Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850866
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3678nts
Open reading frame: 1 to 3678
ATGGCTCCACTCCGCGCGCTGCTGTCCTACCTGCTGCCTTTGCACTGTGCGCTCTGCGCC
GCCGCGGGCAGCCGGACCCCAGAGCTGCACCTCTCTGGAAAGCTCAGTGACTATGGTGTG
ACAGTGCCCTGCAGCACAGACTTTCGGGGACGCTTCCTCTCCCACGTGGTGTCTGGCCCA
GCAGCAGCCTCTGCAGGGAGCATGGTAGTGGACACGCCACCCACACTACCACGACACTCC
AGTCACCTCCGGGTGGCTCGCAGCCCTCTGCACCCAGGAGGGACCCTGTGGCCTGGCAGG
GTGGGGCGCCACTCCCTCTACTTCAATGTCACTGTTTTCGGGAAGGAACTGCACTTGCGC
CTGCGGCCCAATCGGAGGTTGGTAGTGCCAGGATCCTCAGTGGAGTGGCAGGAGGATTTT
CGGGAGCTGTTCCGGCAGCCCTTACGGCAGGAGTGTGTGTACACTGGAGGTGTCACTGGA
ATGCCTGGGGCAGCTGTTGCCATCAGCAACTGTGACGGATTGGCGGGCCTCATCCGCACA
GACAGCACCGACTTCTTCATTGAGCCTCTGGAGCGGGGCCAGCAGGAGAAGGAGGCCAGC
GGGAGGACACATGTGGTGTACCGCCGGGAGGCCGTCCAGCAGGAGTGGGCAGAACCTGAC
GGGGACCTGCACAATGAAGCCTTTGGCCTGGGAGACCTTCCCAACCTGCTGGGCCTGGTG
GGGGACCAGCTGGGCGACACAGAGCGGAAGCGGCGGCATGCCAAGCCAGGCAGCTACAGC
ATCGAGGTGCTGCTGGTGGTGGACGACTCGGTGGTTCGCTTCCATGGCAAGGAGCATGTG
CAGAACTATGTCCTCACCCTCATGAATATCGTAGATGAGATTTACCACGATGAGTCCCTG
GGGGTTCATATAAATATTGCCCTCGTCCGCTTGATCATGGTTGGCTACCGACAGTCCCTG
AGCCTGATCGAGCGCGGGAACCCCTCACGCAGCCTGGAGCAGGTGTGTCGCTGGGCACAC
TCCCAGCAGCGCCAGGACCCCAGCCACGCTGAGCACCATGACCACGTTGTGTTCCTCACC
CGGCAGGACTTTGGGCCCTCAGGTATGCAAGGGTATGCACCCGTCACTGGCATGTGTCAC
CCCCTGAGGAGCTGTGCCCTCAACCATGAGGATGGCTTCTCCTCAGCCTTCGTGATAGCT
CATGAGACCGGCCACGTGCTCGGCATGGAGCATGACGGTCAGGGGAATGGCTGTGCAGAT
GAGACCAGCCTGGGCAGCGTCATGGCGCCCCTGGTGCAGGCTGCCTTCCACCGCTTCCAT
TGGTCCCGCTGCAGCAAGCTGGAGCTCAGCCGCTACCTCCCCTCCTACGACTGCCTCCTC
GATGACCCCTTTGATCCTGCCTGGCCCCAGCCCCCAGAGCTGCCTGGGATCAACTACTCA
ATGGATGAGCAGTGCCGCTTTGACTTTGGCAGTGGCTACCAGACCTGCTTGGCATTCAGG
ACCTTTGAGCCCTGCAAGCAGCTGTGGTGCAGCCATCCTGACAACCCGTACTTCTGCAAG
