Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859547
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4671nts
Open reading frame: 1 to 4671
ATGCCGCTGCTACACCGAAAGCCGTTTGTGAGACAGAAGCCGCCCGCGGACCTGCGGCCC
GACGAGGAAGTTTTCTACTGTAAAGTCACCAACGAGATCTTCCGCCACTACGATGACTTT
TTTGAACGAACCATTCTGTGCAACAGCCTTGTGTGGAGTTGTGCTGTGACGGGTAGACCT
GGACTGACGTATCAGGAAGCACTTGAGTCAGAAAAAAAAGCAAGACAGAATCTTCAGAGT
TTTCCAGAACCACTAATTATTCCAGTTTTATACTTGACCAGCCTTACCCATCGTTCGCGC
TTACATGAAATTTGTGATGATATCTTTGCATATGTCAAGGATCGATATTTTGTCGAAGAA
ACTGTGGAAGTCATTAGGAACAATGGTGCAAGGTTGCAGTGTAGGATTTTGGAAGTCCTC
CCTCCATCACATCAAAATGGTTTTGCTAATGGACATGTTAACAGTGTGGATGGAGAAACT
ATTATCATCAGTGATAGTGATGATTCAGAAACACAAAGCTGTTCTTTTCAAAATGGGAAG
AAAAAAGATGCAATTGATCCCTTACTATTCAAGTATAAAGTGCAACCCACTAAAAAAGAA
TTACATGAGTCTGCTATTGTTAAAGCAACACAAATCAGCCGGAGAAAACACCTATTTTCT
CGTGATAAACTAAAGCTTTTTCTGAAGCAACACTGTGAACCACAAGATGGAGTCATTAAA
ATAAAGGCATCATCTCTTTCAACGTATAAAATAGCAGAACAAGATTTTTCTTATTTCTTC
CCTGATGATCCACCCACATTTATCTTCAGTCCTGCTAACAGACGAAGAGGGAGACCTCCC
AAACGAATACATATTAGTCAAGAGGACAATGTTGCTAATAAACAGACTCTTGCAAGTTAT
AGGAGCAAAGCTACTAAAGAAAGAGATAAACTTTTGAAACAAGAAGAAATGAAGTCACTG
GCTTTTGAAAAGGCTAAATTAAAAAGAGAAAAAGCAGATGCCCTAGAAGCGAAGAAAAAA
GAAAAAGAAGATAAAGAGAAAAAGAGGGAAGAATTGAAAAAAATTGTTGAAGAAGAGAGA
CTAAAGAAAAAAGAAGAAAAAGAGAGGCTTAAAGTAGAAAGAGAAAAGGAAAGAGAGAAG
TTACGTGAAGAAAAGCGAAAGTATGTGGAATACTTAAAACAGTGGAGTAAACCTAGAGAA
GATATGGAATGTGATGACCTTAAGGAACTTCCAGAACCAACACCAGTGAAAACTAGACTA
CCTCCTGAAATCTTTGGTGATGCTCTGATGGTTTTGGAGTTCCTTAATGCATTTGGGGAA
CTTTTTGATCTTCAAGATGAGTTTCCTGATGGAGTAACCCTAGAAGTATTAGAGGAAGCT
CTTGTAGGAAATGACAGTGAAGGCCCACTGTGTGAATTGCTTTTTTTCTTCCTGACTGCA
ATCTTCCAGGCAATAGCTGAAGAAGAAGAGGAAGTAGCCAAAGAGCAACTAACTGATGCT
GACACCAAAGATTTAACAGAGGCTTTGGATGAAGATGCAGACCCCACAAAATCTGCACTG
TCTGCAGTTGCATCTTTGGCAGCTGCATGGCCACAGTTACACCAGGGCTGCAGTTTGAAA
AGTTTGGATCTTGATAGCTGCACTCTTTCAGAAATCCTCAGACTGCACATCTTAGCTTCA
GGTGCTGATGTAACATCAGCAAATGCAAAGTATAGATATCAAAAACGAGGAGGATTTGAT
GCTACAGATGATGCTTGTATGGAGCTTCGTTTGAGCAATCCCAGTCTAGTGAAGAAACTG
TCAAGCACCTCAGTGTATGATTTGACACCAGGAGAAAAAATGAAGATACTCCATGCTCTC
TGTGGAAAGCTACTGACCCTAGTTTCAACTAGGGATTTTATTGAAGATTATGTTGATATA
TTACGACAGGCAAAGCAGGAGTTCCGGGAATTAAAAGCAGAACAACATCGAAAAGAGAGG
GAAGAAGCAGCTGCCAGAATTCGTAAAAGGAAGGAAGAAAAACTTAAGGAGCAAGAACAA
