Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840965
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4611nts
Open reading frame: 1 to 4611
ATGGGGGAGAAGCTGGAGCTGAGACTGAAGTCGCCCGTGGGGGCTGAGCCCGCCGTCTAC
CCGTGGCCGCTGCCGGTCTACGATAAACATCACGATGCCGCTCATGAAATCATCGAAACC
ATCCGATGGGTCTGTGAAGAAATCCCGGATCTCAAGCTCGCTATGGAGAATTACGTTTTA
ATTGACTATGACACCAAAAGTTTCGAGAGCATGCAGAGGCTCTGCGACAAGTACAACCGT
GCCATCGACAGCATCCACCAGCTGTGGAAGGGCACCACGCAGCCTATGAAGCTGAACACG
CGGCCGTCCACTGGACTCCTCCGCCATATCCTGCAGCAGGTCTACAACCACTCGGTGACC
GACCCCGAGAAGCTCAACAACTACGAGCCCTTCTCCCCCGAGGTGTACGGCGAGACATCC
TTCGACCTGGTGGCCCAGATGATTGATGAGATCAAGATGACCGACGACGACCTGTTTGTG
GACTTGGGGAGCGGTGTGGGCCAGGTCGTGCTCCAGGTTGCTGCTGCCACCAACTGCAAA
CATCACTATGGCGTCGAGAAAGCAGACATCCCGGCCAAGTATGCGGAGACAATGGACCGC
GAGTTCAGGAAGTGGATGAAATGGTATGGAAAAAAGCATGCAGAATACACATTGGAGAGA
GGCGATTTCCTCTCAGAAGAGTGGAGGGAGCGAATCGCCAACACCAGTGTTATATTTGTG
AATAATTTTGCCTTTGGTCCTGAGGTGGATCACCAGCTGAAGGAGCGGTTTGCAAACATG
AAGGAAGGTGGCAGAATCGTGTCCTCGAAACCCTTTGCACCTCTGAACTTCAGAATAAAC
AGTAGAAACTTGAGTGACATCGGCACCATCATGCGCGTGGTGGAGCTCTCGCCCCTGAAG
GGCTCGGTGTCGTGGACGGGGAAGCCAGTCTCCTACTACCTGCACACTATCGACCGCACC
ATACTTGAAAACTATTTTTCTAGTCTGAAAAACCCAAAACTCAGGGAGGAACAGGAGGCC
GCTCGGCGACGACAGCAGCGCGAAAGCAAGAGCAACGCGGCCACGCCCACTAAGGGCCCA
GAGGGCAAAGTGGCCGGACCTGCCGACGCCCCCATGGACTCTGGTGCTGAGGAAGAGAAG
GCGGGAGCAGCCACCGTGAAGAAGCCGTCTCCCTCCAAAGCCCGCAAGAAGAAGCTAAAC
AAGAAGGGGAGGAAGATGGCTGGCCGCAAGCGCGGGCGGCCCAAGAAGATGAACACTGCG
AACCCCGAGCGGAAGCCCAAGAAGAACCAAACTGCACTGGATGCCCTGCACGCTCAGACC
GTGTCTCAGACGGCGGCCTCCTCACCCCAGGATGCCTACAGATCCCCTCACAGCCCGTTC
TACCAGCTACCTCCGAGCGTGCAGCGGCACTCCCCCAACCCGCTGCTGGTGGCGCCCACC
CCGCCCGCGCTGCAGAAGCTTCTAGAGTCCTTCAAGATCCAGTACCTCCAGTTCCTGGCA
TACACAAAGACCCCCCAGTACAAGGCCAGCCTGCAGGAGCTGCTTGGCCAGGAGAAGGAG
AAGAACGCCCAGCTGCTGGGTGCGGCTCAGCAGCTCCTCAGCCACTGCCAGGCCCAGAAG
GAGGAGATCAGGAGGCTGTTTCAGCAAAAATTGGATGAGCTTGGTGTGAAGGCGCTCACC
TACAACGACCTGATTCAAGCGCAGAAGGAGATCTCCGCCCATAACCAGCAGCTCCGGGAG
CAGTCCGAGCAGCTGGAGCAGGACAACCGCGCGCTCCGCGGCCAGAGCTTGCAGCTGCTC
AAGGCTCGCTGCGAGGAGCTGCAGCTGGACTGGGCCACGCTGTCGCTCGAGAAGCTGTTG
AAGGAGAAGCAGGCCCTGAAGAGCCAGATCTCGGAGAAGCAGAGGCACTGCCTGGAGCTG
CAGATCAGCATTGTGGAGCTAGAGAAGAGCCAGCGGCAGCAGGAGCTCCTGCAGCTCAAG
TCCTGTGTGCCGCCTGACGACGCCCTGTCCCTGCACCTTCGTGGGAAGGGCGCCCTGGGC
