Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850909
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5118nts
Open reading frame: 1 to 5118
ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTG
GTGAAAGAGCATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATA
CATCAGTGCTCCCATCCTAATGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATC
TTTACTGATATTTTTCACTACCTGGAGGTGTTGTTTCGCATTATTAAACCCAGGAAAGTG
TTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCAAAAATGAACCAGCAGCGTGGGAGG
CGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCAATAGAGAAGGGAGAA
ACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAATTTATG
GCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCA
TGGCAAGGAGTTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAA
ATCATGGAATTTATCAGATCCGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACAC
TGTCTTTATGGTTTAGATGCTGACTTGATTATGCTTGGATTAACAAGTCATGAGGCACAT
TTTTCTCTCTTAAGAGAAGAAGTTCGATTTGGTGGCAAAAAAACACAACGGGTATGTGCT
CCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTAATGAGAGAGTATATTGACTAT
GAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAAAGGATAATAGAT
GATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCATTTA
CATATTAATCATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAA
CTTGGGGGTTATATTAATGAAAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTT
GTGAAACTATCAGATTTTGATCGGGAGCACTTCAGTGAAGTTTTTGTGGACCTAAAATGG
TTTGAAAGCAAAGTTGGTAACAAGTACCTCAATGAAGCAGCAGGTGTCGCAGCAGAAGAA
GCCAGGAACTACAAGGAAAAGAAAAAGTTAAAGGGCCAGGAAAATTCTCTGTGTTGGACT
GCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGATAATTTAGAAGATGAGACT
GAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATATTACATGACG
AAGATGGGGGTTGACGTAGTATCTGATGACTTTCTGGCTGATCAAGCTGCATGTTATGTT
CAGGCAATACAGTGGATTTTGCACTATTACTATCATGGAGTTCAGTCCTGGAGCTGGTAT
TATCCTTATCATTATGCACCTTTCCTGTCTGATATACACAACATCAGTACACTCAAAATC
CATTTTGAACTAGGAAAACCTTTTAAGCCATTTGAACAGCTTCTTGCTGTACTTCCAGCA
GCCAGCAAAAATTTACTTCCTGCATGCTACCAGCATTTGATGACCAATGAAGACTCACCA
ATTATAGAATATTACCCACCTGATTTTAAAACTGACCTAAATGGGAAACAACAGGAATGG
GAAGCTGTGGTGTTAATCCCTTTTATTGATGAGAAGCGATTATTGGAAGCCATGGAGACA
TGTAACCACTCCCTCAAAAAGGAAGAGAGGAAAAGAAACCAACATAGTGAGTGCCTAATG
TGCTGGTATGATAGAGACACAGAGTTTATCTATCCTTCTCCATGGCCAGAAAAGTTCCCT
GCCATAGAACGATGTTGTACAAGGTATAAAATAATATCCTTAGATGCTTGGCGTGTAGAC
ATAAACAAAAACAAAATAACCAGAATTGACCAGAAAGCATTATATTTCTGTGGATTTCCT
ACTCTGAAACACATCAGACACAAATTTTTTTTGAAGAAAAGTGGTGTTCAAGTATTCCAG
CAAAGCAGTCGTGGAGAAAACATGATGTTGGAAATCTTAGTGGATGCAGAATCAGATGAA
CTTACCGTAGAAAATGTAGCTTCATCAGTGCTTGGAAAATCTGTCTTTGTTAATTGGCCT
CACCTTGAGGAAGCTAGAGTCGTGGCTGTATCAGATGGAGAAACTAAGTTTTACTTGGAA
GAACCTCCAGGAACACAGAAGCTTTATTCAGGAAGAACTGCCCCACCATCTAAAGTGGTT
CATCTTGGAGATAAAGAACAATCTAACTGGGCAAAAGAAGTACAAGGAATTTCAGAACAC
TACCTGAGAAGAAAAGGAATAATAATAAATGAAACATCTGCAGTTGTGTATGCTCAGTTA
CTCACAGGTCGTAAATATCAAATAAATCAAAATGGTGAAGTTCGTCTAGAGAAACAGTGG
TCAAAACAAGTTGTTCCTTTTGTTTATCAAACTATTGTCAAGGACATCCGAGCTTTCGAC
TCCCGTTTCTCCAATATCAAAACATTGGATGATTTGTTTCCTCTGAGAAGTATGGTCTTT
ATGCTGGGAACTCCCTATTATGGCTGCACTGGAGAAGTTCAGGATTCAGGTGATGTGATT
ACAGAAGGTAGGATTCGTGTGATTTTCAGCATTCCATGTGAACCCAATCTTGATGCTTTA
ATACAGAACCAGCATAAATATTCTATAAAGTACAACCCAGGATATGTGTTGGCCAGTCGC
CTTGGAGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAAGTATTTTTATTGGAAGAGGA
TCTAGGAGAAACCCTCATGGAGACCATAAAGCAAATGTGGGTTTAAATCTCAAATTCAAC
AAGAAAAATGAGGAGGTACCTGGATATACTAAGAAAGTTGGAAGTGAATGGATGTATTCA
TCTGCAGCAGAACAACTTCTGGCAGAGTACTTAGAGAGAGCTCCAGAACTATTTAGTTAT
ATAGCCAAAAATAGCCAAGAGGATGTGTTCTATGAAGATGACATTTGGCCTGGAGAAAAT
GAGAATGGTGCTGAAAAAGTTCAAGAAATTATTACTTGGCTAAAAGGACATCCTGTCAGT
ACTTTATCTCGTTCTTCTTGTGATTTACAAATTCTGGATGCAGCTATTGTTGAGAAAATT
GAGGAAGAAGTCGAAAAGTGCAAGCAAAGAAAGAATAATAAGAAGGTGCGAGTAACAGTG
AAACCCCATTTGCTATACAGACCTTTAGAACAGCAACATGGAGTCATTCCTGATCGGGAT
