Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859336
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6075nts
Open reading frame: 1 to 6075
ATGGCCACAGGAGGAGGTCCCTTTGAAGATGGCATGAATGATCAGGATTTACCAAACTGG
AGTAATGAGAATGTTGATGACAGGCTCAACAATATGGATTGGGGTGCCCAACAGAAGAAA
GCAAATAGATCATCAGAAAAGAATAAGAAAAAGTTTGGTGTAGAAAGTGATAAAAGAGTA
ACCAATGATATTTCTCCGGAGTCGTCACCAGGAGTTGGAAGGCGAAGAACAAAGACTCCA
CATACGTTCCCACACAGTAGATACATGAGTCAGATGTCTGTCCCAGAGCAGGCAGAATTA
GAGAAACTGAAACAGCGGATAAACTTCAGTGATTTAGATCAGAGAAGCATTGGAAGTGAT
TCCCAAGGTAGAGCAACAGCTGCTAACAACAAACGTCAGCTTAGTGAAAACCGAAAGCCC
TTCAACTTTTTGCCTATGCAGATTAATACTAACAAGAGCAAAGATGCATCTACAAGTCCC
CCAAACAGAGAAACGATTGGATCAGCACAGTGTAAAGAGTTGTTTGCTTCTGCTTTAAGT
AATGACCTCTTGCAAAACTGTCAGGTGTCTGAAGAAGATGGGAGGGGAGAACCTGCAATG
GAGAGCAGCCAGATTGTAAGCAGGCTTGTTCAAATTCGCGATTATATTACTAAAGCTAGT
TCCATGCGGGAAGATCTTGTAGAGAAAAATGAGAGATCTGCTAATGTTGAGCGCCTTACT
CATCTAATAGATCACCTTAAAGAACAAGAGAAGTCATATATGAAATTTCTTAAAAAAATC
CTTGCCAGAGATCCTCAGCAGGAGCCTATGGAAGAGATAGAAAATTTGAAGAAACAACAT
GATTTATTAAAAAGAATGTTACAACAGCAGGAGCAACTAAGAGCTCTACAGGGACGGCAG
GCTGCACTTCTAGCTCTGCAACATAAAGCAGAGCAAGCTATTGCAGTGATGGATGATTCT
GTTGTTGCAGAAACTGCAGGTAGCTTATCTGGCGTCAGTATCACATCTGAACTAAATGAA
GAATTGAATGACTTAATTCAGCGTTTTCATAATCAGCTTCGTGATTCTCAGCCTCCAGCT
GTTCCAGACAATAGAAGACAGGCAGAAAGTCTTTCATTAACTAGGGAGGTTTCCCAGAGC
AGGAAACCATCAGCTTCAGAACGTTTACCTGATGAGAAAGTCGAACTTTTTAGCAAAATG
AGAGTGCTACAGGAAAAGAAACAAAAAATGGACAAATTGCTTGGAGAACTTCATACACTT
CGAGATCAGCATCTTAACAATTCATCATCCTCTCCACAAAGGAGTGTCGATCAGAGAAGT
ACTTCAGCTCCCTCTGCTTCTGTAGGCTTGGCACCGGTTGTCAATGGAGAATCCAATAGC
CTCACATCATCTGTTCCTTATCCTACTGCTTCTCTAGTATCTCAGAATGAGAGTGAAAAC
GAAGGCCACCTCAATCCATCTGAAAAACTCCAGAAGTTAAATGAAGTTCGAAAGAGATTG
AATGAGCTAAGAGAATTAGTTCATTATTATGAACAAACGTCAGACATGATGACAGATGCT
GTGAATGAAAACAGGAAAGATGAAGAAACTGAAGAGTCAGAATATGATTCTGAGCATGAA
AATTCCGAGCCTGTTACTAACATTCGAAATCCACAAGTAGCTTCCACTTGGAATGAAGTA
AATAGTCATAGTAATGCACAGTGTGTTTCTAATAATAGAGATGGGCGAACAGTTAATTCT
AATTGTGAAATTAACAACAGATCTGCTGCCAACATAAGGGCTCTAAACATGCCTCCTTCT
TTAGATTGTCGATATAATAGAGAAGGGGAACAGGAGATTCATGTTGCACAAGGTGAAGAT
GATGAGGAGGAGGAGGAAGAAGCAGAAGAGGAGGGAGTCAGTGGAGCTTCATTATCTAGT
CACAGGAGCAGTCTGGTTGATGAGCATCCAGAAGATGCTGAATTTGAACAGAAGATCAAC
CGACTTATGGCTGCAAAACAGAAACTTAGACAGTTACAAGATCTTGTTGCTATGGTACAG
GATGATGATGCAGCTCAAGGAGTTATCTCTGCCAGTGCATCAAATTTGGATGATTTCTAC
CCAGCAGAAGAAGACACCAAGCAAAATTCAAATAACACTAGAGGAAATGCCAATAAAACA
CAGAAAGATACTGGAGTAAATGAAAAGGCAAGAGAGAAATTTTATGAGGCTAAACTACAG
CAGCAACAGAGAGAGCTAAAACAATTGCAGGAAGAAAGAAAGAAACTGATTGACATTCAG
GAGAAAATTCAAGCATTGCAAACGGCATGCCCTGACTTACAGCTGTCAGCTGCTAGTGTG
GGTAACTGTCCCACCAAAAAATATATGCCAGCTGTTACTTCAACCCCAACTGTTAATCAA
CACGAGACCAGTACAAGCAAATCTGTTTTTGAGCCTGAAGATTCTTCAATAGTAGATAAT
GAGTTGTGGTCAGAAATGAGAAGACATGAAATGTTGAGGGAGGAGCTGCGACAGAGAAGA
AAGCAGCTTGAAGCTCTGATGGCTGAACATCAGAGGAGGCAAGGTCTAGCTGAAACTGCA
TCTCCAGTGGCTGTGTCATTGAGAAGTGATGGATCTGAGAACCTATGTACTCCTCAGCAA