CGCGAGCTTGAGCCTGACGCCAGTCGGCTGCACCTGGAGCTGGACTGCACCAAGTTCTCG
CTGCCTCACTTGAGCAGCATGAGCCCGGAGCTCTCCATGAACGGCCAGGCTGCTGGCTAT
GAGCTCTGCGGTGTGCTGAGCCGGCCTTCGTCGAAGCAGAACACGCCCCAGTACCTGGCC
TCACCCCTGGACCAGGAGGTGGTGCCTTGTACACCTAGCCACGTCGGCCGGCCCCGCCTG
GAGAAGCTGTCCGGCCTGGCCGCACCCGACTACACTAGGCTGTCCCCGGCCAAGATTGTG
CTCAGGCGGCACCTGAGCCAGGACCACACAGTGCCCGGCCGGCCGGCTGCCAGTGAGCTC
CATTCGAGAGCCGAGCACACCAAGGAGAACGGCCTTCCCTACCAGAGCCCCAGCGTGCCT
GGCAGCATGAAGCTGAGCCCTCAGGACCCGCGGCCTCTGTCCCCTGGGGCCTTGCAGCTT
GCTGGAGAGAAGAGCAGCGAGAAGGGCCTGAGAGAGCGCGCCTACGGCAGCAGCGGGGAG
CTCATCACCAGCCTGCCCATCAGTATCCCGCTCAGCACCGTGCAGCCCAACAAGCTCCCG
GTCAGCATTCCCCTGGCCAGCGTCGTGCTGCCCAGTCGCGCCGAGAGGGCGAGGAGCACC
CCCAGTCCTGTGCTGCAGCCTCGTGACCCCTCGTCCACACTGGAAAAGCAGATTGGCGCT
AATGCCCACGGTGCTGGGAGCAGAAGCCTTGCCCTGGCCCCCGCAGGATTCTCCTATGCT
GGCTCTGTGGCCATCAGCGGGGCCCTGGCCGGCAGCCCGGCCTCTCTCACACCTGGTGCC
GAGCCGGCTACCTTGGATGAGTCCTCCAGTTCTGGGAGCCTTTTTGCCACCGTGGGGTCC
CGCAGCTCCACACCCCAGCACCCCCTGTTGCTGGCACAGCCCCGGAATTCGCTTCCTGCC
TCTCCCGCCCACCAGCTCTCCTCCAGCCCCCGGCTTGGTGGGGCCGCTCAGGGACCGTTG
CCCGAGGCTAGCAAGGGAGATCTGCCCTCCGATTCCGGCTTCTCAGATCCTGAGAGTGAA
GCTAAGAGGAGGATTGTGTTCACCATCACCACTGGTGCCGGCAGTGCCAAGCAGTCGCCC
TCCAGCAAGCACAGCCCCCTGACCGCCAGCGCCCGTGGGGACTGTGTTCCGAGTCACGGA
CAGGACAGTCGCAGGCGCGGCAGGCGGAAGCGAGCATCTGCTGGGACGCCCAGCTTAAGC
GCAGGCGTGTCCCCCAAGCGCAGAGCCCTCCCGTCCGTCGCTGGCCTTTTCACACAGCCT
TCGGGGTCCCCCCTCAACCTCAACTCCATGGTCAGTAACATCAACCAGCCCCTGGAGATT
ACAGCCATCTCGTCCCCGGAGACATCCCTGAAAAGCTCCCCTGTGCCCTACCAGGACCAC
GACCAGCCCCCCGTGCTTAAGAAGGAGCGGCCTCTGAGCCAGACCAATGGGGCACACTAC
TCCCCACTCACCTCAGACGAGGAGCCAGGCTCTGAGGACGAGCCCAGCAGTGCTCGAATT
GAGAGAAAAATTGCAACAATCTCCTTAGAAAGCAAATCTCCCCCGAAAACCTTGGAAAAT
GGTGGTGGCTTGGCGGGAAGGAAGCCCGCGCCCGCCGGCGAGCCAGTCAATAGCAGCAAG
TGGAAGTCCACCTTCTCGCCCATTTCCGACATCGGCCTGGCTAAGTCGGCGGACAGCCCG
CTGCAGGCCAGCTCCGCCCTCAGCCAGAACTCCCTGTTCACGTTCCGGCCCGCCCTGGAG
GAGCCCTCTGCCGATGCCAAGCTGGCCGCTCACCCTAGGAAGGGCTTTCCCGGCTCCCTG
TCGGGGGCTGATGGACTCAGCCCGGGCACCAACCCTGCCAACGGCTGCACCTTCGGCGGG
GGACTGGCCGCGGACCTGAGTTTACACAGCTTCAGTGATGGTGCTTCTCTTCCCCACAAG
GGCCCCGAGGCGGCCGGCCTGAGCTCCCCGCTGAGCTTCCCCTCGCAGCGCGGCAAGGAG
GGCTCGGACGCCAACCCTTTCCTGAGCAAGAGGCAGCTTGACGGCCTGGCTGGGCTGAAG
