Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00843598
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2121nts
Open reading frame: 236 to 2356
AGAGACCCACAAGTTCTATTCGGGGGGACCGACAGCCCGCCCCGGGAGGAAGGGGCGGCC
AGGCCCGAAAGCCGCCTCCCCCTCCCAGACCCGAGAGCTCGTGCGGGGCAAAGTGAACCG
AGCCGCTGGGCGGTGCAAGGGGAAGCCCAAGCCCGTTCTCCCGGCCAAAGTGAACTTTAA
TCGGGGTGGTTGGATGCGGAGACGGGGCGGCAGGACCTGCTAGAAGTGGCCGAAGATGAA
TCCCCAGCAACAACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGA
CTTCAGTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAACTAGACCAACTACACTGGG
AAGCAGTCAGTTCAGTGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAAC
AAGTGGTCAACCAAGTCCTTCCTATGATTCATCTAGAGGTTTTACAGACAGCCCTCATTA
CAGTGATCACTTGAATGACAGTCGATTAGGAGCCCATGAAGGCTTGTCCCCAACACCTTT
CATGAACTCAAATCTGATGGGAAAAACATCAGAGAGAGGCTCATTTTCCCTGTACAGCAG
AGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGACAAGATCTGGGGCTTGGGAG
CCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTAC
AAGTTCCAGGAGGAGACCACTCCATGACTCTGCAGCGCTTGATCCCTTGCAAGCAAAAAA
AGTCAGAAAGGTGCCTCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGA
TGATTTCAACCGTGAATCTCCTAGTTATCCATCTCCTAAGCCACCAACCAGTATGTTCGC
TAGCACTTTCTTTATGCAAGATGGGACCCACAATTCTTCTGACCTTTGGAGTTCATCAAA
TGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACTTCCCACATGTCTCA
ATCCAGTAGTTATGGCAACCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGT
TTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTAC
CAGCAGTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCAT
TCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACAGGTGATGCACTTGGAAAGGC
TTTGGCATCTATTTATTCTCCTGACCATACCAGCAGTAGTTTTCCGTCAAATCCATCAAC
ACCAGTTGGATCACCTTCACCTCTCACAGGTACCAGTCAGTGGCCAAGACCTGGAGGGCA
AGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGAAAAATCGAGTTGAGCA
GCAACTTCACGAGCATTTGCAAGATGCAATGTCCTTCTTAAAGGATGTCTGTGAGCAGTC
TCGAATGGAGGATCGTTTAGACAGACTGGATGATGCAATCCATGTGCTGCGGAACCATGC
TGTGGGACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACC
ATCCCATAATGCACCAATTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGC
AAGCAGTCGATCAGCTTCAATGGTTGGAACTCATCGGGAAGACTCTGTCAGTCTCAATGG
CAATCATTCAGTCCTGTCTAGTACAGTCACTACTTCAAGCACAGACCTGAACCATAAAAC
ACAAGAAAATTATAGAGGTGGCTTGCAAAGTCAGTCTGGAACTGTTGTTACAACAGAAAT
CAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGACAT
GAAGTCAGATGATGAATCCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAG
CAGTACTAATGAAGATGAGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAG
GCGGATGGCTAACAATGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAA
AGAGCTTGGCCGAATGTGTCAGCTTCACTTGAAGAGTGAAAAACCCCAAACAAAACTCCT
TATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTCAGAGAGAGGAA
CCTTAACCCCAAAGCAGCCTGCCTTAAGAGAAGGGAAGAAGAAAAAGTTTCTGCCGTATC
GGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAACCC
TATGGGTCATATGTAAACATCAGCCAGTTCCAGAGTTATCAGTAGGCTAGATAGAAGGTG
ACCTCTCCTCATAAGGACTTGGACAACTCAGATTATCTGAAGACACAAACCTGACAGGAG
GGAGAAGAAAAAACAAAACACTTGAACCAAGAAACTCAAATGTAATCCTACGATCAAAGC
AACTGGTCAACACTTCCATCAGAAGTGAAGATAGGAAGCTCATCAGATAGAACATCAGCC
CATGAGATGTTTGCAACAAATCTTTTGTTGCAAGCAGTGTGTCGCTTCTGCACAATCAGA
