Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00843509
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 1386nts
Open reading frame: 188 to 1573
GGAGGACGGACGGACAGGGCCAGCCTGCTGTCCGGCTGCCGCCCGCCGTGGTGTGAGGGG
GTTTCTGCGCACCCACAGTCGCCACCGTCCCACCTGGGCTGCCGGAGCCTCCCCCTGGAC
CCCTGGTGCCCACTGCCACCCTCATCCGGTGTGAGAGCGCTGCTTCCGCTTCGCGGACGC
CGCGGGCATGGACTATTCGTACGACGAGGACCTGGACGAGCTGTGCCCCGTGTGCGGGGA
CAAGGTGTCCGGCTACCACTACGGACTGCTCACGTGTGAGAGCTGCAAGGGCTTCTTCAA
GCGCACGGTGCAGAACAACAAGCACTACACGTGCACCGAGAGCCAGAGCTGCAAGATCGA
CAAGACGCAGCGCAAGCGCTGTCCCTTCTGCCGCTTCCAGAAATGCCTGACGGTGGGGAT
GCGCCTGGAAGCCGTGCGCGCTGACCGTATGAGGGGTGGCCGGAACAAGTTTGGGCCGAT
GTACAAGCGGGACCGGGCCCTGAAACAGCAGAAGAAGGCACAGATTCGGGCCAATGGCTT
CAAGCTGGAGACAGGGCCCCCGATGGGGGTGCCCCCGCCGCCCCCTCCCGCACCGGACTA
CGTGCTGCCTCCCAGCCTGCATGGGCCTGAGCCCAAGGGCCTGGCCGCCGGTCCACCTGC
TGGGCCACTGGGCGACTTTGGGGCCCCAGCACTGCCCATGGCCGTGCCCGGTGCCCACGG
GCCACTGGCTGGCTACCTCTACCCTGCCTTTCCTGGCCGTGCCATCAAGTCTGAGTACCC
GGAGCCTTATGCCAGCCCCCCACAGCCTGGGCTGCCGTACGGCTACCCAGAGCCCTTCTC
TGGAGGGCCCAACGTGCCTGAGCTCATCCTGCAGCTGCTGCAGCTGGAGCCGGATGAGGA
CCAGGTGCGGGCCCGCATCTTGGGCTGCCTGCAGGAGCCCACCAAAAGCCGCCCCGACCA
GCCGGCGGCCTTCGGCCTCCTGTGCAGAATGGCCGACCAGACCTTCATCTCCATCGTGGA
CTGGGCACGCAGGTGCATGGTCTTCAAGGAGCTGGAGGTGGCCGACCAGATGACGCTGCT
GCAGAACTGCTGGAGCGAGCTGCTGGTGTTCGACCACATCTACCGCCAGGTCCAGCACGG
CAAGGAGGGCAGCATCCTGCTGGTCACCGGGCAGGAGGTGGAGCTGACCACAGTGGCCAC
CCAGGCGGGCTCGCTGCTGCACAGCCTGGTGTTGCGGGCGCAGGAGCTGGTGCTGCAGCT
GCTTGCGCTGCAGCTGGACCGGCAGGAGTTTGTCTGCCTCAAGTTCATCATCCTCTTCAG
CCTGGATTTGAAGTTCCTGAATAACCACATCCTGGTGAAAGACGCTCAGGAGAAGGCCAA
CGCCGCCCTGCTTGACTACACCCTGTGCCACTACCCGCACTGCGGGGACAAATTCCAGCA
GCTGCTGCTGTGCCTGGTGGAGGTGCGGGCCCTGAGCATGCAGGCCAAGGAGTACCTGTA
CCACAAGCACCTGGGCAACGAGATGCCCCGCAACAACCTGCTCATCGAAATGCTGCAAGC
CAAGCAGACTTGAGCCTGGGCCGGGGGCGGGGCCGGGACTGGGGGCGGGACTGGGGGCGG
GGCCTGGGCGGGGCCGCAGCCACACCGCTGGCTCTGCATGGTTCATTTTCTGATGCCCAC
CGAGGAGCCCCAGCCCCGTCCCAGAGGCCGCTGCCCCTGAGTTCTGACACTGTGTGTTTG
GGAAGTGGGTGAGGCTGGGCAGGGCCTGGCGGAGGTGGAGTGGCCACTGGCACTTGCCTG
CTGCTTGGAGTGCCCCAAGGAGGTGGCTGTTAACCACCCGCCCCGCCCCCTCCCTGCTCC
CAGCTCTCTCTCCTGGAGTCTGAAGCCTGCAGGTCCGGGGAGGAGGTTCGGGATTCCCTG
GTGGGCCTCGACGTCCCTTGGATCAGAGGTCATCCCTTCCTCCTCTCCTGGAAACAGACA
GGGAGAAGTTGAGCAGGTATCAACTAGGGGAGGAGAGAGGGTCTCCAGTGTTCCCCCCAT
AGAGACCAGGAGGGAGAGCCTCTGTTTTGTAAACTAAGGATAACCGAGTTTGCTAAATTG
AGAGGGGCTATTGGGCCCTAGAGGACACTAGGAGACTGGTTAGGACAAAAAGACCTTCTC
CCTAGCCCTTCTACCCCACCTGACCTCTGCAAGAGGGGGCATTGATACATCATCGGGAAA
AAACTTTGCTCCAGGCATCACTGATTCCCTCTCCCACCCAAGGAGAACGTTTGGTACAAT
CGACATCCTAGCCCCACCCAGAGGTGGCCCTCCCAGGCTGGTATTTATCTGCAAGGTTGT
AGTCAAGAGGTTTTTCTCCCCGCTTTTTGTTTTTAAGCTTCTAGACACTCCTTGAAATGT
GTGTGTGATGGAGGGAAGGGGACAGATTTGAGGACTGAAGCTGGGGCTTGGGGATTGCCA
CTAAGTACAGCTGATGGTTTCTCCCCGGACACTCGCCTACTAAGTACCCTTGGGGTGGTG
CTGGGTCATTACTTCTGAGCCCCAGCCCCAATCCAGAGAAGCGCTGTTGCCCGCCCTCCA
CCCACTAGGTGAACAGCAGGATGCCCTGTTGGGGGCTTCAGGTCTCTGTGGGTGGGAATG
CAAGTGAACTTGGGAGGGGGCACGGGCCTGTAGATCAGGGATAGCGCTGTTGATCCCCTC
TCTGTGGCTCCAACCCGTTGGGTCCCTTGCTGCAAACCCATGAAGCTGGCCCTCAGCTCC
CTGACCCCCTGTCCTAGGTCATGAAGGACACTCTGCAGGGTGAAGCACCAGGGAGAGGCC
TCGGCTGTCTCCTGTCCCCGGCGGGGTGCCTGCTGTCCGTCCCGCTTTCATGTTACTGTT
GCAGCTTGTGCTGAGCCTGCCCAGTTGGAGGAGACTGGGCACCCCTGCCTCCTGCCTCCC
GCCTCCCGCCACCCTGTCTCAGTACCTCCCCCCCCCGCCCCCTGAAACATGTGCCCCTGC
CAAGGCCGGAGACCCACAGCCCTGAAACGAGAAGTGCCCTTAAGGATCACCCCAGCCCCC
ACAGCCCTGGAATAAATTTCGCAATTAGTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKT QRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKL ETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPL AGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQV RARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQN CWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLA LQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLL LCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
