Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840969
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3519nts
Open reading frame: 1 to 3519
ATGGTCACAGTGGGGACCAATATCCTGCCCTCCAGACCCGCTGCTTCTGCCAATACAGCA
AGGGAAGACGCAGCTCTGTTTTCTAGAAGGATCCCACCAAGGCATAAAAACGGCGCAGCC
CAGCCTGGAGCTGCTCCTGGTCCTGGAGCCCCGGGCGCTAACATGGGGAATGCGCACTCC
AAAAGCGGGGACAGGCACAGCGCGCTCCCCGGCCGACCCGAGCTGTCTTTTTACGGCTCT
TTCCCCAGGAAATGGAGCGAGAACGTCTTCTTGGATAACGAGCTCCTGACCTCTAAGATC
CTGTCTGTGCTGCGGCCGCAGTCGGAGCGGGGCTTCCGCGCCGGGGACCTCCGCTACCCG
ACCCACTTTCTCAGCACCAACTCTGTCCTTGCCTCTGTCACAGCCTCGCTCAAGGAGCAC
CCCCGGGGCACCCTCCTCTCCGATGGCAGCCCGGCCCTGTCCAGGAATGTCGGTATGACA
GTCTCTCAGAAAGGGGGTCCCCAGCCAACACCCAGCCCCGCTGGCCCTGGGACGCAACTC
GGACCAATCACAGGAGAGATGGATGAAGCCGATTCCGCGTTTTTAAAATTTAAGCAAACA
GCTGATGACTCTCTGTCCCTTACATCTCCAAACACCGAGTCCATTTTTGTAGAAGATCCC
TACACCGCCTCGCTGAGGAGCGAGATTGAGTCAGACGGCCACGAGTTTGAAGCTGAATCC
TGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGAAGCAAAAGAGGGAGGTGGTGAAAAGA
CAAGACGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGTGCGGACGCTCAAGATC
ATGCTGAAGGTGTACTCCAGGGCTCTGCAGGAGGAGCTGCAGTTCAGCAGCAAGGCCATT
GGCCGCCTGTTCCCATGCGCTGACGACCTGTTGGAGACGCACAGCCACTTCCTCGCTCGG
CTCAAGGAGCGCCGCCAGGAATCCCTGGAGGAGGGCAGTGACCGGAATTATGTGATCCAG
AAAATCGGGGACCTCCTGGTTCAGCAGTTTTCAGGTGAAAATGGGGAGAGAATGAAAGAA
AAGTACGGTGTGTTTTGTAGTGGACACAATGAAGCTGTTAGTCATTACAAGTTGCTGCTG
CAGCAAAACAAGAAATTTCAAAACTTGATCAAGAAAATTGGCAACTTCTCCATCGTGAGG
AGGCTGGGCGTGCAAGAGTGCATTCTCCTCGTTACACAACGCATAACCAAATACCCAGTG
CTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAAGACCTCACC
CAGGCCTTGAACCTCATCAAAGATATCATCTCACAAGTGGACGCTAAGGTCAGTGAGTGT
GAGAAGGGCCAGCGGCTCAGGGAGATCGCCGGGAAGATGGACCTGAAGTCTTCCAGCAAA
CTCAAGAACGGGCTTACCTTCCGCAAGGAAGACATGCTTCAGCGGCAGCTGCACCTGGAG
GGCATGCTATGCTGGAAGACCACATCAGGCCGCTTGAAAGATATCCTGGCAATCCTGCTG
ACCGACGTACTTTTGCTGCTACAAGAAAAAGATCAGAAATACGTCTTTGCCTCAGTGGAC
TCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATCGTGAGGGAAGTGGCCAACGAGGAG
AAAGCGATGTTTCTTATCAGCGCTTCCTTGCAAGGGCCGGAGATGTATGAAATCTACACG
AGCTCCAAAGAGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAGAGCTGC
CCTGACGAGGAGGAAGGCCCCTTCTCCCTGCCCGAAGAGGAAAGGAAGGTGGTCGAGGCC
CGCGCCACGAGATTGCGGGACTTTCAAGAGCGGTTGAGCATGAAGGACCAGCTGATCGCA
CAGAGCCTCCTAGAGAAACAGCAGATATACCTGGAGATGGCCGAGATGGGCGGCCTCGAA
GACCTGCCCCAGCCCCGAGGCCTATTCCGTGGAGGGGACCCATCTGAGACTCTGCAGGGC
GAGCTAATTCTCAAGTCGGCCATGAGCGAGATCGAGGGCATCCAGAGCCTTATCTGCAGG
CAGCTGGGCAGCGCTAACGGCCAGGCGGAAGACGGAGGCAGCTCTACAGGCCCGCCCAGG
AGGGCTGAGACATTCGCGGGCTACGACTGTACAAACAGCCCCACCAAGAATGGCAGTTTC
AAGAAGAAAGTCAGCAGCACTGACCCCAGGCCTCGAGACTGGCGAGGCCCCCCAAACAGC
CCCGACTTGAAGCTCAGCGACAGTGACATTCCTGGGAGCTCTGAGGAATCGCCGCAAGTG
