Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00843946
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 1392nts
Open reading frame: 2242 to 3633
GTTCCGGCTCCCGAGCCCCGCCTGCGCGCGGCCTCCTCGGCGCAGCCATCCTCTTGGCTG
CCGCGGGCGGCAAAGCCCACGGCATCTGCCATTTGTCATTCAGCCCGTCGGTACCGCCCC
GAGCCTTGATTTAGACACGGCTGGGGCGTGCTCTGGCCTCACTCTCCGGGCGGGTGCTGG
ACGGACGGACGGACGGGGCAGCCGTGCTCACAGCTCAGCAGCGCGGGGCCTTGGCGCGCG
GGGCGCTTCCCCGGGTCGCCGTCATGGCCGCGGAGGTGGCACGCCCGAGCGGCCTCGCCT
GAGCTCCGGGGGTCGTCGCCCCGCAGGGATTGCTGTCACGTCTAATGTGGCTGCTGCCTC
GTGTCACATCTGAAACTCATCTGTACCTCACTTAGAAAGTGGTTCTGATTAGACAAGACT
TTTCGTTGCAGTCGACAGAAACCTAATGGGACCATTGAAGAATTCCAAACAGGCAAGTGA
CAGGAACATATTTGCATGTTAGAAGAATCAGCCTGGCAGCAGCATTGTGATTAGAATGAA
GGGGAACCGTCCAAAAACAGACTGGGGAACCAATATGGATCTGCCTCTAGCATGCGGAAA
TATCTCTCTACACTGACCTAACTGACTCACTAAGCTTGTGGTTTGTTTAAAGGAAGCCAC
ATGAAAATTGAGTTTGGGCCAATAGAGTGAACTCGTTCTCCATTCTCTAGCATCCCTCCC
CATGCAACCACCCTATCCCTGCCAAGTTTCTGGGCACCACAGATTGGAAAGTAGCCTCCT
GTAGGTATTTGCCCATGTTAAGTGGTGGGGTCTCTTTCTGTCTTCCCTTCTTGGTCTCAC
CTGTCTGGTGTGACAAGGGAAGCATGTGCCAACCAAGGCTAGACCCTTGTGAGACCAGAG
CAGCCCCACTTTCGGTAAAGCAAGCAACCTCTGCTTACTTGCCCAACACACCCTAGCTTC
CGTGTTCCTTGCCTTGTGAGTTATCTTCTTGGGTACAATTTAACACAGCGTTCCCACCTC
CTTATAACTAAAATAGCTAGAGGGGGCTTTGTGCCTCAAACCCCAAGGAGACCATCTTGG
CAATCTCTGCCTCTGCAACAGGGCACTTGCTCCCTGGGCAACTCCTTTCCCAGGTCATCT
TCTCCCTTGACATGCCCATCAGATTTAATAGTGTGCCTATTTGGCAGAAAAATGGGGTCC
CTTGGTTGCTGATTCTTAGGAATGTGAGGTTCTCCCAGCCAGAGCTCTCAGCACTAAAAG
TTCTAGTCTCTGCGTAATGGCCGGCATATCTTTGGCGTATCTAAAATCCTCTTCAGTTTC
CAGCAGGGTCTTTATAAGCTTCTTCTGAAGGTCTCAGACATTCAGCCACGGAACTCAAGT
GGAATTTGTTTGGATAACGTGACTGATTTCAAAGCCCGGACCCCTTAGACGTGCCCATTT
GGTGCTGCAAGTACTGACCATTACTACAACATCTTACAGTCAACAAATTTGACACATTAA
AGATTTATATCCTTTCTTTTGGGTTAGGATCTTCTCTCCCCTAAAGCATCTCAGTTTCCA
GCATGCAATCATTTCCATCTTATGGAAATCAGCCATCCCGCTCCGTGCCAGCATGCTACC
CTGGGAGGCACATCCAGGCTTGGGAAACGGGGGTGTCCTGGATCTCATGACTCCAGCAGC