GACTGTCTCGATCTCTCCACTCACCGTGGAAGTTGCCTTGTGCCTAAACTGAATTGACAA
ATGCATTGTAACTACAAATTTTATTTATTGTTATGAAACTGTAAGGTCTACATATAAAGG
GAAAAAGTTAATGTGGAAAGCTGATCTACACTCAGCTGATGCCAGCATACATTAAAGCGG
TTCACGTGCAGAGAACAAAGCAGTGACAACCATTGGCCCTTAGCATTCCCGGCATACCTA
TTAGTGTCTTAAAAAGGAAGGGAAAAGTCTTTTGTTGCCCTCTCCTATCCTCTTGCCATA
TGAATAGCGTTTTCCATGAAATAGGAAAATATTACTTGGTATAGCATTTCTCTTGCTCTC
ATTTTTTGATTTATTTTTATTTTCTCTTTGTGGGTGTTATATTTGATCTCTAAATCTGAA
CAGTTTATGGTCACAGTCCAGCCTCCTCCGTGCAGCCCTGTGTGCTTTGCACATTTACCT
TACAGTGGTAAGCAGAGACCATCTGTGACCATAGCCTAGCTAGCATTTTAAAAGGGGAAA
TTTTGTTCTCTAGGTTTTCCCCCAAATAAACATTGCTTTATTTCTAATAATAACCAAGAC
TTTTCAAGCTTCTAGATCTCATAGGAAAGCTTGTAATAGCAAAATTGTAAATTACAAGGG
AAGAATCTACTTTTTAGAAATCGCTTTGTTTTCCAAGCAGTAAGTACTACATACAGTACT
TGTAAAGTGTTAGCTGTAAGTAAGCACAAAATACATTTAAAATACAAAGACGATTTTTTC
AGGCTGTGATTATGGTGAACATAACAAAACCCAGTAGTCACCAAGGCAGGTAGTGTGATA
AATGAACACACCACTCTGAGGCTAATTACCTAATGGAATACAAGAGCAATGGTCACCCGT
ATTTCCTTATCCTAGCCTTTATTTCTCTGTCATTTGGATGGCTGGTCAATGGGGAAGAAT
TGAGTGGGTGATTTAATCAACTGCAAACCATCTGCCCCTGTCCCAAAATGATGAGCCAGA
TTAGCATTAAACCAGTACTTGTCAGTCCATCTTAATACTGTTCATTAAGGCACTCTCTGT
CTCTAATCCTTAGGAGTTGTTTTAAAAGACATAATCACTTTGAACTTCCATGAAACCTGT
CTTCCACCACAACAACCCTGGGAGAGAAAAACATGCTAAAGGAGGTATCTTGGCTTAATA
ATTCCTTATAGCCAATATCAACAGTGGCAATCAGCACACAGAGGAAAGGACCCAAATCAC
TATGTAGCTTAAAGATTTCTGTTAATTTGAAAGAACAAAAACAAGACAGAACTTCTGGTA
CTCTAATCAGGATGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTGGTT
ACTTTAATTTGCATATATTCTCCAGTTACATCGGACTCTATCTGTGGCCTTGTTCTTCAT
TTCAGTGTTAATCAGCTAAACAGAAGTTGTTGCTTATGATGTGTGAGTGAACATATGCCA
CTGCCTGGCCTTTTTTTCTTCAGAGCTTGTTGTCTTTTTCGCTATATTAGACTTTGCAGT
ATGCCCAGAAGCTTTCCTTCATAAAATAGAAAGAAAAAAACATTTGGCTTATTTTTCACT
GTAGCTAGTCTTTTATACAATAATCTTGTAAGAAAATTTCTTGAATTCTAAATATTACTC
TTTCTAGATTTTTGAAATCAAAAAGTTTTCAGTAAAAAGTTTCTTACTTTATTTTATTAT
ATTAGGTAGTAAAAAATGTAGGGTTATTTACCATAACCTGTTCATTAATATCAGAAATTT
ACAATAGCATTTTAAGACCATAGTAGGATTCTAGCATACCGTGTAGTACCTATGGAGTAT
TGTAAGAGCTAATTGTTGGAGATGAATTGCTTCTCATCTTGTTCTCCAGTTTCCATTGTT
GGTTTATTGCAGATTTGTATCCTGTGTCAAATTCAAGGTATTATTGATAAACCTTTTCAA
CCAGCAGCAAGAAGTTCAAATTTTTTTCTGTCACTGTAACAGAAAACACAATATGTATAT
AACATTTATGTAGCAATAAATGTGCCATCTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAA
AAAAA
Protein sequence predicted based on nucleotide sequence
MNPQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPTTLGSSQFSGSGIDERGGTTSW GTSGQPSPSYDSSRGFTDSPHYSDHLNDSRLGAHEGLSPTPFMNSNLMGKTSERGSFSLY SRDTGLPGCQSSLLRQDLGLGSPAQLSSSGKPGTAYYSFSATSSRRRPLHDSAALDPLQA KKVRKVPPGLPSSVYAPSPNSDDFNRESPSYPSPKPPTSMFASTFFMQDGTHNSSDLWSS SNGMSQPGFGGILGTSTSHMSQSSSYGNLHSHDRLSYPPHSVSPTDINTSLPPMSSFHRG STSSSPYVAASHTPPINGSDSILGTRGNAAGSSQTGDALGKALASIYSPDHTSSSFPSNP STPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCE QSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSL VASSRSASMVGTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTT EIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREK ERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRE RNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
