Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859680
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5517nts
Open reading frame: 1 to 5517
ATGGACGTCCATACCCGCTGGAAAGCGCGCAGCGCGCTCCGCCCGGGCGCCCCGCTGCTG
CCCCCGCTGCTGCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAGCA
GATCTCCTGAAGGTTCTAGATTTTCACAACTTGCCTGATGGAATAACAAAGACAACAGGC
TTTTGCGCCACGCGGCGATCTTCCAAAGGCCCGGATGTCGCTTACAGAGTCACCAAAGAC
GCGCAGCTCAGCGCACCCACCAAGCAGCTGTACCCTGCGTCTGCATTTCCCGAGGACTTC
TCCATCCTAACAACTGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTGGTCTCCATCTAC
AACGAGCAGGGTATCCAGCAGATTGGGCTGGAGCTGGGCCGCTCTCCCGTCTTCCTCTAC
GAGGACCACACGGGGAAGCCTGGCCCGGAAGACTACCCCCTCTTCCGGGGCATCAACCTG
TCAGATGGCAAGTGGCACAGAATTGCTCTCAGCGTCCACAAGAAAAATGTCACCTTGATC
CTCGACTGTAAAAAGAAGACCACCAAATTCCTCGACCGCAGCGACCACCCCATGATCGAC
ATCAATGGCATCATCGTGTTTGGCACCCGGATCCTGGATGAGGAGGTGTTTGAGGGTGAC
ATCCAGCAGCTGCTCTTTGTCTCGGACCACCGGGCAGCTTATGATTACTGTGAGCACTAC
AGCCCTGACTGTGACACCGCAGTACCTGACACCCCACAGTCGCAGGACCCCAATCCAGAT
GAATATTACACGGAAGGAGACGGCGAGGGTGAGACCTATTACTACGAATACCCCTACTAC
GAAGACCCCGAAGACCTAGGGAAGGAGCCCACCCCCAGCAAGAAGCCCGTGGAAGCTGCC
AAAGAAACCACAGAGGTCCCCGAGGAGCTGACCCCGACCCCCACGGAAGCTGCTCCCATG
CCTGAAACCAGTGAAGGGGCTGGGAAGGAAGAGGACGTCGGCATCGGGGACTATGACTAC
GTGCCCAGTGAGGACTACTACACGCCCTCACCGTATGATGACCTCACCTATGGCGAGGGG
GAGGAGAACCCCGACCAGCCCACAGACCCAGGCGCTGGGGCCGAAATTCCCACCAGCACC
GCCGACACCTCCAACTCCTCCAATCCAGCTCCGCCTCCAGGGGAAGGTGCGGATGACTTG
GAGGGGGAGTTCACTGAGGAAACGATCCGGAACCTTGACGAGAACTACTACGACCCCTAC
TACGACCCCACCAGCTCCCCGTCGGAGATCGGGCCGGGAATGCCGGCGAACCAGGATACC
ATCTATGAAGGGATTGGAGGACCTCGGGGCGAGAAAGGCCAAAAGGGAGAACCAGCGATT
ATCGAGCCGGGCATGCTCATCGAGGGCCCGCCTGGCCCAGAAGGCCCCGCGGGTCTTCCC
GGACCTCCAGGAACCATGGGTCCCACTGGCCAAGTCGGGGACCCTGGAGAAAGGGGCCCC
CCTGGACGCCCAGGCCTTCCTGGGGCCGATGGCCTGCCCGGTCCTCCAGGAACCATGCTC
ATGCTGCCCTTCCGGTTTGGAGGTGGCGGCGATGCGGGCTCCAAAGGCCCCATGGTCTCA
GCCCAGGAGTCCCAGGCGCAAGCCATTCTCCAGCAGGCCAGGTTGGCACTGAGGGGACCA
GCTGGCCCGATGGGTCTCACAGGGAGACCTGGCCCTGTGGGTCCCCCTGGGAGCGGAGGT
TTGAAGGGCGAGCCGGGAGACGTGGGGCCTCAGGGTCCTCGAGGTGTGCAAGGCCCGCCT
GGTCCGGCCGGGAAGCCCGGAAGACGGGGTCGGGCTGGGAGTGATGGAGCCAGAGGAATG
CCTGGACAAACTGGCCCCAAGGGTGACCGGGGTTTCGACGGCCTGGCTGGGTTGCCAGGC
GAGAAGGGCCACAGGGGTGACCCTGGTCCTTCCGGCCCACCAGGACCTCCGGGAGACGAT
GGAGAAAGGGGTGACGACGGAGAAGTTGGGCCCAGGGGGCTGCCTGGGGAGCCCGGGCCA
CGTGGTCTGCTTGGGCCGAAGGGGCCCCCAGGTCCTCCCGGACCTCCCGGTGTCACGGGT
ATGGACGGCCAGCCGGGGCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGCCCCCCA
GGACAGCAGGGTAATCCAGGCGCCCAGGGTCTTCCAGGCCCCCAGGGTGCAATTGGTCCT
CCAGGAGAAAAGGGTCCCTTGGGGAAACCAGGCCTTCCAGGAATGCCCGGTGCTGACGGA
CCCCCGGGACACCCTGGCAAAGAAGGCCCTCCAGGAGAGAAAGGAGGTCAGGGTCCACCT
