Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859678
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4494nts
Open reading frame: 1 to 4494
ATGGATGTAACCAAGAAAAACAAACGAGATGGAACTGAAGTCACTGAGAGAATTGTCACT
GAAACAGTAACCACAAGACTTACATCCTTACCACCAAAAGGCGGGACCAGCAATGGCTAT
GCTAAAACAGCCTCTCTTGGTGGAGGGAGCCGGCTGGAGAAACAAAGCCTGACTCATGGC
AGCAGCGGCTACATAAACTCAACTGGAAGCACACGAGGCCATGCCTCCACCTCTAGTTAC
AGGAGGGCTCACTCACCTGCCTCCACTCTGCCCAACTCCCCAGGCTCAACCTTTGAAAGG
AAAACTCACGTTACCCGCCATGCGTATGAAGGGAGCTCCAGTGGCAACTCTTCTCCGGAG
TACCCTCGGAAGGAATTTGCATCTTCTTCAACCAGAGGACGGAGTCAAACACGAGAGAGT
GAAATTCGAGTTCGACTGCAGAGTGCGTCCCCATCCACCCGATGGACAGAATTGGATGAT
GTTAAGCGTTTGCTCAAGGGGAGTCGATCGGCAAGTGTGAGCCCCACCCGGAATTCCTCC
AACACACTCCCCATCCCCAAGAAAGGCACTGTGGAGACCAAAATTGTGACAGCGAGCTCC
CAGTCGGTGTCAGGCACCTACGATGCAACGATCCTGGATGCCAACCTTCCCTCCCATGTG
TGGTCCTCCACCCTGCCCGCGGGGTCCTCCATGGGGACCTATCACAACAACATGACAACC
CAGAGCTCATCCCTCCTCAACACCAATGCCTACTCTGCGGGATCAGTCTTCGGAGTTCCA
AACAACATGGCGTCCTGCTCACCCACTTTGCACCCTGGACTCAGCACATCCTCCTCAGTG
TTTGGCATGCAGAACAATCTGGCCCCCAGCTTGACCACCCTGTCCCATGGCACCACCACC
ACTTCCACAGCATATGGGGTGAAGAAAAACATGCCCCAGAGTCCTGCGGCTGTGAACACT
GGCGTTTCCACCTCCGCCGCCTGCACCACAAGTGTGCAGAGCGATGACCTTTTGCACAAG
GACTGCAAGTTCCTGATCCTAGAGAAAGACAACACACCTGCCAAGAAGGAGATGGAGCTG
CTCATCATGACCAAGGACAGCGGGAAGGTCTTTACAGCCTCCCCTGCCAGCATCGCTGCA
ACTTCTTTTTCAGAAGACACCCTAAAAAAAGAAAAGCAAGCTGCCTACAATGCTGACTCA
GGCCTAAAAGCCGAAGCTAATGGAGACCTGAAGACTGTGTCCACAAAGGGCAAGACCACC
ACTGCAGATATCCACAGCTACGGCAGCAGTGGTGGTGGTGGCAGTGGAGGAGGTGGCGGT
GTTGGTGGCGCTGGCGGCGGCCCTTGGGGACCAGCGCCAGCCTGGTGCCCCTGCGGCTCC
TGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACCTGGCTGCTACTCCTGGGG
CTGCTCTTCGGCCTCATTGCTCTGGCGGAGGAGGTGAGGAAGCTGAAGGCGCGTGTGGAT
GAGCTGGAGAGGATCAGGAGGAGCATACTGCCCTATGGGGACAGCATGGATAGAATAGAA
AAGGACCGCCTCCAGGGCATGGCACCCGCGGCGGGAGCAGACCTGGACAAAATTGGGCTG
CACAGTGACAGCCAGGAGGAGCTCTGGATGTTCGTGAGGAAGAAGCTAATGATGGAACAG
GAAAATGGAAATCTCCGAGGAAGCCCTGGCCCTAAAGGTGACATGGGAAGTCCAGGCCCT
AAAGGAGATCGAGGGTTCCCTGGGACTCCAGGTATCCCTGGGCCCTTGGGCCACCCAGGT
CCACAAGGACCAAAGGGTCAAAAAGGCAGCGTGGGAGATCCTGGCATGGAAGGCCCCATG
GGCCAGAGAGGGCGAGAAGGCCCCATGGGACCTCGTGGTGAGGCAGGGCCTCCTGGATCT
GGAGAGAAAGGGGAAAGAGGGGCTGCTGGTGAACCAGGTCCTCATGGCCCACCTGGTGTC
