Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00841309
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4731nts
Open reading frame: 1 to 4731
ATGCATAACACAGCAGATGAGGTGGTTTTGTATCACAAATTAGCAGGTCCGTGGGGAAGG
TGTACAGGAGACTGTGGTCCCGGAGGAGTGCAGAGTCGGGCAGTGTGGTGTTTTCATGTT
GACGGGTGGACAAGTCACCTGTCTAACTGTGGTGAGAGCAACAGGCCTCCAAAGGAAAGA
AGTTGTTTCCGAGTTTGTGACTGGCACAGTGACCTCTTTCAGTGGGAGGTTTCTGACTGG
CACCACTGTGTGCTTGTGCCTTACGCTCGCGGTGAAGTCAAGCCTCGGACCGCAGAGTGT
GTGACGGCTCAGCATGGACTGCAGCACCGGATGGTGCGCTGCATTCAGAAGCTGAACCGA
ACTGTGGTTGCAAATGAAATATGCGAACACTTTGCCCTTCAGCCTCCTACAGAACAGGCT
TGCCTCATTCCTTGTCCCCGGGATTGTGTAGTATCTGAGTTCTTACCATGGTCCAACTGT
AGTAAGGGATGTGGGAAGAAATTGCAGCATAGAACTCGCGCGGTCATAGCTCCCCCTCTC
TTTGGTGGTTTGCAATGTCCAAATCTGACTGAGTCAAGAGCCTGTGATGCTCCCATTTCC
TGTCCTCTTGGGGAAGAGGAATATACATTTAGCCTTAAGGTTGGACCATGGAGTAAATGC
AGACTGCCTCATCTTAAAGAAATTAATCCAAGCGGAAGAACTGTTCTGGATTTTAACTCC
GATTCCAATGAGCGAGTCACCTTTAAACATCAAAGTTACAAAGCACATCATCATTCGAAG
TCTTGGGCAATAGAGATAGGTTATCAAACCCGGCAGGTTTCGTGTACAAGAAGTGATGGA
CAAAATGCTATGTTAAGCCTTTGCCTTCAAGATTCCTTCCCATTGACTGTTCAGTCCTGC
ATCATGCCCAAAGACTGTGAAACCTCCCAGTGGTCCTCCTGGAGCCCCTGCTCCAAGACA
TGCCGTTCAGGGAGTCTGTTGCCAGGATTTAGGAGCAGGAGCCGGAACGTGAAGCACATG
GCTATTGGAGGTGGAAAGGAGTGTCCTGAACTTCTTGAGAAAGAGGCCTGCATTGTTGAA
GGAGAACTTCTGCAGCAATGTCCCAGATATTCCTGGAGAACTTCTGAATGGAAAGAATGC
CAAGTCTCTCTGCTCCTGGAGCAGCAGGATCCCCACTGGCATGTGACGGGACCCGTGTGT
GGCGGTGGGATCCAGACCCGGGAGGTGTACTGTGCCCAGAGCGTACCAGCAGCTGCCGCA
CTGAGGGCCAAGGAAGTCTCTAGACCTGTGGAAAAGGCATTATGTGTGGGACCCGCCCCG
TTGCCCTCTCAGCTCTGCAATATCCCTTGCTCTACGGACTGCATAGTATCTTCCTGGTCA
GCCTGGGGCCTGTGCATCCATGAAAACTGTCATGATCCTCAGGGCAAAAAAGGATTTAGA
ACGAGGCAGCGCCATGTCCTCATGGAATCTACAGGGCCTGCAGGGCATTGCCCTCATTTG
GTGGAGTCTGTTCCTTGTGAGGATCCAATGTGCTACCGATGGCTGGCATCAGAAGGGATC
TGTTTCCCTGATCATGGAAAATGTGGCCTGGGACATCGTATTCTGAAGGCCGTCTGCCAG
AATGACCGCGGAGAAGATGTATCAGGGAGTCTTTGCCCAGTTCCCCCTCCCCCTGAGAGG
AAGTCTTGTGAAATTCCCTGCAGAATGGACTGTGTGCTGAGCGAGTGGACGGAGTGGTCA
TCCTGTTCCCAGTCCTGTTCAAATAAAAACTCAGATGGGAAACAGACCAGGTCAAGAACT
ATCCTGGCACTGGCTGGGGAAGGTGGAAAGCCATGTCCCCCTAGTCAGGCTCTCCAAGAG
CATCGTTTGTGTAATGACCATTCCTGTATGCAGCTTCACTGGGAGACATCGCCTTGGGGC
CCTTGTTCTGAGGACACATTGGTAACTGCCCTTAATGCAACCATTGGCTGGAATGGAGAA
GCCACGTGTGGTGTAGGCATTCAGACTCGGAGAGTCTTCTGTGTCAAGAGTCACGTGGGA
