Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00858985
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3975nts
Open reading frame: 1 to 3975
ATGTCGCGGAGGAAGCAAGCGAAGCCTCAACATTTCCAATCCGACCCCGAAGTGGCCTCG
CTCCCCCGGCGAGATGGAGACACAGAAAAGGGTCAACCGAGTCGCCCTACTAAGAGCAAG
GATGCCCACGTCTGTGGCCGGTGCTGTGCCGAGTTCTTTGAATTATCAGATCTTCTGCTC
CACAAGAAGAACTGTACTAAAAATCAATTAGTTTTAATCGTAAATGAAAATCCAGCCTCC
CCACCCGAAACCTTCTCCCCCAGCCCCCCTCCTGATAATCCTGATGAACAAATGAATGAC
ACAGTTAACAAAACAGATCAAGTGGACTGCAGCGACCTTTCAGAACACAACGGACTTGAC
AGGGAAGAGTCCATGGAGGTGGAGGCCCCGGTTGCTAACAAAAGCGGCAGCGGCACTTCC
AGCGGCAGCCACAGCAGTACCGCCCCAAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGGC
GGCGGCGGCAGCTCCTCCACAGGTACCTCAGCGATCACAACCTCTCTACCTCAACTCGGG
GACCTGACAACACTGGGCAACTTCTCCGTAATCAACAGCAACGTCATCATCGAGAACCTC
CAGAGCACCAAGGTGGCGGTGGCCCAGTTCTCCCAGGAAGCGAGGTGCGGCGGGGCCTCT
GGGGGCAAGCTGGCCGTCCCAGCCCTCATGGAACAACTCCTAGCTCTGCAGCAGCAGCAG
ATCCACCAGCTGCAATTGATCGAACAGATTCGTCACCAAATATTGCTGTTGGCTTCTCAG
AATGCAGACTTGCCAACATCTTCTAGTCCTTCTCAAGGTACTTTACGAACATCTGCCAAC
CCCTTGTCCACGCTAAGTTCCCATTTATCTCAGCAGCTGGCAGCAGCAGCTGGATTGGCA
CAGAGCCTCGCCAGCCAATCTGCCAGCATTAGTGGTGTGAAACAGCTACCCCCAATCCAG
CTACCTCAGAGCAGTTCTGGCAACACCATCATTCCATCCAACAGCGGCTCTTCTCCCAAT
ATGAACATATTGGCAGCGGCAGTTACCACCCCGTCCTCTGAAAAAGTGGCTTCAAGTGCT
GGGGCCTCCCATGTCAGCAACCCAGCGGTCTCATCATCGTCCTCACCAGCTTTTGCAATA
AGCAGTTTATTAAGTCCTGCGTCTAATCCACTTCTACCTCAGCAAGCCTCCGCTAACTCG
GTTTTCCCCAGCCCTTTGCCCAACATCGGAACAACTGCAGAGGATTTAAACTCCTTGTCT
GCCTTGGCCCAGCAAAGAAAAAGCAAGCCACCAAATGTCACTGCCTTTGAAGCGAAAAGT
ACTTCCGATGAGGCATTCTTCAAACACAAGTGCAGGTTCTGCGCGAAGGTCTTTGGGAGT
GACAGTGCCTTGCAGATCCACTTGCGTTCCCATACCGGAGAGAGGCCATTCAAGTGCAAC
ATCTGCGGGAACAGGTTCTCCACCAAGGGGAATCTGAAAGTCCACTTTCAGCGCCACAAA
GAGAAATACCCTCATATCCAGATGAACCCCTATCCTGTGCCTGAGCATTTGGACAATATC
CCCACGAGTACTGGCATCCCATATGGCATGTCCATCCCTCCAGAGAAGCCAGTCACCAGC
TGGCTAGACACCAAACCAGTCCTGCCTACTCTGACCACTTCAGTCGGCCTGCCGTTGCCC