AAAATGAAAGAGAAACAAGAAAAACTGAAAGAAGATGAGCAAAGAAATTCAACGGCAGAT
ATATCTATTGGGGAGGAAGAAAGGGAAGATTTTGATACTAGCATTGAGAGCAAAGACACA
GAGCAAAAGGAATTAGATCAAGATATGGTCACTGAAGATGAAGATGACCCAGGATCACAT
AAAAGAGGCAGAAGGGGGAAAAGAGGACAAAATGGATTTAAAGAATTTACAAGGCAAGAA
CAGATCAACTGTGTAACAAGAGAGCCTCTTACTGCTGATGAGGAAGAAGCATTAAAACAG
GAACACCAACGAAAAGAGAAAGAGCTCTTAGAAAAAATCCAAAGTGCCATAGCCTGTACC
AATATCTTTCCCTTGGGTCGCGACCGCATGTATAGACGATACTGGATTTTCCCTTCTATT
CCTGGACTCTTTATTGAAGAGGATTATTCTGGTCTTACTGAAGACATGCTGTTGCCTAGA
CCTTCATCATTTCAGAATAATGTACAGTCTCAAGATCCTCAGGTATCCACTAAAACTGGA
GAGCCTTTGATGTCTGAATCTACCTCCAACATTGACCAAGGTCCACGTGACCATTCTGTG
CAGCTGCCAAAACCAGTGCATAAGCCAAATCGGTGGTGCTTTTACAGTTCTTGTGAACAG
CTAGACCAGCTTATTGAAGCTCTTAATTCTAGAGGACATAGAGAAAGTGCCTTAAAAGAA
ACTTTGTTACAAGAGAAAAGCAGAATATGTGCACAGCTAGCCCGTTTTTCTGAAGAGAAA
TTTCATTTTTCAGACAAACCTCAGCCTGATAGCAAACCAACATATAGTCGGGGAAGATCT
TCCAATGCATATGATCCATCTCAGATGTGTGCAGAAAAGCAACTTGAACTAAGGCTGAGA
GATTTTCTTTTAGATATTGAAGATAGAATCTACCAAGGAACATTAGGAGCCATCAAGGTT
ACAGATCGACATATCTGGAGATCAGCATTAGAAAGTGGACGGTATGAGCTGTTAAGTGAG
GAAAACAAGGAAAATGGGATAATTAAAACTGTGAATGAAGACGTAGAAGAGATGGAAATT
GATGAACAAACAAAGGTCATAGTAAAAGACAGACTTTTGGGGATAAAAACAGAAACTCCA
AGTACTGTATCAACAAATGCAAGTACACCACAATCAGTGAGCAGTGTGGTTCATTATCTG
GCAATGGCACTCTTTCAAATAGAGCAGGGCATTGAGCGGCGTTTTCTGAAAGCTCCACTT
GATGCCAGTGACAGTGGGCGTTCTTATAAAACAGTTCTGGACCGTTGGAGAGAGTCTCTC
CTTTCTTCTGCTAGTCTATCCCAAGTTTTTCTTCACCTATCCACCTTGGATCGTAGCGTG
ATATGGTCTAAATCTATACTGAATGCGCGTTGCAAGATATGTCGAAAGAAAGGCGATGCT
GAAAACATGGTTCTTTGTGATGGCTGTGATAGGGGTCATCATACCTACTGTGTTCGACCA
AAGCTCAAGACTGTGCCTGAAGGAGACTGGTTTTGTCCAGAATGTCGACCAAAGCAACGT
TCTAGAAGACTCTCCTCTAGACAGAGACCATCCTTGGAAAGTGATGAAGATGTGGAAGAC
AGTATGGGAGGTGAGGATGATGAAGTTGATGGCGATGAAGAAGAAGGTCAAAGTGAGGAG
GAAGAGTATGAGGTAGAACAAGATGAAGATGACTCTCAAGAAGAGGAAGAAGTCAGCCTA
CCCAAACGAGGAAGACCACAAGTTAGATTGCCAGTTAAAACAAGAGGGAAACTTAGCTCT
TCTTTCTCAAGTCGTGGCCAACAACAAGAACCTGGAAGATACCCTTCAAGGAGTCAGCAG
AGCACACCCAAAACAACTGTTTCTTCTAAAACTGGTAGAAGCCTAAGAAAGATAAACTCT
GCTCCTCCTACAGAAACAAAATCTTTAAGAATTGCCAGTCGTTCTACTCGCCACAGTCAT
GGCCCACTGCAAGCAGATGTATTTGTGGAATTGCTTAGTCCTCGTAGAAAACGCAGAGGC
AGGAAAAGTGCTAATAATACACCAGAAAATAGTCCCAACTTCCCTAACTTCAGAGTCATT
GCCACAAAGTCAAGTGAACAGTCAAGATCTGTAAATATTGCTTCAAAACTTTCTCTCCAA