GGCCCCCAGGGTCCGATTGGCTACCCAGGTCCTCGAGGAGTCAAGGGGGCCGATGGCATC
CGTGGTCTGAAGGGCACAAAGGGCGAGAAGGGTGAAGACGGCTTTCCTGGGTTTAAAGGA
GACATGGGCATCAAGGGTGATCGGGGGGAGATCGGCCCACCCGGTCCCAGGGGAGAAGAT
GGCCCTGAAGGCCCAAAGGGTCGCGGAGGTCCCAATGGTGACCCCGGTCCTCTGGGACCC
CCTGGGGAGAAGGGAAAACTCGGAGTCCCAGGGTTACCAGGGTATCCAGGAAGACAAGGA
CCAAAGGGCTCTATTGGATTCCCTGGATTTCCTGGCGCCAATGGAGAGAAGGGCGGCAGG
GGGACCCCTGGAAAGCCAGGACCGCGGGGGCAGCGAGGCCCAACGGGTCCGAGGGGTGAA
AGAGGCCCCCGGGGCATCACTGGGAAGCCTGGCCCCAAGGGCAACTCCGGAGGTGACGGC
CCAGCTGGCCCTCCTGGTGAACGGGGACCCAATGGACCCCAAGGACCCACAGGATTTCCT
GGACCAAAGGGCCCCCCTGGCCCTCCAGGCAAGGATGGACTCCCAGGACACCCTGGACAG
AGAGGCGAGACTGGTTTCCAAGGCAAGACCGGCCCTCCAGGCCCCCCCGGCGTGGTCGGC
CCTCAGGGTCCCACGGGAGAAACGGGCCCAATGGGTGAGCGTGGCCACCCTGGGCCCCCT
GGACCCCCCGGTGAACAGGGGCTTCCGGGCCTTGCTGGAAAAGAAGGGACGAAGGGTGAC
CCAGGCCCTGCAGGCCTCCCTGGGAAAGATGGCCCTCCAGGATTACGTGGTTTCCCTGGG
GACCGAGGGCTTCCTGGTCCAGTGGGAGCTCTTGGACTGAAAGGCAATGAAGGGCCCCCT
GGCCCACCAGGCCCTGCGGGATCTCCAGGGGAGAGAGGTCCAGCTGGAGCCGCTGGGCCC
ATCGGAATTCCAGGGAGACCTGGGCCCCAGGGACCCCCAGGGCCGGCAGGAGAGAAAGGG
GCTCCTGGCGAGAAAGGCCCACAAGGCCCAGCTGGCCGAGACGGTCTCCAGGGGCCTGTG
GGGCTCCCGGGTCCAGCTGGCCCTGTGGGTCCCCCTGGAGAAGACGGAGATAAGGGAGAG
ATCGGGGAGCCGGGGCAGAAAGGAAGCAAGGGGGACAAAGGAGAACAGGGTCCTCCTGGG
CCTACAGGTCCTCAAGGCCCCATCGGACAGCCAGGCCCCTCTGGAGCTGACGGCGAGCCG
GGGCCTCGGGGCCAGCAGGGCCTTTTCGGGCAGAAAGGTGATGAAGGTCCCAGAGGCTTT
CCTGGACCCCCTGGGCCAGTGGGGCTGCAGGGTTTGCCAGGACCTCCAGGCGAGAAGGGT
GAGACAGGAGACGTGGGCCAGATGGGCCCCCCGGGTCCCCCTGGCCCCCGAGGACCCTCC
GGAGCTCCAGGTGCTGATGGCCCACAAGGTCCCCCAGGTGGAATAGGAAACCCTGGTGCA
GTGGGAGAGAAGGGCGAGCCTGGCGAAGCAGGTGAGCCTGGCCTTCCGGGAGAAGGCGGC
CCCCCGGGACCCAAAGGAGAAAGGGGAGAGAAGGGCGAGTCAGGCCCTTCAGGTGCTGCC
GGACCCCCTGGACCCAAAGGCCCTCCCGGAGATGATGGTCCCAAAGGCAGCCCTGGCCCA
GTGGGTTTTCCTGGAGATCCTGGCCCCCCCGGAGAGCCTGGCCCCGCGGGTCAAGATGGT
CCCCCTGGTGACAAAGGAGATGATGGTGAACCCGGGCAGACGGGATCCCCCGGCCCTACT
GGTGAACCAGGTCCATCGGGGCCTCCAGGAAAAAGGGGTCCCCCAGGCCCCGCAGGCCCC
GAAGGCAGACAGGGAGAGAAAGGGGCCAAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGG
AAGACTGGCCCCATCGGCCCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGA
GGGATCCCTGGCCCTGTGGGAGAACAAGGTCTCCCAGGATCCCCAGGCCCGGACGGTCCC
CCCGGCCCCATGGGTCCCCCAGGACTTCCCGGCCTCAAAGGAGATTCTGGTCCCAAAGGT
GAAAAGGGTCATCCAGGCCTGATCGGGCTCATCGGTCCTCCGGGTGAACAGGGTGAGAAG
GGCGACCGTGGTCTCCCTGGCCCCCAGGGCTCCTCCGGTCCTAAGGGAGAACAGGGTATC
ACTGGTCCTTCTGGCCCGATTGGGCCTCCTGGGCCCCCTGGCCTGCCGGGTCCGCCTGGT
CCAAAAGGTGCTAAGGGCTCCTCGGGTCCAACTGGCCCGAAGGGTGAGGCAGGCCACCCA
GGACCCCCAGGCCCCCCGGGCCCCCCGGGAGAGGTCATCCAGCCCCTGCCAATCCAGGCA
TCCAGGACGCGGCGGAACATCGACGCCAGCCAGCTGCTGGACGACGGGAATGGCGAGAAC
TACGTGGACTACGCGGACGGCATGGAAGAGATCTTCGGCTCTCTCAACTCTCTGAAGCTG
