Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00844051
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2709nts
Open reading frame: 138 to 2846
CTGGAGGGAGGCGGCAGCGACGGAGGAGGGGGCTTCTCAGAGAAAGGGAGGGAGGGAGCC
ACCCGGGTGAAGATACAGCAGCCTCCTGAACTCCCCCCTCCCACCCAGGCCGGGACCTGG
GGGCTCCTGCCGGATCCATGGGGGCGGCCAGCTGCGAGGATGAGGAGCTGGAATTTAAGC
TGGTGTTCGGGGAGGAAAAGGAGGCCCCCCCGCTGGGCGCGGGGGGATTGGGGGAAGAAC
TGGACTCAGAGGATGCCCCGCCATGCTGCCGTCTGGCCTTGGGAGAGCCCCCTCCCTATG
GCGCTGCACCTATCGGTATTCCCCGACCTCCACCCCCTCGGCCTGGCATGCATTCGCCAC
CGCCGCGACCAGCCCCCTCACCTGGCACCTGGGAGAGCCAGCCCGCCAGGTCGGTGAGGC
TGGGAGGACCAGGAGGGGGTGCTGGGGGTGCTGGGGGTGGCCGTGTTCTCGAGTGTCCCA
GCATCCGCATCACCTCCATCTCTCCCACGCCGGAGCCGCCAGCAGCGCTGGAGGACAACC
CTGATGCCTGGGGGGACGGCTCTCCTAGAGATTACCCCCCACCAGAAGGCTTTGGGGGCT
ACAGAGAAGCAGGGGGCCAGGGTGGGGGGGCCTTCTTCAGCCCAAGCCCTGGCAGCAGCA
GCCTGTCCTCGTGGAGCTTCTTCTCCGATGCCTCTGATGAGGCAGCCCTGTATGCAGCCT
GCGACGAGGTGGAGTCTGAGCTAAATGAGGCGGCCTCCCGCTTTGGCCTGGGCTCCCCGC
TGCCCTCGCCCCGGGCCTCCCCTCGGCCATGGACCCCCGAAGATCCCTGGAGCCTGTATG
GTCCAAGCCCCGGAGGCCGAGGGCCAGAGGATAGCTGGCTACTCCTCAGTGCTCCTGGGC
CCACCCCAGCCTCCCCGCGGCCTGCCTCTCCATGTGGCAAGCGGCGCTATTCCAGCTCGG
GAACCCCATCTTCAGCCTCCCCAGCTCTGTCCCGCCGTGGCAGCCTGGGGGAAGAGGGGT
CTGAGCCACCTCCACCACCCCCATTGCCTCTGGCCCGGGACCCGGGCTCCCCTGGTCCCT
TTGACTATGTGGGGGCCCCACCAGCTGAGAGCATCCCTCAGAAGACACGGCGGACTTCCA
GCGAGCAGGCAGTGGCTCTGCCTCGGTCTGAGGAGCCTGCCTCATGCAATGGGAAGCTGC
CCTTGGGAGCAGAGGAGTCTGTGGCTCCTCCAGGAGGTTCCCGGAAGGAGGTGGCTGGCA
TGGACTACCTGGCAGTGCCCTCCCCACTCGCTTGGTCCAAGGCCCGGATTGGGGGACACA
GCCCTATCTTCAGGACCTCTGCCCTACCCCCACTGGACTGGCCTCTGCCCAGCCAATATG
AGCAGCTGGAGCTGAGGATCGAGGTACAGCCTAGAGCCCACCACCGGGCCCACTATGAGA
CAGAAGGCAGCCGTGGAGCTGTCAAAGCTGCCCCTGGCGGTCACCCCGTAGTCAAGCTCC
TAGGCTACAGTGAGAAGCCACTGACCCTACAGATGTTCATCGGCACTGCAGATGAAAGGA
ACCTGCGGCCTCATGCCTTCTATCAGGTGCACCGTATCACAGGCAAGATGGTGGCCACGG
CCAGCTATGAAGCCGTAGTCAGTGGCACCAAGGTGTTGGAGATGACTCTGCTGCCTGAGA
ACAACATGGCGGCCAACATTGACTGCGCGGGAATCCTGAAGCTTCGGAATTCAGACATTG
AGCTTCGGAAGGGTGAGACGGACATCGGGCGCAAAAACACACGTGTACGGCTGGTGTTCC
GGGTACACGTGCCCCAGGGCGGCGGGAAGGTCGTCTCAGTACAGGCAGCATCGGTGCCCA
