Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850873
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3864nts
Open reading frame: 1 to 3864
ATGCCCCGTAAAGGCACCCAGCCCTCCACTGCCCGGCGCAGAGAGGAAGGGCCGCCGCCG
CCGTCCCCTGACGGCGCCAGCAGCGACGCGGAGCCTGAGCCGCCGTCCGGCCGCACGGAG
AGCCCAGCCACCGCCGCAGAGACTGCAAGTGAGGAACTTGATAATAGAAGTTTAGAAGAG
ATTTTGAACAGCATTCCTCCTCCCCCGCCTCCAGCAATGACCAATGAAGCTGGAGCTCCT
CGGCTTATGATAACTCATATTGTAAACCAGAACTTCAAATCCTATGCTGGGGAGAAAATT
CTGGGACCTTTCCATAAGCGCTTTTCCTGTATTATCGGGCCAAATGGCAGTGGCAAATCC
AATGTTATTGATTCTATGCTTTTTGTGTTTGGCTATCGAGCACAAAAAATAAGATCTAAA
AAACTCTCAGTATTAATACATAATTCTGATGAACACAAGGACATTCAGAGTTGTACAGTA
GAAGTTCATTTTCAAAAGATAATTGATAAGGAAGGGGATGATTATGAAGTCATTCCTAAC
AGTAATTTCTATGTATCCAGAACGGCCTGCAGAGATAATACTTCTGTCTATCACATAAGT
GGAAAGAAAAAGACATTTAAGGATGTTGGAAATCTTCTTCGAAGCCATGGAATTGACTTG
GACCATAATAGATTTTTAATTTTACAGGGTGAAGTTGAACAAATTGCTATGATGAAACCA
AAAGGCCAGACTGAACACGATGAGGGTATGCTTGAATATTTAGAAGATATAATTGGTTGT
GGACGGCTAAATGAACCTATTAAAGTCTTGTGTCGGAGAGTTGAAATATTAAATGAACAC
AGAGGAGAGAAGTTAAACAGGGTAAAGATGGTGGAAAAGGAAAAGGATGCCTTAGAAGGA
GAGAAAAACATAGCTATCGAATTTCTTACCTTGGAAAATGAAATATTTAGAAAAAAGAAT
CATGTTTGTCAATATTATATTTATGAGTTGCAGAAACGAATTGCTGAAATGGAAACTCAA
AAGGAAAAAATTCATGAAGATACCAAAGAAATTAATGAGAAGAGCAATATACTATCAAAT
GAAATGAAAGCTAAGAATAAAGATGTAAAAGATACAGAAAAGAAACTGAATAAAATTACA
AAATTTATTGAGGAGAATAAAGAAAAATTTACACAGCTAGATTTGGAAGATGTTCAAGTT
AGAGAAAAGTTAAAACATGCCACGAGTAAAGCCAAAAAACTGGAGAAACAACTTCAAAAA
GATAAAGAAAAGGTTGAAGAATTTAAAAGTATACCTGCCAAGAGTAACAATATCATTAAT
GAAACAACAACCAGAAACAATGCCCTCGAGAAGGAAAAAGAGAAAGAAGAAAAAAAATTA
AAGGAAGTTATGGATAGCCTTAAACAGGAAACACAAGGGCTTCAGAAAGAAAAAGAAAGT
CGAGAGAAAGAACTTATGGGTTTCAGCAAATCGGTAAATGAAGCACGTTCAAAGATGGAT
GTAGCCCAGTCAGAACTTGATATCTATCTCAGTCGTCATAATACTGCAGTGTCTCAATTA
ACTAAGGCTAAGGAAGCTCTAATTGCAGCTTCTGAGACTCTCAAAGAAAGGAAAGCTGCA
ATCAGAGATATAGAAGGAAAACTCCCTCAAACTGAACAAGAATTAAAGGAGAAAGAAAAA
GAACTTCAAAAACTTACACAAGAAGAAACAAACTTTAAAAGTTTGGTTCATGATCTCTTT
CAAAAAGTTGAAGAAGCAAAGAGCTCATTAGCAATGAATCGAAGTAGGGGGAAAGTCCTT
GATGCAATAATTCAAGAAAAAAAATCTGGCAGGATTCCAGGAATATATGGAAGATTGGGG
GACTTAGGAGCCATTGATGAAAAATACGACGTGGCTATATCATCCTGTTGTCATGCACTG
GACTACATTGTTGTTGATTCTATTGATATAGCCCAAGAATGTGTAAACTTCCTTAAAAGA
CAAAATATTGGAGTTGCAACCTTTATAGGTTTAGATAAGATGGCTGTATGGGCGAAAAAG
ATGACCGAAATTCAAACTCCTGAAAATACTCCTCGTTTATTTGATTTAGTAAAAGTAAAA
GATGAGAAAATTCGCCAAGCTTTTTATTTTGCTTTACGAGATACCTTAGTAGCTGACAAC
TTGGATCAAGCCACAAGAGTAGCATATCAAAAAGATAGAAGATGGAGAGTGGTAACTTTA
CAGGGACAAATCATAGAACAGTCAGGTACAATGACTGGTGGTGGAAGCAAAGTAATGAAA
GGAAGAATGGGTTCCTCACTTGTTATTGAAATCTCTGAAGAAGAGGTAAACAAAATGGAA
TCACAGTTGCAAAACGACTCTAAAAAAGCAATGCAAATCCAAGAACAGAAAGTACAACTT
GAAGAAAGAGTAGTTAAGTTACGGCATAGTGAACGAGAAATGAGGAACACACTAGAAAAA
TTTACTGCAAGCATCCAGCGTTTAATAGAGCAAGAAGAATATTTGAATGTCCAAGTTAAG
GAACTTGAAGCTAATGTACTTGCTACAGCCCCTGACAAAAAAAAGCAGAAATTGCTAGAA
