Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859286
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6396nts
Open reading frame: 1 to 6396
ATGGCCGAGCGCCGCGCCTTCGCCCAGAAGATCAGCAGAACGGTGGCAGCCGAAGTTAGG
AAGCAGATCTCCGGACAATATAGTGGTTCTCCCCAACTGCTCAAAAACCTTAATATTGTT
GGCAATATATCCCATCACACCACAGTGCCCCTTACCGAAGCAGTAGATCCAGTGGATTTG
GAAGATTACCTCATTACTCATCCTTTGGCTGTGGATTCTGGGCCTTTACGGGATTTGATT
GAATTTCCTCCAGATGATATTGAAGTTGTTTATAGTCCTCGGGACTGCAGAACTCTTGTT
TCAGCTGTACCTGAAGAAAGTGAAATGGATCCACATGTTAGAGACTGTATAAGAAGTTAT
ACAGAAGACTGGGCAATTGTCATCAGAAAATATCATAAATTGGGAACAGGATTTAATCCC
AATACATTAGATAAACAGAAAGAAAGGCAAAAAGGTTTGCCAAAACAAGTTTTTGAATCT
GATGAAGCTCCAGATGGCAACAGCTACCAGGATGATCAAGATGACCTTAAAAGACGTTCA
ATGTCAATAGATGATACCCCAAGGGGTAGCTGGGCCTGTAGTATCTTTGACTTGAAAAAT
TCACTTCCTGATGCTTTGCTTCCCAATTTACTTGATCGAACTCCAAATGAAGAAATAGAC
CGTCAGAATGATGACCAAAGGAAATCAAACCGTCACAAAGAACTTTTTGCTTTGCATCCA
TCACCAGATGAGGAAGAACCAATAGAACGGCTTAGTGTTCCTGATATACCCAAAGAACAT
TTTGGTCAAAGACTTCTTGTAAAATGCTTATCACTCAAGTTTGAAATTGAAATTGAACCC
ATTTTTGCAAGTTTGGCTTTATATGATGTCAAGGAAAAGAAAAAGATTTCAGAAAACTTT
TATTTTGACCTTAATTCTGAGCAGATGAAAGGGTTGTTACGTCCACATGTACCACCTGCT
GCCATTACTACCCTGGCAAGATCAGCAATTTTTTCTATCACTTATCCTTCCCAAGATGTT
TTTCTTGTAATAAAGCTAGAAAAAGTCCTACAGCAAGGAGACATTGGAGAGTGTGCAGAA
CCATATATGATTTTCAAAGAAGCAGATGCCACCAAGAATAAAGAAAAACTGGAGAAACTG
AAGAGTCAAGCAGATCAGTTTTGCCAAAGACTTGGGAAATATCGCATGCCTTTTGCTTGG
ACTGCAATCCATTTAATGAATATTGTTAGCAGTGCTGGGAGTTTGGAAAGAGATTCTACA
GAAGTAGAAATCAGTACTGGAGAACGAAAAGGGTCTTGGTCAGAGAGGAGGAATTCTAGT
ATTGTTGGCAGACGATCACTTGAAAGGACAACAAGTGGAGATGATGCTTGTAACTTGACG
AGCTTTCGACCAGCTACTCTCACAGTGACAAATTTTTTTAAGCAGGAAGGAGACCGCTTA
AGTGATGAAGATCTCTACAAATTCCTTGCTGATATGAGAAGGCCATCTTCTGTCTTACGG
CGACTAAGACCTATTACAGCTCAGCTCAAGATAGACATTTCTCCCGCACCTGAAAATCCC
CATTATTGCCTAACTCCGGAGCTGCTTCAAGTGAAGCTTTACCCTGACAGTAGAGTTAGA