TCGAGTGCTCCCAGCGCTCAGCCCAGGAGCTGCCCCAGGTGGAGGCCTACAGCCCCAGTG
CCTGCTCTGTGAGAGGAGGCGAGGAACTGGTACTGACTGGCTCCAACTTCCTGCCAGACT
CCAAGGTGGTGTTCATTGAGAGGGGTCCTGATGGGAAGCTGCAATGGGAGGAGGAGGCCA
CAGTGAACCGACTGCAGAGCAACGAGGTGACGCTGACCCTGACTGTCCCCGAGTACAGCA
ACAAGAGGGTTTCCCGGCCAGTCCAGGTCTACTTTTATGTCTCCAATGGGCGGAGGAAAC
GCAGTCCTACCCAGAGTTTCAGGTTTCTGCCTGTGATCTGCAAAGAGGAGCCCCTACCGG
ACTCATCTCTGCGGGGTTTCCCTTCAGCATCGGCAACCCCCTTTGGCACTGACATGGACT
TCTCACCACCCAGGCCCCCCTACCCCTCCTATCCCCATGAAGACCCTGCTTGCGAAACTC
CTTACCTATCAGAAGGCTTCGGCTATGGCATGCCCCCTCTGTACCCCCAGACGGGGCCCC
CACCATCCTACAGACCGGGCCTGCGGATGTTCCCTGAGACTAGGGGTACCACAGGTTGTG
CCCAACCACCTGCAGTTTCCTTCCTTCCCCGCCCCTTCCCTAGTGACCCGTATGGAGGGC
GGGGCTCCTCTTTCTCCCTGGGGCTGCCATTCTCTCCGCCAGCCCCCTTTCGGCCGCCTC
CTCTTCCTGCATCCCCACCGCTTGAAGGCCCCTTCCCTTCCCAGAGTGATGTGCATCCCC
TACCTGCTGAGGGATACAATAAGGTAGGGCCAGGCTATGGCCCTGGGGAGGGGGCTCCGG
AGCAGGAGAAATCCAGGGGTGGCTACAGCAGCGGCTTCCGAGACAGTGTCCCTATCCAGG
GTATCACGCTGGAGGAAGTGAGTGAGATCATTGGCCGAGACCTGAGTGGCTTCCCTGCAC
CTCCTGGAGAAGAGCCTCCTGCCTGAACCACGTGAACTGTCATCACCTGGCAACCCCAGC
CCCAGCCTCAGCCCTTCCCCCTTTCCCTCCTTCCTGGAGTGGTGGCTACAGAAGCTTGGG
GCCAACCCTAGCTCCTCTTTCCCCAGCTTCTGTCTGTCTCACTGTCTTCCCTCCCCTCCC
CCAGCTGAGGTGTGGCCCTCAGGCCTGGTGCTGCCTTGGAGGGCTGGGGGAAGGAGTGTG
TGGAGGAGGGAGGAGGGTGAAGACTGAGGCTAGGTGCCAGAATGGACTGGAGTGAAGGCG
TGTCTAGAGTGTGGGCTGGCTGTTGTGCTGGAAAGCTGGGGACAGGTTGATGGTAATAAA
CTGCTCAATGACCAGTGCTTCAGGCTCCAGAGCTCTTTGGAGAGATGGGTTGGGGCAGCT
TACTCCAGCCCTGGCCCAAGGAGGCCCAGAAGTTGGAAAGAGATGGAATGTGGCTGGGAA
CATTGCATCCCAAAGAGCTTCTCAGTGGAGGAGGCTGGGGAAGGCATGAGGGGGCTCAGA
GGCTCCTTGACTGGGACCAGGATTGGGGGCCAGGGCTTGAGTAGGCCTCTCCACTCTCCT
CCTTGGGGGTCCAGATTCCTTAGGAGCTTTGGGATGAGGCCCAGGAGGCTGCATTTTTCC
AGGTCCTTAGTCTTGCCACCACACAGATGATTCTGATTCATAGCCAAGATGAGGACACAC
TGATGTAGCTGATCTCTCATTTACAGAGGAGGATTCTAAAGTTCAGAGAGGGAAAGGGGC
TTGCCTGAGGTCACGTAGATAATCAGCAGCACATTGAACGCTGCACTCCTGGGCTCCTGT
CCCCAGCCCCCATTCAGACACGCTGACTCAGGAGGTCCAGGCCTCTAAGGCTTCTCTCCC
TGGAGTGAGGGTGGAGGTGAGGGAGAGCTGGCACAAGCCCTCCCTCTGGATCCTCCACTC
CTGGGGATTATGAAGATATTCTGGAAAGATTTGTGCTTCAGAGGTAGACTGCAGAAAGCA
AACAGTCTACCCAGCAGCTCTGAATGTCACCTGCCCTGGGGCTTACAGCACTATATGAGT
