Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments:
None
Publications:
PMID:16377204 Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics
EvNO00084282
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA:
PSI,Promega
Recommended Growth Condition:
Distributed in bacterial strain:
BR610
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.