CCTACCAGAGAAATCTTAGAGTTTCCCGCAAGGGATGTTTATGTTCCAAACACTACTTAC
AGAAATCTTCTCTACATATACCCTCAGAGTCTTAATTTTGCCAATCGTCAAGGTTCTGCT
AGAAATATAACAGTGAAAGTCCAGTTTATGTATGGAGAGGATCCAAGCAATGCCATGCCG
GTAATCTTTGGTAAATCTAGCTGTTCAGAATTTTCAAAGGAAGCCTATACAGCCGTAGTA
TATCATAACAGGTCTCCTGATTTTCATGAAGAAATCAAGGTTAAGCTTCCTGCTACTTTA
ACTGACCATCATCACTTGCTTTTTACTTTTTATCATGTTAGTTGTCAACAAAAACAAAAT
ACTCCTCTTGAAACACCAGTTGGATATACATGGATACCAATGCTTCAGAATGGACGGTTG
AAGACTGGCCAGTTTTGCTTGCCAGTCTCATTGGAAAAACCACCACAGGCTTATTCTGTA
CTGTCTCCTGAGGTTCCTCTACCTGGCATGAAATGGGTAGATAATCACAAAGGTGTTTTT
AATGTTGAAGTTGTTGCTGTTTCGTCTATCCATACACAAGATCCTTATCTTGACAAATTT
TTTGCTCTGGTCAATGCTCTGGATGAACACCTGTTCCCAGTCCGAATTGGGGACATGCGA
ATCATGGAAAATAACTTAGAAAATGAATTGAAGAGCAGTATTTCAGCACTGAATTCATCC
CAGCTGGAACCAGTGGTCCGATTTCTTCATCTTCTGCTAGATAAACTGATACTTTTAGTT
ATTAGACCTCCTGTCATTGCTGGCCAAATAGTTAACCTAGGTCAAGCATCTTTTGAAGCC
ATGGCATCAATTATAAATCGACTTCACAAAAACTTGGAAGGAAATCATGACCAGCATGGC
AGAAACAGCCTTCTTGCATCATATATTCATTATGTTTTCCGCCTACCAAATACTTACCCT
AATTCATCATCACCAGGTCCTGGGGGTTTGGGAGGATCAGTGCATTATGCCACAATGGCT
AGATCTGCGGTGAGACCTGCAAGCCTTAATTTAAATCGTTCTCGAAGCCTTAGTAATAGC
AATCCAGATATATCTGGGACTCCCACGTCACCAGATGATGAAGTTCGATCAATCATCGGG
AGTAAGGGTTTAGATCGCTCCAATTCCTGGGTTAACACTGGTGGTCCAAAAGCTGCCCCA
TGGGGATCCAACCCCAGTCCAAGTGCAGAATCAACACAGGCTATGGATCGAAGTTGTAAT
CGTATGTCTTCGCACACAGAGACGTCAAGTTTCTTACAAACATTAACGGGACGCTTACCA
ACTAAAAAGCTTTTTCACGAGGAGCTGGCTTTGCAGTGGGTTGTTTGCAGTGGCAGCGTT
CGGGAATCAGCTTTGCAACAAGCCTGGTTCTTTTTTGAATTAATGGTAAAGAGCATGGTG
CACCATTTATACTTTAATGATAAACTTGAGGCTCCAAGGAAAAGTCGTTTTCCAGAACGT
TTCATGGATGACATTGCAGCTCTTGTCAGCACGATTGCTAGTGATATAGTTTCACGATTT
CAGAAGGACACAGAAATGGTTGAGAGACTCAATACAAGCCTTGCATTCTTTCTCAATGAT
CTGTTGTCTGTTATGGACAGAGGATTTGTTTTTAGCCTTATAAAGTCCTGCTATAAACAG
GTGTCTTCAAAGCTTTACTCATTACCGAATCCCAGTGTTCTGGTGTCCTTGAGGCTGGAT