CCAACCCTCCCAAGCCTCATACCCTTCATCAAGACGGAAGAGCCAGCCCCCATCCCCATC
AGCCATTCTGCCACCAGCCCCCCAGGCTCAGTCAAAAGTGACTCCGGGGGCCCTGAGTCA
GCCACAAGAAACCTAGGTGGGCTCCCAGAGGAAGCCGAAGGGTCCACTCTGCCACCCTCT
GGTGGCAAAAGCGAAGAGAGTGGCATGGTCACCAACTCAGTCCCGACGGCGAGCAGTAGC
GTCCTGAGCTCCCCAGCGGCAGACTGCGGCCCCGCGGGCAGTGCCACCACCTTCACCAAC
CCTTTGTTGCCGCTCATGTCCGAGCAGTTCAAGGCCAAGTTTCCTTTTGGGGGACTCCTG
GACTCAGCTCAGGCATCAGAGACGTCCAAGCTTCAGCAACTGGTAGAAAACATTGACAAG
AAGGCCACTGACCCCAATGAGTGCATCATCTGCCACCGGGTTCTCAGCTGCCAGAGCGCC
TTGAAAATGCACTACAGGACACACACTGGGGAGAGGCCCTTTAAGTGTAAGATCTGTGGC
CGGGCTTTCACCACGAAAGGGAATCTTAAAACCCACTACAGTGTCCATCGTGCTATGCCC
CCGCTCAGAGTCCAGCATTCCTGCCCCATCTGCCAGAAGAAGTTCACGAACGCTGTGGTC
CTGCAGCAGCACATCCGAATGCATATGGGAGGCCAGATCCCCAACACCCCAGTCCCCGAC
AGCTACTCTGAGTCCATGGAGTCTGACACAGGTTCCTTTGATGAGAAAAATTTTGATGAC
CTAGACAACTTCTCTGATGAAAACATGGAAGACTGTCCTGAGGGCAGCATCCCTGATACA
CCTAAGTCTGCAGACGCCTCCCAAGACAGCTTATCCTCTTCGCCTTTGCCCCTCGAGATG
TCGAGCATCGCTGCTTTGGAAAATCAGATGAAGATGATCAATGCTGGCCTGGCAGAGCAG
CTACAGGCCAGCCTGAAGTCAGTGGAGAATGGGTCCATCGAGGGGGATGTCCTGACCAAT
GATTCATCCTCAGTGGGTGGTGACATGGAAAGCCAAAGTGCTGGCAGCCCAGCCATCTCA
GAGTCTACCTCTTCCATGCAGGCTCTGTCCCCGTCCAACAGCACGCAGGAGTTCCACAAG
TCACCCAGCATTGAGGAGAAACCACAGAGAGCGGTCCCAAGCGAGTTTGCCAATGGTTTG
TCTCCCACCCCAGTGAATGGTGGGGCTTTGGATTTGACATCTAGTCACGCAGAGAAAATC
ATCAAAGAAGATTCTTTGGGGATCCTCTTCCCTTTTAGAGACCGGGGTAAATTTAAAAAC
ACTGCTTGTGACATTTGTGGCAAAACATTTGCTTGTCAGAGTGCCTTGGACATTCACTAT
AGAAGTCATACCAAAGAGAGACCATTTATTTGCACAGTTTGCAATCGTGGCTTTTCCACA
AAGGGTAATTTGAAGCAGCACATGTTGACACATCAGATGCGAGATCTGCCATCCCAGCTC
TTTGAGCCCAGTTCCAACCTTGGCCCCAATCAGAACTCAGCGGTGATTCCCGCCAACTCG
TTGTCATCTCTCATCAAGACAGAGGTCAACGGCTTCGTGCATGTTTCTCCTCAGGACAGT
AAGGACACCCCCACCAGTCACGTCCCGTCTGGGCCTCTGTCTTCCTCTGCCACATCCCCA
GTTCTGCTCCCTGCTCTGCCCAGGAGAACTCCCAAGCAGCACTACTGCAACACATGTGGC
AAAACCTTCTCCTCATCGAGTGCCCTGCAGATTCACGAGAGAACTCACACTGGAGAGAAA
CCCTTTGCTTGCACTATTTGTGGAAGAGCTTTCACGACTAAAGGCAATCTTAAGGTACAC