GCAGAATTTTGTCTTTTTGACCGTGTTGTAAATGTGAGAGAAAACTTCTCAGTTCCAGTT
GGCCTTCGAGGCACCATCATAGGAATAAAAGGAGCTAATAGAGAAGCCGATGTACTATTT
GAAGTATTATTTGATGAAGAATTTCCTGGAGGGTTAACAATAAGATGCTCACCTGGTAGA
GGTTATCGACTGCCAACAAGTGCCTTGGTGAACCTTTCTCATGGGAGTCGCTCTGAAACT
GGAAATCAGAAGTTGACAGCCATCGTAAAACCACAACCAGCTGTACATCAACATAGCTCA
AGTTCATCAGTTTCCTCTGGGCATTTGGGAGCCCTCAACCATTCCCCTCAATCACTTTTT
GTTCCTACTCAAGTACCTACTAAAGATGATGATGAATTCTGCAACATTTGGCAGTCCTTA
CAGGGATCTGGAAAGATGCAATACTTTCAGCCAACTATACAAGAGAAGGGTGCAGTTCTA
CCTCAAGAAATAAGCCAAGTAAATCAACATCATAAATCTGGCTTTAATGACAACAGTGTT
AAATATCAGCAAAGAAAACATGACCCTCACAGAAAATTTAAAGAAGAGTGTAAGAGTCCT
AAAGCTGAGTGTTGGTCCCAAAAAATGTCCAATAAGCAGCCTAACTCTGGAATTGAGAAC
TTTTTAGCATCTTTGAATATCTCCAAAGAAAATGAAGTACAGTCATCTCATCATGGGGAG
CCTCCAAGTGAAGAGCATTTGTCACCACAGTCATTTGCCATGGGAACACGGATGCTTAAA
GAAATTCTAAAAATTGATGGCTCTAACACTGTGGACCATAAGAATGAAATCAAACAGATT
GCTAATGAAATCCCTGTTTCCTCTAACAGAAGAGATGAATATGGATTACCCTCTCAGCCT
AAACAAAATAAGAAATTAGCATCTTATATGAACAAGCCTCACAGTGCTAATGAGTACCAT
AATGTTCAGTCTATGGACAATATGTGTTGGCCTGCCCCCAGCCAGATCCCTCCTGTATCC
ACACCAGTAACTGAACTTTCTCGAATTTGTTCCCTTGTTGGAATGCCACAACCTGATTTC
TCCTTTCTTAGGATGCCACAGACAATGACCGTTTGCCAAGTAAAATTATCTAATGGCTTA
CTGGTACATGGGCCACAGTGCCACTCTGAAAATGAAGCCAAAGAGAAAGCTGCACTTTTT
GCTTTACAACAGTTGGGCTCCTTAGGCATGAATTTCCCTTTGCCTTCACAAGTATTTGCA
AATTATCCTTCAGCTGTACCACCTGGAACCATTCCTCCAGCCTTTCCCCCACCTACTGGC
TGGGATCACTATGGAAGCAACTATGCATTGGGGGCAGCTAATATAATGCCTTCGTCGTCT
CATCTCTTTGGCTCAATGCCATGGGGACCATCGGTGCCAGTTCCTGGGAAGCCCTTCCAT
CATACTTTATATTCTGGGACCATGCCCATGGCTGGGGGAATACCAGGGGGTGTGCACAAT
CAGTTTATACCTCTGCAGGTTACTAAAAAAAGGGTTGCAAACAAAAAGAACTTTGAGAAT
AAGGAAGCCCAGAGTTCTCAAGCCACTCCAGTTCAGACTAGCCAGCCAGATTCTTCCAAC
ATTGTCAAAGTAAGTCCACGGGAGAGCTCATCAGCTTCTTTGAAGTCCTCTCCGATTGCT
CAACCTGCATCTTCTTTTCAAGTTGAAACTGCCTCTCAAGGCCATAGTATATCTCACCAT
AAGTCAACACCAATCTCTTCTTCAAGAAGAAAATCAAGAAAACTGGCTGTTAATTTTGGT
GTTTCTAAACCTTCTGAG
Protein sequence predicted based on nucleotide sequence
MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKI FTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGE TLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHK IMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCA PEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIIDDWILMGFLVGNDFIPHLPHL HINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREHFSEVFVDLKW FESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDET EDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWY YPYHYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSP IIEYYPPDFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLM CWYDRDTEFIYPSPWPEKFPAIERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFP TLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWP HLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEH YLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFD SRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDAL IQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFN KKNEEVPGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGEN ENGAEKVQEIITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTV KPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLF EVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSS SSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVL PQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIEN FLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQI ANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVS TPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALF ALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTGWDHYGSNYALGAANIMPSSS HLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFEN KEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHH KSTPISSSRRKSRKLAVNFGVSKPSE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
