Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00846166
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4677nts
Open reading frame: 1 to 4677
ATGGAGCCGGGGTCCGACGATTTCCTACCGCCACCGGAGTGCCCGGTGTTCGAGCCTAGC
TGGGCCGAGTTCCGAGACCCTCTTGGCTACATCGCGAAAATCAGGCCCATCGCAGAGAAA
TCGGGCATTTGCAAGATCCGCCCACCCGCGGACTGGCAGCCACCCTTTGCTGTGGAAGTG
GACAACTTCAGGTTTACCCCCCGAATCCAGAGGCTGAATGAGCTAGAGGCCCAGACGAGA
GTGAAACTGAACTACTTGGACCAGATTGCCAAATTCTGGGAAATCCAGGGCTCCTCCTTA
AAGATTCCCAATGTAGAACGGCGGATCTTGGACCTCTACAGTCTCAGCAAAATTGTGGTG
GAGGAAGGTGGTTATGAAGCTATCTGCAAGGACCGTCGGTGGGCTCGGGTAGCCCAGCGC
CTCAACTATCCACCAGGCAAAAATATTGGCTCCTTGCTACGCTCCCACTACGAACGCATT
GTTTATCCCTATGAAATGTACCAGTCTGGAGCCAACCTTGTGTGTAACACACGTCCATTT
GATAATGAGGAGAAGGACAAGGAATACAAACCCCACAGCATCCCCCTACGACAGTCTGTG
CAGCCTTCCAAGTTCAACAGCTATGGCCGGCGGGCCAAGAGACTGCAGCCTGATCCGGAA
CCCACAGAGGAAGACATTGAGAAGAATCCAGAGCTGAAAAAGCTACAGATCTATGGGGCA
GGCCCCAAGATGATGGGCCTGGGCCTCATGGCCAAAGACAAGACTCTGCGGAAGAAAGAT
AAGGAGGGGCCTGAGTGTCCCCCCACAGTAGTGGTGAAGGAGGAGTTAGGTGGGGATGTG
AAGGTGGAGTCAACATCGCCTAAGACCTTCCTGGAGAGCAAGGAGGAGCTGAGTCACAGC
CCAGAACCCTGCACCAAGATGACCATGAGGCTACGGAGGAACCACAGCAATGCCCAGTTT
ATTGAGTCATATGTCTGCCGGATGTGTTCTCGAGGGGATGAGGATGACAAGCTCCTGCTG
TGTGATGGCTGTGATGACAACTACCACATCTTCTGCCTGCTGCCTCCTCTGCCTGAGATC
CCCAAGGGTGTCTGGCGGTGCCCAAAGTGTGTCATGGCGGAGTGTAAGCGGCCCCCAGAA
GCCTTTGGCTTTGAGCAGGCTACCCGGGAATACACTCTGCAGAGCTTTGGCGAGATGGCC
GACTCCTTTAAAGCTGACTACTTCAACATGCCCGTGCATATGGTGCCCACAGAACTTGTG
GAGAAGGAGTTCTGGAGGCTGGTAAATAGCATTGAGGAAGATGTGACTGTTGAGTATGGA
GCTGACATCCATTCCAAAGAATTTGGCAGCGGTTTCCCTGTCAGTGACAGTAAACGGCAC
CTAACCCCCGAAGAGGAGGAGTATGCTACCAGTGGTTGGAACCTAAATGTGATGCCGGTG
TTGGAACAGTCTGTACTGTGCCACATCAATGCAGATATCTCTGGCATGAAGGTGCCCTGG
CTCTACGTGGGCATGGTCTTCTCAGCCTTTTGCTGGCATATTGAGGATCACTGGAGTTAC
TCCATTAACTACCTCCACTGGGGTGAGCCGAAGACCTGGTATGGGGTGCCCTCACTTGCA
GCAGAACATTTGGAAGAAGTGATGAAGAAGCTGACACCTGAACTATTTGATAGCCAGCCT
GACCTCCTGCACCAACTTGTCACCCTCATGAATCCCAACACCCTCATGTCCCATGGTGTG
CCAGTTGTCCGCACAAACCAGTGTGCAGGAGAGTTTGTCATCACCTTCCCCCGTGCTTAC
CACAGCGGCTTCAACCAAGGCTACAACTTTGCCGAGGCTGTCAACTTTTGCACTGCTGAC
TGGTTGCCTGCTGGGCGCCAGTGCATTGAGCACTACCGCCGGCTCCGGAGATACTGCGTC
TTCTCCCATGAGGAGCTTATCTGCAAGATGGCTGCCTGCCCAGAGAAGCTAGACCTGAAC
CTGGCGGCAGCTGTGCATAAGGAGATGTTCATCATGGTGCAAGAAGAGCGGCGTCTACGA
