Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850675
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3474nts
Open reading frame: 1 to 3474
ATGGAGTCAGGCCACCTCCTCTGGGCTCTGCTGTTCATGCAGTCCTTGTGGCCTCAACTG
ACTGATGGAGCCACTCGAGTCTACTACCTGGGCATCCGGGATGTGCAGTGGAACTATGCT
CCCAAGGGAAGAAATGTCATCACGAACCAGCCTCTGGACAGTGACATAGTGGCTTCCAGC
TTCTTAAAGTCTGACAAGAACCGGATAGGGGGAACCTACAAGAAGACCATCTATAAAGAA
TACAAGGATGACTCATACACAGATGAAGTGGCCCAGCCTGCCTGGTTGGGCTTCCTGGGG
CCAGTGTTGCAGGCTGAAGTGGGGGATGTCATTCTTATTCACCTGAAGAATTTTGCCACT
CGTCCCTATACCATCCACCCTCATGGTGTCTTCTACGAGAAGGACTCTGAAGGTTCCCTA
TACCCAGATGGCTCCTCTGGGCCACTGAAAGCTGATGACTCTGTTCCCCCGGGGGGCAGC
CATATCTACAACTGGACCATTCCAGAAGGCCATGCACCCACCGATGCTGACCCAGCGTGC
CTCACCTGGATCTACCATTCTCATGTAGATGCTCCACGAGACATTGCAACTGGCCTAATT
GGGCCTCTCATCACCTGTAAAAGAGGAGCCCTGGATGGGAACTCCCCTCCTCAACGCCAG
GATGTAGACCATGATTTCTTCCTCCTCTTCAGTGTGGTAGATGAGAACCTCAGCTGGCAT
CTCAATGAGAACATTGCCACTTACTGCTCAGATCCTGCTTCAGTGGACAAAGAAGATGAG
ACATTTCAGGAGAGCAATAGGATGCATGCAATCAATGGCTTTGTTTTTGGGAATTTACCT
GAGCTGAACATGTGTGCACAGAAACGTGTGGCCTGGCACTTGTTTGGCATGGGCAATGAA
ATTGATGTCCACACAGCATTTTTCCATGGACAGATGCTGACTACCCGTGGACACCACACT
GATGTGGCTAACATCTTTCCAGCCACCTTTGTGACTGCTGAGATGGTGCCCTGGGAACCT
GGTACCTGGTTAATTAGCTGCCAAGTGAACAGTCACTTTCGAGATGGCATGCAGGCACTC
TACAAGGTCAAGTCTTGCTCCATGGCCCCTCCTGTGGACCTGCTCACAGGCAAAGTTCGA
CAGTACTTCATTGAGGCCCATGAGATTCAATGGGACTATGGCCCGATGGGGCATGATGGG
AGTACTGGGAAGAATTTGAGAGAGCCAGGCAGTATCTCAGATAAGTTTTTCCAGAAGAGC
TCCAGCCGAATTGGGGGCACTTACTGGAAAGTGCGATATGAAGCCTTTCAAGATGAGACA
TTCCAAGAGAAGATGCATTTGGAGGAAGATAGGCATCTTGGAATCCTGGGGCCAGTGATC
CGGGCTGAGGTGGGTGACACCATTCAGGTGGTCTTCTACAACCGTGCCTCCCAGCCATTC
AGCATGCAGCCCCATGGGGTCTTTTATGAGAAAGACTATGAAGGCACTGTGTACAATGAT
GGCTCATCTTACCCTGGCTTGGTTGCCAAGCCCTTTGAGAAAGTAACATACCGCTGGACA
GTCCCCCCTCATGCCGGTCCCACTGCTCAGGATCCTGCTTGTCTCACTTGGATGTACTTC
TCTGCTGCAGATCCCATAAGAGACACAAATTCTGGCCTGGTGGGCCCGCTGCTGGTGTGC
AGGGCTGGTGCCTTGGGTGCAGATGGCAAGCAGAAAGGGGTGGATAAAGAATTCTTTCTT
CTCTTCACTGTGTTGGATGAGAACAAGAGCTGGTACAGCAATGCCAATCAAGCAGCTGCT
ATGTTGGATTTCCGACTGCTTTCAGAGGATATTGAGGGCTTCCAAGACTCCAATCGGATG
CATGCCATTAATGGGTTTCTGTTCTCTAACCTGCCCAGGCTGGACATGTGCAAGGGTGAC
ACAGTGGCCTGGCACCTGCTCGGCCTGGGCACAGAGACTGATGTGCATGGAGTCATGTTC
CAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAGGGTGCAGCTATGCTCTTTCCTCAT
ACCTTTGTCATGGCCATCATGCAGCCTGACAACCTTGGGACATTTGAGATTTATTGCCAG
GCAGGCAGCCATCGAGAAGCAGGGATGAGGGCAATCTATAATGTCTCCCAGTGTCCTGGC
CACCAAGCCACCCCTCGCCAACGCTACCAAGCTGCAAGAATCTACTATATCATGGCAGAA
GAAGTAGAGTGGGACTATTGCCCTGACCGGAGCTGGGAACGGGAATGGCACAACCAGTCT
