Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859369
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6969nts
Open reading frame: 1 to 6969
ATGCAGTCCGGGCCGCGGCCCCCACTTCCAGCCCCCGGCCTGGCCTTGGCTTTGACCCTG
ACTATGTTGGCCAGACTTGCATCCGCGGCTTCCTTCTTCGGTGAGAACCACCTGGAGGTG
CCTGTGGCCACGGCTCTGACCGACATAGACCTGCAGCTGCAGTTCTCCACGTCCCAGCCC
GAAGCCCTCCTTCTCCTGGCAGCAGGCCCAGCTGACCACCTCCTGCTGCAGCTCTACTCT
GGACGCCTGCAGGTCAGACTTGTTCTGGGCCAGGAGGAGCTGAGGCTGCAGACTCCAGCA
GAGACGCTGCTGAGTGACTCCATCCCCCACACTGTGGTGCTGACTGTCGTAGAGGGCTGG
GCCACGTTGTCAGTCGATGGGTTTCTGAACGCCTCCTCAGCAGTCCCAGGAGCCCCCCTA
GAGGTCCCCTATGGGCTCTTTGTTGGGGGCACTGGGACCCTTGGCCTGCCCTACCTGAGG
GGAACCAGCCGACCCCTGAGGGGTTGCCTCCATGCAGCCACCCTCAATGGCCGCAGCCTC
CTCCGGCCTCTGACCCCCGATGTGCATGAGGGCTGTGCTGAAGAGTTTTCTGCCAGTGAT
GATGTGGCCCTGGGCTTCTCTGGGCCCCACTCTCTGGCTGCCTTCCCTGCCTGGGGCACT
CAGGACGAAGGAACCCTAGAGTTTACACTCACCACACAGAGCCGGCAGGCACCCTTGGCC
TTCCAGGCAGGGGGCCGGCGTGGGGACTTCATCTATGTGGACATATTTGAGGGCCACCTG
CGGGCCGTGGTGGAGAAGGGCCAGGGTACCGTATTGCTCCACAACAGTGTGCCTGTGGCC
GATGGGCAGCCCCATGAGGTCAGTGTCCACATCAATGCTCACCGGCTGGAAATCTCCGTG
GACCAGTACCCTACGCATACTTCGAACCGAGGAGTCCTCAGCTACCTGGAGCCACGGGGC
AGTCTCCTTCTCGGGGGGCTGGATGCAGAGGCCTCTCGTCACCTCCAGGAACACCGCCTG
GGCCTGACACCAGAGGCCACCAATGCCTCCCTGCTGGGCTGCATGGAAGACCTCAGTGTC
AATGGCCAGAGGCGGGGGCTGCGGGAAGCTTTGCTGACGCGCAACATGGCAGCCGGCTGC
AGGCTGGAGGAGGAGGAGTATGAGGACGATGCCTATGGACATTATGAAGCTTTCTCCACC
CTGGCCCCTGAGGCTTGGCCAGCCATGGAGCTGCCTGAGCCATGCGTGCCTGAGCCAGGG
CTGCCTCCTGTCTTTGCCAATTTCACCCAGCTGCTGACTATCAGCCCACTGGTGGTGGCC
GAGGGGGGCACAGCCTGGCTTGAGTGGAGGCATGTGCAGCCCACGCTGGACCTGATGGAG
GCTGAGCTGCGCAAATCCCAGGTGCTGTTCAGCGTGACCCGAGGGGCACGCCATGGCGAG
CTCGAGCTGGACATCCCGGGAGCCCAGGCACGAAAAATGTTCACCCTCCTGGACGTGGTG
AACCGCAAGGCCCGCTTCATCCACGATGGCTCTGAGGACACCTCCGACCAGCTGGTGCTG
GAGGTGTCGGTGACGGCTCGGGTGCCCATGCCCTCATGCCTTCGGAGGGGCCAAACATAC
CTCCTGCCCATCCAGGTCAACCCTGTCAATGACCCACCCCACATCATCTTCCCACATGGC
AGCCTCATGGTGATCCTGGAACACACGCAGAAGCCGCTGGGGCCTGAGGTTTTCCAGGCC
TATGACCCGGACTCTGCCTGTGAGGGCCTCACCTTCCAGGTCCTTGGCACCTCCTCTGGC