AAGGCCCTGCTGGAGAAGGGTATCACAGAGGCTGAGCGAGAGGCTTTCGAGCTGCTCCCA
GATGATGAGCGCCAGTGTATCAAGTGCAAGACTACGTGTTTCCTGTCAGCCCTGGCCTGC
TACGACTGCCCAGACGGCCTTGTCTGCCTTTCCCACATCAATGATCTCTGCAAGTGCTCC
AGTAGCCGGCAGTACCTGCGGTATCGGTATACCTTGGATGAGCTTCCTGCCATGCTGCAT
AAGCTGAAGGTTCGGGCTGAGTCCTTTGACACCTGGGCCAACAAAGTGCGAGTGGCCCTG
GAGGTGGAGGATGGGCGGAAGCGCAGCCTTGAAGAACTGAGGGCACTAGAGTCTGAAGCC
CGTGAGCGGAGGTTTCCTAATAGTGAGCTGCTGCAGCAACTAAAGAACTGCCTGAGTGAG
GCAGAGGCTTGCGTGTCCCGAGCTCTGGGACTGGTCAGCGGCCAGGAAGCTGGCCCCCAC
AGGGTGGCTGGTCTACAGATGACCCTGACTGAGCTCCGGGCCTTTCTGGACCAGATGAAC
AACCTGCCTTGCGCCATGCACCAGATTGGGGATGTCAAGGGTGTTCTGGAACAGGTGGAG
GCCTACCAGGCTGAGGCTCGTGAGGCCCTGGCCTCACTGCCCTCCAGTCCAGGGCTACTG
CAGTCCCTGTTGGAGAGGGGGCGGCAGCTGGGGGTGGAGGTGCCTGAGGCCCAGCAGCTC
CAGCGGCAGGTGGAACAGGCGCGATGGCTGGATGAGGTGAAACGCACACTGGCCCCCTCA
GCCCGAAGGGGCACCTTGGCTGTCATGCGAGGACTGTTGGTCGCGGGTGCCAGTGTAGCC
CCTAGCCCTGCTGTGGATAAAGCCCAGGCCGAGCTGCAGGAACTGCTGACCATTGCTGAA
CGCTGGGAGGAGAAAGCCCACCTCTGCCTGGAGGCCAGGCAGAAGCATCCACCAGCCACA
CTTGAGGCCATAATCCGTGAAGCGGAAAACATCCCTGTTCACCTGCCCAACATCCAGGCT
CTCAAGGAGGCTCTTGCTAAGGCCCGGGCCTGGATTGCTGATGTTGATGAGATCCAAAAT
GGTGACCACTACCCCTGCCTGGATGACTTGGAGGGCCTAGTAGCTGTGGGCCGGGACCTA
CCTGTGGGGCTGGAGGAGCTGAGACAGCTAGAGCTACAGGTACTGACAGCGCACTCCTGG
AGGGAGAAGGCCTCCAAGACCTTCCTCAAGAAAAATTCTTGCTACACGCTGCTGGAGGTT
CTCTGCCCATGTGCAGATGCCGGCTCAGACAGCACCAAGCGCAGCCGGTGGATGGAGAAG
GAGCTGGGGTTGTACAAATCTGACACAGAGCTGCTGGGGCTGTCTGCGCAGGACCTCAGG
GACCCAGGCTCTGTGATCGTGGCCTTCAAGGAGGGGGAACAGAAGGAGAAGGAGGGTATC
CTGCAGCTGCGTCGCACCAATTCGGCCAAGCCCAGTCCACTGGCATCATCGAGCACGGCC
TCCTCTACAACCTCTATCTGTGTGTGTGGGCAGGTGCTGGCTGGGGCGGGAGCTCTGCAG
TGTGACCTGTGTCAGGACTGGTTCCATGGGCGGTGTGTGTCAGTGCCTCGCCTCCTCAGC
TCTCCGAGGCCCAATCCCACCTCATCCCCACTGCTGGCCTGGTGGGAATGGGACACCAAA
TTCCTGTGTCCACTGTGTATGCGCTCAAGGCGCCCGCGCCTGGAGACCATCCTGGCACTG
CTGGTAGCCCTGCAGAGACTGCCTGTGCGGCTGCCCGAGGGCGAGGCCCTGCAGTGCCTC
ACAGAGAGGGCCATCAGCTGGCAAGGCCGCGCCAGGCAGGCTCTGGCCTCTGAAGATGTG
ACTGCTCTTTTGGGACGGCTGGCTGAGCTCCGCCAACGGCTACAGGCTGAACCTAGACCT
GAGGAGCCTCCTAACTACCCTGCAGCCCCTGCTTCTGACCCCCTCAGAGAGGGCAGTGGC
AAGGATATGCCTAAGGTCCAGGGCTTACTGGAGAATGGAGACAGTGTGACCAGTCCTGAG
AAGGTAGCCCCGGAGGAGGGCTCAGGTAAGAGAGATCTGGAGCTGCTGTCCTCGCTGTTG
CCACAGTTGACTGGCCCTGTGTTGGAACTGCCTGAGGCAACCCGGGCCCCCTTGGAGGAG
CTCATGATGGAGGGGGACCTGCTCGAGGTGACCCTGGATGAGAACCACAGCATATGGCAG
