Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00843606
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2019nts
Open reading frame: 156 to 2174
GGGCAGGAGGCGGCGGCCGCGTCGACGTCGACCCAGACTGGAGCGACGTTTAAAGAAGGG
GCAGAATCGCTGGGGAGTGCGGCTTCTTCTTGTTGGGGGACTCCCAGCCTTCCGCGCGTC
CGGAGGAGGAGAAGCGGCGGCGCCGGGAAGCAGGCATGGAGAGTAGAAAACTGATTTCTG
CTACAGACATTCAGTACTCTGGCAGTCTGCTGAACTCCTTGAATGAGCAACGTGGCCATG
GACTCTTCTGTGATGTTACCGTTATTGTGGAAGACCGAAAATTCCGGGCTCACAAGAATA
TTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTCTTCTCTGTTGCTGGGCAAGTTGTTG
AACTGAGCTTTATAAGAGCAGAGATCTTTGCAGAAATTCTCAATTATATCTATAGTTCTA
AAATTGTTCGTGTTAGATCAGATTTGCTTGATGAGTTAATTAAATCAGGGCAGTTATTAG
GAGTGAAATTTATAGCAGAGCTTGGTGTCCCATTGTCACAGGTTAAAAGCATCTCAGGTA
CAGCGCAGGATGGTAATACTGAGCCTTTACCTCCTGATTCTGGTGACAAGAACCTTGTAA
TACAGAAATCAAAAGATGAAGCCCAAGATAATGGGGCTACTATAATGCCTATTATAACAG
AGTCTTTTTCATTATCTGCCGAAGATTATGAAATGAAAAAGATCATTGTTACCGATTCTG
ATGATGATGATGATGATGTCATTTTTTGCTCCGAGATTCTGCCCACAAAGGAGACTTTGC
CGAGTAATAACACAGTGGCACAGGTCCAATCTAACCCAGGCCCTGTTGCTATTTCAGATG
TTGCACCTAGTGCTAGCAATAACTCGCCCCCTTTAACAAATATCACACCTACTCAGAAAC
TTCCTACTCCTGTGAATCAGGCAACTTTGAGCCAAACACAAGGAAGTGAAAAATTGTTGG
TATCTTCAGCTCCAACACATCTGACTCCCAATATTATTTTGTTAAATCAGACACCACTTT
CTACACCACCAAATGTCAGTTCTTCACTTCCAAATCATATGCCCTCTTCAATCAATTTAC
TTGTGCAGAATCAGCAGACACCAAACAGTGCTATTTTAACAGGAAACAAGGCCAATGAAG
AGGAGGAGGAGGAAATAATAGATGATGATGATGACACTATTAGCTCCAGTCCTGACTCGG
CCGTCAGTAATACATCTTTGGTCCCACAGGCTGATACCTCCCAAAATACCAGTTTTGATG
GATCATTAATACAGAAGATGCAGATTCCTACACTTCTTCAAGAACCACTTTCCAATTCCT
TAAAAATTTCAGATATAATTACTAGAAATACTAATGATCCAGGCGTAGGATCAAAACATC
TAATGGAGGGTCAGAAGATCATTACTTTAGATACAGCTACTGAAATTGAAGGCTTATCGA
CTGGTTGCAAGGTTTATGCAAATATCGGTGAAGATACTTATGATATAGTGATCCCTGTCA
AAGATGACCCTGATGAAGGGGAGGCCAGACTTGAGAATGAAATACCAAAAACGTCTGGCA
GCGAGATGGCAAACAAACGTATGAAAGTAAAACATGATGATCACTATGAGTTAATAGTAG
ATGGAAGGGTCTATTATATCTGTATTGTATGCAAAAGGTCATATGTCTGTCTGACAAGCT
TGCGGAGACATTTTAACATTCATTCTTGGGAGAAGAAGTATCCGTGCCGTTACTGTGAGA
AGGTATTTCCTCTTGCAGAATATCGCACAAAGCATGAAATTCATCACACAGGGGAGCGAA
GGTATCAGTGTTTGGCCTGTGGCAAATCTTTCATCAACTATCAGTTTATGTCTTCACATA
TAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTTATCGTTTACATCCAT
GCAGGTCTTTACAAATCAGACAATATGCATATCTTTCCGATAGATCAAGCACTATTCCTG
CAATGAAGGATGATGGTATTGGGTATAAGGTTGACACTGGAAAAGAGCCTCCAGTAGGGA
CCACTACATCTACTCAGAACAAGCCAATGACCTGGGAAGATATTTTTATTCAGCAGGAAA
ATGATTCAATTTTTAAACAAAATGTAACAGATGGCAGTACTGAGTTTGAATTTATAATAC
CAGTGTCTTACTAAACTCCTTTGAAATACTAGAAAGTTTTGTTTTGGATGATGGGGCAGG
GGTTTCAGAAGATCTGTAAAACAAATTAAGGTGCGAACAAGTTAATTTGATCTGCCACAT
TATCTGAAGGAAGTGTAGTGGGATTTTTGTTGATAATTTTTAGAAGCAAATTTTCCTGAA
AGTTTTGAGTAGAGGTGAGACCCCCTCCCCAAGTATCTGTTTATATAGTTAGTTTTCAGC
TCATTTAAAAGAGGCAAAAATTAAAAGCTTGGAGAGATAGTTTCCTGAATAGAATTTGAA
GCAGTCTGAATGTTCTTTGAAAATAACTGGAGTTATTAGCATACCCTAGTACATCTTACA
GCTTTCCCCTTCCATGTTAGCACTTTACTGCTGAATTCTCAATTTTCTTAACATTGAGAC
AATAAATGTGTGTTTTGTCTTGTATATGGCATAAAGAGTAAATAAGTTTTAGAGTTGTTC
TGGAAAATGTCAGAATAAGTCAGTACTTGGGTTGTGTAATCTGCTAGTCCAAGCGAACAG
CAACCTCCTGCTACCCTCCCTCTATGAAAATAGCCATGCAGACAAGTCTCTCATCTGAAG
AACAAATTAGATTTAGCTAATTAGAATTAATCCTGGCTTTCATTGCCATAGTCTGTAAAA
GACTTTGGTGGCTAGACCACTTTATACCTTCGCAGTGTGGTCTCTGGGGGCAAAAAACTA
ATGAAAACAATCTCTGTAATGGCAGATAGGAGGAGATGAAAAGTTCTGTTGCATGGATTT
TTAATTCTCTGGCTACCACATAGTAGAGAATGGAATGAAGATTTCCTTTTGGCTTCTTAA
GGTTAAAAATATTCCCATGAACATGAAAATTTTCAAATTTTGAATCTGAAAGCCACCAAA
TGTATCTTTATGTATAAATCCTTGTAAATGATAGATTCCATGGGTGAGACTTTACATATT
TTGGGTGGGAGGCTACTGGCATATATTTTTAAATGTTCATATTGCGTAGAATCTCCACTA
GGAAGTCTTTATTTGAAATAGTTGAATCAGTGATCTAGTATTTTCCTTTCGGCAAGATTT
GTTAGGTTTTTACCCCTTCTAAAATAAGTTTTATTCCATCTGCAAATTGCTGCAATATTA
TAGTAATCAGAAACTACATAAGGAATGTTATATAGGCTTGTCAGTTCCCATTTTTCTTGA
CAACAATAAATACCACTTTTAAAAATGACACATATTTAAACACTTAGAAAATAAAGTTAA
CACTTACTGAAGTGCTAGTACTAAACTGTGCTAGTACTAAAAGAAAACAGGTTGGAACAT
ACATATAGCCTAGCATTTATAACAGAATTGTTGAACGTCTGTAAATGATTTTTTTTTTTT
TGCAAAGGAAAAAATTGATACTGGAAAAGATTGTTGTGCATAGTTATTAGTCATTTGTAA
CCTTGCTTAAGTATTTCTTAGTCCAACATAGATATTTTCTTTCTCCTGACCATGTATTTT
AAAATATAGTCTATTTCTTGACTTTGAACTTAAAGCTTTAATCATAATTTCTCATGTATA
CATCGTTCTTCTGATGGTAAGCTGGATTTGAAGGTAGTGGTTTCAGTGTTTCTTAAGTTG
GTAGCTGAGGGTATCAGGCATCAGTTCATGCAATAATACAAGAAAAAAAATCCTTTGCTT
GCCAAGAGGTAGAGTGATGTGCATTTATCTGTTTTCTGTTCTGTAAGTCTAGACCTTCAA