CTCCCCGTGGAGCGCCGAGACCAGCCTGGGGAGCCGGCGACCGAGTTCTCCTGCCGGGAG
TTGGAGGCCGGCAGCCTAGTCTATGTCCACCGCGGTGGTCCTGCACAGGACTTGACGTTC
CGGGTCAGCGATGGACTGCAGGCCAGCCCCCCGGCCACGCTGAAGGTGGTGGCCATCCGG
CCGGCCATACAGATCCACCGCAGCACAGGGTTGCGACTGGCCCAAGGCTCTGCCATGCCC
ATCTTGCCCGCCAACCTGTCGGTGGAGACCAATGCCGTGGGGCAGGATGTGAGCGTGCTG
TTCCGCGTCACTGGGGCCCTGCAGTTTGGGGAGCTGCAGAAGCAGGGGGCAGGTGGGGTG
GAGGGTGCTGAGTGGTGGGCCACACAGGCGTTCCACCAGCGGGATGTGGAGCAGGGCCGC
GTGAGGTACCTGAGCACTGACCCACAGCACCACGCTTACGACACCGTGGAGAACCTGGCC
CTGGAGGTGCAGGTGGGCCAGGAGATCCTGAGCAATCTGTCCTTCCCAGTGACCATCCAG
AGAGCCACTGTGTGGATGCTGCGGCTGGAGCCACTGCACACTCAGAACACCCAGCAGGAG
ACCCTCACCACAGCCCACCTGGAGGCCACCCTGGAGGAGGCAGGCCCAAGCCCCCCAACC
TTCCATTATGAGGTGGTTCAGGCTCCCAGGAAAGGCAACCTTCAACTACAGGGCACAAGG
CTGTCAGATGGCCAGGGCTTCACCCAGGATGACATACAGGCTGGCCGGGTGACCTATGGG
GCCACAGCACGTGCCTCAGAGGCAGTCGAGGACACCTTCCGTTTCCGTGTCACAGCTCCA
CCATATTTCTCCCCACTCTATACCTTCCCCATCCACATTGGTGGTGACCCAGATGCGCCT
GTCCTCACCAATGTCCTCCTCGTGGTGCCTGAGGGTGGTGAGGGTGTCCTCTCTGCTGAC
CACCTCTTTGTCAAGAGTCTCAACAGTGCCAGCTACCTCTATGAGGTCATGGAGCGGCCC
CGCCATGGGAGGTTGGCTTGGCGTGGGACACAGGACAAGACCACTATGGTGACATCCTTC
ACCAATGAAGACCTGTTGCGTGGCCGGCTGGTCTACCAGCATGATGACTCCGAGACCACA
GAAGATGATATCCCATTTGTTGCTACCCGCCAGGGCGAGAGCAGTGGTGACATGGCCTGG
GAGGAGGTACGGGGTGTCTTCCGAGTGGCCATCCAGCCCGTGAATGACCACGCCCCTGTG
CAGACCATCAGCCGGATCTTCCATGTGGCCCGGGGTGGGCGGCGGCTGCTGACTACAGAC
GACGTGGCCTTCAGCGATGCTGACTCGGGCTTTGCTGACGCCCAGCTGGTGCTTACCCGC
AAGGACCTCCTCTTTGGCAGTATCGTGGCCGTAGATGAGCCCACGCGGCCCATCTACCGC
TTCACCCAGGAGGACCTCAGGAAGAGGCGAGTACTGTTCGTGCACTCAGGGGCTGACCGT
GGCTGGATCCAGCTGCAGGTGTCCGACGGGCAACACCAGGCCACTGCGCTGCTGGAGGTG
CAGGCCTCGGAACCCTACCTCCGTGTGGCCAACGGCTCCAGCCTTGTGGTCCCTCAAGGA
GGCCAGGGCACCATCGACACGGCCGTGCTCCACCTGGACACCAACCTCGACATCCGCAGT
GGGGATGAGGTCCACTACCACGTCACAGCTGGCCCTCGCTGGGGACAGCTAGTCCGGGCT
GGTCAGCCAGCCACAGCCTTCTCCCAGCAGGACCTGCTGGATGGGGCCGTTCTCTATAGC
CACAATGGCAGCCTCAGCCCCCGCGACACCATGGCCTTCTCCGTGGAAGCAGGGCCAGTG
CACACGGATGCCACCCTACAAGTGACCATTGCCCTAGAGGGCCCACTGGCCCCACTGAAG
