Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850855
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3543nts
Open reading frame: 1 to 3543
ATGGGGCCAGAACGGACAGGGGCCGCGCCGCTGCCGCTGCTGCTGGTGTTAGCGCTCAGT
CAAGGCATTTTAAATTGTTGTTTGGCCTACAATGTTGGTCTCCCAGAAGCAAAAATATTT
TCCGGTCCTTCAAGTGAACAGTTTGGCTATGCAGTGCAGCAGTTTATAAATCCAAAAGGC
AACTGGTTACTGGTTGGTTCACCCTGGAGTGGCTTTCCTGAGAACCGAATGGGAGATGTG
TATAAATGTCCTGTTGACCTATCCACTGCCACATGTGAAAAACTAAATTTGCAAACTTCA
ACAAGCATTCCAAATGTTACTGAGATGAAAACCAACATGAGCCTCGGCTTGATCCTCACC
AGGAACATGGGAACTGGAGGTTTTCTCACATGTGGTCCTCTGTGGGCACAGCAATGTGGG
AATCAGTATTACACAACGGGTGTGTGTTCTGACATCAGTCCTGATTTTCAGCTCTCAGCC
AGCTTCTCACCTGCAACTCAGCCCTGCCCTTCCCTCATAGATGTTGTGGTTGTGTGTGAT
GAATCAAATAGTATTTATCCTTGGGATGCAGTAAAGAATTTTTTGGAAAAATTTGTACAA
GGCCTGGATATAGGCCCCACAAAGACACAGGTGGGGTTAATTCAGTATGCCAATAATCCA
AGAGTTGTGTTTAACTTGAACACATATAAAACCAAAGAAGAAATGATTGTAGCAACATCC
CAGACATCCCAATATGGTGGGGACCTCACAAACACATTCGGAGCAATTCAATATGCAAGA
AAATATGCTTATTCAGCAGCTTCTGGTGGGCGACGAAGTGCTACGAAAGTAATGGTAGTT
GTAACTGACGGTGAATCACATGATGGTTCAATGTTGAAAGCTGTGATTGATCAATGCAAC
CATGACAATATACTGAGGTTTGGCATAGCAGTTCTTGGGTACTTAAACAGAAACGCCCTT
GATACTAAAAATTTAATAAAAGAAATAAAAGCAATCGCTAGTATTCCAACAGAAAGATAC
TTTTTCAATGTGTCTGATGAAGCAGCTCTACTAGAAAAGGCTGGGACATTAGGAGAACAA
ATTTTCAGCATTGAAGGTACTGTTCAAGGAGGAGACAACTTTCAGATGGAAATGTCACAA
GTGGGATTCAGTGCAGATTACTCTTCTCAAAATGATATTCTGATGCTGGGTGCAGTGGGA
GCTTTTGGCTGGAGTGGGACCATTGTCCAGAAGACATCTCATGGCCATTTGATCTTTCCT
AAACAAGCCTTTGACCAAATTCTGCAGGACAGAAATCACAGTTCATATTTAGGTTACTCT
GTGGCTGCAATTTCTACTGGAGAAAGCACTCACTTTGTTGCTGGTGCTCCTCGGGCAAAT
TATACCGGCCAGATAGTGCTATATAGTGTGAATGAGAATGGCAATATCACGGTTATTCAG
GCTCACCGAGGTGACCAGATTGGCTCCTATTTTGGTAGTGTGCTGTGTTCAGTTGATGTG
GATAAAGACACCATTACAGACGTGCTCTTGGTAGGTGCACCAATGTACATGAGTGACCTA
AAGAAAGAGGAAGGAAGAGTCTACCTGTTTACTATCAAAGAGGGCATTTTGGGTCAGCAC
CAATTTCTTGAAGGCCCCGAGGGCATTGAAAACACTCGATTTGGTTCAGCAATTGCAGCT
CTTTCAGACATCAACATGGATGGCTTTAATGATGTGATTGTTGGTTCACCACTAGAAAAT
CAGAATTCTGGAGCTGTATACATTTACAATGGTCATCAGGGCACTATCCGCACAAAGTAT
TCCCAGAAAATCTTGGGATCCGATGGAGCCTTTAGGAGCCATCTCCAGTACTTTGGGAGG
TCCTTGGATGGCTATGGAGATTTAAATGGGGATTCCATCACCGATGTGTCTATTGGTGCC
TTTGGACAAGTGGTTCAACTCTGGTCACAAAGTATTGCTGATGTAGCTATAGAAGCTTCA
TTCACACCAGAAAAAATCACTTTGGTCAACAAGAATGCTCAGATAATTCTCAAACTCTGC
TTCAGTGCAAAGTTCAGACCTACTAAGCAAAACAATCAAGTGGCCATTGTATATAACATC
ACACTTGATGCAGATGGATTTTCATCCAGAGTAACCTCCAGGGGGTTATTTAAAGAAAAC
AATGAAAGGTGCCTGCAGAAGAATATGGTAGTAAATCAAGCACAGAGTTGCCCCGAGCAC
ATCATTTATATACAGGAGCCCTCTGATGTTGTCAACTCTTTGGATTTGCGTGTGGACATC
AGTCTGGAAAACCCTGGCACTAGCCCTGCCCTTGAAGCCTATTCTGAGACTGCCAAGGTC