CAAGTAATGACCAAAAGATGTCCAGATTCTACTCGACCTGAAACTGTGCGCCCCTGTTTT
CTCCCATGCAAAAAAGACTGTATTGTGACTGCTTTCAGTGAGTGGACACCCTGCCCAAGG
ATGTGCCAAGCAGGAAATGCCACCGTAAAACAGTCTCGATACAGAATCATCATCCAAGAA
GCAGCCAATGGAGGCCAGGAATGCCCAGATACCTTATATGAGGAGAGAGAGTGTGAAGAT
GTTTCCTTGTGTCCTGTATATCGGTGGAAGCCACAGAAATGGAGCCCTTGCATCTTAGTG
CCAGAGTCTGTCTGGCAGGGAATAACGGGCAGCAGTGAAGCCTGTGGAAAGGGGTTACAA
ACAAGGGCTGTCTCATGCATCTCTGATGACAACCGGTCAGCAGAAATGATGGAATGCCTC
AAGCAGACAAACGGCATGCCTCTCCTTGTGCAAGAATGCACAGTCCCATGTCGAGAAGAC
TGCACCTTCACTGCTTGGTCCAAGTTTACGCCCTGCTCTACGAACTGTGAAGCCACAAAA
AGCAGGCGGCGACAGCTTACAGGGAAAAGCAGAAAGAAGGAGAAATGCCAGGATTCTGAC
CTTTACCCTCTAGTGGAGACAGAACTATGTCCTTGTGATGAATTTATATCCCAACCTTAT
GGAAACTGGTCAGATTGCATTCTTCCAGAAGGCAGAAGGGAGCCTCACAGAGGACTGCGG
GTACAAGCAGACAGCAAAGAATGTGGAGAAGGCCTGCGCTTTCGAGCAGTAGCTTGTTCT
GATAAAAATGGAAGACCTGTTGACCCCTCCTTCTGCAGCAGCTCCGGTTACATTCAAGAA
AAATGTGTCATTCCCTGCCCATTTGATTGCAAGTTAAGCGATTGGTCTAGTTGGGGGTCT
TGCAGTTCATCTTGTGGAATTGGAGTGAGGATTCGATCCAAATGGCTAAAAGAAAAACCT
TACAATGGAGGACGACCATGTCCCAAACTGGATCTCAAGAATCAGGCTCAGGTACATGAG
GCAGTCCCATGTTACAGTGAGTGCAATCAGTATTCCTGGGTTGTGGAACACTGGTCTTCA
TGCAAAATCAACAATGAGCTGAGGTCCCTGCGGTGTGGAGGAGGAACACAATCTAGGAAA
ATCAGATGTGTGAATACTGCGGATGGTGAAGGTGGAGCAGTGGATAGCAACCTGTGCAAC
CAGGATGAAATTCCCCCAGAAACCCAGTCCTGTTCTCTTATGTGTCCCAATGAGTGTGTC
ATGTCTGAGTGGGGACTTTGGAGCAAATGCCCACAGTCATGCGATCCCCACACAATGCAG
AGAAGAACTCGCCACCTCCTAAGACCATCACTGAACTCAAGGACTTGTGCTGAAGACTCA
CAGGTGCAGCCTTGCCTCCTGAATGAAAATTGCTTCCAGTTCCAGTACAATCTAACAGAG
TGGAGCACATGCCAGCTGAGTGAAAACGCACCCTGTGGTCAAGGCGTCAGGACCCGCCTG
CTAAGCTGTGTGTGCAGTGATGGCAAGCCAGTCAGCATGGACCAATGTGAGCAGCATAAT
TTGGAGAAGCCCCAGAGAATGAGCATTCCCTGCTTGGTGGAATGCGTGGTCAACTGTCAG
CTCTCAGGGTGGACGGCTTGGACAGAGTGTTCACAGACCTGTGGCCATGGAGGTCGAATG
AGCCGGACTCGATTTATCATTATGCCAACCCAAGGAGAAGGACGGCCATGCCCCACAGAG
CTTACCCAGGAGAAAACCTGCCCAGTGACCCCCTGCTACAGTTGGGTCCTGGGCAACTGG
TCTGCATGTAAATTGGAGGGTGGAGACTGTGGGGAAGGAGTTCAGATCCGCAGCCTTTCC
TGCATGGTCCACAGTGGTTCAATATCTCATGCAGCTGGACGTGTCGAGGATGCACTGTGT
GGAGAAATGCCCTTTCAGGACAGTATCCTGAAGCAGCTGTGTTCCGTGCCTTGCCCAGGA
GACTGCCATTTAACAGAATGGTCAGAGTGGAGCACATGTGAATTAACCTGCATTGATGGA
AGAAGCTTTGAGACTGTGGGCCGCCAGTCTAGATCAAGGACTTTTATAATTCAGTCTTTT
GAGAACCAAGACAGCTGCCCCCAACAGGTTCTAGAAACACGCCCTTGTACAGGAGGCAAA