ACCATTTGTAAACTAACCCCTGGGAAATTTGAAATTACCTGATAACTTAAGACTCTGTGA
TCTCTGGAATCACCATATGTTTCTTTTTTGTGTAGATATTAATAACATTACTCTTTGACT
ATAGTGTGCACTCTGAAATGTACTCAGTGAAAATTTGTTTTGAGTTTCATTAATGCTATT
TCACCAGTTAGACATAATTACTTCTACCGATGTGAATGATACGGATGCCGGCAGAGCTTC
CAGATCTTTCAGACTCAACTGCTAGGTCAATTAGTTTGTCATAATAAAACTTGGCAGATT
CTACAAGTCTATTATGACAAACCAGGAACTAATTCTATAATGGAAAACTATCCATTCTGA
ATAATAGGTATGTAATTATTTGCTGCTGCTGCTGTGCTCTGTAAATTCTGAATATGACAT
TTAAACTCTGTGCCTACTAAAGGTATCTTCTGGAGTTTTTGGGAGGAGAGAAACTGGAAA
ATTAAATTGTATTTTTGCCAGAAGACTCTTACTTGCATGTGTCTCAGGGTCTTCAGTTTT
TCTATAAGTTTCCATATCCAAAGTTCAGAATTCATGTGAAATACTTCTTTGGGGCAAAAG
TCCTTCATTCCTGGTATTTATTGGATTGGAAATCTGTAGCAAGATGCTGTTTAAAATTAC
CATATTGTTTTTTTATCTTATGCTTAGCTCTCTGGCTATTGAACTTCCTTTTCTTGTTTG
AAGTTAGCTTCAAATTTGCTCCTATGCTAAATTACCTGTAAATATTCTGGATAGGAACTA
CTTGAAATAGTAATTTGTTAAAAGATATGACAAAATGAAAATGCTTAAACTACAGAAATT
TAAAAATGCCATAACAATCTTGCGAGACTAACTTTAAAATATACTTTAAATGATTATTAT
GATTTTGGTGGTAACGATCCCCCACACACAACCACTATGAAGAAATAATGCCGCATTTTT
CCCCCATTGTACCAAAAAGATAAAAAAATGGTAAACACTGATCAAGGTATTTTGTATTGT
CAAGGCATGCATATTCTAAAGAATTAAATGCTAACTTAACAGCACTGGCTTTCTGGCTGG
TCAACTATATGAAACCTTGTTCATTCCTCCGAGTACTGTAATGTTCACACTTGTACAATC
TTCCCTGTCATGACTTTAAGTTCTACTTTTCATTAACCATGGCCTGATATTAGTTCTTAG
AGCTTCTTGTGGCAAAAATAAAATGATTTAATTCTAAAAAAAAAAAAAAAAAAAACA
Protein sequence predicted based on nucleotide sequence
MESRKLISATDIQYSGSLLNSLNEQRGHGLFCDVTVIVEDRKFRAHKNILSASSTYFHQL FSVAGQVVELSFIRAEIFAEILNYIYSSKIVRVRSDLLDELIKSGQLLGVKFIAELGVPL SQVKSISGTAQDGNTEPLPPDSGDKNLVIQKSKDEAQDNGATIMPIITESFSLSAEDYEM KKIIVTDSDDDDDDVIFCSEILPTKETLPSNNTVAQVQSNPGPVAISDVAPSASNNSPPL TNITPTQKLPTPVNQATLSQTQGSEKLLVSSAPTHLTPNIILLNQTPLSTPPNVSSSLPN HMPSSINLLVQNQQTPNSAILTGNKANEEEEEEIIDDDDDTISSSPDSAVSNTSLVPQAD TSQNTSFDGSLIQKMQIPTLLQEPLSNSLKISDIITRNTNDPGVGSKHLMEGQKIITLDT ATEIEGLSTGCKVYANIGEDTYDIVIPVKDDPDEGEARLENEIPKTSGSEMANKRMKVKH DDHYELIVDGRVYYICIVCKRSYVCLTSLRRHFNIHSWEKKYPCRYCEKVFPLAEYRTKH EIHHTGERRYQCLACGKSFINYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYL SDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDG STEFEFIIPVSY*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
