Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00851000
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2658nts
Open reading frame: 1 to 2658
ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGG
CACATAAGACCCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTG
TGCCCAGCTTATGCCACCTGCACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAA
GGCTTCCTGTCCAGCAATGGGCAAAATCACTTCAAGGATCCAGGAGTGCGATGCAAAGAT
ATTGATGAATGTTCTCAAAGCCCCCAGCCCTGTGGTCCTAACTCATCCTGCAAAAACCTG
TCAGGGAGGTACAAGTGCAGCTGTTTAGATGGTTTCTCTTCTCCCACTGGAAATGACTGG
GTCCCAGGAAAGCCGGGCAATTTCTCCTGTACTGATATCAATGAGTGCCTCACCAGCAGC
GTCTGCCCTGAGCATTCTGACTGTGTCAACTCCATGGGAAGCTACAGTTGCAGCTGTCAA
GTTGGATTCATCTCTAGAAACTCCACCTGTGAAGACGTGGATGAATGTGCAGATCCAAGA
GCTTGCCCAGAGCATGCAACTTGTAATAACACTGTTGGAAACTACTCTTGTTTCTGCAAC
CCAGGATTTGAATCCAGCAGTGGCCACTTGAGTTTCCAGGGTCTCAAAGCATCGTGTGAA
GATATTGATGAATGCACTGAAATGTGCCCCATCAATTCAACATGCACCAACACTCCTGGG
AGCTACTTTTGCACCTGCCACCCTGGCTTTGCACCAAGCAATGGACAGTTGAATTTCACA
GACCAAGGAGTGGAATGTAGAGATATTGATGAGTGCCGCCAAGATCCATCAACCTGTGGT
CCTAATTCTATCTGCACCAATGCCCTGGGCTCCTACAGCTGTGGCTGCATTGCAGGCTTT
CATCCCAATCCAGAAGGCTCCCAGAAAGATGGCAACTTCAGCTGCCAAAGGGTTCTCTTC
AAATGTAAGGAAGATGTGATACCCGATAATAAGCAGATCCAGCAATGCCAAGAGGGAACC
GCAGTGAAACCTGCATATGTCTCCTTTTGTGCACAAATAAATAACATCTTCAGCGTTCTG
GACAAAGTGTGTGAAAATAAAACGACCGTAGTTTCTCTGAAGAATACAACTGAGAGCTTT
GTCCCTGTGCTTAAACAAATATCCACGTGGACTAAATTCACCAAGGAAGAGACGTCCTCC
CTGGCCACAGTCTTCCTGGAGAGTGTGGAAAGCATGACACTGGCATCTTTTTGGAAACCC
TCAGCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGACATTGAGAGCAAAGTTATC
AACAAAGAATGCAGTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGGGATAAGATG
AAGATCGGGTGTTCCACAATTGAGGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTT
GTCTCCTTTGTGGGCATGGAATCGGTTTTAAATGAGCGCTTCTTCAAAGACCACCAGGCT
CCCTTGACCACCTCTGAGATCAAGCTGAAGATGAATTCTCGAGTCGTTGGGGGCATAATG
ACTGGAGAGAAGAAAGACGGCTTCTCAGATCCAATCATCTACACTCTGGAGAACATTCAG
CCAAAGCAGAAGTTTGAGAGGCCCATCTGTGTTTCCTGGAGCACTGATGTGAAGGGTGGA
AGATGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGAGACATATACCATCTGCAGC
TGTAATCAGATGGCAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACGATGGACTTT
TCCTTGTACATCATTAGCCATGTAGGCATTATCATCTCCTTGGTGTGCCTCGTCTTGGCC
ATCGCCACCTTTCTGCTGTGTCGCTCCATCCGAAATCACAACACCTACCTCCACCTGCAC
CTCTGCGTGTGTCTCCTCTTGGCGAAGACTCTCTTCCTCGCCGGTATACACAAGACTGAC
AACAAGATGGGCTGCGCCATCATCGCGGGCTTCCTGCACTACCTTTTCCTTGCCTGCTTC
TTCTGGATGCTGGTGGAGGCTGTGATACTGTTCTTGATGGTCAGAAACCTGAAGGTGGTG
AATTACTTCAGCTCTCGCAACATCAAGATGCTGCACATCTGTGCCTTTGGTTATGGGCTG
CCGATGCTGGTGGTGGTGATCTCTGCCAGTGTGCAGCCACAGGGCTATGGAATGCATAAT
CGCTGCTGGCTGAATACAGAGACAGGGTTCATCTGGAGTTTCTTGGGGCCAGTTTGCACA
GTTATAGTGATCAACTCCCTTCTCCTGACCTGGACCTTGTGGATCCTGAGGCAGAGGCTT
TCCAGTGTTAATGCCGAAGTCTCAACGCTAAAAGACACCAGGTTACTGACCTTCAAGGCC
TTTGCCCAGCTCTTCATCCTGGGCTGCTCCTGGGTGCTGGGCATTTTTCAGATTGGACCT
GTGGCAGGTGTCATGGCTTACCTGTTCACCATCATCAACAGCCTGCAGGGGGCCTTCATC
TTCCTCATCCACTGTCTGCTCAACGGCCAGGTACGAGAAGAATACAAGAGGTGGATCACT
GGGAAGACGAAGCCCAGCTCCCAGTCCCAGACCTCAAGGATCTTGCTGTCCTCCATGCCA
TCCGCTTCCAAGACGGGT
Protein sequence predicted based on nucleotide sequence
MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQ GFLSSNGQNHFKDPGVRCKDIDECSQSPQPCGPNSSCKNLSGRYKCSCLDGFSSPTGNDW VPGKPGNFSCTDINECLTSSVCPEHSDCVNSMGSYSCSCQVGFISRNSTCEDVDECADPR ACPEHATCNNTVGNYSCFCNPGFESSSGHLSFQGLKASCEDIDECTEMCPINSTCTNTPG SYFCTCHPGFAPSNGQLNFTDQGVECRDIDECRQDPSTCGPNSICTNALGSYSCGCIAGF HPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCAQINNIFSVL DKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSSLATVFLESVESMTLASFWKP SANITPAVRTEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAF VSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQ PKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGELTMDF SLYIISHVGIIISLVCLVLAIATFLLCRSIRNHNTYLHLHLCVCLLLAKTLFLAGIHKTD NKMGCAIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICAFGYGL PMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTWTLWILRQRL SSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFI FLIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLSSMPSASKTG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