TGTTATCACTACACATGGAAAGCAAGTCTTTGGAACAATAACGAACGAACTGTATGGTGC
CAGCGTTCAGATGGCGTTAATGTCACAGGAGGCTGCTCCCCTCAGGCCCGTCCTGCTGCC
ATTCGGCAGTGCATTCCAGCCTGCAGAAAACCTTTCTCCTACTGTACACAGGGTGGAGTC
TGTGGTTGTGAGAAGGGCTATACAGAGATAATGAAATCCAATGGTTTCCTGGATTACTGC
ATGAAAGTACCAGGCTCCGAGGATAAAAAAGCCGATGTGAAAAACCTTTCTGGGAAAAAC
AGACCTGTGAATTCAAAAATACATGATATTTTTAAAGGATGGTCCCTTCAACCACTTGAT
CCAGATGGCCGAGTAAAAATTTGGGTTTATGGCGTGTCTGGTGGCGCTTTTCTCATCATG
ATTTTCCTAATATTTACTTCCTACCTTGTTTGCAAGAAGCCAAAACCACATCAAAGCACA
CCTCCCCAACAGAAGCCTCTGACCTTAGCCTACGATGGAGACTTAGACATG
Protein sequence predicted based on nucleotide sequence
MHNTADEVVLYHKLAGPWGRCTGDCGPGGVQSRAVWCFHVDGWTSHLSNCGESNRPPKER SCFRVCDWHSDLFQWEVSDWHHCVLVPYARGEVKPRTAECVTAQHGLQHRMVRCIQKLNR TVVANEICEHFALQPPTEQACLIPCPRDCVVSEFLPWSNCSKGCGKKLQHRTRAVIAPPL FGGLQCPNLTESRACDAPISCPLGEEEYTFSLKVGPWSKCRLPHLKEINPSGRTVLDFNS DSNERVTFKHQSYKAHHHSKSWAIEIGYQTRQVSCTRSDGQNAMLSLCLQDSFPLTVQSC IMPKDCETSQWSSWSPCSKTCRSGSLLPGFRSRSRNVKHMAIGGGKECPELLEKEACIVE GELLQQCPRYSWRTSEWKECQVSLLLEQQDPHWHVTGPVCGGGIQTREVYCAQSVPAAAA LRAKEVSRPVEKALCVGPAPLPSQLCNIPCSTDCIVSSWSAWGLCIHENCHDPQGKKGFR TRQRHVLMESTGPAGHCPHLVESVPCEDPMCYRWLASEGICFPDHGKCGLGHRILKAVCQ NDRGEDVSGSLCPVPPPPERKSCEIPCRMDCVLSEWTEWSSCSQSCSNKNSDGKQTRSRT ILALAGEGGKPCPPSQALQEHRLCNDHSCMQLHWETSPWGPCSEDTLVTALNATIGWNGE ATCGVGIQTRRVFCVKSHVGQVMTKRCPDSTRPETVRPCFLPCKKDCIVTAFSEWTPCPR MCQAGNATVKQSRYRIIIQEAANGGQECPDTLYEERECEDVSLCPVYRWKPQKWSPCILV PESVWQGITGSSEACGKGLQTRAVSCISDDNRSAEMMECLKQTNGMPLLVQECTVPCRED CTFTAWSKFTPCSTNCEATKSRRRQLTGKSRKKEKCQDSDLYPLVETELCPCDEFISQPY GNWSDCILPEGRREPHRGLRVQADSKECGEGLRFRAVACSDKNGRPVDPSFCSSSGYIQE KCVIPCPFDCKLSDWSSWGSCSSSCGIGVRIRSKWLKEKPYNGGRPCPKLDLKNQAQVHE AVPCYSECNQYSWVVEHWSSCKINNELRSLRCGGGTQSRKIRCVNTADGEGGAVDSNLCN QDEIPPETQSCSLMCPNECVMSEWGLWSKCPQSCDPHTMQRRTRHLLRPSLNSRTCAEDS QVQPCLLNENCFQFQYNLTEWSTCQLSENAPCGQGVRTRLLSCVCSDGKPVSMDQCEQHN LEKPQRMSIPCLVECVVNCQLSGWTAWTECSQTCGHGGRMSRTRFIIMPTQGEGRPCPTE LTQEKTCPVTPCYSWVLGNWSACKLEGGDCGEGVQIRSLSCMVHSGSISHAAGRVEDALC GEMPFQDSILKQLCSVPCPGDCHLTEWSEWSTCELTCIDGRSFETVGRQSRSRTFIIQSF ENQDSCPQQVLETRPCTGGKCYHYTWKASLWNNNERTVWCQRSDGVNVTGGCSPQARPAA IRQCIPACRKPFSYCTQGGVCGCEKGYTEIMKSNGFLDYCMKVPGSEDKKADVKNLSGKN RPVNSKIHDIFKGWSLQPLDPDGRVKIWVYGVSGGAFLIMIFLIFTSYLVCKKPKPHQST PPQQKPLTLAYDGDLDM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