GAGATTGAGCAGATGAAACGGCCCCTGGGCACGCAGCAGAACCCCGCCCGCACCTGCAAG
GACCTGCAGCTCTGCCACCCCGACTTCCCAGATGGTGAATACTGGGTCGATCCTAACCAA
GGATGCTCCAGGGATTCCTTCAAGGTTTACTGCAACTTCACAGCCGGGGGGTCGACATGC
GTCTTCCCTGACAAGAAGTCCGAAGGGAGTAAAATGGCCCGCTGGCCCAAAGAGCAGCCT
TCCACCTGGTATAGTCAGTACAAGCGGGGGTCCCTGCTCTCCTATGTGGACGCCGAGGGC
AACCCTGTGGGTGTGGTACAGATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAG
AACGTCACCTACCACTGCTACCAGTCAGTGGCCTGGCAGGACGCAGCCACGGGCAGCTAC
GACAAGGCCCTCCGCTTCCTGGGCTCCAACGACGAGGAGATGTCCTATGACAACAACCCC
TACATCCGCGCCCTGGTGGACGGCTGTGCTACCAAGAAAGGCTACCAGAAGACGGTTCTG
GAGATCGACACCCCCAAAGTGGAGCAGGTGCCCATCGTGGACATCATGTTCAATGACTTC
GGTGAAGCGTCACAGAAATTTGGATTTGAAGTGGGGCCGGCTTGCTTCATGGGCTTG
Protein sequence predicted based on nucleotide sequence
MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTG FCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIY NEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLI LDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHY SPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAA KETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEG EENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPY YDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLP GPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVS AQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPP GPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDD GERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPP GQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPP GPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGED GPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGR GTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFP GPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPP GPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPP GPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPV GLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEP GPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPS GAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAA GPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPT GEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLR GIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEK GDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHP GPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKL EIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTC VFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAHQ NVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVL EIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMGL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