AGTAGAACAGAAAAAACGATGGCAACTTGGGGAGGGTCTACCCAGTGTGCACTAGATGAA
GAAGGAGATGAAGACGGTTACCTTTCTGAAGGAATTGTTCGGACAGATGAAGAGGAGGAA
GAAGAGCAAGATGCCAGTTCCAATGATAACTTTTCTGTGTGTCCTTCTAACAGTGTGAAT
CATAACTCCTACAATGGAAAGGAAACTAAAAATAGGTGGAAGAACAATTGCCCTTTTTCG
GCAGATGAAAATTATCGTCCTTTAGCCAAGACAAGGCAACAGAATATCAGCATGCAACGG
CAAGAAAACCTTCGTTGGGTGTCAGAGCTCTCTTACGTAGAAGAGAAAGAACAATGGCAA
GAACAAATCAATCAGCTAAAGAAACAGCTTGATTTTAGTGTCAGTATTTGTCAGACTTTG
ATGCAAGACCAGCAGACTCTATCTTGTCTGCTACAAACTCTTCTCACGGGTCCTTACAGT
GTTATGCCCAGCAATGTTGCATCTCCTCAAGTACACTTCATAATGCACCAGTTGAACCAG
TGCTATACTCAGCTAACATGGCAACAGAATAATGTTCAGAGGTTGAAACAAATGCTAAAT
GAACTTATGCGCCAGCAAAATCAGCATCCAGAAAAACCTGGAGGCAAGGAAAGAGGCAGT
AGTGCATCGCACCCTCCTTCTCCCAGTTTATTTTGTCCTTTCAGCTTTCCAACACAGCCT
GTAAATCTCTTCAATATACCTGGATTTACTAACTTTTCATCATTTGCACCAGGTATGAAT
TTCAGCCCTTTATTTCCTTCTAATTTTGGAGATTTTTCTCAGAATATCTCTACACCCAGT
GAACAGCAGCAACCCTTAGCCCAGAATTCTTCAGGAAAAACAGAATATATGGCTTTTCCA
AAACCTTTTGAAAGCAGTTCCTCTATTGGAGCAGAGAAACCAAGGAATAAAAAACTGCCT
GAAGAGGAGGTGGAAAGCAGTAGGACACCATGGTTATATGAACAAGAAGGTGAAGTAGAG
AAACCATTTATCAAGACTGGATTTTCAGTGTCTGTAGAAAAATCTACAAGTAGTAACCGC
AAAAATCAATTAGATACAAACGGAAGAAGACGCCAGTTTGATGAAGAATCACTGGAAAGC
TTTAGCAGTATGCCTGATCCAGTAGATCCAACAACAGTGACTAAAACATTCAAGACAAGA
AAAGCGTCTGCACAGGCCAGCCTGGCATCTAAAGATAAAACTCCCAAGTCAAAAAGTAAG
AAGAGGAATTCTACTCAGCTGAAAAGCAGAGTTAAAAACATCAGGTATGAAAGTGCCAGT
ATGTCTAGCACATGTGAACCTTGCAAAAGTAGGAACAGACATTCAGCCCAGACTGAAGAG
CCTGTTCAAGCAAAAGTATTCAGCAGAAAGAATCATGAGCAACTGGAAAAAATAATAAAA
TGTAATAGGTCTACAGAAATATCTTCAGAAACTGGGAGTGATTTTTCCATGTTTGAAGCT
TTGCGAGATACTATTTATTCTGAAGTAGCTACATTAATTTCTCAAAATGAATCTCGTCCA
CATTTTCTTATTGAACTCTTCCATGAGCTGCAGCTACTAAACACAGACTACTTGAGACAG
AGGGCTTTATATGCATTGCAGGACATAGTATCCAGACATATTTCTGAGAGCCATGAAAAA
GGAGAAAATGTAAAGTCAGTAAACTCTGGTACTTGGATAGCATCAAACTCAGAACTTACT
CCTAGTGAGAGCCTTGCTACTACTGATGATGAAACTTTTGAGAAGAACTTTGAAAGAGAA
ACCCATAAAATAAGTGAGCAAAATGATGCTGATAATGCTAGTGTCCTGTCTGTATCATCA
AATTTTGAGCCTTTTGCAACAGATGATCTAGGTAACACCGTGATTCACTTAGATCAAGCA
TTAGCCAGAATGAGAGAATATGAGCGTATGAAGACTGAGGCTGAAAGTAACTCAAATATG
AGATGCACCTGCAGGATTATTGAGGATGGAGATGGTGCTGGTGCAGGTACTACAGTTAAT
AATTTAGAAGAAACTCCCGTTATTGAAAATCGTAGTTCACAACAACCTGTAAGTGAAGTT
TCTACCATCCCATGTCCTAGAATTGATACTCAGCAGCTGGACCGGCAAATTAAAGCAATT
ATGAAAGAAGTCATTCCTTTTTTGAAGGAGCACATGGATGAAGTATGCTCCTCGCAGCTT
CTAACTTCAGTAAGGCGCATGGTTTTGACCCTTACCCAGCAAAATGATGAGAGCAAAGAG
TTTGTAAAGTTCTTTCATAAACAACTTGGAAGTATATTACAGGATTCACTGGCAAAATTT
GCTGGCAGAAAACTGAAAGACTGTGGAGAAGATCTTCTTGTAGAGATATCTGAAGTGTTG
TTCAATGAATTGGCTTTCTTTAAGCTTATGCAAGATTTGGATAATAATAGTATAACTGTT
AAACAGAGATGCAAAAGGAAAATAGAAGCAACTGGAGTGATACAATCTTGTGCCAAAGAG
GCTAAAAGGATTCTTGAAGATCATGGCTCACCTGCTGGAGAGATTGATGATGAAGACAAA
GACAAGGATGAAACTGAAACAGTTAAGCAGACTCAAACATCTGAGGTGTATGATGGTCCC
AAAAATGTAAGATCTGATATTTCTGATCAAGAGGAAGATGAAGAAAGTGAAGGATGTCCA
GTGTCTATTAATTTGTCTAAAGCTGAAACTCAGGCTTTAACTAATTATGGAAGTGGAGAA