GAAAACGTTAGTGCTTTCAAAACAGAATATGATGCTGTGGCTGAGAAAGCTGGTAAAGTA
GAAGCTGAGGTTAAACGCTTACACAATACCATCGTAGAAATCAATAATCATAAACTCAAG
GCCCAACAAGACAAACTTGATAAAATAAATAAGCAATTAGATGAATGTGCTTCTGCTATT
ACTAAAGCCCAAGTAGCAATCAAGACTGCTGACAGAAACCTTCAAAAGGCACAAGACTCT
GTCTTGCGTACAGAGAAAGAAATAAAAGATACTGAGAAAGAGGTGGATGACCTAACAGCA
GAGCTGAAAAGTCTTGAGGACAAAGCAGCAGAGGTCGTAAAGAATACAAATGCTGCAGAG
GAATCCTTACCAGAGATCCAGAAAGAACATCGCAATCTGCTTCAAGAATTAAAAGTTATT
CAAGAAAATGAACATGCTCTTCAAAAAGATGCACTTAGTATTAAGTTGAAACTTGAACAA
ATAGATGGTCACATTGCTGAACATAATTCTAAAATAAAATATTGGCACAAAGAGATTTCA
AAAATATCACTGCATCCTATAGAAGATAATCCTATTGAAGAGATTTCGGTTCTAAGCCCA
GAGGATCTTGAAGCGATCAAGAATCCAGATTCTATAACAAATCAAATTGCACTTTTGGAA
GCCCGGTGTCATGAAATGAAACCAAACCTCGGTGCCATCGCAGAGTATAAAAAGAAGGAA
GAATTGTATTTGCAACGGGTAGCAGAATTGGACAAAATTACTTATGAAAGAGACAGTTTT
AGACAGGCATATGAAGATCTTCGGAAACAAAGGCTTAATGAATTTATGGCAGGTTTTTAT
ATAATAACAAATAAATTAAAGGAAAATTACCAAATGCTTACTTTGGGAGGGGACGCCGAA
CTCGAGCTTGTAGACAGCTTGGATCCTTTCTCTGAAGGAATCATGTTCAGTGTTCGACCA
CCTAAGAAAAGTTGGAAAAAGATCTTCAACCTTTCGGGAGGAGAGAAAACACTTAGTTCA
TTGGCTTTAGTATTTGCTCTTCACCACTACAAGCCCACTCCCCTTTACTTCATGGATGAG
ATTGATGCAGCCCTTGATTTTAAAAATGTGTCCATTGTTGCATTTTATATATATGAACAA
ACAAAAAATGCACAGTTCATAATAATTTCTCTTCGAAATAATATGTTTGAGATTTCGGAT
AGACTTATTGGAATTTACAAGACATACAACATAACAAAAAGTGTTGCTGTAAATCCAAAA
GAAATTGCATCTAAGGGACTTTGT
Protein sequence predicted based on nucleotide sequence
MPRKGTQPSTARRREEGPPPPSPDGASSDAEPEPPSGRTESPATAAETASEELDNRSLEE ILNSIPPPPPPAMTNEAGAPRLMITHIVNQNFKSYAGEKILGPFHKRFSCIIGPNGSGKS NVIDSMLFVFGYRAQKIRSKKLSVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPN SNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKP KGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEG EKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSN EMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQK DKEKVEEFKSIPAKSNNIINETTTRNNALEKEKEKEEKKLKEVMDSLKQETQGLQKEKES REKELMGFSKSVNEARSKMDVAQSELDIYLSRHNTAVSQLTKAKEALIAASETLKERKAA IRDIEGKLPQTEQELKEKEKELQKLTQEETNFKSLVHDLFQKVEEAKSSLAMNRSRGKVL DAIIQEKKSGRIPGIYGRLGDLGAIDEKYDVAISSCCHALDYIVVDSIDIAQECVNFLKR QNIGVATFIGLDKMAVWAKKMTEIQTPENTPRLFDLVKVKDEKIRQAFYFALRDTLVADN LDQATRVAYQKDRRWRVVTLQGQIIEQSGTMTGGGSKVMKGRMGSSLVIEISEEEVNKME SQLQNDSKKAMQIQEQKVQLEERVVKLRHSEREMRNTLEKFTASIQRLIEQEEYLNVQVK ELEANVLATAPDKKKQKLLEENVSAFKTEYDAVAEKAGKVEAEVKRLHNTIVEINNHKLK AQQDKLDKINKQLDECASAITKAQVAIKTADRNLQKAQDSVLRTEKEIKDTEKEVDDLTA ELKSLEDKAAEVVKNTNAAEESLPEIQKEHRNLLQELKVIQENEHALQKDALSIKLKLEQ IDGHIAEHNSKIKYWHKEISKISLHPIEDNPIEEISVLSPEDLEAIKNPDSITNQIALLE ARCHEMKPNLGAIAEYKKKEELYLQRVAELDKITYERDSFRQAYEDLRKQRLNEFMAGFY IITNKLKENYQMLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSWKKIFNLSGGEKTLSS LALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISD RLIGIYKTYNITKSVAVNPKEIASKGLC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
