Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859657
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4551nts
Open reading frame: 1 to 4551
ATGGCGGCCAATGTGGGATCGATGTTTCAATATTGGAAGCGCTTTGATTTACAGCAGCTG
CAGAGAGAACTCGATGCCACCGCAACGGTATTGGCGAACCGGCAGGATGAAAGTGAGCAG
TCCAGAAAGCGGCTTATCGAACAGAGCCGGGAGTTCAAGAAGAACACTCCAGAGGATTTG
CGCAAGCAGGTAGCGCCGCTGCTGAAGAGTTTCCAAGGAGAGATTGATGCACTGAGTAAA
AGAAGCAAGGAAGCTGAAGCAGCTTTCTTGAATGTCTACAAAAGATTGATTGACGTCCCA
GATCCCGTACCAGCTTTGGATCTCGGACAGCAACTCCAGCTCAAAGTGCAGCGCCTGCAC
GATATTGAAACAGAGAACCAGAAACTTAGGGAAACTCTGGAAGAATACAACAAGGAATTT
GCTGAAGTGAAAAATCAAGAGGTTACGATAAAAGCACTTAAAGAGAAAATCCGAGAATAT
GAACAGACACTGAAGAACCAAGCCGAAACCATAGCTCTTGAGAAGGAACAGAAGTTACAG
AATGACTTTGCAGAAAAGGAGAGAAAGCTGCAGGAGACACAGATGTCCACCACCTCAAAG
CTGGAGGAAGCTGAGCATAAGGTTCAGAGCCTACAAACAGCCCTGGAAAAAACTCGAACA
GAATTATTTGACCTGAAAACCAAATACGATGAAGAAACTACTGCAAAGGCCGACGAGATT
GAAATGATCATGACGGACCTTGAAAGGGCAAACCAGAGGGCAGAGGTGGCTCAGAGAGAG
GCGGAGACCTTAAGGGAACAGCTCTCATCGGCCAATCACTCCCTCCAGCTGGCCTCACAG
ATCCAGAAGGCACCAGACGTGGAGCAGGCCATAGAGGTGCTGACCCGCTCCAGCCTAGAA
GTTGAGTTGGCCGCCAAGGAGCGGGAGATCGCACAGCTGGTGGAGGACGTGCAGAGACTC
CAGGCCAGCCTCACCAAGCTGCGGGAGAATTCGGCCAGCCAGATCTCACAGCTTGAGCAG
CAGCTGAGCGCCAAAAACAGCACACTCAAACAACTGGAAGAAAAACTCAAAGGCCAGGCT
GACTATGAAGAGGTGAAGAAAGAGCTGAACATTCTGAAGTCCATGGAGTTTGCACCGTCC
GAGGGCGCTGGGACACAGGATGCGGCCAAGCCCCTGGAGGTGCTGTTGCTGGAGAAGAAC
CGCTCGCTGCAGTCCGAGAACGCCGCGCTGCGCATCTCCAACAGCGACCTGAGCGGGTCA
GCCAGGAGGAAAGGGAAAGACCAGCCTGAAAGTCGGCGCCCGGGATCTTTGCCGGCCCCC
CCTCCTTCTCAGTTGCCCCGCAACCCGGGGGAGCAGGCTTCCAATACTAATGGTACACAC
CAGTTCTCACCAGCGGGGTTAAGTCAAGACTTTTTCAGCTCATCCCTGGCAAGCCCCAGC
CTACCCCTGGCTTCTACAGGAAAATTTGCACTAAACTCTCTTCTCCAGCGGCAGCTAATG
CAGTCCTTCTACTCCAAGGCTATGCAGGAAGCCGGAAGCACAAGCATGATTTTTTCAACA
GGTCCATACAGCACAAACTCCATATCTTCCCAAAGTCCATTACAACAAAGCCCAGATGTC
AATGGCATGGCCCCATCCCCCAGCCAGTCAGAAAGTGCTGGGAGCGTCTCCGAGGGCGAG
GAGATGGACACTGCAGAAATCGCCCGGCAGGTCAAAGAGCAGCTGATTAAGCACAATATC
GGACAACGTATTTTCGGACATTATGTGTTGGGACTGTCTCAAGGGTCCGTGAGCGAGATT
CTGGCCCGGCCCAAGCCATGGAATAAACTGACTGTTCGTGGCAAGGAGCCATTTCACAAG
ATGAAACAGTTCCTCTCCGATGAGCAGAACATCCTGGCCCTCCGTAGCATCCAAGGCAGA
CAAAGAGAGAATCCAGGCCAGAGCCTGAACAGACTATTTCAGGAAGTACCGAAACGAAGA