ACCAGCTGCTCTCTTTCCTCTTCCAAGTAGACTTCCGTTCCCCCCCCACTTGGGTGTTTT
TGTTTGTTTTAGCAATTCAGAGCTCAAGATAAAGACCTTAAAGATAACTTTGTGTGTCTC
TCCCTTTCTAGGTATTTGCATAGGAATCAGAGGAGTTAATCTTGTTTGAATCTTCAGACA
AACTTCTGGGAGGACTCGGTCCCTGCCTCGCAGCAGATGTTCCCTGTCACTCAGTAGCCA
ATCCGGGGGACCCAGGACATGCCCCAGCTATAGTGATGCAGATTACCTTTCTGCTCCTGA
ATCGCACCTGTGCCTCAGACTTTCTCCCCTCAGCTTGAGACTGCATGTAAACTGGGATGT
GTGAAAGCAGGAAGCAAAGCTAGTGACAGCTGAGAGGTCCATGTCTGGGTAGAACCAGGC
CCACGATGCTGCCTCTCCCGTGGTCTGGAGTTCAGCTGCAGGGACTCTGCTGATGTGCCC
AGCACCATCGTTCTGTTTGTGCTTAAATGGCACAGCATTTGGTCAGCACATCTGAAAAGG
AAGGTGTGAGAAGCAAAGCCCATGGCCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTC
CTCACCCTGGAGAAGTTCACGATTCACAATTATTCCCACTCCAGGGAGCGGCCGTACAAG
TGTGTGCAGCCTGACTGTGGCAAAGCCTTTGTTTCCAGATATAAATTGATGAGGCATATG
GCTACCCATTCTCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAGACGTTCAACCGG
AAAGACCACCTGAAAAACCACCTCCAGACCCACGACCCCAACAAAATGGCCTTTGGGTGT
GAGGAGTGTGGGAAGAAGTACAACACCATGCTGGGCTATAAGAGGCACCTGGCCCTCCAT
GCGGCCAGCAGTGGGGACCTCACCTGTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAG
GTGCTACTGGACCACCTCAAAGCCCATGCGGAAGAGAAGCCCCCTAGCGGAACCAAGGAA
AAGAAGCACCAGTGCGACCACTGTGAAAGATGCTTCTACACCCGGAAGGATGTGCGACGC
CACCTGGTGGTCCACACAGGATGCAAGGACTTCCTGTGCCAGTTCTGTGCCCAGAGATTT
GGGCGCAAGGATCACCTCACCCGGCATACCAAGAAGACCCACTCACAGGAGCTGATGAAA
GAGAGCTTGCAGACCGGAGACCTTCTGAGCACCTTCCACACCATCTCGCCTTCATTCCAA
CTGAAGGCTGCTGCCTTGCCTCCTTTCCCTTTAGGAGCTTCTGCCCAGAACGGGCTTGCA
AGTAGCTTGCCAGCTGAGGTCCATAGCCTCACCCTCAGTCCCCCAGAACAAGCCGCCCAG
CCTATGCAGCCGCTGCCAGAGTCCCTGGCCTCCCTCCACCCCTCGGTATCCCCTGGCTCT
CCTCCGCCACCCCTTCCCAATCACAAGTACAACACCACTTCTACCTCATACTCCCCACTT
GCAAGCCTGCCCCTCAAAGCAGATACTAAAGGTTTTTGCAATATCAGTTTGTTTGAGGAC
TTGCCTCTGCAAGAGCCTCAGTCACCTCAAAAGCTCAACCCAGGTTTTGATCTGGCTAAG
GGAAATGCTGGTAAAGTAAACCTGCCCAAGGAGCTGCCTGCAGATGCTGTGAACCTAACA
ATACCTGCCTCTCTGGACCTGTCCCCCCTGTTGGGCTTCTGGCAGCTGCCCCCTCCTGCT
ACCCAAAATACCTTTGGGAATAGCACTCTTGCCCTGGGGCCTGGGGAATCTTTGCCCCAC
