Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00843462
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 1848nts
Open reading frame: 274 to 2121
CCCGCAGGCTGCAGGGAGACCTTCCCCAGCCTGCAGCCCCAGGCCCGCCCCGCGTCACAT
GAGCCCCAGGGCTCCCACCCCCTCCCCAGGGCAGAGGACACCCAGTTGGTGGCCGGGAGG
GCCTCGGCTTTCCAGGGACAGAGGCCCAACTCCAGGACGCCCCAGCTGGCCCAGCCCCTC
CTCTTTCCCTCAAGGCTGCAGGAGGTCGGGAAAGGCAGTCCTGGTAGAGGCCTGTCCTGG
GCTCCAGGTTGGCCCCTGAGGGTGGCCCTCCTCATGCCGGCTTCAAGACTGAGGGACAGG
GCAGCCAGTTCAGCCTCGGGATCCACCTGTGGCTCCATGTCCCAGACGCACCCTGTGCTG
GAGAGCGGCCTCCTGGCATCTGCCGGCTGCTCCGCACCCCGGGGTCCCAGGAAGGGCGGC
CCAGCCCCAGTGGACAGGAAAGCTAAGGCCTCAGCGATGCCGGACTCCCCAGCGGAGGTG
AAGACGCAGCCCCGGTCCACACCCCCCAGCATGCCGCCCCCACCGCCTGCCGCATCCCAG
GGGGCCACACGCCCCCCCTCCTTCACGCCACACACACTGATGAACGGCAGCAGCCACTCA
CCAACAGCCATCAATGGTGCACCGTGCACACCCAACGGCTTCAGCAATGGCCCGGCCACC
TCGTCCACAGCCTCCTTGTCCACACAGCACCTGCCCCCAGCCTGCGGGGCCCGGCAGCTC
AGCAAGCTCAAGCGCTTCCTCACCACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAG
ATTGGGGAGCGCGTGCGCACACTGGTGCTGGGCCTGGTGAACTCGACATTGACGATCGAG
GAGTTTCATTCCAAGCTTCAGGAGGCCACCAACTTCCCTCTGCGGCCGTTTGTCATTCCC
TTCCTGAAGGCAAACCTGCCCTTGCTGCAGCGGGAGCTCCTGCACTGTGCACGCCTGGCC
AAGCAGACGCCCGCCCAGTACTTGGCCCAGCATGAGCAGCTCCTGCTGGACGCCAGCGCC
TCCTCCCCCATCGACTCCTCAGAGCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGG
ACGCCCGACAGGACCAAAGAGAACGGGTCAGACCGCGACCCGCTGCACCCCCAGCGCTAC
AGCCCCAGCAACGGGCCACCGCAGCCCACACCGCCGCCGCACTACCGCCTGGAGGACATA
GCCATGGCCCACCACTTCCGAGATGCCTACCGCCACCCAGACCCCCGGGAGCTACGAGAG
CGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAAGAAGTGATCGACCACAAGCTC
ACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAACAACCTCCTGAACTGCATCATG
GACATGGTGGAGAAGACGCGGCGCTCGCTCACGGTGCTGCGCAGGTGCCAGGAGGCCGAC
CGCGAGGAGCTCAACCACTGGGCGCGGCGCTACAGCGACGCCGAGGACACAAAGAAGGGC
CCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCCGGTCCCGAAGGGCCTCAGCTA
GACGTGCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGCTACGTGCCTGAGGACATCTGG
AGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAGGCCATGTCGGAGCTGCAGAAA
GCCGTGTCGGACGCGGAGCGCAAAGCGCACGAGCTCATCACCACGGAGCGTGCCAAGATG
GAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCCTCCGAGGACGCCCTGACGGTCATCAAC
CAGCAGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGGCGGAAAGCCAGTGAGACGTGC
AGCGGCTGCAACGCGGCACGCTACTGCGGGTCCTTCTGCCAGCATCGGGACTGGGAGAAG
CATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACAGCCGTGGTGGCCGACCCGGTG
CCTGGACCGCCCGAAGCCGCCCACAGCCTGGGCCCCTCCCTGCCTGTGGGTGCTGCCAGC
CCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGCCCCGGCTCCCCCAGCCCACCTGGCCCA
CTGGACACCGTGCCCCGCTGACCCCACTGGCCCCTGGCCTGCCGGACACAGCACCGTGCC
AACCCCACCCAGCTCCAGGCCCACCGGATGCTGTGCCTGGCCTCCGATGCCTGGCCTGCC
AGACACTGCGCCCCGCCTGACCTGGGGGAGCCGACCAATTAGTCACTGCTGCTACTGCCC
CTCTCCGAAAGAAGACACAGAACCAACAAAACCGCATTCAGTGCACCTGCCTCAGCTACC
TAATGATTCCGCGCGGAGACCTCCTGACAACGTCTCTTCAAGCATCCTCAGAAGCCTCGA
CTGAGCTTTAGACAGCAGAGCAGATGCCGCAGGCGCGGCGGCTCTGCCCACCTCTCTTTT
CCTCTCTGTCTGTCTCTCCCCCTCTGTCTTCTCTATCCTCTCTCTCTCTATGACTATCAC
ACACTTTCTCTTCAATGAAAAAATCGAATTGGTGGCTTATATTTTCAGCAAAGAATTTTG
GGGGGTTTTGTGTGTTGGCAAAAGAGCTACTCAGAAATGGACAAAGAAAACGGGGGGGTT
CTCCCCCTCCTGATTAAAAAGGGAGAAAGAAAACTGCGATTTTATAGCTGGAGATCTGAA
CCCAGCTGTGCCCCTCCCCCAGGGGCGTGAGGCTGATCAGCGAAGACGGGAGGAAAGATT
TCGATTTCTGACTCAAGATGCATTTTTGGTTTCAGATTTTTTTTTCCTGTAATGTTAAAC
TCTTTGGCTTTAAGTAAAAATCCAAAAAGTTTTTTTAAAAAAGCAAAGGAAGCATACTTG
TGAACTACCTTGCTAGCTAGCCAGCCAAGGATACCGGACACACCTCTGCTCCAAAGGAAA
TCCAAAAAAGCAAACACAAGAAATCAAAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MPASRLRDRAASSASGSTCGSMSQTHPVLESGLLASAGCSAPRGPRKGGPAPVDRKAKAS AMPDSPAEVKTQPRSTPPSMPPPPPAASQGATRPPSFTPHTLMNGSSHSPTAINGAPCTP NGFSNGPATSSTASLSTQHLPPACGARQLSKLKRFLTTLQQFGSDISPEIGERVRTLVLG LVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQTPAQYLAQH EQLLLDASASSPIDSSELLLEVNENGKRRTPDRTKENGSDRDPLHPQRYSPSNGPPQPTP PPHYRLEDIAMAHHFRDAYRHPDPRELRERHRPLVVPGSRQEEVIDHKLTEREWAEEWKH LNNLLNCIMDMVEKTRRSLTVLRRCQEADREELNHWARRYSDAEDTKKGPAPAAARPRSS SAGPEGPQLDVPREFLPRTLTGYVPEDIWRKAEEAVNEVKRQAMSELQKAVSDAERKAHE LITTERAKMERALAEAKRQASEDALTVINQQEDSSESCWNCGRKASETCSGCNAARYCGS FCQHRDWEKHHHVCGQSLQGPTAVVADPVPGPPEAAHSLGPSLPVGAASPSEAGSAGPSR PGSPSPPGPLDTVPR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