ATGGGCACTCACATGTGGAATAGCACCCCTGCACGACGGGGTCGGCGGCTCTCTGTGGAT
GGCCCCATGACATTTCTAGGAGGCAATCCCGTCAAGTTCCCAGAAATGTTCCAGAAGGAT
TTGGCGGCAAGATCAGGAAGTGGGGATCCTTCCAGCTTCTGGAATCAGTATGCAGCAGCG
CTCTCCAACGGGCTGGCGATGAAGGCCAACGAGATCTCCGTCATTCAGAACGGTGGCATC
CCTCCAATTCCTGGAAGCCTCGGCAGTGGGAACAGCTCACCTGTTAGTGGGCTGACGGGA
AACCTGGAGAGGCTCCAGAACTCAGAGCCCAATGCTCCCCTGGCCGGCCTGGAGAAAATG
GCAAGCAGTGAGAACGGAACCAACTTCCGCTTCACCCGCTTCGTGGAGGACAGCAAGGAG
ATCGTCACGAGTTTG
Protein sequence predicted based on nucleotide sequence
MSRRKQAKPQHFQSDPEVASLPRRDGDTEKGQPSRPTKSKDAHVCGRCCAEFFELSDLLL HKKNCTKNQLVLIVNENPASPPETFSPSPPPDNPDEQMNDTVNKTDQVDCSDLSEHNGLD REESMEVEAPVANKSGSGTSSGSHSSTAPSSSSSSSSSSGGGGSSSTGTSAITTSLPQLG DLTTLGNFSVINSNVIIENLQSTKVAVAQFSQEARCGGASGGKLAVPALMEQLLALQQQQ IHQLQLIEQIRHQILLLASQNADLPTSSSPSQGTLRTSANPLSTLSSHLSQQLAAAAGLA QSLASQSASISGVKQLPPIQLPQSSSGNTIIPSNSGSSPNMNILAAAVTTPSSEKVASSA GASHVSNPAVSSSSSPAFAISSLLSPASNPLLPQQASANSVFPSPLPNIGTTAEDLNSLS ALAQQRKSKPPNVTAFEAKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCN ICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTS WLDTKPVLPTLTTSVGLPLPPTLPSLIPFIKTEEPAPIPISHSATSPPGSVKSDSGGPES ATRNLGGLPEEAEGSTLPPSGGKSEESGMVTNSVPTASSSVLSSPAADCGPAGSATTFTN PLLPLMSEQFKAKFPFGGLLDSAQASETSKLQQLVENIDKKATDPNECIICHRVLSCQSA LKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVV LQQHIRMHMGGQIPNTPVPDSYSESMESDTGSFDEKNFDDLDNFSDENMEDCPEGSIPDT PKSADASQDSLSSSPLPLEMSSIAALENQMKMINAGLAEQLQASLKSVENGSIEGDVLTN DSSSVGGDMESQSAGSPAISESTSSMQALSPSNSTQEFHKSPSIEEKPQRAVPSEFANGL SPTPVNGGALDLTSSHAEKIIKEDSLGILFPFRDRGKFKNTACDICGKTFACQSALDIHY RSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSNLGPNQNSAVIPANS LSSLIKTEVNGFVHVSPQDSKDTPTSHVPSGPLSSSATSPVLLPALPRRTPKQHYCNTCG KTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVD GPMTFLGGNPVKFPEMFQKDLAARSGSGDPSSFWNQYAAALSNGLAMKANEISVIQNGGI PPIPGSLGSGNSSPVSGLTGNLERLQNSEPNAPLAGLEKMASSENGTNFRFTRFVEDSKE IVTSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
