Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
1191.1.25_GO.18701
Keyword:
None
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00084307
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1830nts
Open reading frame: 28 to 1857

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGAGTGTTGAA
GGTGATGATGATTTCGGAAGTTCTTCGTCAAGGTCTTATCAAGATCAACTATACACAGAG
CTATGGAAAGTTTGTGCAGGTCCATTAGTGGAAGTTCCTCGTGCTCAAGAGAGAGTTTTC
TACTTCCCTCAGGGTCACATGGAACAACTTGTGGCGTCAACTAATCAAGGAATCAATTCA
GAAGAAATACCTGTTTTTGATCTTCCTCCAAAGATACTTTGTCGAGTTCTTGATGTCACT
TTAAAGGCGGAGCATGAAACAGATGAGGTTTACGCTCAGATCACATTACAACCAGAGGAA
GATCAAAGTGAACCAACAAGTCTTGATCCACCTATTGTTGGACCAACTAAGCAAGAGTTT
CATTCGTTTGTTAAGATTTTAACGGCTTCAGATACAAGCACTCATGGTGGATTCTCTGTT
CTTCGTAAACACGCCACTGAATGCTTGCCTTCTTTGGATATGGCACAAGCTACTCCTACT
CAAGAACTTGTGACTAGAGATCTTCATGGCTTTGAATGGAGGTTTAAGCATATATTCAGA
GGACAACCACGGAGGCATTTGCTTACTACGGGTTGGAGTACATTTGTATCCTCGAAAAGA
CTTGTAGCTGGAGATGCTTTTGTGTTCTTGAGGGGTGAGAATGGGGATTTACGGGTTGGA
GTGAGACGATTAGCTCGGCATCAAAGCACAATGCCTACTTCGGTTATTTCAAGTCAGAGC
ATGCATTTGGGAGTTCTTGCTACAGCTTCTCATGCTGTGCGTACAACAACAATCTTTGTT
GTCTTTTACAAGCCTAGGATAAGCCAATTCATAGTTGGGGTGAACAAGTATATGGAAGCT
ATAAAGCATGGATTTTCTCTCGGTACCCGATTCAGAATGAGGTTTGAAGGAGAAGAGTCT
CCTGAGAGAATATTTACTGGTACGATTGTGGGAAGTGGAGATCTATCTTCACAATGGCCA
GCTTCTAAATGGAGGTCATTGCAGGTACAATGGGATGAGCCAACAACAGTTCAGAGACCA
GATAAAGTCTCACCATGGGAGATAGAGCCTTTCTTGGCAACTTCCCCAATTTCAACTCCT
GCTCAACAACCACAATCGAAATGCAAGCGGTCAAGACCCATCGAGCCATCAGTTAAAACA
CCAGCCCCACCTAGTTTCTTGTACAGCCTCCCTCAGAGCCAAGATTCCATTAATGCATCC
CTTAAACTGTTTCAAGATCCATCACTTGAGAGAATTTCAGGTGGATACTCCTCAAACAAC
AGCTTCAAACCCGAGACTCCTCCTCCTCCAACGAATTGTAGCTATAGGTTGTTTGGATTT
GATCTCACAAGCAATTCTCCTGCTCCAATCCCTCAAGACAAGCAACCGATGGATACTTGT
GGAGCTGCCAAGTGTCAAGAACCCATCACTCCAACCTCAATGAGTGAGCAGAAGAAGCAA
CAAACATCAAGAAGTCGAACTAAAGTGCAAATGCAAGGCATTGCGGTTGGTCGTGCGGTT
GATTTAACACTGTTGAAATCTTACGATGAACTGATTGATGAGCTTGAGGAGATGTTTGAG
ATTCAAGGACAGCTTCTTGCCCGAGACAAATGGATCGTTGTCTTCACTGATGATGAAGGA
GATATGATGCTTGCTGGTGATGATCCGTGGAATGAGTTTTGCAAGATGGCAAAGAAGATA
TTTATATATTCGAGCGATGAGGTTAAGAAAATGACAACGAAACTGAAGATTTCTTCGTCG
TTAGAGAATGAGGAATATGGTAATGAATCATTCGAAAATCGTAGTAGGGGGTGATAGGAC
CCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSASVEGDDDFGSSSSRSYQDQLYTELWKVCAGPLVEVPRAQERVFYFPQGHMEQ
LVASTNQGINSEEIPVFDLPPKILCRVLDVTLKAEHETDEVYAQITLQPEEDQSEPTSLD
PPIVGPTKQEFHSFVKILTASDTSTHGGFSVLRKHATECLPSLDMAQATPTQELVTRDLH
GFEWRFKHIFRGQPRRHLLTTGWSTFVSSKRLVAGDAFVFLRGENGDLRVGVRRLARHQS
TMPTSVISSQSMHLGVLATASHAVRTTTIFVVFYKPRISQFIVGVNKYMEAIKHGFSLGT
RFRMRFEGEESPERIFTGTIVGSGDLSSQWPASKWRSLQVQWDEPTTVQRPDKVSPWEIE
PFLATSPISTPAQQPQSKCKRSRPIEPSVKTPAPPSFLYSLPQSQDSINASLKLFQDPSL
ERISGGYSSNNSFKPETPPPPTNCSYRLFGFDLTSNSPAPIPQDKQPMDTCGAAKCQEPI
TPTSMSEQKKQQTSRSRTKVQMQGIAVGRAVDLTLLKSYDELIDELEEMFEIQGQLLARD
KWIVVFTDDEGDMMLAGDDPWNEFCKMAKKIFIYSSDEVKKMTTKLKISSSLENEEYGNE
SFENRSRG**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1830
Mutations:
None
Discrepancy:
a496g,T166A;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable