGAACTACTCACCCTCATTTTGTGCGTTGTATAATTCCCAATGAAACCAAA
ACTCCAGGGGCTATGGAACACAGCCTTGTTCTGCACCAGCTGCGGTGTAACGGTGTCCTG
GAGGGCATCCGCATCTGCAGGAAAGGGTTCCCAAACAGGATTCTCTATGGCGATTTTAAA
CAAAGATACCGAGTGCTGAATGCCAGTGCAATCCCTGAGGGACAATTCATTGACAGCAAG
AAAGCCTGTGAAAAGCTTCTGGCATCCATTGATATTGACCACACTCAGTACAAATTTGGA
CATACCAAGGTGTTCTTCAAGGCTGGCTTGCTGGGAACCCTGGAAGAGATGCGGGATGAC
CGCCTGGCCAAACTAATCACCCGGACACAAGCTGTGTGCAGAGGGTTCCTCATGCGTGTG
GAATTCCAGAAGATGGTGCAGAGGAGGGAGTCCATCTTCTGCATCCAGTACAACATTCGC
TCATTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTC
CTCAAGAGTGCAGAGACTGAGAAAGAGATGGCCACCATGAAGGAAGAATTCCAGAAAACC
AAAGATGAACTCGCCAAGTCGGAGGCAAAAAGGAAGGAGCTAGAGGAAAAACTGGTGACT
CTGGTCCAAGAGAAGAATGACCTGCAGCTCCAAGTACAAGCTGAAAGCGAAAATTTGTTG
GATGCTGAGGAAAGATGCGATCAGCTGATCAAAGCCAAATTCCAGCTCGAGGCCAAGATC
AAGGAGGTGACAGAGAGAGCTGAAGATGAGGAGGAGATCAATGCTGAGCTGACGGCCAAG
AAGAGGAAACTGGAGGATGAATGCTCAGAGCTCAAGAAAGACATTGATGACCTTGAGTTG
ACCCTGGCCAAGGTTGAGAAGGAGAAGCATGCCACAGAGAACAAGGTTAAAAACCTTACT
GAGGAACTCTCTGGGTTAGATGAAACAATTGCAAAGTTAACCAGAGAGAAGAAGGCCCTC
CAAGAGGCGCACCAGCAGGCCTTGGATGACCTCCAAGCTGAAGAAGACAAAGTCAATTCT
TTGAACAAAACCAAGAGCAAACTGGAACAGCAAGTGGAAGACCTGGAAAGCTCCCTAGAA
CAAGAAAAGAAGCTCCGAGTAGACCTGGAAAGGAACAAAAGGAAATTGGAAGGAGACTTG
AAGCTTGCTCAAGAGTCCATATTAGATCTGGAGAATGACAAGCAACAGCTGGACGAAAGG
CTCAAGAAGAAAGATTTTGAATATTGTCAACTTCAAAGCAAAGTGGAAGATGAGCAGACA
CTGGGCCTCCAGTTTCAGAAGAAAATCAAAGAGTTGCAGGCTCGAATTGAGGAGCTGGAA
GAGGAGATAGAGGCGGAGAGGGCCACCCGCGCGAAGACAGAGAAACAGCGCAGCGACTAT
GCCCGGGAGCTGGAGGAGCTGAGCGAGCGGCTGGAGGAGGCGGGAGGCGTCACCTCCACG
CAGATAGAGCTCAACAAGAAGCGGGAGGCGGAGTTCCTGAAGCTGCGCAGGGACCTGGAG
GAGGCCACACTGCAGCACGAAGCCATGGTGGCCGCGCTGAGGAAGAAGCATGCGGATAGT
GTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGGGTCAAGCAGAAGCTGGAGAAG
GAGAAGAGCGAGTTCAAGCTGGAGATCGATGACCTCTCCAGCAGCATGGAGAGTGTGTCG
AAATCTAAGGCAAATCTGGAAAAAATCTGCCGAACCCTGGAGGATCAGTTAAGTGAGGCC
AGGGGCAAGAATGAGGAAATTCAGAGGAGCCTGAGCGAGCTGACCACACAGAAGTCTCGT
TTGCAGACCGAGGCTGGTGAGCTGAGTCGTCAGCTGGAAGAAAAAGAAAGCATAGTATCC
CAACTTTCCAGGAGCAAGCAAGCCTTTACCCAGCAAACAGAAGAGCTCAAGAGGCAGCTG
GAGGAAGAGAACAAGGCCAAGAACGCCCTGGCGCACGCCCTGCAGTCCTCCCGCCACGAC
TGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAGGCAAAGCTGAGCTGCAGAGG
GCGCTGTCCAAGGCCAATAGTGAGGTTGCCCAGTGGAGAACCAAATACGAGACGGACGCC
ATCCAGCGCACAGAAGAGCTGGAGGAGGCCAAGAAAAAACTTGCTCAGCGCCTTCAAGAT
TCCGAGGAACAGGTTGAGGCAGTGAATGCTAAATGTGCTTCACTGGAGAAGACCAAGCAG
AGGCTGCAAGGAGAGGTGGAGGATCTGATGGTTGATGTTGAAAGAGCCAATTCCTTGGCC
GCCGCTCTGGACAAGAAGCAGAGGAACTTTGACAAGGTGTTGGCAGAGTGGAAGACAAAG
TGTGAGGAGAGCCAAGCAGAGCTGGAGGCATCCCTGAAGGAGTCCCGCTCCTTGAGCACT
GAGCTCTTCAAACTGAAAAATGCCTACGAGGAAGCCTTAGATCAACTTGAAACTGTGAAA
CGGGAAAATAAGAACTTAGAGCAGGAGATAGCAGATCTCACAGAACAAATTGCTGAAAAT
GGCAAAACCATCCATGAACTGGAGAAATCAAGAAAGCAGATTGAGCTGGAAAAGGCTGAT
ATCCAGCTGGCTCTCGAGGAAGCAGAGGCTGCTCTTGAGCATGAAGAAGCCAAGATCCTC
CGAATCCAGCTTGAATTGACACAAGTGAAATCAGAAATTGATAGAAAGATCGCCGAGAAG
GATGAAGAGATCGAGCAGCTGAAGAGGAACTACCAGAGAACAGTGGAAACCATGCAGAGC
GCCCTGGACGCCGAGGTGCGGAGCAGGAATGAAGCCATCCGGCTCAAGAAGAAGATGGAG