ACCAAGAAGGGGCCCCCGCTGGATGGGACTGAGTGTGCACCCGGCAAGTGGTGCTTCAAA
GGTCACTGCATCTGGAAGTCGCCGGAGCAGACATATGGCCAGGATGGAGGCTGGAGCTCC
TGGACCAAGTTTGGGTCATGTTCGCGGTCATGTGGGGGCGGGGTGCGATCCCGCAGCCGG
AGCTGCAACAACCCCTCCCCAGCCTATGGAGGCCGCCTGTGCTTAGGGCCCATGTTCGAG
TACCAGGTCTGCAACAGCGAGGAGTGCCCTGGGACCTACGAGGACTTCCGGGCCCAGCAG
TGTGCCAAGCGCAACTCCTACTATGTGCACCAGAATGCCAAGCACAGCTGGGTGCCCTAC
GAGCCTGACGATGACGCCCAGAAGTGTGAGCTGATCTGCCAGTCGGCGGACACGGGGGAC
GTGGTGTTCATGAACCAGGTGGTTCACGATGGGACACGCTGCAGCTACCGGGACCCATAC
AGCGTCTGTGCGCGTGGCGAGTGTGTGCCTGTCGGCTGTGACAAGGAGGTGGGGTCCATG
AAGGCGGATGACAAGTGTGGAGTCTGCGGGGGTGACAACTCCCACTGCAGGACTGTGAAG
GGGACGCTGGGCAAGGCCTCCAAGCAGGCAGGAGCTCTCAAGCTGGTGCAGATCCCAGCA
GGTGCCAGGCACATCCAGATTGAGGCACTGGAGAAGTCCCCCCACCGCATTGTGGTGAAG
AACCAGGTCACCGGCAGCTTCATCCTCAACCCCAAGGGCAAGGAAGCCACAAGCCGGACC
TTCACCGCCATGGGCCTGGAGTGGGAGGATGCGGTGGAGGATGCCAAGGAAAGCCTCAAG
ACCAGCGGGCCCCTGCCTGAAGCCATTGCCATCCTGGCTCTCCCCCCAACTGAGGGTGGC
CCCCGCAGCAGCCTGGCCTACAAGTACGTCATCCATGAGGACCTGCTGCCCCTTATCGGG
AGCAACAATGTGCTCCTGGAGGAGATGGACACCTATGAGTGGGCGCTCAAGAGCTGGGCC
CCCTGCAGCAAGGCCTGTGGAGGAGGGATCCAGTTCACCAAATACGGCTGCCGGCGCAGA
CGAGACCACCACATGGTGCAGCGACACCTGTGTGACCACAAGAAGAGGCCCAAGCCCATC
CGCCGGCGCTGCAACCAGCACCCGTGCTCTCAGCCTGTGTGGGTGACGGAGGAGTGGGGT
GCCTGCAGCCGGAGCTGTGGGAAGCTGGGGGTGCAGACACGGGGGATACAGTGCCTGCTG
CCCCTCTCCAATGGAACCCACAAGGTCATGCCGGCCAAAGCCTGCGCCGGGGACCGGCCT
GAGGCCCGACGGCCCTGTCTCCGAGTGCCCTGCCCAGCCCAGTGGAGGCTGGGAGCCTGG
TCCCAGTGCTCTGCCACCTGTGGAGAGGGCATCCAGCAGCGGCAGGTGGTGTGCAGGACC
AACGCCAACAGCCTCGGGCATTGCGAGGGGGATAGGCCAGACACTGTCCAGGTCTGCAGC
CTGCCCGCCTGTGGAGGAAATCACCAGAACTCCACGGTGAGGGCCGATGTCTGGGAACTT
GGGACGCCAGAGGGGCAGTGGGTGCCACAATCTGAACCCCTACATCCCATTAACAAGATA
TCATCAACGGAGCCCTGCACGGGAGACAGGTCTGTCTTCTGCCAGATGGAAGTGCTCGAT
CGCTACTGCTCCATTCCCGGCTACCACCGGCTCTGCTGTGTGTCCTGCATCAAGAAGGCC