CTGGTCCGGCACAAGAAGATCTACGTCTTCCAGGGAGAGGCAGCTGAGATCAGAAGGGAC
CAGCTGGAGGCAGCCCAGGAGGCAGTGCCACCTGCAGACATCGTATTCTCAGTGAAGAGC
CCACCGAGTGCCGGCTACCTGGTGATGGTGTCGCGTGGCGCCTTGGCAGATGAGCCACCC
AGCCTGGACCCTGTGCAGAGCTTCTCCCAGGAGGCAGTGGACACAGGCAGGGTCCTGTAC
CTGCACTCCCGCCCTGAGGCCTGGAGCGATGCCTTCTCGCTGGATGTGGCCTCAGGCCTG
GGTGCTCCCCTCGAGGGCGTCCTTGTGGAGCTGGAGGTGCTGCCCGCTGCCATCCCACTA
GAGGCGCAAAACTTCAGCGTCCCTGAGGGTGGCAGCCTCACCCTGGCCCCTCCACTGCTC
CGTGTCTCCGGGCCCTACTTCCCCACTCTCCTGGGCCTCAGCCTGCAGGTGCTGGAGCCA
CCCCAGCATGGAGCCCTGCAGAAGGAGGACGGACCTCAAGCCAGGACCCTCAGCGCCTTC
TCCTGGAGAATGGTGGAAGAGCAGCTGATCCGCTACGTGCATGACGGGAGCGAGACACTG
ACAGACAGTTTTGTCCTGATGGCTAATGCCTCCGAGATGGATCGCCAGAGCCATCCTGTG
GCCTTCACTGTCACTGTCCTGCCTGTCAATGACCAACCCCCCATCCTCACTACAAACACA
GGCCTGCAGATGTGGGAGGGGGCCACTGCGCCCATCCCTGCGGAGGCTCTGAGGAGCACG
GACGGCGACTCTGGGTCTGAGGATCTGGTCTACACCATCGAGCAGCCCAGCAACGGGCGG
GTAGTGCTGCGGGGGGCGCCGGGCACTGAGGTGCGCAGCTTCACGCAGGCCCAGCTGGAC
GGCGGGCTCGTGCTGTTCTCACACAGAGGAACCCTGGATGGAGGCTTCCGCTTCCGCCTC
TCTGACGGCGAGCACACTTCCCCCGGACACTTCTTCCGAGTGACGGCCCAGAAGCAAGTG
CTCCTCTCGCTGAAGGGCAGCCAGACACTGACTGTCTGCCCAGGGTCCGTCCAGCCACTC
AGCAGTCAGACCCTCAGGGCCAGCTCCAGCGCAGGCACTGACCCCCAGCTCCTGCTCTAC
CGTGTGGTGCGGGGCCCCCAGCTAGGCCGGCTGTTCCACGCCCAGCAGGACAGCACAGGG
GAGGCCCTGGTGAACTTCACTCAGGCAGAGGTCTACGCTGGGAATATTCTGTATGAGCAT
GAGATGCCCCCCGAGCCCTTTTGGGAGGCCCATGATACCCTAGAGCTCCAGCTGTCCTCG
CCGCCTGCCCGGGACGTGGCCGCCACCCTTGCTGTGGCTGTGTCTTTTGAGGCTGCCTGT
CCCCAGCGCCCCAGCCACCTCTGGAAGAACAAAGGTCTCTGGGTCCCCGAGGGCCAGCGG
GCCAGGATCACCGTGGCTGCTCTGGATGCCTCCAATCTCTTGGCCAGCGTTCCATCACCC
CAGCGCTCAGAGCATGATGTGCTCTTCCAGGTCACACAGTTCCCCAGCCGGGGCCAGCTG
TTGGTGTCCGAGGAGCCCCTCCATGCTGGGCAGCCCCACTTCCTGCAGTCCCAGCTGGCT
GCAGGGCAGCTAGTGTATGCCCACGGCGGTGGGGGCACCCAGCAGGATGGCTTCCACTTT
CGTGCCCACCTCCAGGGGCCAGCAGGGGCCTCCGTGGCTGGACCCCAAACCTCAGAGGCC
TTTGCCATCACGGTGAGGGATGTAAATGAGCGGCCCCCTCAGCCACAGGCCTCTGTCCCA
CTCCGGCTCACCCGAGGCTCTCGTGCCCCCATCTCCCGGGCCCAGCTGAGTGTGGTGGAC
CCAGACTCAGCTCCTGGGGAGATTGAGTACGAGGTCCAGCGGGCACCCCACAACGGCTTC