CTGCTGCAGGCTGGACAGCCCCCAGACCTGGAGAGGATCCGCACACTTCTGGAGCTGGAG
AAGGCAGAGCGTCACGGGAGTCGGGCTCGGGGCCGGGCCCTGGAGAGGCGGCGGCGGCGG
AAGGTGGATCGGGGTGGGGAGGGCGATGACCCAGCCCGAGAGGAGCTAGAGCCAAAGAGG
GTACGGAGCTCAGGGCCAGAGGCTGAGGAGGTCCAGGAGGAGGAAGAGCTGGAGGAGGAG
ACTGGGGGTGAGGGCCCCCCTGCACCCATCCCCACCACTGGCAGCCCCAGCACCCAGGAG
AACCAGAATGGCTTGGAACCGGCGGAAGGGACCACTTCAGGCCCCTCGGCCCCTTTCTCC
ACTCTGACTCCCCGGCTGCATCTGCCCTGCCCACAGCAGCCGCCTCAGCAACAGTTG
Protein sequence predicted based on nucleotide sequence
MEPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEV DNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERRILDLYSLSKIVV EEGGYEAICKDRRWARVAQRLNYPPGKNIGSLLRSHYERIVYPYEMYQSGANLVCNTRPF DNEEKDKEYKPHSIPLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYGA GPKMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLESKEELSHS PEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLLCDGCDDNYHIFCLLPPLPEI PKGVWRCPKCVMAECKRPPEAFGFEQATREYTLQSFGEMADSFKADYFNMPVHMVPTELV EKEFWRLVNSIEEDVTVEYGADIHSKEFGSGFPVSDSKRHLTPEEEEYATSGWNLNVMPV LEQSVLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPSLA AEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPVVRTNQCAGEFVITFPRAY HSGFNQGYNFAEAVNFCTADWLPAGRQCIEHYRRLRRYCVFSHEELICKMAACPEKLDLN LAAAVHKEMFIMVQEERRLRKALLEKGITEAEREAFELLPDDERQCIKCKTTCFLSALAC YDCPDGLVCLSHINDLCKCSSSRQYLRYRYTLDELPAMLHKLKVRAESFDTWANKVRVAL EVEDGRKRSLEELRALESEARERRFPNSELLQQLKNCLSEAEACVSRALGLVSGQEAGPH RVAGLQMTLTELRAFLDQMNNLPCAMHQIGDVKGVLEQVEAYQAEAREALASLPSSPGLL QSLLERGRQLGVEVPEAQQLQRQVEQARWLDEVKRTLAPSARRGTLAVMRGLLVAGASVA PSPAVDKAQAELQELLTIAERWEEKAHLCLEARQKHPPATLEAIIREAENIPVHLPNIQA LKEALAKARAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEELRQLELQVLTAHSW REKASKTFLKKNSCYTLLEVLCPCADAGSDSTKRSRWMEKELGLYKSDTELLGLSAQDLR DPGSVIVAFKEGEQKEKEGILQLRRTNSAKPSPLASSSTASSTTSICVCGQVLAGAGALQ CDLCQDWFHGRCVSVPRLLSSPRPNPTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILAL LVALQRLPVRLPEGEALQCLTERAISWQGRARQALASEDVTALLGRLAELRQRLQAEPRP EEPPNYPAAPASDPLREGSGKDMPKVQGLLENGDSVTSPEKVAPEEGSGKRDLELLSSLL PQLTGPVLELPEATRAPLEELMMEGDLLEVTLDENHSIWQLLQAGQPPDLERIRTLLELE KAERHGSRARGRALERRRRRKVDRGGEGDDPAREELEPKRVRSSGPEAEEVQEEEELEEE TGGEGPPAPIPTTGSPSTQENQNGLEPAEGTTSGPSAPFSTLTPRLHLPCPQQPPQQQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