GGGGACCTGAATGAAATCGAGATCCAGCTGAGCCACGCCAACCGCCAGGCGGCGGAGACA
CTCAAACACCTCAGGAGTGTCCAGGGACAGCTGAAGGATACGCAGCTCCACCTGGATGAT
GCCCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCGATTGTGGAGCGCAGAGCCAAC
CTGCTGCAGGCCGAGGTGGAGGAGCTGCGGGCTACTCTGGAGCAGACGGAGAGGGCCCGG
AAACTGGCGGAACAGGAGCTCCTGGACTCCAACGAGAGGGTGCAGCTGCTGCATACCCAG
AACACCAGCCTCATCCACACCAAGAAGAAGCTGGAGACAGACCTCATGCAGCTCCAGAGT
GAGGTAGAAGATGCCAGCAGGGATGCAAGGAACGCTGAGGAGAAGGCCAAGAAGGCCATC
ACGGACGCTGCCATGATGGCGGAGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCACCTT
GAGCGGATGAAGAAGAACCTGGAACAGACGGTGAAGGACCTGCAGCATCGTCTAGATGAG
GCCGAGCAGCTGGCGCTGAAGGGCGGGAAGAAGCAGATCCAGAAACTGGAAACCAGGATC
CGAGAGCTGGAGTTTGAACTTGAGGGAGAGCAGAAGAAGAACACAGAGTCTGTTAAGGGC
CTGAGGAAGTATGAGCGGAGGGTCAAGGAGCTGACGTACCAGAGTGAAGAGGACAGGAAG
AATGTGCTGAGATTGCAGGATCTGGTGGATAAACTGCAAGTGAAAGTCAAGTCCTACAAG
AGGCAGGCGGAGGAGGCTGATGAACAAGCCAATGCTCATCTCACCAAATTCCGAAAGGCT
CAGCATGAGCTGGAGGAGGCCGAGGAACGTGCGGATATCGCAGAATCTCAAGTCAACAAG
CTCCGCGCTAAGACTCGAGACTTCACCTCCAGCAGGATGGTGGTCCACGAGAGTGAAGAG
Protein sequence predicted based on nucleotide sequence
MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKV TVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSG LFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGE SGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRND NSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIE LLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHY GNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQ VHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCIN FTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECM FPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKD PLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLM SNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFK QRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDD RLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPL LKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLL DAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLEL TLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNS LNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDER LKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDY ARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADS VAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEA RGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQL EEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDA IQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLA AALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVK RENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKIL RIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKME GDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRAN LLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQS EVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDE AEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRK NVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNK LRAKTRDFTSSRMVVHESEE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