CCAGGTTCTGTGGGTCCCAAAGGTTCCAGCGGCTCTCCTGGCCCACAGGGCCCTCCAGGT
CCTGTAGGTCTCCAAGGGCTCCGAGGTGAAGTAGGACTTCCTGGTGTCAAAGGTGACAAA
GGACCAATGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAAGGACCTCGAGGCCTC
ACAGGCGAGCCTGGCATGAGAGGTTTGCCTGGTGCTGTTGGTGAGCCCGGGGCTAAAGGA
GCAATGGGTCCTGCTGGCCCAGACGGACACCAAGGCCCAAGAGGTGAACAAGGTCTTACT
GGGATGCCTGGAATCCGTGGCCCACCAGGACCTTCTGGAGACCCAGGAAAGCCAGGTCTC
ACAGGACCCCAGGGACCTCAGGGACTTCCCGGTACCCCTGGCCGACCAGGAATAAAAGGT
GAACCAGGAGCTCCAGGCAAGATCGTGACTTCGGAGGGGTCATCGATGCTCACTGTCCCA
GGCCCCCCAGGACCTCCTGGAGCCATGGGACCCCCAGGACCTCCAGGTGCCCCAGGCCCT
GCCGGCCCAGCTGGTCTCCCAGGACATCAAGAAGTTCTTAATTTACAAGGTCCCCCAGGC
CCACCCGGCCCACGCGGGCCACCAGGGCCTTCCATTCCAGGCCCACCAGGACCCCGAGGC
CCACCAGGGGAGGGTTTGCCAGGCCCACCAGGCCCACCAGGATCGTTCCTGTCCAACTCA
GAAACCTTCCTCTCCGGCCCCCCAGGCCCACCTGGCCCCCCAGGTCCCAAGGGAGACCAA
GGTCCCCCAGGCCCCAGAGGACACCAAGGCGAGCAAGGCCTCCCAGGTTTCTCAACCTCA
GGGTCCAGTTCTTTCGGACTCAACCTTCAGGGACCACCAGGCCCACCTGGCCCCCAGGGA
CCCAAAGGTGACAAAGGTGATCCAGGTGTTCCAGGGGCTCTTGGCATTCCTAGTGGTCCT
TCTGAAGGGGGATCATCAAGTACCATGTACGTGTCAGGCCCGCCAGGGCCCCCTGGGCCC
CCTGGGCCTCCGGGCTCTATCAGCAGCTCTGGCCAGGAGATTCAGCAGTACATCTCTGAG
TACATGCAGAGTGACAGTATTAGATCTTACCTATCCGGAGTTCAGGGTCCCCCAGGCCCA
CCTGGTCCCCCAGGACCTGTCACCACCATCACAGGCGAGACTTTCGACTACTCAGAGCTG
GCAAGCCACGTTGTGAGCTACTTACGGACTTCGGGGTACGGTGTCAGCTTGTTCTCGTCC
TCCATCTCTTCTGAAGACATTCTGGCTGTGCTGCAGCGGGATGACGTGCGTCAGTACCTA
CGTCAGTACTTGATGGGCCCTCGGGGTCCGCCAGGGCCACCAGGAGCCAGTGGAGATGGG
TCCCTCCTGTCTTTGGACTATGCAGAGCTGAGTAGTCGCATTCTCAGCTACATGTCGAGT
TCTGGGATCAGCATTGGGCTTCCTGGTCCCCCGGGGCCCCCTGGCTTGCCGGGAACCTCC
TATGAGGAGCTCCTCTCCTTGCTGCGAGGGTCTGAATTCAGAGGCATCGTTGGACCCCCA
GGTCCCCCGGGTCCACCAGGGATCCCAGGCAATGTGTGGTCCAGCATCAGCGTGGAGGAC
CTCTCGTCTTACTTACATACTGCCGGCTTGTCATTCATCCCAGGCCCTCCAGGACCTCCT
GGTCCCCCAGGGCCTCGAGGGCCCCCGGGTGTCTCAGGAGCCCTGGCAACCTATGCAGCT
GAAAACAGCGACAGCTTCCGGAGCGAGCTGATCAGCTACCTCACAAGTCCTGATGTGCGC
AGCTTCATTGTTGGCCCCCCAGGCCCTCCTGGGCCGCAGGGACCCCCTGGGGACAGCCGC
CTCCTGTCCACGGATGCCTCCCACAGTCGGGGTAGCAGCTCCTCCTCACACAGCTCATCT
GTCAGGCGGGGCAGCTCCTACAGCTCTTCCATGAGCACAGGAGGAGGTGGTGCAGGCTCC
CTGGGTGCAGGCGGTGCCTTTGGTGAAGCTGCAGGAGACAGGGGTCCCTATGGCACTGAC
ATCGGCCCAGGCGGAGGCTATGGGGCAGCAGCAGAAGGCGGCATGTATGCTGGCAATGGC