TCCTGGCCTATCCTGCAAATATGCCCATGCTGGCCTTCTAAATAGCTGGTACATCCATCA
CCACTGACGGGCCTGGCCTGGAAACCTGGTTTGTCCCCTGTCTTGATGGCCTACGAGAGG
CCAAGTTCCACTGGGCTGGGAAAAGTCACTTTGTCTGTCTTGTTCACCTGGAGCCTGACA
CACCGTAGGTACTGAGTACAAATAGCTTGATTTGGCTAGGCTTGGCTGCAGGGGGACGTG
CCTAAAAGACATTCCGGGCATTTGCACTTGGGAAACTTGCCTCACCTTCAGGCTTGTGGG
GCCTCTCTATGCCCAGTGAGTCCAGGCAGTCCTAGCAAGTACTCAGGAGAGCAGGGGTGG
GTGTGACAGAGGCTGGCTCTGGATTGGGGGACAACAGAACCAGAGTAACTCCTCGCCTGT
TGCTGCTTTGCAATGAATTTCCTTTACCTTTCTGGAACACAAGCTGCTGTGAACCAAACT
GATATCAAGTGATTAGCTCACCGGGCCTTGGTTGCTTTTCAAAGATCCCCTTCAGCCCCC
TGCCAGAGTCACTGCCCCATAATCACCATGTCAGAAGGGACCCTAGGGCATTCGTGTCCT
ATTTATCAATCTTCAGCACCACCTCTAAGATCTCTGAGAGAGGGTGGATCAGCCTCTGTG
TAAACAAAAAGCTGTTAGGACTTGTTGCCTCTCAAGGTGGACTATTCTGTTTTCTGCCAG
GACACTGCCATTCATGCATTGTCAGATATTTATTAAACAGCAGCAAAGTGCCAGCCAATT
TGTCCTGGAGGAATTCATAGCCTCATGGGGCAAAAGTAAATAAACAGCTTATTACAATTC
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MGAASCEDEELEFKLVFGEEKEAPPLGAGGLGEELDSEDAPPCCRLALGEPPPYGAAPIG IPRPPPPRPGMHSPPPRPAPSPGTWESQPARSVRLGGPGGGAGGAGGGRVLECPSIRITS ISPTPEPPAALEDNPDAWGDGSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWS FFSDASDEAALYAACDEVESELNEAASRFGLGSPLPSPRASPRPWTPEDPWSLYGPSPGG RGPEDSWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSASPALSRRGSLGEEGSEPPPP PPLPLARDPGSPGPFDYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASCNGKLPLGAEE SVAPPGGSRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELR IEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERNLRPHA FYQVHRITGKMVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGE TDIGRKNTRVRLVFRVHVPQGGGKVVSVQAASVPIECSQRSAQELPQVEAYSPSACSVRG GEELVLTGSNFLPDSKVVFIERGPDGKLQWEEEATVNRLQSNEVTLTLTVPEYSNKRVSR PVQVYFYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRGFPSASATPFGTDMDFSPPRP PYPSYPHEDPACETPYLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRGTTGCAQPPAV SFLPRPFPSDPYGGRGSSFSLGLPFSPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGY NKVGPGYGPGEGAPEQEKSRGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPAPPGEEP PA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