TTTCTACGAATCATCTGCAGTCATGAGCACTATGTTACATTAAACTTACCCTGCAGCTTA
CTTACTCCACCTGCATCTCCATCACCTTCTGTTTCTTCTGCAACATCTCAGAGTTCTGGA
TTTTCTACGAATGTACAAGACCAAAAGATTGCAAATATGTTTGAATTATCCGTGCCTTTC
CGCCAACAGCATTATTTGGCAGGACTTGTGTTAACAGAGCTGGCTGTCATTTTAGACCCT
GATGCTGAAGGACTGTTTGGATTGCATAAGAAAGTCATCAATATGGTACACAATTTACTC
TCCAGTCACGACTCAGACCCGCGGTACTCTGACCCTCAGATAAAGGCTCGAGTGGCCATG
TTGTATCTACCTCTGATTGGTATTATCATGGAAACTGTACCTCAGCTGTATGATTTTACA
GAAACTCACAATCAACGAGGAAGACCAATTTGTATAGCCACTGATGATTATGAAAGTGAG
AGCGGAAGTATGATAAGCCAGACCGTTGCCATGGCAATCGCAGGGACATCGGTCCCTCAA
CTAACAAGGCCTGGCAGTTTCCTCCTCACGTCAACGAGTGGCAGGCAACACACTACCTTT
TCAGCAGAATCAAGTCGAAGCCTTTTGATCTGTCTACTTTGGGTTCTCAAAAATGCAGAT
GAAACAGTTCTACAGAAGTGGTTTACAGATCTCTCAGTCTTGCAGCTAAACCGGCTATTA
GATCTGCTTTATCTCTGTGTGTCTTGCTTTGAGTATAAAGGGAAAAAAGTGTTTGAACGA
ATGAATAGCTTGACCTTTAAGAAATCAAAAGACATGAGAGCAAAGCTTGAAGAAGCTATT
CTTGGGAGCATAGGTGCCAGGCAAGAAATGGTACGGCGAAGCCGAGGACAGCTCGAGAGA
AGCCCATCTGGAAGTGCCTTTGGAAGTCAAGAAAATTTGAGGTGGAGGAAAGATATGACT
CACTGGCGTCAAAACACAGAGAAGCTTGACAAATCAAGAGCAGAGATTGAACACGAAGCA
CTGATTGATGGAAACCTGGCTACAGAAGCAAACCTAATCATTTTAGATACATTAGAGATT
GTTGTTCAGACCGTTTCTGTAACGGAATCCAAAGAGAGCATTCTTGGTGGAGTGCTAAAA
GTGCTACTACACAGCATGGCCTGTAACCAAAGTGCAGTTTATCTACAACACTGTTTTGCT
ACACAGAGAGCCTTGGTTTCAAAGTTTCCTGAACTCTTATTTGAAGAAGAGACAGAGCAG
TGTGCTGATTTATGCCTCAGGCTTCTCCGACACTGTAGCAGTAGCATCGGTACAATACGG
TCACACGCCAGTGCCTCCCTTTACCTACTAATGAGGCAAAACTTTGAGATTGGGAATAAC
TTTGCCAGGGTTAAAATGCAGGTAACAATGTCACTATCCTCCTTGGTGGGCACATCTCAG
AATTTTAATGAAGAATTCTTAAGACGTTCTCTAAAGACTATATTGACATATGCTGAAGAA
GATCTGGAATTGAGGGAAACAACATTTCCTGATCAGGTCCAGGATCTGGTTTTCAATCTC
CATATGATTCTTTCTGATACTGTGAAAATGAAGGAACACCAGGAGGATCCTGAAATGTTG
ATTGATCTAATGTACAGAATTGCCAAGGGTTACCAGACCTCTCCAGATCTGCGATTGACC
TGGTTGCAGAACATGGCAGGCAAGCACTCAGAACGAAGCAATCATGCTGAAGCTGCACAG