TTCAGTATTCCTTTCCACAAAGACTGTGGTGAGGACGGACTTTGCATTTCTGATCTAGTC
CTAGATGTCCGACAAATACCAGCTGCTCAAGAACAACCCTTTATTGTCAGCAACCAAAAC
AAAAGGTTAACATTTTCAGTAACGCTGAAAAATAAAAGGGAAAGTGCATACAACACTGGA
ATTGTTGTTGATTTTTCAGAAAACTTGTTTTTTGCATCATTCTCCCTGCCGGTTGATGGG
ACAGAAGTAACATGCCAGGTGGCTGCATCTCAGAAGTCTGTTGCCTGCGATGTAGGCTAC
CCTGCTTTAAAGAGAGAACAACAGGTGACTTTTACTATTAACTTTGACTTCAATCTTCAA
AACCTTCAGAATCAGGCGTCTCTCAGTTTCCAAGCCTTAAGTGAAAGCCAAGAAGAAAAC
AAGGCTGATAATTTGGTCAACCTCAAAATTCCTCTCCTGTATGATGCTGAAATTCACTTA
ACAAGATCTACCAACATAAATTTTTATGAAATCTCTTCGGATGGGAATGTTCCTTCAATC
GTGCACAGTTTTGAAGATGTTGGTCCAAAATTCATCTTCTCCCTGAAGGTAACAACAGGA
AGTGTTCCAGTAAGCATGGCAACTGTAATCATCCACATCCCTCAGTATACCAAAGAAAAG
AACCCACTGATGTACCTAACTGGGGTGCAAACAGACAAGGCTGGTGACATCAGTTGTAAT
GCAGATATCAATCCACTGAAAATAGGACAAACATCTTCTTCTGTATCTTTCAAAAGTGAA
AATTTCAGGCACACCAAAGAATTGAACTGCAGAACTGCTTCCTGTAGTAATGTTACCTGC
TGGTTGAAAGACGTTCACATGAAAGGAGAATACTTTGTTAATGTGACTACCAGAATTTGG
AACGGGACTTTCGCATCATCAACGTTCCAGACAGTACAGCTAACGGCAGCTGCAGAAATC
AACACCTATAACCCTGAGATATATGTGATTGAAGATAACACTGTTACGATTCCCCTGATG
ATAATGAAACCTGATGAGAAAGCCGAAGTACCAACAGGAGTTATAATAGGAAGTATAATT
GCTGGAATCCTTTTGCTGTTAGCTCTGGTTGCAATTTTATGGAAGCTCGGCTTCTTCAAA
AGAAAATATGAAAAGATGACCAAAAATCCAGATGAGATTGATGAGACCACAGAGCTCAGT
AGC
Protein sequence predicted based on nucleotide sequence
MGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKG NWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILT RNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASFSPATQPCPSLIDVVVVCD ESNSIYPWDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATS QTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVTDGESHDGSMLKAVIDQCN HDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQ IFSIEGTVQGGDNFQMEMSQVGFSADYSSQNDILMLGAVGAFGWSGTIVQKTSHGHLIFP KQAFDQILQDRNHSSYLGYSVAAISTGESTHFVAGAPRANYTGQIVLYSVNENGNITVIQ AHRGDQIGSYFGSVLCSVDVDKDTITDVLLVGAPMYMSDLKKEEGRVYLFTIKEGILGQH QFLEGPEGIENTRFGSAIAALSDINMDGFNDVIVGSPLENQNSGAVYIYNGHQGTIRTKY SQKILGSDGAFRSHLQYFGRSLDGYGDLNGDSITDVSIGAFGQVVQLWSQSIADVAIEAS FTPEKITLVNKNAQIILKLCFSAKFRPTKQNNQVAIVYNITLDADGFSSRVTSRGLFKEN NERCLQKNMVVNQAQSCPEHIIYIQEPSDVVNSLDLRVDISLENPGTSPALEAYSETAKV FSIPFHKDCGEDGLCISDLVLDVRQIPAAQEQPFIVSNQNKRLTFSVTLKNKRESAYNTG IVVDFSENLFFASFSLPVDGTEVTCQVAASQKSVACDVGYPALKREQQVTFTINFDFNLQ NLQNQASLSFQALSESQEENKADNLVNLKIPLLYDAEIHLTRSTNINFYEISSDGNVPSI VHSFEDVGPKFIFSLKVTTGSVPVSMATVIIHIPQYTKEKNPLMYLTGVQTDKAGDISCN ADINPLKIGQTSSSVSFKSENFRHTKELNCRTASCSNVTCWLKDVHMKGEYFVNVTTRIW NGTFASSTFQTVQLTAAAEINTYNPEIYVIEDNTVTIPLMIMKPDEKAEVPTGVIIGSII AGILLLLALVAILWKLGFFKRKYEKMTKNPDEIDETTELSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
