Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00841047
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5265nts
Open reading frame: 1 to 5265
ATGGCGTCGGAGCTAGAGCCCGAGGTGCAGGCCATCGACCGCAGCTTGCTCGAATGTTCG
GCCGAGGAGATTGCGGGGAAATGGCTGCAAGCAACTGACCTCACTAGGGAAGTGTACCAG
CATTTAGCCCACTATGTACCCAAAATCTACTGCAGGGGTCCCAACCCTTTTCCACAGAAA
GAAGACATGCTGGCACAGCATGTTTTGTTGGGACCAATGGAATGGTACTTATGTGGTGAA
GATCCAGCATTTGGATTTCCAAAACTTGAGCAAGCAAACAAACCTTCCCATCTTTGTGGT
CGTGTTTTTAAAGTAGGAGAGCCCACATATTCTTGCAGAGACTGTGCAGTTGATCCAACT
TGTGTTTTGTGCATGGAGTGCTTTTTGGGAAGCATTCACAGAGATCATCGATATAGGATG
ACAACATCAGGAGGTGGAGGTTTCTGTGACTGTGGTGATACTGAAGCCTGGAAAGAGGGT
CCTTACTGTCAAAAACATGAACTTAACACCTCTGAAATTGAGGAAGAAGAGGATCCTCTT
GTTCATTTATCAGAAGATGTGATAGCAAGAACCTATAACATTTTTGCCATTACGTTTCGG
TATGCAGTAGAAATATTAACCTGGGAAAAAGAAAGTGAATTGCCAGCAGATTTAGAGATG
GTAGAGAAGAGTGACACCTACTATTGCATGCTGTTTAATGATGAGGTTCACACATATGAA
CAAGTTATTTATACTCTTCAGAAAGCCGTTAACTGTACACAAAAAGAAGCTATTGGTTTT
GCAACTACCGTAGATCGAGATGGGCGCAGGTCTGTTCGATATGGAGATTTTCAGTATTGT
GAGCAAGCAAAATCAGTAATTGTGAGAAATACCAGTAGACAAACAAAGCCACTCAAAGTT
CAAGTTATGCATTCGTCGATCGTCGCACATCAGAATTTTGGTTTGAAACTTTTGTCTTGG
CTGGGAAGTATTATTGGATATTCAGATGGCCTTCGCCGGATTTTATGTCAAGTTGGTTTA
CAAGAAGGGCCAGATGGTGAAAACTCCTCTCTAGTGGACAGACTTATGCTTAGCGATTCC
AAACTATGGAAAGGCGCTAGAAGTGTATATCATCAGTTGTTCATGAGCAGTCTGCTTATG
GATTTGAAATACAAGAAACTATTTGCTGTTCGATTTGCAAAAAATTACCAGCAGTTGCAG
AGAGATTTTATGGAGGATGATCACGAGAGAGCAGTGTCGGTGACTGCTCTATCTGTCCAG
TTCTTCACCGCACCTACTCTGGCTCGAATGCTCATCACAGAAGAAAACTTAATGAGCATC
ATCATTAAGACTTTTATGGATCATTTGAGACATCGAGATGCCCAGGGCAGATTTCAGTTT
GAACGATACACTGCTTTACAAGCCTTCAAATTTAGGAGAGTACAGAGCCTTATTTTAGAT
CTCAAGTATGTGTTAATTAGCAAACCAACTGAATGGTCAGATGAGCTGAGGCAGAAGTTC
CTAGAAGGGTTTGATGCCTTTTTAGAATTACTAAAATGTATGCAGGGAATGGATCCAATT
ACACGTCAAGTAGGACAACATATTGAAATGGAACCAGAGTGGGAAGCAGCCTTCACACTT
CAAATGAAATTAACACATGTCATTTCAATGATGCAGGACTGGTGTGCTTCAGATGAAAAA
GTGTTAATCGAAGCTTACAAGAAATGTCTCGCTGTACTCATGCAGTGTCATGGTGGCTAT
ACTGATGGTGAACAGCCTATCACACTAAGTATTTGTGGCCATTCAGTGGAAACTATCAGG
TACTGCGTTTCCCAAGAAAAAGTGAGCATTCACCTCCCAGTTTCTCGGTTACTGGCAGGT
TTACATGTATTATTAAGCAAAAGTGAAGTAGCATATAAATTTCCAGAGCTCCTACCTCTC
AGTGAACTTAGCCCACCCATGTTGATAGAACACCCTCTGAGATGTCTTGTCCTGTGTGCC
CAGGTACATGCGGGAATGTGGAGAAGGAATGGGTTCTCTCTAGTAAACCAGATTTATTAC
TACCATAATGTGAAATGCAGACGTGAGATGTTTGACAAGGATGTAGTGATGCTTCAGACA
GGTGTCTCCATGATGGATCCAAATCATTTCCTGATGATCATGCTCAGCCGCTTTGAACTT
TATCAGATTTTCTCTACCCCAGACTATGGAAAAAGATTCAGTTCTGAGATTACCCATAAG
GATGTTGTTCAGCAGAACAATACTCTAATAGAAGAAATGCTATACCTCATTATCATGCTT
GTGGGAGAGAGATTTAGTCCTGGAGTTGGACAGGTAAATGCTACAGATGAAATCAAGCGA