TGTCTAGTCCACTCAGCAGCACTTGTTGCTGAATATTTGAGCATGCTGGAGGACCGGAAA
TATCTTCCTGTGGGATGTGTAACATTTCAGAATATTTCATCTAATGTTTTAGAAGAATCT
GCGGTCTCAGATGATGTGGTATCTCCAGATGAAGAAGGTATCTGCTCTGGAAAATACTTT
ACTGAGTCAGGACTTGTGGGATTACTGGAACAAGCAGCTGCTTCCTTCTCTATGGCTGGC
ATGTATGAAGCAGTTAATGAAGTTTACAAAGTACTTATTCCTATTCATGAAGCTAATCGG
GATGCAAAGAAACTATCCACAATTCATGGTAAACTTCAAGAAGCATTCAGCAAAATTGTT
CATCAGAGTACTGGCTGGGAGCGGATGTTTGGCACCTATTTTCGTGTTGGTTTTTATGGA
ACCAAGTTCGGGGATTTGGATGAACAAGAATTTGTTTACAAGGAGCCTGCAATAACCAAA
CTTGCAGAGATATCTCACAGATTGGAGGGATTTTACGGAGAAAGATTTGGAGAGGATGTG
GTTGAAGTAATCAAAGACTCTAATCCTGTAGACAAGTGTAAATTAGATCCTAACAAGGCA
TATATTCAGATTACCTATGTGGAGCCATACTTTGACACATATGAGATGAAGGACAGAATC
ACCTATTTCGACAAAAATTACAATCTTCGTCGATTCATGTACTGTACACCCTTTACTTTA
GATGGCCGTGCCCATGGGGAACTTCATGAACAATTCAAAAGGAAGACCATTCTGACTACG
TCTCATGCCTTTCCTTATATTAAAACAAGGGTCAATGTCACTCATAAAGAAGAGATCATC
TTAACACCAATTGAAGTTGCTATTGAGGACATGCAGAAAAAGACACAGGAGTTGGCATTT
GCAACACATCAGGATCCCGCAGACCCCAAAATGCTTCAGATGGTACTCCAGGGATCTGTA
GGCACCACAGTGAATCAGGGGCCTTTGGAAGTTGCCCAGGTTTTTCTGTCTGAAATACCT
AGTGACCCAAAGCTCTTCAGACATCATAATAAACTGCGACTCTGCTTTAAAGATTTTACT
AAAAGGTGTGAAGATGCCTTAAGAAAAAATAAGAGCTTAATTGGGCCGGATCAAAAGGAG
TATCAAAGGGAACTGGAGAGAAACTATCATCGCCTTAAAGAGGCCCTACAGCCACTGATC
AACAGAAAGATCCCTCAGTTATACAAGGCAGTATTGCCTGTCACCTGCCACAGAGATTCC
TTCAGTCGAATGAGCCTTCGCAAAATGGATCTCTTG
Protein sequence predicted based on nucleotide sequence
MAERRAFAQKISRTVAAEVRKQISGQYSGSPQLLKNLNIVGNISHHTTVPLTEAVDPVDL EDYLITHPLAVDSGPLRDLIEFPPDDIEVVYSPRDCRTLVSAVPEESEMDPHVRDCIRSY TEDWAIVIRKYHKLGTGFNPNTLDKQKERQKGLPKQVFESDEAPDGNSYQDDQDDLKRRS MSIDDTPRGSWACSIFDLKNSLPDALLPNLLDRTPNEEIDRQNDDQRKSNRHKELFALHP SPDEEEPIERLSVPDIPKEHFGQRLLVKCLSLKFEIEIEPIFASLALYDVKEKKKISENF YFDLNSEQMKGLLRPHVPPAAITTLARSAIFSITYPSQDVFLVIKLEKVLQQGDIGECAE PYMIFKEADATKNKEKLEKLKSQADQFCQRLGKYRMPFAWTAIHLMNIVSSAGSLERDST