GTGGAGGCGCCAGGAACGGAATCCGATCCCCGTCTGCCCACCGTCCTGGAGTCGGAGCTT
GTCCAGCGGATTCAGACACTGTCCCAGCTGCTCCTGAACCTTCAGGCGGTAATCGCCCAC
CAGGACAGCTATGTGGAGACGCAGCGGGCTGCCATCCAGGAGCGGGAGAAGCAGTTCCGG
CTGCAGTCGACGCGGGGGAACCTGCTGCTGGAGCAGGAGCGGCAACGCAACTTCGAGAAG
CAGCGGGAGGAGCGCGCGGCCCTCGAGAAGCTGCAGAGCCAGCTGCGGCACGAGCAGCAA
CGCTGGGAGCGCGAGCGCCAGTGGCAGCATCAGGAGCTTGAGCGTGCTGGCGCGCGGCTA
CAAGAACGCGAGGGCGAGGCGAGGCAGTTACGCGAGCGGCTGGAGCAGGAACGGGCCGAG
CTGGAGCGCCAGCGACAAGCTTACCAACACGACCTGGAGCGGCTGCGCGAGGCTCAGCGT
GCCGTGGAACGCGAGCGGGAGCGCCTGGAGTTACTGCGACGCCTCAAGAAGCAGAACACC
GCGCCAGGCGCACTGCCGCCCGACACACTGGCTGAGGCTCAGCCCCCAAGCCACCCTCCC
AGCTTCAACGGGGAAGGGCTGGAGGGACCTCGTGTGAGCATGCTGCCATCCGGCGTGGGA
CCAGAGTACGCAGAGCGCCCTGAGGTTGCTCGCCGGGACAGCGCCCCCACCGAGAACCGG
CTGGCCAAGAGCGATGTGCCCATCCAGCTGCTCAGCGCCACCAACCAGTTCCAGAGGCAG
GCGGCCGTGCAGCAGCAGATCCCCACCAAGCTGGCGGCTTCTACCAAGGGTGGTAAGGAC
AAGGGCGGCAAGAGTAGGGGCTCTCAGCGGTGGGAGAGCTCCGCGTCCTTCGACCTGAAG
CAACAGCTGCTGCTCAACAAGCTCATGGGGAAAGATGAGAGCACCTCACGGAACCGCCGC
TCGCTGAGCCCTATCCTTCCCGGCAGGCACAGTCCTGCGCCTCCACCTGACCCTGGATTT
CCCGCACCGAGCCCTCCGCCAGCTGACAGCCCCTCTGAGGGATTCTCTCTCAAGGCTGGG
GGCACAGCCCTCCTGCCCGGGCCCCCAGCTCCCTCTCCATTGCCGGCCACACCACTCAGC
GCCAAGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTC
Protein sequence predicted based on nucleotide sequence
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHS KSGDRHSALPGRPELSFYGSFPRKWSENVFLDNELLTSKILSVLRPQSERGFRAGDLRYP THFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAES WSLAVDAAYAKKQKREVVKRQDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSKAI GRLFPCADDLLETHSHFLARLKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKE KYGVFCSGHNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPV LVERIIQNTEAGTEDYEDLTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSK LKNGLTFRKEDMLQRQLHLEGMLCWKTTSGRLKDILAILLTDVLLLLQEKDQKYVFASVD SKPPVISLQKLIVREVANEEKAMFLISASLQGPEMYEIYTSSKEDRNAWMAHIQRAVESC PDEEEGPFSLPEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQIYLEMAEMGGLE DLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICRQLGSANGQAEDGGSSTGPPR RAETFAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQV VEAPGTESDPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFR LQSTRGNLLLEQERQRNFEKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARL QEREGEARQLRERLEQERAELERQRQAYQHDLERLREAQRAVERERERLELLRRLKKQNT APGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSGVGPEYAERPEVARRDSAPTENR LAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGSQRWESSASFDLK QQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADSPSEGFSLKAG GTALLPGPPAPSPLPATPLSAKEDASKEDVIFF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
