Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00858798
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3066nts
Open reading frame: 1 to 3066
ATGGAAGGGGCGCTGACTGCCCGGGACAAGGTCGGGGTTCAGGATTTTGTGCTATTGGAC
GCGTACACCAGCGAATCTGCCTTTGTCGACAACCTCCGCAAGCGTTTCAGCGAGAACCTC
ATATACACATATATTGGCACCCTCCTTGTGTCTGTGAATCCATACCAGGAGCTCGGAATC
TACACTGTGAGCCAGATGGAACTTTATCAAGGGGTCAATTTCTTTGAACTGCCACCACAT
GTCTACGCTATAGCCGACAACGCTTACCGAATGATGTGTGCTGAACTAAATAACCATTTC
ATCCTCATTTCTGGAGAGAGTGGGGCAGGGAAAACAGAGGCCTCCAAGAAAATTCTCGAG
TATTTTGCAGTGACCTGCCCAATGACCCAGTCCCTACAAATAGCCCGTGACAGACTGCTG
TTCTCCAACCCAGTGCTCGAGGCTTTTGGAAATGCCAGAACGCTTCGGAATGACAACTCT
AGCAGATTTGGGAAATACATGGATATACAATTTGATTTTCAGGGCATTCCCGTAGGTGGG
CATATCATCAGTTACTTGATAGAGAAGTCCCGAGTTGTCTACCAAAACGAAGGCGAGCGG
AATTTCCACATCTTCTACCAGCTGCTGGCAGGCGGCGAAGAGGAGCGCCTGTCTTACCTG
GGACTCGAGCGAGATCCCCAGCTGTACAAATACCTCTCACAGGGTCATTGTGCCAAAGAG
TCATCCATTAGTGACAAGAACGACTGGAAAACTGTTTCCAACGCCTTCTCTGTCATTGAT
TTTACTGAAGCTGACCTCGAGAATCTCTTTGGAATTATTGCCAGTGTCTTACACCTGGGG
AACATTGGTTTTGAAGAAGACGACCAAGGCTGTGCCACTATCCCCGACACCCATGAGATC
AAGTGGATAGCCAAGCTCCTGGGGGTCCACCCATCAGTCCTTCTCGAAGCTCTCACCCAC
AGAAAAATTGAAGCCAAAACTGAGGAGGTGATCTGCCCGTTGACACTAGAACTGTCTGTG
TACGCTAGAGATGCAATGGCAAAGGCTGTTTATGGACGAACGTTTACTTGGCTGGTCAAC
AAAATTAATTCCTCCTTAGTGAACAAGGATTTCACCAGGAAAACCGTAATTGGATTACTG
GACATCTACGGGTTTGAAGTCTTTGACAAGAATGGCTTTGAGCAGTTCTGTATAAATTAC
TGCAACGAGAAACTCCAGCAACTGTTAATTGAGAGGACTCTAAAAGCAGAACAGGCAGAA
TATGAAATGGAAGGCATAGAGTGGGAGCCAATTAAGTATTTCAACAACAAGATCATCTGT
GACCTGGTCGAGGAGAGACATAAAGGAATCATATCTATTCTGGATGAAGAATGCATTCGG
CCTGGTCCTGCTACAGACTTGAGTTTCCTGGAGAAATTGGAAGAGAAAGTGGGCAAACAT
GCACACTTCGAAACCCGTAAGCTCGCTGGTCCAAAGGGCCGAAAGAGGATTGGCTGGATG
GAGTTCCGACTCCTCCACTATGCAGGAGAGGTCACATACTGCACCAAGGGATTCCTGGAA
AAAAACAATGATCTTCTTTACAGACATCTGAAAGAAGTGCTGTGCAAGTCCAAGAACATT
ATCCTGAGGGAATGCTTCCTGCTGGCCGAGTTAGAAAACCGGAGGAGGCCCCCAACAGTG
GGGACTCAGTTTAAAAACAGTCTGAGCAGCCTTCTAGAAACCCTCATCTCTAAGGAGCCC
TCCTACATCCGTTGCATCAAGCCCAACGACAGGAAAGAACCCAGCAAATTTGATGACTTC
CTCATAAGGCATCAGATTAAATACCTGGGGCTGATGGAGCACCTCCGGGTGAGACGGGCT
GGTTTTGCATACCGAAGGAAATACGAGCATTTCTTGCAAAGGTACAAGTCTTTATGCCCA
GACACCTGGCCGCACTGGCACGGGCCTCCAGCAGAGGGCGTGGAACGGCTGATCAAGTAC
ATCGGCTACAAACCCGAGGAATACAAGTTAGGCAAGACCAAAATATTCATTCGTTTCCCC