GAGAGTGAATCCAAAAGAAGATGCAGAAAAAGACAATCTCCAGAGCCATCGCCTGTGACA
CTGGGTCGAAGGAGTTCTGGCCGACAGGGAGGAGTTCATGAATTGTCTGCTTTTGAACAA
CTTGTTGTAGAATTGGTACGACATGATGACAGCTGGCCTTTTTTGAAACTTGTTTCTAAA
ATCCAGGTCCCAGACTACTATGACATCATCAAAAAGCCCATTGCCTTAAATATAATTCGT
GAAAAAGTGAATAAGTGTGAATATAAATTAGCATCTGAGTTTATTGATGACATTGAGTTA
ATGTTTTCGAACTGCTTTGAATACAACCCTCGTAACACAAGTGAAGCAAAAGCTGGAACT
AGGCTTCAAGCATTTTTTCATATTCAGGCTCAAAAGCTTGGACTCCACGTCACACCCAGT
AATGTGGACCAAGTTAGCACACCACCGGCTGCGAAAAAGTCACGAATCTTG
Protein sequence predicted based on nucleotide sequence
MPLLHRKPFVRQKPPADLRPDEEVFYCKVTNEIFRHYDDFFERTILCNSLVWSCAVTGRP GLTYQEALESEKKARQNLQSFPEPLIIPVLYLTSLTHRSRLHEICDDIFAYVKDRYFVEE TVEVIRNNGARLQCRILEVLPPSHQNGFANGHVNSVDGETIIISDSDDSETQSCSFQNGK KKDAIDPLLFKYKVQPTKKELHESAIVKATQISRRKHLFSRDKLKLFLKQHCEPQDGVIK IKASSLSTYKIAEQDFSYFFPDDPPTFIFSPANRRRGRPPKRIHISQEDNVANKQTLASY RSKATKERDKLLKQEEMKSLAFEKAKLKREKADALEAKKKEKEDKEKKREELKKIVEEER LKKKEEKERLKVEREKEREKLREEKRKYVEYLKQWSKPREDMECDDLKELPEPTPVKTRL PPEIFGDALMVLEFLNAFGELFDLQDEFPDGVTLEVLEEALVGNDSEGPLCELLFFFLTA IFQAIAEEEEEVAKEQLTDADTKDLTEALDEDADPTKSALSAVASLAAAWPQLHQGCSLK SLDLDSCTLSEILRLHILASGADVTSANAKYRYQKRGGFDATDDACMELRLSNPSLVKKL SSTSVYDLTPGEKMKILHALCGKLLTLVSTRDFIEDYVDILRQAKQEFRELKAEQHRKER EEAAARIRKRKEEKLKEQEQKMKEKQEKLKEDEQRNSTADISIGEEEREDFDTSIESKDT EQKELDQDMVTEDEDDPGSHKRGRRGKRGQNGFKEFTRQEQINCVTREPLTADEEEALKQ EHQRKEKELLEKIQSAIACTNIFPLGRDRMYRRYWIFPSIPGLFIEEDYSGLTEDMLLPR PSSFQNNVQSQDPQVSTKTGEPLMSESTSNIDQGPRDHSVQLPKPVHKPNRWCFYSSCEQ LDQLIEALNSRGHRESALKETLLQEKSRICAQLARFSEEKFHFSDKPQPDSKPTYSRGRS SNAYDPSQMCAEKQLELRLRDFLLDIEDRIYQGTLGAIKVTDRHIWRSALESGRYELLSE ENKENGIIKTVNEDVEEMEIDEQTKVIVKDRLLGIKTETPSTVSTNASTPQSVSSVVHYL AMALFQIEQGIERRFLKAPLDASDSGRSYKTVLDRWRESLLSSASLSQVFLHLSTLDRSV IWSKSILNARCKICRKKGDAENMVLCDGCDRGHHTYCVRPKLKTVPEGDWFCPECRPKQR SRRLSSRQRPSLESDEDVEDSMGGEDDEVDGDEEEGQSEEEEYEVEQDEDDSQEEEEVSL PKRGRPQVRLPVKTRGKLSSSFSSRGQQQEPGRYPSRSQQSTPKTTVSSKTGRSLRKINS APPTETKSLRIASRSTRHSHGPLQADVFVELLSPRRKRRGRKSANNTPENSPNFPNFRVI ATKSSEQSRSVNIASKLSLQESESKRRCRKRQSPEPSPVTLGRRSSGRQGGVHELSAFEQ LVVELVRHDDSWPFLKLVSKIQVPDYYDIIKKPIALNIIREKVNKCEYKLASEFIDDIEL MFSNCFEYNPRNTSEAKAGTRLQAFFHIQAQKLGLHVTPSNVDQVSTPPAAKKSRIL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