CTCAGCCTGGTGGGTGGTGGCCTGGGGCCCGTGACCCGCTTCACGCAAGCCGATGTGGAT
TCAGGGCGGCTGGCCTTCGTGGCCAACGGGAGCAGCGTGGCAGGCATCTTCCAGCTGAGC
ATGTCTGATGGGGCCAGCCCACCCCTGCCCATGTCCCTGGCTGTGGACATCCTACCATCC
GCCATCGAGGTGCAGCTGCGGGCACCCCTGGAGGTGCCCCAAGCTTTGGGGCGCTCCTCA
CTGAGCCAGCAGCAGCTCCGGGTGGTTTCAGATCGGGAGGAGCCAGAGGCAGCATACCGC
CTCATCCAGGGACCCCAGTATGGGCATCTCCTGGTGGGCGGGCGGCCCACCTCGGCCTTC
AGCCAATTCCAGATAGACCAGGGCGAGGTGGTCTTTGCCTTCACCAACTTCTCCTCCTCT
CATGACCACTTCAGAGTCCTGGCACTGGCTAGGGGTGTCAATGCATCAGCCGTAGTGAAC
GTCACTGTGAGGGCTCTGCTGCATGTGTGGGCAGGTGGGCCATGGCCCCAGGGTGCCACC
CTGCGCCTGGACCCCACCGTCCTAGATGCTGGCGAGCTGGCCAACCGCACAGGCAGTGTG
CCGCGCTTCCGCCTCCTGGAGGGACCCCGGCATGGCCGCGTGGTCCGCGTGCCCCGAGCC
AGGACGGAGCCCGGGGGCAGCCAGCTGGTGGAGCAGTTCACTCAGCAGGACCTTGAGGAC
GGGAGGCTGGGGCTGGAGGTGGGCAGGCCAGAGGGGAGGGCCCCCGGCCCCGCAGGTGAC
AGTCTCACTCTGGAGCTGTGGGCACAGGGCGTCCCGCCTGCTGTGGCCTCCCTGGACTTT
GCCACTGAGCCTTACAATGCTGCCCGGCCCTACAGCGTGGCCCTGCTCAGTGTCCCCGAG
GCCGCCCGGACGGAAGCAGGGAAGCCAGAGAGCAGCACCCCCACAGGCGAGCCAGGCCCC
ATGGCATCCAGCCCTGAGCCCGCTGTGGCCAAGGGAGGCTTCCTGAGCTTCCTTGAGGCC
AACATGTTCAGCGTCATCATCCCCATGTGCCTGGTACTTCTGCTCCTGGCGCTCATCCTG
CCCCTGCTCTTCTACCTCCGAAAACGCAACAAGACGGGCAAGCATGACGTCCAGGTCCTG
ACTGCCAAGCCCCGCAACGGCCTGGCTGGTGACACCGAGACCTTTCGCAAGGTGGAGCCA
GGCCAGGCCATCCCGCTCACAGCTGTGCCTGGCCAGGGGCCCCCTCCAGGAGGCCAGCCT
GACCCAGAGCTGCTGCAGTTCTGCCGGACACCCAACCCTGCCCTTAAGAATGGCCAGTAC
TGGGTGTTG
Protein sequence predicted based on nucleotide sequence
MQSGPRPPLPAPGLALALTLTMLARLASAASFFGENHLEVPVATALTDIDLQLQFSTSQP EALLLLAAGPADHLLLQLYSGRLQVRLVLGQEELRLQTPAETLLSDSIPHTVVLTVVEGW ATLSVDGFLNASSAVPGAPLEVPYGLFVGGTGTLGLPYLRGTSRPLRGCLHAATLNGRSL LRPLTPDVHEGCAEEFSASDDVALGFSGPHSLAAFPAWGTQDEGTLEFTLTTQSRQAPLA FQAGGRRGDFIYVDIFEGHLRAVVEKGQGTVLLHNSVPVADGQPHEVSVHINAHRLEISV DQYPTHTSNRGVLSYLEPRGSLLLGGLDAEASRHLQEHRLGLTPEATNASLLGCMEDLSV NGQRRGLREALLTRNMAAGCRLEEEEYEDDAYGHYEAFSTLAPEAWPAMELPEPCVPEPG LPPVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLMEAELRKSQVLFSVTRGARHGE LELDIPGAQARKMFTLLDVVNRKARFIHDGSEDTSDQLVLEVSVTARVPMPSCLRRGQTY