Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859747
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4185nts
Open reading frame: 1 to 4185
ATGGCAGCCCTGCTGAGATCCGCGCGTTGGTTGCTGCGTGCCGGGGCGGCCCCGCGCCTC
CCGCTCTCCCTGCGCCTCCTCCCTGGCGGCCCGGGCCGGCTGCATGCCGCCTCCTATCTG
CCCGCCGCTCGCGCCGGGCCCGTGGCCGGAGGACTACTGAGCCCAGCCAGGCTGTATGCC
ATTGCTGCCAAAGAAAAAGATATTCAAGAGGAGTCCACTTTTTCTTCTAGGAAGATTTCC
AATCAGTTTGATTGGGCTCTAATGAGACTAGATCTTTCTGTTCGAAGAACTGGCCGCATT
CCAAAGAAGCTTCTACAAAAAGTTTTTAATGATACCTGCCGCTCAGGTGGCCTAGGTGGT
AGTCATGCCTTGCTTCTACTACGTAGTTGTGGTTCTCTCTTGCCTGAACTAAAGCTTGAA
GAGAGAACAGAATTTGCTCATAGGATATGGGACACACTTCAGAAATTAGGTGCTGTGTAT
GATGTGAGTCACTATAATGCTTTACTTAAAGTCTATCTTCAAAATGAATATAAATTCTCA
CCAACTGATTTCCTGGCAAAAATGGAGGAAGCAAACATTCAACCAAATCGAGTGACATAC
CAGAGATTGATTGCTTCTTATTGTAATGTAGGAGATATTGAAGGTGCCAGCAAGATTCTT
GGATTTATGAAAACTAAGGATCTCCCAGTTACAGAGGCAGTATTCAGTGCCCTTGTGACA
GGGCATGCCAGAGCTGGTGATATGGAGAATGCAGAAAACATTCTCACAGTGATGAGAGAT
GCCGGAATTGAGCCTGGTCCAGACACATACCTCGCATTATTGAATGCATATGCTGAGAAG
GGCGACATTGACCATGTTAAGCAGACTCTGGAGAAGGTGGAGAAGTCCGAGCTTCACCTT
ATGGACCGTGATTTACTGCAAATTATTTTTAGCTTCAGTAAAGCTGGGTATCCTCAGTAT
GTCTCAGAAATTTTGGAAAAAGTTACATGTGAAAGAAGATATATTCCAGATGCAATGAAC
CTCATTTTACTTTTAGTCACTGAAAAATTGGAAGATGTAGCGTTGCAAATTTTACTAGCA
TGCCCCGTATCAAAGGAAGATGGCCCAAGTGTCTTTGGCAGTTTCTTTTTACAACACTGT
GTGACTATGAATACGCCTGTGGAGAAGCTAACAGACTACTGTAAGAAGTTAAAGGAAGTC
CAGATGCACTCCTTTCCTCTGCAGTTCACCCTCCATTGTGCTTTACTCGCCAATAAAACT
GATTTGGCAAAAGCCTTAATGAAGGCTGTGAAGGAGGAAGGTTTTCCTATCAGACCTCAC
TATTTCTGGCCATTGCTAGTTGGACGTCGGAAGGAAAAAAATGTTCAAGGTATAATTGAA
ATCCTCAAAGGAATGCAAGAATTGGGAGTACATCCTGATCAGGAAACATATACAGATTAT
GTGATTCCATGCTTTGATAGTGTAAACTCAGCACGAGCCATTTTGCAGGAAAATGGATGT
CTGTCTGATAGTGATATGTTTTCTCAAGCTGGATTGAGAAGTGAAGCAGCAAATGGGAAC
TTAGACTTTGTATTATCATTTTTGAAATCAAATACATTGCCCATCTCGCTGCAGTCTATA
AGAAGTAGCCTACTGCTAGGCTTCAGGAGGTCTATGAATATAAATCTTTGGAGCGAGATA
ACAGAATTGTTGTACAAGGATGGACGTTATTGCCAGGAGCCTCGAGGACCGACGGAAGCT
GTTGGCTATTTTCTTTATAACTTGATTGACAGCATGAGTGACTCAGAGGTACAGGCCAAG
GAGGAGCATTTGAGACAATACTTCCATCAGCTGGAGAAGATGAATGTAAAAATTCCTGAA
AATATCTACAGAGGCATTCGTAATCTCCTGGAAAGCTACCATGTTCCTGAATTGATTAAG
GATGCTCACTTGTTGGTTGAGAGTAAGAATTTAGACTTTCAAAAAACTGTGCAACTTACA
TCATCTGAATTGGAGTCCACACTTGAAACACTAAAAGCTGAAAATCAACCTATAAGAGAT
GTCCTAAAGCAACTCATATTAGTGCTTTGTTCAGAAGAGAATATGCAAAAAGCCCTTGAA
TTGAAAGCAAAATATGAATCCGACATGGTTACTGGTGGCTATGCAGCTTTAATAAATTTA
TGCTGTCGACATGATAAAGTAGAAGATGCCTTGAACTTGAAAGAAGAATTTGACCGCTTA
GATTCATCTGCTGTCCTTGACACCGGCAAGTATGTAGGCCTTGTAAGAGTATTGGCAAAG
CATGGCAAGCTCCAAGATGCTATTAACATTCTGAAGGAGATGAAAGAGAAGGATGTTCTT