AGGTTAAGCTGTCTGGGGCAGCAGCAGCAAGAACCCCCACTTGCCATGGGCACTGTGAGC
CTGGGCCAGCTCCCCCTGCCCCCCATCCCTCATGTGTTCTCAGCTGGCACTGGCTCTGCC
ATCCTGCCTCATTTCCATCATGCATTCAGATAATTGATTTTTAAAGTGTATTTTTCGTAT
TCTGGAAGATGTTTTAAGAAGCATTTTAAATGTCAGTTACAATATGAGAAAGATTTGGAA
AACGAGACTGGGACTATGGCTTATTCAGTGATGACTGGCTTGAGATGATAAGAGAATTCT
CGAACTGCATGTATTGTGCCAATCTGTCCTGAGTGTTCATGCTTTGTACCAAATTTAATG
AACGCGTGTTCTGTAATCAAACTGCAAATATTGTCATAACCAACATCCAAAATGACGGCT
GCTATATATAAGTGTTTGTCATATGGAATTTAATCGTAAGCCATGATCATAATGTTAACT
AAATAACTTTATGTGGCACTGCCTAGTAAGGGAACTATGGAAAGGTTTGGATTTCTCCAA
ATCTGGGAGAATTTTCAAAATAAGAAAATAACCTTTATATGATATACTATGACTAGGCTG
TGTATTTCTTTTCAGGGATTTTTCTACCTTCAGGGTTGGATGTAGTTTAGTTACTATTAC
CATAGCCAACCTGTAGTTTTACATATACATTTTCTTGTGGAGCAATAGAGTTCTCCATTT
TACAGAAGCATTTTAAATGTAGTTTGAATATTTTCCACAAGATGCTGCAATGTGAGTTAT
CACTTCATTTATCTTAAAGAAAGACTAAACTGGTTGTCAGTTACATCTGACAGAAAAAAA
AAAAAAATCACTGTGTAACCAGGTTAAGTGGTAAAATAATCCAGGCGTCAGTCAAAGGCA
TTTTGCTGACTTTAATATTGATTATATTTTTAACAGGAATTTAAGAAAATATTACTGGAA
TTAAAAATATATATATATTAAACAAGAATTTTCTTTGCTCTGTCTAGCTTAAACTACTAC
TCAAGCTGCTTAAGTTCTTAAGTATTGTTTGTAATCACCAATAAATAAGTGCATTTGTAA
TTCATCAGTCATTATTAGCTTTTATTAAAAGAAGATTACGTTTTACAATGTAACTATAAT
CTCTTGAATTTGGTATCTTATTAATGAGTTTTAAAGATGTAAAACCTAACCTTTTTTAAA
GCTCCATTGTCTTATGTTTTTAGAGGCTTTTCCGTAAACATATATCTTACATATAATAAA
CTTTTCAAATCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MATFPCQLCGKTFLTLEKFTIHNYSHSRERPYKCVQPDCGKAFVSRYKLMRHMATHSPQK SHQCAHCEKTFNRKDHLKNHLQTHDPNKMAFGCEECGKKYNTMLGYKRHLALHAASSGDL TCGVCALELGSTEVLLDHLKAHAEEKPPSGTKEKKHQCDHCERCFYTRKDVRRHLVVHTG CKDFLCQFCAQRFGRKDHLTRHTKKTHSQELMKESLQTGDLLSTFHTISPSFQLKAAALP PFPLGASAQNGLASSLPAEVHSLTLSPPEQAAQPMQPLPESLASLHPSVSPGSPPPPLPN HKYNTTSTSYSPLASLPLKADTKGFCNISLFEDLPLQEPQSPQKLNPGFDLAKGNAGKVN LPKELPADAVNLTIPASLDLSPLLGFWQLPPPATQNTFGNSTLALGPGESLPHRLSCLGQ QQQEPPLAMGTVSLGQLPLPPIPHVFSAGTGSAILPHFHHAFR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