GGACTATTGGGAGCTGACTTTGCTGGAGATCTGGATTACAATGAGCTGGCTGTGAGGGTG
TCAGAGAGCATGCAGCGTCAGGGCCTACTGCAAGGGATGGCCTACACTGTCCAGGGCCCA
CCAGGCCAGCCTGGGCCACAGGGGCCACCCGGCATCAGCAAGGTCTTCTCTGCCTACAGC
AACGTGACTGCGGACCTCATGGACTTCTTCCAAACTTATGGAGCCATTCAAGGACCCCCT
GGGCAAAAAGGAGAGATGGGCACTCCAGGACCCAAAGGTGACAGGGGCCCTGCTGGGCCA
CCAGGTCATCCTGGGCCACCTGGCCCTCGAGGACACAAGGGAGAAAAAGGAGACAAAGGT
GACCAAGTCTATGCTGGGCGGAGAAGGAGAAGAAGTATTGCTGTCAAGCCGTTG
Protein sequence predicted based on nucleotide sequence
MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHG SSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPE YPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSS NTLPIPKKGTVETKIVTASSQSVSGTYDATILDANLPSHVWSSTLPAGSSMGTYHNNMTT QSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSVFGMQNNLAPSLTTLSHGTTT TSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKDNTPAKKEMEL LIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTT TADIHSYGSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLG LLFGLIALAEEVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGL HSDSQEELWMFVRKKLMMEQENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPG PQGPKGQKGSVGDPGMEGPMGQRGREGPMGPRGEAGPPGSGEKGERGAAGEPGPHGPPGV PGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDKGPMGPPGPKGDQGEKGPRGL TGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPGPSGDPGKPGL TGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGP AGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNS ETFLSGPPGPPGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQG PKGDKGDPGVPGALGIPSGPSEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISE YMQSDSIRSYLSGVQGPPGPPGPPGPVTTITGETFDYSELASHVVSYLRTSGYGVSLFSS SISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDGSLLSLDYAELSSRILSYMSS SGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPGNVWSSISVED LSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVR SFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGS LGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRV SESMQRQGLLQGMAYTVQGPPGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPP GQKGEMGTPGPKGDRGPAGPPGHPGPPGPRGHKGEKGDKGDQVYAGRRRRRSIAVKPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
