Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859255
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4668nts
Open reading frame: 1 to 4668
ATGGGCTGCAAGGGAGACGCGAGCGGTGCGTGTGCCGCGGGTGCGCTGCCGGTGACAGGA
GTTTGCTATAAAATGGGAGTTCTGGTTGTACTCACTGTTCTGTGGCTGTTCTCCTCAGTA
AAGGCCGACTCAAAAGCCATTACAACCTCTCTTACAACAAAATGGTTTTCCACTCCATTG
TTGTTAGAAGCCAGTGAGTTTTTAGCAGAAGACAGTCAAGAGAAATTTTGGAATTTTGTA
GAAGCCAGTCAAAATATTGGATCATCAGATCATGACGGTACCGATTATTCCTACTATCAT
GCAATATTGGAGGCTGCATTTCAGTTTCTGTCACCCCTCCAGCAGAATTTGTTTAAATTT
TGTCTGTCCCTTCGTTCTTACTCAGCTACAATCCAAGCCTTCCAGCAGATAGCAGCTGAT
GAACCTCCACCAGAAGGATGTAATTCGTTTTTTTCAGTGCATGGAAAGAAGACTTGTGAA
TCTGATACCCTTGAGGCTCTTCTACTGACAGCCTCTGAAAGACCCAAACCTTTATTGTTC
AAAGGAGATCACAGATATCCCTCGTCTAATCCTGAAAGCCCTGTGGTGATTTTCTACTCT
GAGATTGGCTCTGAGGAATTTTCCAATTTTCACCGCCAGCTTATATCAAAAAGCAATGCA
GGCAAAATCAATTATGTATTCAGACATTATATATTTAATCCCAGGAAGGAGCCTGTTTAC
CTCTCTGGCTATGGCGTGGAATTGGCCATTAAGAGCACTGAGTACAAGGCCAAGGATGAT
ACTCAGGTGAAAGGAACTGAGGTAAACACCACAGTGATTGGTGAAAATGATCCTATTGAT
GAGGTTCAGGGGTTCCTCTTTGGAAAATTAAGAGATCTGCACCCCGACCTGGAGGGACAG
TTGAAAGAACTCAGAAAGCATCTTGTAGAGAGCACCAATGAAATGGCACCTTTAAAGGTT
TGGCAGTTGCAAGATCTCAGTTTCCAGACTGCTGCTCGAATCTTGGCTTCTCCTGTTGAG
TTGGCTTTGGTTGTCATGAAGGATCTTAGTCAGAATTTTCCTACCAAAGCCAGAGCAATA
ACAAAAACAGCTGTGAGCTCAGAACTTAGAACCGAAGTGGAAGAGAATCAGAAGTATTTC
AAGGGAACTTTAGGATTACAACCTGGAGATTCAGCCCTCTTCATCAATGGACTTCACATG
GATTTAGATACACAGGATATATTCAGTCTGTTTGATGTGTTGAGGAATGAAGCTCGGGTA
ATGGAGGGTCTGCATAGATTGGGAATAGAAGGCCTTTCTCTGCATAATGTTTTGAAGCTG
AACATCCAGCCCTCTGAGGCAGACTATGCCGTAGACATCCGGAGTCCTGCTATTTCATGG
GTCAACAACCTGGAGGTTGATAGCAGATATAATTCGTGGCCTTCTAGTTTACAAGAGTTG
CTTCGACCCACCTTTCCTGGTGTTATTCGGCAGATCAGGAAAAACTTACATAATATGGTT
TTCATAGTTGATCCTGCACATGAGACCACAGCAGAGTTGATGAACACAGCTGAGATGTTC
CTTAGTAATCATATACCACTAAGAATTGGTTTTATCTTTGTGGTTAATGACTCTGAAGAT
GTTGATGGGATGCAAGATGCTGGAGTGGCTGTTCTTAGAGCATATAATTATGTTGCCCAA
GAAGTGGATGATTATCATGCCTTCCAGACTCTGACACATATCTATAACAAGGTGAGGACT
GGAGAAAAAGTGAAAGTTGAACATGTGGTCAGTGTCCTGGAGAAGAAATATCCGTATGTA
GAAGTGAATAGCATTTTGGGGATTGATTCTGCTTATGATCGGAATCGGAAGGAAGCAAGA
GGCTACTATGAGCAGACTGGAGTTGGACCTCTGCCCGTTGTGCTGTTCAATGGAATGCCC
TTTGAAAGGGAACAGCTAGACCCTGATGAGTTAGAAACCATCACAATGCATAAAATCCTG
GAGACCACCACCTTCTTCCAAAGAGCGGTGTACTTGGGTGAACTGCCCCATGATCAAGAT
