Detailed Vector Information: pEU-His-FV

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pEU-His-FV
Source:
CESG
Description:
Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments:
None
Publications:
PMID: 15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
PMID: 23662776
Title: Structural and functional characterization of CalS11, the TDPrhamnose 3-O-methyltransferase involved in calicheamicin biosynthesis
PMID: 18765284
Title: Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.
PMID: 19892197
Title: Cell-free translation of integral membrane proteins into unilamelar liposomes.
PMID: 20637905
Title: Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins.
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
PMID: 23662776
Title: Structural and functional characterization of CalS11, the TDPrhamnose 3-O-methyltransferase involved in calicheamicin biosynthesis
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics
EvNO00084283
Price: 
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AVAILABLE
No restriction
Special MTA: PSI,Promega

Recommended Growth Condition:

Distributed in bacterial strain:
BR610
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.