Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859693
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5532nts
Open reading frame: 1 to 5532
ATGGAGCCGGCCGAGCGGGCGGGAGTCGGAGAGCCCCCGGAGCCGGGCGGGCGTCCCGAG
CCGGGCCCGCGGGGCTTCGTCCCGCAGAAGGAGATCGTCTACAACAAGCTGCTGCCCTAC
GCGGAGCGGCTAGACGCCGAGTCCGACTTGCAGCTGGCCCAGATCAAATGCAACCTGGGC
CGGGCCGTGCAGCTCCAAGAGCTGTGGCCCGGGGGCCTCTTCTGGACCAGGAAACTCTCC
ACATATATTCGACTTTATGGGAGAAAATTTAGCAAAGAAGATCATGTTCTTTTTATTAAG
TTATTGTATGAGCTGGTATCAATTCCAAAACTGGAAATCAGCATGATGCAGGGATTTGCC
CGCCTTTTGATCAACTTGTTAAAGAAAAAGGAACTTCTTTCAAGAGCTGATTTGGAGTTA
CCCTGGAGACCACTTTATGACATGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTA
GGATTAAATTGGTTTCCTAATTCTGTAGAAAATATTCTCAAAACACTCGTGAAAAGCTGC
CGACCATATTTTCCAGCAGATGCCACCGCTGAGATGCTAGAAGAATGGCGACCTTTAATG
TGCCCTTTTGATGTAACCATGCAAAAGGCCATCACTTATTTTGAAATATTTCTTCCTACC
TCCCTTCCTCCAGAACTTCATCATAAAGGTTTTAAACTTTGGTTTGATGAATTAATTGGC
CTTTGGGTTTCAGTGCAAAATCTCCCACAATGGGAGGGGCAACTAGTAAATCTCTTTGCT
CGATTGGCTACAGATAATATAGGGTACATAGATTGGGATCCATATGTACCAAAGATATTT
ACAAGAATTCTGAGAAGCTTGAACCTCCCAGTGGGAAGCAGTCAAGTGTTAGTCCCAAGA
TTTTTAACAAATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCCATGATGGGT
GGACCAAGTAAGCTAGTGCAAAAACACTTAGCTGGTTTGTTTAACAGCATCACATCTTTT
TACCATCCTTCAAATAATGGGCGCTGGCTGAACAAGTTAATGAAACTACTTCAGCGGTTG
CCAAACAGTGTTGTTAGAAGATTGCATCGTGAAAGATACAAGAAGCCCTCTTGGTTAACT
CCTGTGCCTGATAGCCACAAGCTTACTGATCAAGATGTTACAGACTTTGTACAATGCATT
ATTCAGCCTGTCCTCTTGGCTATGTTTAGCAAAACCGGTAGTCTAGAAGCAGCCCAGGCT
TTGCAGAATCTTGCACTCATGAGACCTGAATTGGTAATACCCCCTGTACTTGAAAGAACA
TATCCTGCATTAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAGTTGTGTA
ATTGGAGTAGCCCGCAGTTTGGTATCAGGAGGCAGATGGTTTCCTGAAGGTCCTACACAT
ATGCTACCTCTGTTGATGAGAGCATTGCCTGGGGTGGATCCAAATGACTTTAGTAAATGC
ATGATCACATTCCAGTTCATAGCAACATTTTCTACTCTGGTGCCTTTAGTAGATTGTTCA
TCTGTACTACAAGAAAGAAATGACCTCACAGAAGTGGAACGAGAACTTTGTTCAGCCACA
GCTGAATTTGAGGATTTCGTCTTACAGTTTATGGACAGATGTTTTGGACTTATAGAAAGT
AGCACATTGGAGCAAACAAGAGAAGAGACAGAAACTGAGAAAATGACACACTTGGAGAGT
TTGGTCGAATTAGGTCTGTCTTCTACGTTTAGTACAATCCTCACCCAATGTTCCAAAGAA
ATATTTATGGTGGCCCTTCAGAAGGTTTTTAATTTTTCTACTTCACATATATTTGAAACA
AGAGTAGCAGGTCGCATGGTGGCAGACATGTGCCGCGCTGCTGTAAAGTGCTGCCCAGAA
GAATCTTTGAAGCTCTTTGTTCCCCACTGCTGCAGTGTTATAACTCAGCTTACAATGAAT
GATGATGTATTAAATGATGAAGAGCTAGACAAGGAATTACTATGGAATCTTCAACTTTTG
TCTGAGATTACTCGAGTGGATGGAAGGAAGTTGCTTCTTTATAGGGAGCAGCTTGTAAAG
ATTCTCCAAAGAACCCTACATTTAACCTGTAAGCAGGGTTACACTCTGTCTTGTAACCTT
TTGCATCATCTTCTCCGTTCTACCACACTTATCTACCCTACAGAATACTGCAGTGTGCCA
GGTGGCTTTGACAAGCCTCCTTCTGAATACTTTCCTATCAAGGACTGGGGCAAACCCGGG
GACTTGTGGAATCTGGGAATCCAGTGGCATGTTCCTTCTTCAGAAGAAGTGTCTTTTGCC
TTTTATCTTTTGGACTCCTTTCTTCAGCCTGAGCTCGTCAAACTCCAGCATTGTGGGGAT
GGAAAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGTGCACAACTGT
TTAATTGGCTCTGGAAACCTCCTACCTCCGTTGAAAGGAGAGCCAGTTACTAACTTAGTA
CCAAGTATGGTGTCCTTGGAAGAGACAAAGTTGTATACTGGACTTGAATATGATCTGTCT
CGAGAGAACCACCGAGAAGTAATTGCTACAGTTATAAGGAAACTTCTTAACCACATACTT
GATAATTCAGAAGATGATACTAAGTCATTGTTTCTTATTATAAAGATTATTGGAGACCTT
TTACAATTCCAAGGATCTCACAAGCATGAATTTGACTCCCGATGGAAAAGCTTCAACTTA
GTAAAGAAATCAATGGAAAATCGGCTCCATGGGAAAAAACAACATATCAGAGCACTGTTG
ATTGATAGAGTAATGTTACAGCATGAGCTACGGACACTAACTGTTGAGGGTTGTGAATAC
AAAAAGATACATCAAGATATGATCAGAGATCTTCTTCGTTTATCTACAAGTTCATACAGT
CAGGTGAGAAATAAGGCTCAGCAAACATTTTTTGCTGCCTTGGGAGCATATAACTTCTGT
TGCAGAGATATCATTCCCTTGGTTTTGGAGTTCTTAAGGCCTGATAGACAAGGTGTTACA
CAGCAACAATTCAAGGGTGCCTTGTACTGTCTCCTTGGAAATCACAGTGGTGTGTGCTTG
GCAAACCTTCATGATTGGGACTGTATTGTACAGACGTGGCCAGCGATTGTTTCTTCAGGG
CTTAGCCAAGCAATGTCCCTGGAAAAGCCATCAATAGTGAGATTGTTTGATGATCTTGCA
GAAAAGATTCATAGGCAGTATGAAACAATTGGCTTGGACTTCACAATTCCAAAGTCATGT
GTTGAAATAGCGGAATTACTTCAACAGTCAAAAAACCCCTCTATCAACCAGATATTGCTT
AGCCCAGAAAAAATTAAGGAAGGAATTAAACGCCAACAGGAAAAGAATGCCGATGCCCTA
AGGAACTATGAAAATTTGGTAGACACCTTGCTAGATGGTGTGGAGCAAAGAAACCTGCCC
TGGAAATTTGAACATATAGGCATTGGGCTTCTGTCTCTACTGCTGAGAGATGACCGAGTG
TTGCCTCTTCGTGCCATACGGTTTTTTGTTGAGAATCTCAACCATGATGCAATTGTAGTT
CGAAAGATGGCTATCTCAGCTGTTGCTGGTATCCTTAAACAGCTAAAAAGAACCCACAAA
AAGCTGACCATTAACCCCTGTGAAATCAGTGGATGCCCTAAACCCACCCAAATTATTGCT
GGTGATAGGCCTGATAATCATTGGTTGCATTATGACAGCAAAACTATACCAAGAACTAAA
AAAGAATGGGAGTCAAGTTGCTTTGTGGAAAAAACTCACTGGGGATACTACACCTGGCCA
AAGAATATGGTTGTTTATGCTGGTGTGGAAGAGCAGCCTAAGCTTGGCAGAAGCAGGGAG
GATATGACAGAGGCAGAACAGATTATATTTGATCATTTTTCTGATCCTAAATTTGTTGAG
CAGTTAATTACTTTTCTATCATTAGAAGACAGAAAAGGAAAAGATAAGTTTAATCCACGA
CGTTTTTGCCTCTTTAAGGGTATATTCAGGAATTTTGATGATGCCTTCCTGCCAGTTCTG
AAGCCCCATTTAGAACATTTGGTTGCAGATTCACATGAAAGCACCCAGCGATGTGTTGCA
GAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCACTGGACATTTGAAAAGGTGGAGAAG
CTTTGGGAGCTTCTGTGCCCTCTGCTTAGAACAGCACTGTCCAATATTACCGTAGAAACT
TATAATGACTGGGGAGCTTGTATAGCAACATCCTGTGAAAGCAGAGATCCCCGGAAACTT
CACTGGCTTTTTGAACTGCTGTTGGAATCACCATTGAGTGGTGAAGGAGGATCCTTTGTA
GATGCATGTCGACTTTATGTACTACAAGGTGGCCTTGCCCAGCAAGAATGGAGAGTGCCT
GAACTATTGCACAGACTACTGAAGTACTTGGAACCCAAACTCACCCAGGTTTACAAAAAT
GTCAGAGAAAGAATAGGAAGTGTGCTGACCTACATATTCATGATAGATGTATCTTTGCCA
AATACCACACCAACCATATCGCCTCATGTCCCTGAGTTTACTGCTCGAATTCTGGAGAAA
TTGAAACCTCTCATGGATGTGGATGAAGAAATTCAGAACCATGTTATGGAAGAAAATGGA
ATTGGTGAAGAAGATGAGCGAACTCAGGGCATTAAACTCTTGAAAACCATATTGAAATGG
CTGATGGCAAGTGCAGGAAGATCCTTTTCTACAGCAGTTACAGAACAACTTCAGCTTCTA
CCTTTGTTTTTCAAGATTGCCCCAGTGGAAAATGACAATAGCTACGATGAACTGAAAAGA
GATGCAAAGTTATGTTTATCATTAATGTCTCAGGGGTTGCTTTACCCTCATCAAGTGCCT
TTGGTACTTCAGGTGCTAAAACAAACAGCAAGAAGCAGTTCTTGGCATGCACGATACACA
GTACTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA
GATGCAGTTAAAGATATCAGGTGGCTGGTTATAAGTCTTTTGGAGGACGAACAACTGGAG
GTTCGAGAAATGGCTGCTACTACCTTAAGCGGTCTGCTACAGTGTAACTTTCTTACCATG
GACAGTCCTATGCAGATTCATTTTGAGCAACTTTGCAAAACAAAACTACCTAAGAAAAGA
AAGCGAGACCCTGGTTCTGTAGGAGATACCATTCCTTCTGCAGAGTTGGTCAAACGCCAT
GCTGGGGTGCTAGGACTTGGTGCATGTGTTCTTTCTAGTCCTTACGATGTTCCCACCTGG
ATGCCCCAGCTCCTCATGAATCTCAGTGCACATCTAAATGATCCTCAGCCTATTGAGATG
ACTGTAAAAAAAACCTTATCCAATTTCCGAAGGACTCACCATGACAACTGGCAGGAACAT
AAACAGCAATTCACTGATGACCAACTGCTTGTTCTCACCGATCTTCTTGTGTCACCATGC
TATTATGCATTG
Protein sequence predicted based on nucleotide sequence
MEPAERAGVGEPPEPGGRPEPGPRGFVPQKEIVYNKLLPYAERLDAESDLQLAQIKCNLG RAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFA RLLINLLKKKELLSRADLELPWRPLYDMVERILYSKTEHLGLNWFPNSVENILKTLVKSC RPYFPADATAEMLEEWRPLMCPFDVTMQKAITYFEIFLPTSLPPELHHKGFKLWFDELIG LWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPR FLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRL PNSVVRRLHRERYKKPSWLTPVPDSHKLTDQDVTDFVQCIIQPVLLAMFSKTGSLEAAQA LQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLSCVIGVARSLVSGGRWFPEGPTH MLPLLMRALPGVDPNDFSKCMITFQFIATFSTLVPLVDCSSVLQERNDLTEVERELCSAT AEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKE IFMVALQKVFNFSTSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCCSVITQLTMN DDVLNDEELDKELLWNLQLLSEITRVDGRKLLLYREQLVKILQRTLHLTCKQGYTLSCNL LHHLLRSTTLIYPTEYCSVPGGFDKPPSEYFPIKDWGKPGDLWNLGIQWHVPSSEEVSFA FYLLDSFLQPELVKLQHCGDGKLEMSRDDILQSLTIVHNCLIGSGNLLPPLKGEPVTNLV PSMVSLEETKLYTGLEYDLSRENHREVIATVIRKLLNHILDNSEDDTKSLFLIIKIIGDL LQFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEY KKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRQGVT QQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPAIVSSGLSQAMSLEKPSIVRLFDDLA EKIHRQYETIGLDFTIPKSCVEIAELLQQSKNPSINQILLSPEKIKEGIKRQQEKNADAL RNYENLVDTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVV RKMAISAVAGILKQLKRTHKKLTINPCEISGCPKPTQIIAGDRPDNHWLHYDSKTIPRTK KEWESSCFVEKTHWGYYTWPKNMVVYAGVEEQPKLGRSREDMTEAEQIIFDHFSDPKFVE QLITFLSLEDRKGKDKFNPRRFCLFKGIFRNFDDAFLPVLKPHLEHLVADSHESTQRCVA EIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNITVETYNDWGACIATSCESRDPRKL HWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKN VRERIGSVLTYIFMIDVSLPNTTPTISPHVPEFTARILEKLKPLMDVDEEIQNHVMEENG IGEEDERTQGIKLLKTILKWLMASAGRSFSTAVTEQLQLLPLFFKIAPVENDNSYDELKR DAKLCLSLMSQGLLYPHQVPLVLQVLKQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNE DAVKDIRWLVISLLEDEQLEVREMAATTLSGLLQCNFLTMDSPMQIHFEQLCKTKLPKKR KRDPGSVGDTIPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEM TVKKTLSNFRRTHHDNWQEHKQQFTDDQLLVLTDLLVSPCYYAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