GAGATTATCCATCAGTTGAGTATCAAGCCTATGGCTCATAGTGAATTGGTAAAATCTTTG
CCTGAAGATGAGAACAAGGAGACTGGCATGGAGAGTGTAATCGAAGCGGTTGCCCATTTC
AAGAAACCTGGATTAACAGGACGAGGCATGTATGAACTGAAACCAGAATGTGCCAAAGAG
TTCAACTTGTATTTCTATCACTTTTCCAGGGCAGAACAGTCCAAGGCAGAAGAAGCGCAA
CGGAAATTGAAAAGACAAAATAGAGAAGATACAGCCCTCCCACCTCCGGTGTTACCTCCA
TTCTGCCCTCTGTTTGCAAGCCTGGTTAACATTTTGCAGTCTGATGTCATGTTGTGCATT
ATGGGAACAATTCTGCAATGGGCCGTCGAACATAATGGATATGCCTGGTCAGAGTCCATG
CTGCAAAGGGTGTTACATTTAATTGGCATGGCACTACAAGAAGAAAAACAACATTTAGAG
AATGTCACGGAAGAGCATGTAGTAACATTTACCTTCACTCAGAAGATCAGCAAACCTGGT
GAAGCCCCAAAAAATTCTCCTAGCATACTAGCTATGCTCGAAACACTACAAAATGCTCCC
TACCTAGAAGTCCACAAAGACATGATTAGGTGGATACTGAAGACTTTTAATGCTGTTAAA
AAGATGAGGGAGAGTTCACCTACCAGCCCCGTGGCAGAGACAGAAGGCACCATAATGGAA
GAGAGTTCACGGGACAAAGACAAAGCTGAGAGGAAGAGAAAAGCTGAGATTGCCAGACTG
CGCAGAGAAAAGATCATGGCTCAGATGTCTGAAATGCAGCGGCATTTTATTGATGAAAAC
AAAGAACTCTTTCAGCAGACATTAGAACTCGATGCCTCAACATCTGCTGTTCTTGATCAT
AGCCCTGTGGCTTCAGATATGACACTTACAGCACTGGGCCCCGCACAAACTCAGGTTCCT
GAACAAAGACAATTCGTTACATGTATATTGTGTCAAGAGGAGCAAGAAGTTAAAGTGGAA
AGCAGGGCAATGGTCTTGGCAGCATTTGTTCAGAGATCAACTGTACTGTCAAAAAACAGA
AGTAAATTTATTCAAGATCCAGAAAAGTATGATCCATTATTCATGCACCCTGATCTGTCT
TGTGGAACACACACTAGTAGCTGTGGGCACATTATGCATGCCCATTGTTGGCAAAGGTAT
TTTGATTCCGTTCAAGCTAAAGAACAGCGAAGGCAACAGAGATTACGCTTACATACGAGC
TATGATGTAGAAAACGGAGAATTCCTTTGCCCCCTTTGTGAATGCTTGAGTAATACTGTT
ATTCCTCTGCTGCTTCCACCAAGAAATATTTTTAACAACAGGTTAAATTTTTCAGACCAA
CCAAACCTCACTCAGTGGATTAGAACAATATCTCAGCAAATAAAAGCATTACAGTTTCTT
AGGAAAGAAGAAAGTACTCCTAATAATGCCTCTACCAAGAATTCAGAAAATGTGGATGAA
TTACAGCTCCCTGAAGGGTTCAGACCTGATTTTCGTCCTAAGATCCCTTATTCTGAGAGC
ATAAAAGAAATGCTAACGACATTTGGAACAGCTACCTACAAGGTGGGACTGAAGGTTCAT
CCTAATGAAGAGGATCCTCGTGTTCCCATAATGTGTTGGGGTAGCTGCGCGTACACAATC
CAATCCATCGAAAGAATTTTGAGCGATGAAGATAAACCATTGTTTGGTCCTTTACCTTGC
AGGCTGGATGACTGTCTTAGGTCATTGACGAGATTCGCCGCTGCACACTGGACAGTGGCA
TCAGTTTCAGTGGTGCAAGGACATTTTTGTAAACTTTTTGCATCACTGGTGCCTAATGAC
AGCCATGAGGAACTCCCATGCATATTAGATATTGACATGTTCCATTTATTGGTGGGCTTG
GTCCTCGCATTTCCTGCGTTACAGTGTCAGGATTTTTCAGGGATCAGCCTTGGCACTGGA
GACTTGCACATTTTCCATCTGGTTACTATGGCACACATCATACAGATCTTACTTACTTCA
TGTACAGAAGAGAATGGCATGGATCAAGAAAATCCCCCCTGTGAAGAAGAATCAGCAGTG
CTGGCTTTATATAAAACACTTCACCAGTATACGGGAAGTGCCTTGAAAGAAATACCCTCT
GGCTGGCATCTGTGGAGGAGTGTCAGAGCTGGAATCATGCCTTTCCTGAAGTGTTCTGCT
TTATTTTTTCATTACTTAAATGGAGTTCCTTCTCCACCCGACATTCAAGTTCCTGGAACA
AGCCATTTTGAACATTTATGTAGCTATCTCTCTCTACCAAACAACCTTATTTGCCTTTTT
CAAGAAAATAGTGAGATAATGAATTCACTGATTGAAAGTTGGTGCCGTAACAGTGAAGTT
AAAAGGTATCTAGAAGGTGAAAGAGATGCTATAAGATATCCAAGAGAATCTAACAAATTA
