Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00849211
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2859nts
Open reading frame: 1 to 2859
ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCA
CCATCTGAAATTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAA
GCTTTGAAGAAAGTAATCATTATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATG
ACCATCATTCGTTTTGTGCTACCTCTTCAGGATCACACTATCAAGAAATTACTTCTGGTA
TTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGACTTTTACATGAGATGATCCTT
GTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTCGAGGATCTACT
CTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCAGCTATT
CGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTAT
ACCATCTATAGAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTT
CTGGTGAATGAGAAGGATGCAAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCA
GATCAGGATCGAGCTTTGGATTACTTAAGTACTTGCATTGATCAAGTTCAAACATTTGGA
GACATTCTGCAGCTGGTTATTGTTGAACTGATTTATAAGGTCTGTCATGCTAATCCATCA
GAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTACAGTCATCCAGCCCTGCTGTA
AAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACTGCAATCAAGGCT
GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTCATA
GTTTTGGATCGCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGAT
CTGGTTATGGATATCCTAAGAGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACT
CTGCAGTTAGCACTGGATCTTGTCTCTTCTAGAAATGTTGAAGAGCTGGTTATTGTCCTG
AAGAAGGAAGTGATAAAAACAAATAATGTGTCTGAGCATGAAGATACTGACAAATACAGA
CAACTCCTAGTGCGAACATTGCATTCCTGTTCTGTCCGATTTCCAGATATGGCTGCAAAT
GTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAAGCAGCAGCTGCTGATGTC
TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTTATTGTTGAG
AAGATGCTTGAAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGG
ATCCTGGGAGAATACTGTAGTACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGC
AGGTCCCTTGGAGAGATCCCAATTGTAGAGTCAGAAATAAAGAAAGAAGCTGGTGAATTA
AAACCTGAAGAAGAAATAACTGTAGGGCCAGTTCAGAAATTGGTTACTGAAATGGGTACC
TATGCAACTCAGAGTGCCCTTAGCAGTTCTAGACCCACCAAGAAAGAGGAAGACAGACCT
CCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTTGTTGCTGCCTCCCTTGCCACAACT
CTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAGAAAAAGCAAAATTCT
TTTGTTGCTGAGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCCTCTCTT
CCTAAGAAGCCAATTACTGATGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTG
TCTGAATGTTCACCTTTAATGAATGACATTTTCAATAAGGAATGCAGACAGTCCCTTTCT
CACATGTTATCTGCTAAACTAGAAGAAGAGAAATTATCCCAAAAGAAAGAATCTGAAAAG
AGGAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAACTGCTAAGAAT
GAAATGAACTGCAAGGAAGATCAGTTTCAGCTGAGTTTACTGGCAGCAATGGGTAACACA
CAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTTAACAAGGTCACCCAATTGACA
GGTTTCTCAGATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAATATGATATTGTC
CTGGATGTACTTGTTGTGAACCAAACCAGTGATACTTTGCAGAATTGCACATTAGAACTA
GCTACACTAGGGGATCTGAAACTTGTGGAAAAGCCGTCTCCTTTGACTCTTGCTCCTCAT
GACTTCGCAAATATTAAAGCTAACGTCAAAGTAGCATCAACAGAAAATGGAATAATTTTT
GGTAATATAGTTTATGATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGTGGTTCTCAGT
GATATTCACATCGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCAGAATTC
CGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGTGACAGTTAACACCAACATGGTT
GATTTAAATGACTACTTACAGCACATATTAAAGTCAACCAATATGAAATGCCTGACTCCA
GAAAAGGCCCTTTCTGGTTACTGTGGCTTTATGGCAGCCAACCTTTATGCTCGTTCCATA
TTTGGTGAAGATGCACTTGCAAATGTCAGCATTGAGAAGCCAATTCACCAGGGACCAGAT
GCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCCAGGGAATGGCCTTAAGTCTT
GGAGATAAAATCAACTTGTCACAGAAGAAAACTAGTATA
Protein sequence predicted based on nucleotide sequence
MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLM TIIRFVLPLQDHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGST LRFLCKLKEAELLEPLMPAIRACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDF LVNEKDASCKRNAFMMLIHADQDRALDYLSTCIDQVQTFGDILQLVIVELIYKVCHANPS ERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKAAAQCYIDLIIKESDNNVKLI VLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSSRNVEELVIVL KKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADV LEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIR RSLGEIPIVESEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRP PLRGFLLDGDFFVAASLATTLTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSL PKKPITDDDVDRISLCLKVLSECSPLMNDIFNKECRQSLSHMLSAKLEEEKLSQKKESEK RNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNTQRKEAADPLASKLNKVTQLT GFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVEKPSPLTLAPH DFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEF RQMWAEFEWENKVTVNTNMVDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSI FGEDALANVSIEKPIHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