GTGGTAGAGTATATCATGAATCAGCCAAATGTTGTTCCACGAATCAATTCTAGGATTTTG
ACAGCTGAACGAGACTACCTGGATTTAACAGCGAGTAATAACTTCTTTGTGGATGATTAT
GCTAGATTTACTATCTTGGATTCCCAAGGCAAGACTGCTGCTGTAGCCAATAGTATGAAC
TATCTGACAAAGAAAGGAATGTCCTCCAAGGAAATCTATGATGATTCTTTTATTAGGCCA
GTAACTTTTTGGATTGTTGGGGATTTTGATAGCCCTTCTGGACGGCAGTTACTGTATGAT
GCCATCAAACATCAGAAATCCAGTAACAATGTTAGAATAAGCATGATCAATAATCCTGCC
AAAGAGATAAGCTATGAGAACACTCAGATCTCCAGAGCAATCTGGGCAGCTCTCCAAACT
CAGACTTCCAACGCTGCTAAGAACTTCATCACCAAAATGGCCAAGGAGGGGGCTGCAGAG
GCCCTGGCTGCAGGAGCTGACATTGCGGAGTTCTCTGTTGGGGGAATGGATTTCAGTCTT
TTTAAAGAGGTCTTTGAGTCTTCCAAAATGGATTTCATTTTGTCTCATGCCGTGTACTGC
AGGGATGTTCTGAAGCTGAAGAAGGGACAGAGGGCAGTGATCAGCAATGGAAGGATCATT
GGGCCACTGGAGGATAGTGAGCTCTTTAATCAAGACGATTTCCACCTCCTCGAAAATATC
ATCTTAAAAACCTCAGGACAGAAAATAAAATCTCATATTCAACAGCTTCGGGTAGAAGAA
GATGTGGCAAGCGACTTGGTAATGAAGGTGGATGCTCTTCTGTCAGCGCAACCAAAAGGA
GATCCAAGAATCGAGTACCAGTTTTTTGAAGACAGACACAGTGCAATCAAACTGAGGCCG
AAGGAAGGGGAGACATACTTTGATGTTGTGGCTGTCGTTGACCCTGTCACCAGAGAAGCA
CAGAGACTTGCTCCTTTGCTCTTGGTTTTGGCTCAGCTGATAAACATGAATCTGAGAGTA
TTTATGAACTGCCAATCCAAACTTTCTGACATGCCTTTAAAAAGCTTTTACCGTTATGTC
TTAGAACCAGAGATTTCTTTCACTTCAGACAATAGTTTTGCTAAGGGTCCAATCGCAAAA
TTTTTGGATATGCCTCAGTCTCCACTGTTCACTCTGAATTTGAACACACCTGAGAGCTGG
ATGGTAGAATCTGTCAGAACACCATATGATCTTGATAATATTTATTTAGAAGAGGTGGAC
AGTGTAGTGGCTGCTGAGTATGAGCTGGAATACCTGTTACTGGAAGGTCATTGCTACGAC
ATCACCACAGGCCAGCCTCCACGGGGACTACAGTTTACCTTAGGAACTTCAGCCAACCCG
GTCATTGTGGACACCATTGTTATGGCCAATCTGGGCTACTTTCAGCTGAAAGCCAACCCA
GGAGCTTGGATCCTCAGACTTAGGAAGGGACGCTCTGAAGATATTTATAGAATTTACAGC
CACGATGGCACTGATTCTCCCCCTGATGCTGATGAGGTGGTTATCGTCCTCAACAACTTC
AAAAGCAAAATTATTAAAGTGAAGGTTCAGAAGAAGGCAGATATGGTGAACGAAGACTTG
CTGAGTGATGGAACGAGTGAGAATGAATCTGGATTTTGGGATTCCTTCAAATGGGGCTTT
ACAGGACAGAAGACTGAGGAAGTGAAGCAAGATAAAGATGACATAATTAATATTTTCTCC
GTTGCATCTGGTCATCTCTACGAAAGATTTCTTCGCATAATGATGCTATCCGTGCTGAAG
AATACCAAGACTCCTGTGAAATTCTGGTTCTTGAAGAATTACTTGTCCCCCACATTTAAG
GAGTTTATACCTTACATGGCAAATGAATACAATTTCCAGTATGAGCTTGTTCAGTACAAA
TGGCCCCGGTGGCTTCATCAACAAACTGAAAAACAGCGTATCATCTGGGGTTACAAGATC
CTCTTCCTGGATGTACTTTTCCCACTAGTTGTTGACAAGTTCCTGTTTGTGGATGCTGAT
CAGATTGTACGAACAGATCTGAAAGAGTTAAGAGATTTCAATTTGGATGGTGCTCCTTAT
GGTTACACTCCTTTCTGTGACAGCCGAAGAGAAATGGACGGCTACAGGTTCTGGAAGTCA