AATGGGTCTGAAGGTAACATCACCACCCGGATCCGAGCCTCGGAGACTGGCTCTGATGAA
GCCATCAAGTCCATCCTAGAGCAAGCCAAGAGGGAGCTCCAAGTGCAGAAAACTGCAGAG
CCGGCCCAGCCTTCCTCCGCATCCGGCAGCGGGAACTCTGATGACGCCATCCGCTCCATC
CTGCAGCAAGCCCGCCGGGAGATGGAGGCCCAGCAGGCTGCCCTCGACCCTGCCTTAAAG
CAGGCACCACTGTCCCAGAGTGACATCACCATCCTCACCCCCAAGCTTCTGTCCACCTCG
CCCATGCCCACCGTGTCCAGCTACCCACCTCTCGCCATCTCCCTGAAGAAGCCCTCCGCA
GCTCCTGAGGCCGGTGCCTCTGCTCTGCCGAACCCCCCGGCCCTCAAAAAGGAGGCCCAG
GACGCCCCCGGGCTGGACCCCCAGGGAGCAGCCGATTGTGCACAAGGGGTCCTGAGACAG
GTGAAAAATGAGGTGGGCCGCAGCGGTGCCTGGAAGGACCACTGGTGGAGCGCGGTGCAG
CCGGAGAGAAGAAATGCCGCCTCCTCCGAGGAGGCCAAGGCCGAAGAAACGGGCGGCGGG
AAAGAGAAGGGCAGCGGTGGCAGCGGAGGTGGCAGCCAGCCTCGGGCCGAGCGCAGTCAG
CTCCAGGGACCCTCGTCGTCAGAGTACTGGAAGGAGTGGCCCAGCGCTGAGTCCCCATAC
TCCCAGAGCTCAGAGCTGAGTCTGACCGGGGCCAGCCGCAGCGAGACACCACAGAACAGC
CCCCTGCCATCCTCCCCGATCGTGCCCATGTCCAAGCCCACCAAGCCCTCGGTCCCCCCG
CTGACCCCCGAGCAGTACGAGGTCTACATGTACCAGGAGGTGGACACCATCGAGCTCACC
CGGCAGGTTAAGGAAAAGCTGGCCAAGAACGGCATCTGCCAGAGAATCTTCGGGGAGAAG
GTGCTGGGCCTGTCCCAGGGCAGCGTCAGCGACATGCTGTCCCGACCGAAGCCATGGAGC
AAGCTGACGCAGAAAGGCCGAGAACCCTTCATCCGGATGCAGCTCTGGCTGAACGGCGAG
CTAGGCCAGGGTGTTCTACCCGTCCAGGGCCAGCAGCAAGGGCCAGTCCTCCACTCCGTG
ACATCGCTCCAGGACCCGCTGCAGCAGGGCTGTGTGAGCTCAGAAAGCACTCCAAAGACC
TCCGCCAGCTGCAGCCCTGCCCCTGAGTCCCCGATGAGTTCCAGTGAGTCGGTGAAGAGC
CTGACCGAGCTGGTCCAGCAGCCCTGTCCCCCCATCGAGGCGAGCAAGGACAGCAAGCCA
CCAGAGCCCAGTGACCCGCCAGCATCCGACTCCCAGCCCACAACCCCGCTGCCTCTCTCC
GGACACTCGGCCCTCAGCATCCAAGAATTAGTAGCCATGTCCCCGGAGCTGGACACCTAC
GGCATAACCAAGCGGGTGAAGGAGGTGCTGACGGACAACAACCTCGGCCAGCGCTTATTT
GGGGAGACCATCTTAGGGCTCACCCAAGGCTCTGTCTCTGACCTCCTTGCCCGCCCCAAA
CCCTGGCATAAGCTCAGTCTGAAAGGACGAGAGCCCTTCGTCCGGATGCAGCTGTGGCTG
AACGACCCCAACAATGTGGAGAAGCTGATGGACATGAAACGGATGGAGAAGAAAGCCTAC
ATGAAGCGGCGGCACAGCTCAGTCAGTGACAGCCAGCCCTGCGAACCGCCCTCTGTCGGC
ACCGAGTACAGCCAGGGCGCCAGCCCCCAGCCCCAGCACCAGCTGAAGAAACCCCGGGTG
GTGCTGGCTCCGGAGGAGAAGGAGGCGCTGAAACGAGCGTATCAGCAAAAGCCATACCCG
TCACCAAAAACCATCGAAGACCTCGCCACCCAGCTCAACCTGAAAACCAGCACCGTCATC
AACTGGTTCCACAACTACAGGTCTCGGATCCGCAGAGAACTGTTCATTGAGGAAATTCAG
GCCGGGAGTCAGGGCCAGGCGGGCGCCAGCGACTCACCCTCGGCCCGCAGCGGCCGGGCG
GCGCCCAGCTCGGAGGGCGACAGCTGCGACGGCGTGGAGGCCACTGAGGGCCCAGGCAGC
GCCGACACCGAGGAGCCCAAGTCTCAGGGAGAGGCCGAGCGGGAGGAGGTGCCGCGGCCG