AGAACTCTCTTTGCTACCGAAGATGCCTTTGAATTTAGTAAACATCAACTAGTGGCAAGA
ATCCAAGCCACTTACAAACGCTGCCTAGGAAGGAGAGAATACGTGAAAAAAAGACAAGCA
GCCATCAAACTGGAAGCCCACTGGCGTGGGGCCCTGGCTCGGAAGGCAATCCAAAGGAGA
AAATGGGCCGTGCGGATTATCAGAAAGTTCATTAAAGGATTCATCAGTCGCAACAAACCC
CTCTGTCCTGACAACGAGGAATTTATCGTGTTTGTGCGGAAGAATTACATCTTGAATCTC
CGGTATCACCTTCCAAAGACTGTCCTGGACAAGAGCTGGCTCAGGCCTCCTGGCATCTTG
GAAAATGCATCAGATCTGCTCAGGAAAATGTGCGTGAGAAACCTGGTGCAGAAGTACTGC
CGCGGGATCACAGCTGAGCGGAAAGCAATGATGCAGCAAAAGGTAGTTACAAGTGAAATC
TTCAGGGGAAGGAAAGACGGCTACACAGAAAGTTTAAATCAACCCTTTGTCAACAGTCGG
ATAGATGAAGGAGACATTAATCCGAAAGTGCTTCAGCTAATTAGCCATGAGAAAATCCAG
TATGGTGTCCCGGTCATTAAATATGACAGAAAAGGCTTCAAAGCACGGCAGCGGCAACTC
ATTCTTACTCAGAAAGCAGCTTACGTGGTGGAACTTGCCAAAATCAAGCAGAAAATAGAG
TACTCAGCTCTCAAAGGTGTCTCCACTAGCAATCTGAGTGATGGAATCTTAGTCATTCAT
GTTTCACCAGAGGACAGCAAGCAAAAGGGGGATGCCGTTCTGCAGTGTGGACACGTGTTT
GAAGCAGTTACTAAACTCGTCATGCTGGTTAAGAAGGAGAACATTGTCAATGTTGTTCAA
GGCAGCTTGCAGTTTTTTATTAGCCCGGGAAAAGAAGGCACAATAGTTTTCGACACCGGA
CTGGAAGAACAAGTCTATAAAAATAAAAATGGACAATTGACAGTGGTGTCAGTCCGGAGG
AAGTCC
Protein sequence predicted based on nucleotide sequence
MEGALTARDKVGVQDFVLLDAYTSESAFVDNLRKRFSENLIYTYIGTLLVSVNPYQELGI YTVSQMELYQGVNFFELPPHVYAIADNAYRMMCAELNNHFILISGESGAGKTEASKKILE YFAVTCPMTQSLQIARDRLLFSNPVLEAFGNARTLRNDNSSRFGKYMDIQFDFQGIPVGG HIISYLIEKSRVVYQNEGERNFHIFYQLLAGGEEERLSYLGLERDPQLYKYLSQGHCAKE SSISDKNDWKTVSNAFSVIDFTEADLENLFGIIASVLHLGNIGFEEDDQGCATIPDTHEI KWIAKLLGVHPSVLLEALTHRKIEAKTEEVICPLTLELSVYARDAMAKAVYGRTFTWLVN KINSSLVNKDFTRKTVIGLLDIYGFEVFDKNGFEQFCINYCNEKLQQLLIERTLKAEQAE YEMEGIEWEPIKYFNNKIICDLVEERHKGIISILDEECIRPGPATDLSFLEKLEEKVGKH AHFETRKLAGPKGRKRIGWMEFRLLHYAGEVTYCTKGFLEKNNDLLYRHLKEVLCKSKNI ILRECFLLAELENRRRPPTVGTQFKNSLSSLLETLISKEPSYIRCIKPNDRKEPSKFDDF LIRHQIKYLGLMEHLRVRRAGFAYRRKYEHFLQRYKSLCPDTWPHWHGPPAEGVERLIKY IGYKPEEYKLGKTKIFIRFPRTLFATEDAFEFSKHQLVARIQATYKRCLGRREYVKKRQA AIKLEAHWRGALARKAIQRRKWAVRIIRKFIKGFISRNKPLCPDNEEFIVFVRKNYILNL RYHLPKTVLDKSWLRPPGILENASDLLRKMCVRNLVQKYCRGITAERKAMMQQKVVTSEI FRGRKDGYTESLNQPFVNSRIDEGDINPKVLQLISHEKIQYGVPVIKYDRKGFKARQRQL ILTQKAAYVVELAKIKQKIEYSALKGVSTSNLSDGILVIHVSPEDSKQKGDAVLQCGHVF EAVTKLVMLVKKENIVNVVQGSLQFFISPGKEGTIVFDTGLEEQVYKNKNGQLTVVSVRR KS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