TCGGGCCCCAACCCTGGCCCAGACCCTGGCCCAACCTCACTGCCCCCCTTCTCCACTCCT
GGAAGCCCCTTACCAGGACCCCAGGACCCTGCAGATGCTGCAGAGCCTCCTGGAAAGCCA
ACGGGATCAGAGGACCATCAGCATGGCCGAGCCACACAGCTCCCAGGAGCTCTGGATACA
AGCTCCCCAGGGACCCAGCATCCCTTTGCCCCTGAGACACCAATCCCTGGAGCATCCTGG
AGCATCTCCCCTACCACCCCCGGGGGGCTGCCTTGGGGCTGGACTCAGACACCTACGCCA
GTCCCTGAGGACAAAGGGCAACCTGGAGAAGACCTGAGACATCCCGGCACCAGCCTCCCT
GCTGCCTCCCCGGTGACA
Protein sequence predicted based on nucleotide sequence
MAPLRALLSYLLPLHCALCAAAGSRTPELHLSGKLSDYGVTVPCSTDFRGRFLSHVVSGP AAASAGSMVVDTPPTLPRHSSHLRVARSPLHPGGTLWPGRVGRHSLYFNVTVFGKELHLR LRPNRRLVVPGSSVEWQEDFRELFRQPLRQECVYTGGVTGMPGAAVAISNCDGLAGLIRT DSTDFFIEPLERGQQEKEASGRTHVVYRREAVQQEWAEPDGDLHNEAFGLGDLPNLLGLV GDQLGDTERKRRHAKPGSYSIEVLLVVDDSVVRFHGKEHVQNYVLTLMNIVDEIYHDESL GVHINIALVRLIMVGYRQSLSLIERGNPSRSLEQVCRWAHSQQRQDPSHAEHHDHVVFLT RQDFGPSGMQGYAPVTGMCHPLRSCALNHEDGFSSAFVIAHETGHVLGMEHDGQGNGCAD ETSLGSVMAPLVQAAFHRFHWSRCSKLELSRYLPSYDCLLDDPFDPAWPQPPELPGINYS MDEQCRFDFGSGYQTCLAFRTFEPCKQLWCSHPDNPYFCKTKKGPPLDGTECAPGKWCFK GHCIWKSPEQTYGQDGGWSSWTKFGSCSRSCGGGVRSRSRSCNNPSPAYGGRLCLGPMFE YQVCNSEECPGTYEDFRAQQCAKRNSYYVHQNAKHSWVPYEPDDDAQKCELICQSADTGD VVFMNQVVHDGTRCSYRDPYSVCARGECVPVGCDKEVGSMKADDKCGVCGGDNSHCRTVK GTLGKASKQAGALKLVQIPAGARHIQIEALEKSPHRIVVKNQVTGSFILNPKGKEATSRT FTAMGLEWEDAVEDAKESLKTSGPLPEAIAILALPPTEGGPRSSLAYKYVIHEDLLPLIG SNNVLLEEMDTYEWALKSWAPCSKACGGGIQFTKYGCRRRRDHHMVQRHLCDHKKRPKPI RRRCNQHPCSQPVWVTEEWGACSRSCGKLGVQTRGIQCLLPLSNGTHKVMPAKACAGDRP EARRPCLRVPCPAQWRLGAWSQCSATCGEGIQQRQVVCRTNANSLGHCEGDRPDTVQVCS LPACGGNHQNSTVRADVWELGTPEGQWVPQSEPLHPINKISSTEPCTGDRSVFCQMEVLD RYCSIPGYHRLCCVSCIKKASGPNPGPDPGPTSLPPFSTPGSPLPGPQDPADAAEPPGKP TGSEDHQHGRATQLPGALDTSSPGTQHPFAPETPIPGASWSISPTTPGGLPWGWTQTPTP VPEDKGQPGEDLRHPGTSLPAASPVT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