GCGGAGCAGACGGAGCCGCCGCCCTCGGGGACCCCGGGCCCGGACGACGCCCGCGACGAC
GACCACGAGGGAGGCCCCGTGGAAGGCCCGGGGCCCCTGCCCAGCCCCGCCTCCGCGACC
GCCACCGCCGCGCCCGCGGCCCCCGAGGACGCCGCTACCTCAGCCGCCGCCGCGCCGGGG
GAGGGCCCCGCGGCCCCGAGCTCCGCGCCGCCGCCCAGCAACAGCAGCAGCAGCAGCGCC
CCCCGCAGGCCCAGCTCGCTGCAGAGCCTTTTCGGCCTCCCCGAGGCCGCGGGCGCCCGG
GACTCGCGCGACAACCCCCTGCGCAAGAAGAAGGCCGCGAACTTGAACAGCATCATCCAC
CGCCTGGAGAAGGCCGCCAGCCGGGAGGAACCTATCGAATGGGAGTTCTTG
Protein sequence predicted based on nucleotide sequence
MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDL RKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLH DIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQ NDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEI EMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLE VELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQA DYEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALRISNSDLSGS ARRKGKDQPESRRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQDFFSSSLASPS LPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDV NGMAPSPSQSESAGSVSEGEEMDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEI LARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRR NGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASGSGNSDDAIRSI LQQARREMEAQQAALDPALKQAPLSQSDITILTPKLLSTSPMPTVSSYPPLAISLKKPSA APEAGASALPNPPALKKEAQDAPGLDPQGAADCAQGVLRQVKNEVGRSGAWKDHWWSAVQ PERRNAASSEEAKAEETGGGKEKGSGGSGGGSQPRAERSQLQGPSSSEYWKEWPSAESPY SQSSELSLTGASRSETPQNSPLPSSPIVPMSKPTKPSVPPLTPEQYEVYMYQEVDTIELT RQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGE LGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKS LTELVQQPCPPIEASKDSKPPEPSDPPASDSQPTTPLPLSGHSALSIQELVAMSPELDTY GITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWL NDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGTEYSQGASPQPQHQLKKPRV VLAPEEKEALKRAYQQKPYPSPKTIEDLATQLNLKTSTVINWFHNYRSRIRRELFIEEIQ AGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEATEGPGSADTEEPKSQGEAEREEVPRP AEQTEPPPSGTPGPDDARDDDHEGGPVEGPGPLPSPASATATAAPAAPEDAATSAAAAPG EGPAAPSSAPPPSNSSSSSAPRRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIH RLEKAASREEPIEWEFL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