LLPIQVNPVNDPPHIIFPHGSLMVILEHTQKPLGPEVFQAYDPDSACEGLTFQVLGTSSG LPVERRDQPGEPATEFSCRELEAGSLVYVHRGGPAQDLTFRVSDGLQASPPATLKVVAIR PAIQIHRSTGLRLAQGSAMPILPANLSVETNAVGQDVSVLFRVTGALQFGELQKQGAGGV EGAEWWATQAFHQRDVEQGRVRYLSTDPQHHAYDTVENLALEVQVGQEILSNLSFPVTIQ RATVWMLRLEPLHTQNTQQETLTTAHLEATLEEAGPSPPTFHYEVVQAPRKGNLQLQGTR LSDGQGFTQDDIQAGRVTYGATARASEAVEDTFRFRVTAPPYFSPLYTFPIHIGGDPDAP VLTNVLLVVPEGGEGVLSADHLFVKSLNSASYLYEVMERPRHGRLAWRGTQDKTTMVTSF TNEDLLRGRLVYQHDDSETTEDDIPFVATRQGESSGDMAWEEVRGVFRVAIQPVNDHAPV QTISRIFHVARGGRRLLTTDDVAFSDADSGFADAQLVLTRKDLLFGSIVAVDEPTRPIYR FTQEDLRKRRVLFVHSGADRGWIQLQVSDGQHQATALLEVQASEPYLRVANGSSLVVPQG GQGTIDTAVLHLDTNLDIRSGDEVHYHVTAGPRWGQLVRAGQPATAFSQQDLLDGAVLYS HNGSLSPRDTMAFSVEAGPVHTDATLQVTIALEGPLAPLKLVRHKKIYVFQGEAAEIRRD QLEAAQEAVPPADIVFSVKSPPSAGYLVMVSRGALADEPPSLDPVQSFSQEAVDTGRVLY LHSRPEAWSDAFSLDVASGLGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPPLL RVSGPYFPTLLGLSLQVLEPPQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDGSETL TDSFVLMANASEMDRQSHPVAFTVTVLPVNDQPPILTTNTGLQMWEGATAPIPAEALRST DGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLDGGLVLFSHRGTLDGGFRFRL SDGEHTSPGHFFRVTAQKQVLLSLKGSQTLTVCPGSVQPLSSQTLRASSSAGTDPQLLLY RVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSS PPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSP QRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHF RAHLQGPAGASVAGPQTSEAFAITVRDVNERPPQPQASVPLRLTRGSRAPISRAQLSVVD PDSAPGEIEYEVQRAPHNGFLSLVGGGLGPVTRFTQADVDSGRLAFVANGSSVAGIFQLS MSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGRSSLSQQQLRVVSDREEPEAAYR LIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVN VTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRA RTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDF ATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEA NMFSVIIPMCLVLLLLALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEP GQAIPLTAVPGQGPPPGGQPDPELLQFCRTPNPALKNGQYWVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