GGCGAGGGCAGCCGAGGCAAGGAGGCAGGGGAAGGCGGCCTGCCTCTGTGCGGGCCAACG
GACAAGACCCCACTGCTGAGCGGCAAGGCCGCCAAAGCCCGGGACCGCGAGGTGGACCTC
AAGAATGGCCACAACCTCTTCATCTCTGCGGCGGCCGTCCCTCCTGGAAGCCTCCTCAGC
GGCCCCGGCCTGGCCCCGGCTGCATCTTCCGCAGGCGGAGCGGCTTCCTCTGCTCAAACG
CATAGGTCTTTCCTGGGCCCCTTCCCGCCTGGCCCGCAGTTCGCGCTCGGCCCCATGTCC
CTGCAGGCTAACCTCGGATCCGTGGCCGGCTCTTCCGTGCTGCAGTCGCTCTTCAGCTCC
GTGCCCGCCGCCGCAGGCCTGGTGCACGTGTCCTCCGCTGCCACCAGGCTGACCAACTCG
CACGCCATGGGCAGTTTTTCCGGGGTGGCAGGCGGCACAGTTGGAGGTAAC
Protein sequence predicted based on nucleotide sequence
MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVL IDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVT DPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFVDLGSGVGQVVLQVAAATNCK HHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFV NNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLK GSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGP EGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTA NPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLPPSVQRHSPNPLLVAPT PPALQKLLESFKIQYLQFLAYTKTPQYKASLQELLGQEKEKNAQLLGAAQQLLSHCQAQK EEIRRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEQDNRALRGQSLQLL KARCEELQLDWATLSLEKLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLK SCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTKFSLPHLSSMSPELSMNGQAAGY ELCGVLSRPSSKQNTPQYLASPLDQEVVPCTPSHVGRPRLEKLSGLAAPDYTRLSPAKIV LRRHLSQDHTVPGRPAASELHSRAEHTKENGLPYQSPSVPGSMKLSPQDPRPLSPGALQL AGEKSSEKGLRERAYGSSGELITSLPISIPLSTVQPNKLPVSIPLASVVLPSRAERARST PSPVLQPRDPSSTLEKQIGANAHGAGSRSLALAPAGFSYAGSVAISGALAGSPASLTPGA EPATLDESSSSGSLFATVGSRSSTPQHPLLLAQPRNSLPASPAHQLSSSPRLGGAAQGPL PEASKGDLPSDSGFSDPESEAKRRIVFTITTGAGSAKQSPSSKHSPLTASARGDCVPSHG QDSRRRGRRKRASAGTPSLSAGVSPKRRALPSVAGLFTQPSGSPLNLNSMVSNINQPLEI TAISSPETSLKSSPVPYQDHDQPPVLKKERPLSQTNGAHYSPLTSDEEPGSEDEPSSARI ERKIATISLESKSPPKTLENGGGLAGRKPAPAGEPVNSSKWKSTFSPISDIGLAKSADSP LQASSALSQNSLFTFRPALEEPSADAKLAAHPRKGFPGSLSGADGLSPGTNPANGCTFGG GLAADLSLHSFSDGASLPHKGPEAAGLSSPLSFPSQRGKEGSDANPFLSKRQLDGLAGLK GEGSRGKEAGEGGLPLCGPTDKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLS GPGLAPAASSAGGAASSAQTHRSFLGPFPPGPQFALGPMSLQANLGSVAGSSVLQSLFSS VPAAAGLVHVSSAATRLTNSHAMGSFSGVAGGTVGGN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