GGGTACTGGGCCAGTCATTTAGCCGGGCGAAAGTATCATATCAGTGCACTATATGTTGTG
GATCTGAAGAAGTTTAGGAAAATAGCTGCTGGTGACAGACTCAGGGGACAGTACCAAGGT
CTGAGTCAGGACCCTAACAGCCTTTCAAATCTTGATCAAGATCTGCCCAATAACATGATT
CATCAGGTGCCAATTAAATCCCTCCCTCAAGAATGGCTTTGGTGTGAAACGTGGTGTGAT
GACGCCTCTAAGAAAAGGGCAAAAACCATTGATTTGTGTAATAATCCGATGACCAAAGAG
CCGAAACTGGAAGCAGCTGTGCGGATTGTCCCGGAGTGGCAGGACTACGACCAAGAGATC
AAACAGCTACAGATCCGCTTTCAGAAGGAGAAAGAAACGGGAGCACTGTACAAAGAGAAG
ACAAAAGAACCAAGCCGAGAAGGTCCTCAGAAACGTGAAGAATTATTG
Protein sequence predicted based on nucleotide sequence
MGCKGDASGACAAGALPVTGVCYKMGVLVVLTVLWLFSSVKADSKAITTSLTTKWFSTPL LLEASEFLAEDSQEKFWNFVEASQNIGSSDHDGTDYSYYHAILEAAFQFLSPLQQNLFKF CLSLRSYSATIQAFQQIAADEPPPEGCNSFFSVHGKKTCESDTLEALLLTASERPKPLLF KGDHRYPSSNPESPVVIFYSEIGSEEFSNFHRQLISKSNAGKINYVFRHYIFNPRKEPVY LSGYGVELAIKSTEYKAKDDTQVKGTEVNTTVIGENDPIDEVQGFLFGKLRDLHPDLEGQ LKELRKHLVESTNEMAPLKVWQLQDLSFQTAARILASPVELALVVMKDLSQNFPTKARAI TKTAVSSELRTEVEENQKYFKGTLGLQPGDSALFINGLHMDLDTQDIFSLFDVLRNEARV MEGLHRLGIEGLSLHNVLKLNIQPSEADYAVDIRSPAISWVNNLEVDSRYNSWPSSLQEL LRPTFPGVIRQIRKNLHNMVFIVDPAHETTAELMNTAEMFLSNHIPLRIGFIFVVNDSED VDGMQDAGVAVLRAYNYVAQEVDDYHAFQTLTHIYNKVRTGEKVKVEHVVSVLEKKYPYV EVNSILGIDSAYDRNRKEARGYYEQTGVGPLPVVLFNGMPFEREQLDPDELETITMHKIL ETTTFFQRAVYLGELPHDQDVVEYIMNQPNVVPRINSRILTAERDYLDLTASNNFFVDDY ARFTILDSQGKTAAVANSMNYLTKKGMSSKEIYDDSFIRPVTFWIVGDFDSPSGRQLLYD AIKHQKSSNNVRISMINNPAKEISYENTQISRAIWAALQTQTSNAAKNFITKMAKEGAAE ALAAGADIAEFSVGGMDFSLFKEVFESSKMDFILSHAVYCRDVLKLKKGQRAVISNGRII GPLEDSELFNQDDFHLLENIILKTSGQKIKSHIQQLRVEEDVASDLVMKVDALLSAQPKG DPRIEYQFFEDRHSAIKLRPKEGETYFDVVAVVDPVTREAQRLAPLLLVLAQLINMNLRV FMNCQSKLSDMPLKSFYRYVLEPEISFTSDNSFAKGPIAKFLDMPQSPLFTLNLNTPESW MVESVRTPYDLDNIYLEEVDSVVAAEYELEYLLLEGHCYDITTGQPPRGLQFTLGTSANP VIVDTIVMANLGYFQLKANPGAWILRLRKGRSEDIYRIYSHDGTDSPPDADEVVIVLNNF KSKIIKVKVQKKADMVNEDLLSDGTSENESGFWDSFKWGFTGQKTEEVKQDKDDIINIFS VASGHLYERFLRIMMLSVLKNTKTPVKFWFLKNYLSPTFKEFIPYMANEYNFQYELVQYK WPRWLHQQTEKQRIIWGYKILFLDVLFPLVVDKFLFVDADQIVRTDLKELRDFNLDGAPY GYTPFCDSRREMDGYRFWKSGYWASHLAGRKYHISALYVVDLKKFRKIAAGDRLRGQYQG LSQDPNSLSNLDQDLPNNMIHQVPIKSLPQEWLWCETWCDDASKKRAKTIDLCNNPMTKE PKLEAAVRIVPEWQDYDQEIKQLQIRFQKEKETGALYKEKTKEPSREGPQKREELL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
