Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859161
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4590nts
Open reading frame: 1 to 4590
ATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGGGTTAGTGCTGGCGATCCTG
AACAAGGTGGCACCGCAGGCGTGCCCGGCGCAGTGCTCTTGCTCGGGCAGCACAGTGGAC
TGTCACGGGCTGGCGCTGCGCAGCGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTG
GATTTAAATGGAAATAACATCACAAGAATTACGAAGACAGATTTTGCTGGTCTTAGACAT
CTAAGAGTTCTTCAGCTTATGGAGAATAAGATTAGCACCATTGAAAGAGGAGCATTCCAG
GATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCACCTTCAGCTGTTTCCTGAG
TTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCTCAGTGAAAACCAAATTCAG
GCAATCCCAAGGAAAGCTTTCCGTGGGGCAGTTGACATAAAAAATTTGCAACTGGATTAC
AACCAGATCAGCTGTATTGAAGATGGGGCATTCAGGGCTCTCCGGGACCTGGAAGTGCTC
ACTCTCAACAATAACAACATTACTAGACTTTCTGTGGCAAGTTTCAACCATATGCCTAAA
CTTAGGACTTTTCGACTGCATTCAAACAACCTGTATTGTGACTGCCACCTGGCCTGGCTC
TCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCTGTACACTCAGTGTATGGGCCCCTCC
CACCTGAGAGGCCATAATGTAGCCGAGGTTCAAAAACGAGAATTTGTCTGCAGTGGTCAC
CAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTGCCCTGCCGCCTGTACCTGTAGC
AACAATATCGTAGACTGTCGTGGGAAAGGTCTCACTGAGATCCCCACAAATCTTCCAGAG
ACCATCACAGAAATACGTTTGGAACAGAACACAATCAAAGTCATCCCTCCTGGAGCTTTC
TCACCATATAAAAAGCTTAGACGAATTGACCTGAGCAATAATCAGATCTCTGAACTTGCA
CCAGATGCTTTCCAAGGACTACGCTCTCTGAATTCACTTGTCCTCTATGGAAATAAAATC
ACAGAACTCCCCAAAAGTTTATTTGAAGGACTGTTTTCCTTACAGCTCCTATTATTGAAT
GCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCAGGATCTCCACAACTTGAACCTT
CTCTCCCTATATGACAACAAGCTTCAGACCATCGCCAAGGGGACCTTTTCACCTCTTCGG
GCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTATTTGTGACTGCCATCTCAAGTGG
CTAGCGGATTATCTCCATACCAACCCGATTGAGACCAGTGGTGCCCGTTGCACCAGCCCC
CGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGCT
AAAGAACAGTATTTCATTCCAGGTACAGAAGATTATCGATCAAAATTAAGTGGAGACTGC
TTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGAAGGAACCACAGTAGATTGCTCT
AATCAAAAGCTCAACAAAATCCCGGAGCACATTCCCCAGTACACTGCAGAGTTGCGTCTC
AATAATAATGAATTTACCGTGTTGGAAGCCACAGGAATCTTTAAGAAACTTCCTCAATTA
CGTAAAATAAACTTTAGCAACAATAAGATCACAGATATTGAGGAGGGAGCATTTGAAGGA
GCATCTGGTGTAAATGAAATACTTCTTACGAGTAATCGTTTGGAAAATGTGCAGCATAAG
ATGTTCAAGGGATTGGAAAGCCTCAAAACTTTGATGTTGAGAAGCAATCGAATAACCTGT
GTGGGGAATGACAGTTTCATAGGACTCAGTTCTGTGCGTTTGCTTTCTTTGTATGATAAT
CAAATTACTACAGTTGCACCAGGGGCATTTGATACTCTCCATTCTTTATCTACTCTAAAC
CTCTTGGCCAATCCTTTTAACTGTAACTGCTACCTGGCTTGGTTGGGAGAGTGGCTGAGA
AAGAAGAGAATTGTCACGGGAAATCCTAGATGTCAAAAACCATACTTCCTGAAAGAAATA
CCCATCCAGGATGTGGCCATTCAGGACTTCACTTGTGATGACGGAAATGATGACAATAGT
TGCTCCCCACTTTCTCGCTGTCCTACTGAATGTACTTGCTTGGATACAGTCGTCCGATGT
AGCAACAAGGGTTTGAAGGTCTTGCCGAAAGGTATTCCAAGAGATGTCACAGAGTTGTAT
CTGGATGGAAACCAATTTACACTGGTTCCCAAGGAACTCTCCAACTACAAACATTTAACA
CTTATAGACTTAAGTAACAACAGAATAAGCACGCTTTCTAATCAGAGCTTCAGCAACATG
ACCCAGCTCCTCACCTTAATTCTTAGTTACAACCGTCTGAGATGTATTCCTCCTCGCACC
TTTGATGGATTAAAGTCTCTTCGATTACTTTCTCTACATGGAAATGACATTTCTGTTGTG
CCTGAAGGTGCTTTCAATGATCTTTCTGCATTATCACATCTAGCAATTGGAGCCAACCCT
CTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAG
CCTGGAATTGCTCGTTGTGCTGGTCCTGGAGAAATGGCAGATAAACTTTTACTCACAACT
CCCTCCAAAAAATTTACCTGTCAAGGTCCTGTGGATGTCAATATTCTAGCTAAGTGTAAC
CCCTGCCTATCAAATCCGTGTAAAAATGATGGCACATGTAATAGTGATCCAGTTGACTTT
TACCGATGCACCTGTCCATATGGTTTCAAGGGGCAGGACTGTGATGTCCCAATTCATGCC
TGCATCAGTAACCCATGTAAACATGGAGGAACTTGCCACTTAAAGGAAGGAGAAGAAGAT