GATGAAAATGAGGATGAAGAAATGGAAGAATTTGAAGAAGGCCCTGTGGATGTCCAGACT
TCCCTCCAGGCTAACACTGAAGCTACTGAAGAAAATGAACATGATGAACAGGTCCTACAA
CGTGACTTTAAAAAGACAGCAGAAAGCAAAAATGTCCCATTGGAACGAGAAGCCACTAGT
AAAAATGACCAAAATAACTGTCCTGTGAAACCCTGTTACCTCAATATCTTGGAAGATGAG
CAACCTTTAAATAGTGCTGCCCATAAGGAGTCACCTCCTACTGTTGATTCAACTCAACAG
CCTAACCCTTTGCCGTTACGTTTACCTGAAATGGAACCCTTAGTGCCTAGAGTCAAAGAA
GTTAAATCTGCTCAGGAAACTCCTGAAAGCTCTCTGGCTGGAAGTCCTGATACTGAATCT
CCAGTGTTAGTGAATGACTATGAAGCAGAATCTGGTAATATAAGTCAAAAGTCTGATGAA
GAAGATTTTGTAAAAGTTGAAGATTTACCACTGAAACTGACAATATATTCAGAGGCAGAT
CTAAGAAAGAAAATGGTAGAAGAAGAACAGAAAAACCATTTATCTGGTGAAATATGTGAA
ATGCAGACCGAAGAATTAGCTGGAAATTCTGAGACACTAAAAGAACCTGAAACGGTGGGA
GCCCAGAGTATATTG
Protein sequence predicted based on nucleotide sequence
MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRV TNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSD SQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALS NDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLT HLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQ AALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPA VPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTL RDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESEN EGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHE NSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPS LDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKIN RLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKT QKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASV GNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRR KQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDE EGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFS ADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTL MQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLN ELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMN FSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLP EEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLES FSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESAS MSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEA LRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEK GENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSS NFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVN NLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQL LTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVL FNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDK DKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGE DENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATS KNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKE VKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEAD LRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSIL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