GAGAAGGACAGTTATGGTTACATTTTCCTGAGCAACAAGGATGGGCTCCTGGGTTCCAGA
TACAAGAAAGCTGTATTCAGGGAATACACTGATGGTACATTCAGGATCCCTCGGCCAAGG
ACTGGACCAGAAGAACACTTGGGAATCTTGGGTCCACTTATCAAAGGTGAAGTTGGTGAT
ATCCTGACTGTGGTATTCAAGAATAATGCCAGCCGCCCCTACTCTGTGCATGCTCATGGA
GTGCTAGAATCTACTACTGTCTGGCCACTGGCTGCTGAGCCTGGTGAGGTGGTCACTTAT
CAGTGGAACATCCCAGAGAGGTCTGGCCCTGGGCCCAATGACTCTGCTTGTGTTTCCTGG
ATCTATTATTCTGCAGTGGATCCCATCAAGGACATGTATAGTGGCCTGGTGGGGCCCTTG
GCTATCTGCCAAAAGGGCATCCTGGAGCCCCATGGAGGACGGAGTGACATGGATCGGGAA
TTTGCATTGTTGTTCTTGATTTTTGATGAAAATAAGTCTTGGTATTTGGAGGAAAATGTG
GCAACCCATGGGTCCCAGGATCCAGGCAGTATTAACCTACAGGATGAAACTTTCTTGGAG
AGCAATAAAATGCATGCAATCAATGGGAAACTCTATGCCAACCTTAGGGGTCTTACCATG
TACCAAGGAGAACGAGTGGCCTGGTACATGCTGGCCATGGGCCAAGATGTGGATCTACAC
ACCATCCACTTTCATGCAGAGAGCTTCCTCTATCGGAATGGCGAGAACTACCGGGCAGAT
GTGGTGGATCTGTTCCCAGGGACTTTTGAGGTTGTGGAGATGGTGGCCAGCAACCCTGGG
ACATGGCTGATGCACTGCCATGTGACTGACCATGTCCATGCTGGCATGGAGACCCTCTTC
ACTGTTTTTTCTCGAACAGAACACTTAAGCCCTCTCACCGTCATCACCAAAGAGACTGAA
AAAGCAGTGCCCCCCAGAGACATTGAAGAAGGCAATGTGAAGATGCTGGGCATGCAGATC
CCCATAAAGAATGTTGAGATGCTGGCCTCTGTTTTGGTTGCCATTAGTGTCACCCTTCTG
CTCGTTGTTCTGGCTCTTGGTGGAGTGGTTTGGTACCAACATCGACAGAGAAAGCTACGA
CGCAATAGGAGGTCCATCCTGGATGACAGCTTCAAGCTTCTGTCTTTCAAACAG
Protein sequence predicted based on nucleotide sequence
MESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASS FLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFAT RPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPAC LTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWH LNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNE IDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQAL YKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKS SSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPF SMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYF SAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAA MLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMF QGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPG HQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSR YKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHG VLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPL AICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLE SNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRAD VVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETE KAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLR RNRRSILDDSFKLLSFKQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