GGATTCTGGTGTATTTGTGCTGATGGATTTGAAGGAGAAAATTGTGAAGTCAACGTTGAT
GATTGTGAAGATAATGACTGTGAAAATAATTCTACATGTGTCGATGGCATTAATAACTAC
ACATGCCTTTGCCCACCTGAGTATACAGGTGAGTTGTGTGAGGAGAAGCTGGACTTCTGT
GCCCAGGACCTGAACCCCTGCCAGCACGATTCAAAGTGCATCCTAACTCCAAAGGGATTC
AAATGTGACTGCACACCAGGGTACGTAGGTGAACACTGCGACATCGATTTTGACGACTGC
CAAGACAACAAGTGTAAAAACGGAGCCCACTGCACAGATGCAGTGAACGGCTATACGTGC
ATATGCCCCGAAGGTTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCT
CGTACCAGCCCCTGTGATAATTTTGATTGTCAGAATGGAGCTCAGTGTATCGTCAGAATA
AATGAGCCAATATGTCAGTGTTTGCCTGGCTATCAGGGAGAAAAGTGTGAAAAATTGGTT
AGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGATTCCTTCAGCCAAGGTTCGGCCT
CAGACGAACATAACACTTCAGATTGCCACAGATGAAGACAGCGGAATCCTCCTGTATAAG
GGTGACAAAGACCATATCGCGGTAGAACTCTATCGGGGGCGTGTTCGTGCCAGCTATGAC
ACCGGCTCTCATCCAGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTC
CACATTGTGGAACTACTTGCCTTGGATCAGAGTCTCTCTTTGTCCGTGGATGGTGGGAAC
CCCAAAATCATCACTAACTTGTCAAAGCAGTCCACTCTGAATTTTGACTCTCCACTCTAT
GTAGGAGGCATGCCAGGGAAGAGTAACGTGGCATCTCTGCGCCAGGCCCCTGGGCAGAAC
GGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACATCAACAGTGAGCTGCAGGACTTC
CAGAAGGTGCCGATGCAAACAGGCATTTTGCCTGGCTGTGAGCCATGCCACAAGAAGGTG
TGTGCCCATGGCACATGCCAGCCCAGCAGCCAGGCAGGCTTCACCTGCGAGTGCCAGGAA
GGATGGATGGGGCCCCTCTGTGACCAACGGACCAATGACCCTTGCCTTGGAAATAAATGC
GTACATGGCACCTGCTTGCCCATCAATGCGTTCTCCTACAGCTGTAAGTGCTTGGAGGGC
CATGGAGGTGTCCTCTGTGATGAAGAGGAGGATCTGTTTAACCCATGCCAGGCGATCAAG
TGCAAGCATGGGAAGTGCAGGCTTTCAGGTCTGGGGCAGCCCTACTGTGAATGCAGCAGT
GGATACACGGGGGACAGCTGTGATCGAGAAATCTCTTGTCGAGGGGAAAGGATAAGAGAT
TATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAACAACCAAGAAGGTGTCCCGATTA
GAGTGCAGAGGTGGGTGTGCAGGAGGGCAGTGCTGTGGACCGCTGAGGAGCAAGCGGCGG
AAATACTCTTTCGAATGCACTGACGGCTCCTCCTTTGTGGACGAGGTTGAGAAAGTGGTG
AAGTGCGGCTGTACGAGGTGTGTGTCCTTG
Protein sequence predicted based on nucleotide sequence
MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERL DLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPE LLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVL TLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPS HLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPE TITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKI TELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLR AIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSA KEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRL NNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHK MFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLN LLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNS CSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLT LIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVV PEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTT PSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHA CISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNY TCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDC QDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRI NEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYK GDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGN PKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDF QKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKC VHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSS GYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRR KYSFECTDGSSFVDEVEKVVKCGCTRCVSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