EVEISTGERKGSWSERRNSSIVGRRSLERTTSGDDACNLTSFRPATLTVTNFFKQEGDRL SDEDLYKFLADMRRPSSVLRRLRPITAQLKIDISPAPENPHYCLTPELLQVKLYPDSRVR PTREILEFPARDVYVPNTTYRNLLYIYPQSLNFANRQGSARNITVKVQFMYGEDPSNAMP VIFGKSSCSEFSKEAYTAVVYHNRSPDFHEEIKVKLPATLTDHHHLLFTFYHVSCQQKQN TPLETPVGYTWIPMLQNGRLKTGQFCLPVSLEKPPQAYSVLSPEVPLPGMKWVDNHKGVF NVEVVAVSSIHTQDPYLDKFFALVNALDEHLFPVRIGDMRIMENNLENELKSSISALNSS QLEPVVRFLHLLLDKLILLVIRPPVIAGQIVNLGQASFEAMASIINRLHKNLEGNHDQHG RNSLLASYIHYVFRLPNTYPNSSSPGPGGLGGSVHYATMARSAVRPASLNLNRSRSLSNS NPDISGTPTSPDDEVRSIIGSKGLDRSNSWVNTGGPKAAPWGSNPSPSAESTQAMDRSCN RMSSHTETSSFLQTLTGRLPTKKLFHEELALQWVVCSGSVRESALQQAWFFFELMVKSMV HHLYFNDKLEAPRKSRFPERFMDDIAALVSTIASDIVSRFQKDTEMVERLNTSLAFFLND LLSVMDRGFVFSLIKSCYKQVSSKLYSLPNPSVLVSLRLDFLRIICSHEHYVTLNLPCSL LTPPASPSPSVSSATSQSSGFSTNVQDQKIANMFELSVPFRQQHYLAGLVLTELAVILDP DAEGLFGLHKKVINMVHNLLSSHDSDPRYSDPQIKARVAMLYLPLIGIIMETVPQLYDFT ETHNQRGRPICIATDDYESESGSMISQTVAMAIAGTSVPQLTRPGSFLLTSTSGRQHTTF SAESSRSLLICLLWVLKNADETVLQKWFTDLSVLQLNRLLDLLYLCVSCFEYKGKKVFER MNSLTFKKSKDMRAKLEEAILGSIGARQEMVRRSRGQLERSPSGSAFGSQENLRWRKDMT HWRQNTEKLDKSRAEIEHEALIDGNLATEANLIILDTLEIVVQTVSVTESKESILGGVLK VLLHSMACNQSAVYLQHCFATQRALVSKFPELLFEEETEQCADLCLRLLRHCSSSIGTIR SHASASLYLLMRQNFEIGNNFARVKMQVTMSLSSLVGTSQNFNEEFLRRSLKTILTYAEE DLELRETTFPDQVQDLVFNLHMILSDTVKMKEHQEDPEMLIDLMYRIAKGYQTSPDLRLT WLQNMAGKHSERSNHAEAAQCLVHSAALVAEYLSMLEDRKYLPVGCVTFQNISSNVLEES AVSDDVVSPDEEGICSGKYFTESGLVGLLEQAAASFSMAGMYEAVNEVYKVLIPIHEANR DAKKLSTIHGKLQEAFSKIVHQSTGWERMFGTYFRVGFYGTKFGDLDEQEFVYKEPAITK LAEISHRLEGFYGERFGEDVVEVIKDSNPVDKCKLDPNKAYIQITYVEPYFDTYEMKDRI TYFDKNYNLRRFMYCTPFTLDGRAHGELHEQFKRKTILTTSHAFPYIKTRVNVTHKEEII LTPIEVAIEDMQKKTQELAFATHQDPADPKMLQMVLQGSVGTTVNQGPLEVAQVFLSEIP SDPKLFRHHNKLRLCFKDFTKRCEDALRKNKSLIGPDQKEYQRELERNYHRLKEALQPLI NRKIPQLYKAVLPVTCHRDSFSRMSLRKMDLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