ATAAACCTTCCAGAGGATTACAGCAGCCTCATTAATCAAGCATCCAATTTCTCGTGCCCG
AAATCAGGTGGCGATAAGAGCAGAGCCCCAACTCTGTGCCTCGTCTGCGGATCCCTGCTG
TGCTCACAGAGCTACTGCTGCCAGACTGAACTGGAAGGGGAGGATGTAGGAGCCTGCACA
GCTCACACCTACTCCTGTGGCTCTGGAGTGGGCATCTTCCTGAGAGTACGGGAATGTCAG
GTGCTATTTTTAGCTGGCAAAACCAAAGGCTGTTTTTATTCCCCTCCTTACCTTGATGAC
TATGGCGAGACAGACCAGGGACTCAGAAGGGGAAATCCTTTACATCTGTGCAAAGAGCGC
TTCAAGAAGATTCAGAAGCTCTGGCACCAACACAGTGTCACAGAGGAAATTGGACATGCA
CAGGAAGCCAATCAGACACTCGTTGGCATTGACTGGCAACATTTA
Protein sequence predicted based on nucleotide sequence
MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQK EDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPT CVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPL VHLSEDVIARTYNIFAITFRYAVEILTWEKESELPADLEMVEKSDTYYCMLFNDEVHTYE QVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKV QVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLSDS KLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQ FFTAPTLARMLITEENLMSIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILD LKYVLISKPTEWSDELRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTL QMKLTHVISMMQDWCASDEKVLIEAYKKCLAVLMQCHGGYTDGEQPITLSICGHSVETIR YCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCA QVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDVVMLQTGVSMMDPNHFLMIMLSRFEL YQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVGQVNATDEIKR EIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKE FNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCI MGTILQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLENVTEEHVVTFTFTQKISKPG EAPKNSPSILAMLETLQNAPYLEVHKDMIRWILKTFNAVKKMRESSPTSPVAETEGTIME ESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDH SPVASDMTLTALGPAQTQVPEQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNR SKFIQDPEKYDPLFMHPDLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTS YDVENGEFLCPLCECLSNTVIPLLLPPRNIFNNRLNFSDQPNLTQWIRTISQQIKALQFL RKEESTPNNASTKNSENVDELQLPEGFRPDFRPKIPYSESIKEMLTTFGTATYKVGLKVH PNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVA SVSVVQGHFCKLFASLVPNDSHEELPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTG DLHIFHLVTMAHIIQILLTSCTEENGMDQENPPCEEESAVLALYKTLHQYTGSALKEIPS GWHLWRSVRAGIMPFLKCSALFFHYLNGVPSPPDIQVPGTSHFEHLCSYLSLPNNLICLF QENSEIMNSLIESWCRNSEVKRYLEGERDAIRYPRESNKLINLPEDYSSLINQASNFSCP KSGGDKSRAPTLCLVCGSLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQ VLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSVTEEIGHA QEANQTLVGIDWQHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