ATCAAAGATACAACAGCCTTGTCCTTTTTCCACATGCTAAATGGCGCAGCTTTAAGAGGT
GAAATTGAAACAGTAAAACAGTTGCATGAAGCCATCGTGACTCTAGGGTTAGCAGAACCA
TCCACCAACATAAGTTTCCCATTGGTCACTGTACACTTGGAAAAGGGCGACCTATCTACT
GCTCTTGAGGTCGCCATTGACTGCTATGAAAAGTATAAAGTATTACCAAGGATTCATGAT
GTCTTGTGTAAACTGGTAGAGAAAGGCGAGACTGATCTAATTCAGAAAGCAATGGACTTT
GTGAGCCAAGAACAAGGTGAAATGGTGATGCTCTATGATCTCTTCTTTGCCTTCCTACAA
ACAGGAAATTACAAAGAGGCCAAGAAGATCATTGAGACTCCAGGGATTAGAGCTCGATCT
GCAAGGCTTCAGTGGTTTTGTGACAGATGTGTTGCAAATAATCAGGTTGAAACTCTGGAA
AAATTAGTGGAGCTGACACAGAAGCTATTTGAATGTGATAGAGACCAGATGTACTACAAT
CTGCTAAAACTGTATAAAATAAACGGTGACTGGCAAAGAGCTGATGCAGTCTGGAATAAA
ATCCAAGAAGAAAATGTTATTCCTCGTGAAAAGACATTAAGATTATTAGCAGAAATCCTT
AGAGAGGGTAACCAGGAAGTTCCGTTTGACGTACCTGAGTTGTGGTATGAAGATGAAAAA
CATTCCCTGAATTCTTCGTCAGCCTCAACCACAGAACCTGATTTCCAGAAAGATATATTG
ATTGCCTGCCGATTGAACCAAAAAAAAGGGGCATATGATATTTTCCTGAATGCAAAAGAG
CAAAACATTGTGTTTAATGCTGAAACCTACAGCAATCTCATTAAATTACTGATGTCAGAA
GATTATTTTACACAAGCAATGGAAGTGAAAGCATTCGCGGAGACCCACATCAAGGGCTTC
ACACTGAACGATGCTGCCAACAGCCGCCTCATCATAACGCAAGTTAGGCGGGATTATTTG
AAAGAGGCTGTGACAACACTGAAAACAGTATTGGATCAGCAGCAGACCCCTTCTAGGTTA
GCAGTGACCCGTGTCATCCAGGCATTGGCCATGAAGGGTGATGTTGAAAACATAGAAGTA
GTTCAGAAGATGTTAAATGGACTCGAAGACTCCATTGGACTTTCAAAAATGGTTTTCATC
AATAACATTGCTTTGGCTCAAATAAAGAATAATAACATAGATGCCGCAATAGAAAACATT
GAAAATATGCTTACTTCAGAGAATAAAGTCATTGAACCCCAATACTTCGGCTTGGCATAC
TTATTCAGAAAAGTAATAGAGGAGCAGTTGGAACCAGCAGTTGAAAAGATAAGCATCATG
GCGGAGAGATTGGCCAATCAGTTTGCAATTTATAAACCTGTCACTGATTTTTTCCTTCAA
CTTGTGGATGCAGGCAAGGTGGATGATGCCAGAGCTCTCCTACAGAGATGTGGTGCAATT
GCTGAACAAACCCCGATTTTGTTGTTGTTCCTCCTTAGGAATTCTAGGAAACAAGGAAAG
GCATCAACTGTGAAATCTGTGTTAGAATTGATTCCTGAATTAAATGAAAAGGAAGAAGCA
TACAATTCCCTCATGAAAAGCTATGTCTCAGAGAAAGATGTCACATCTGCTAAAGCACTG
TATGAACATTTGACTGCAAAGAATACAAAATTGGATGATCTGTTTCTAAAGCGTTACGCA
TCTTTGCTGAAGTATGCTGGAGAGCCTGTCCCTTTCATTGAACCCCCTGAAAGCTTTGAA
TTTTATGCACAGCAGCTAAGAAAATTGAGGGAAAACTCTTCTTTG
Protein sequence predicted based on nucleotide sequence
MAALLRSARWLLRAGAAPRLPLSLRLLPGGPGRLHAASYLPAARAGPVAGGLLSPARLYA IAAKEKDIQEESTFSSRKISNQFDWALMRLDLSVRRTGRIPKKLLQKVFNDTCRSGGLGG SHALLLLRSCGSLLPELKLEERTEFAHRIWDTLQKLGAVYDVSHYNALLKVYLQNEYKFS PTDFLAKMEEANIQPNRVTYQRLIASYCNVGDIEGASKILGFMKTKDLPVTEAVFSALVT GHARAGDMENAENILTVMRDAGIEPGPDTYLALLNAYAEKGDIDHVKQTLEKVEKSELHL MDRDLLQIIFSFSKAGYPQYVSEILEKVTCERRYIPDAMNLILLLVTEKLEDVALQILLA CPVSKEDGPSVFGSFFLQHCVTMNTPVEKLTDYCKKLKEVQMHSFPLQFTLHCALLANKT DLAKALMKAVKEEGFPIRPHYFWPLLVGRRKEKNVQGIIEILKGMQELGVHPDQETYTDY VIPCFDSVNSARAILQENGCLSDSDMFSQAGLRSEAANGNLDFVLSFLKSNTLPISLQSI RSSLLLGFRRSMNINLWSEITELLYKDGRYCQEPRGPTEAVGYFLYNLIDSMSDSEVQAK EEHLRQYFHQLEKMNVKIPENIYRGIRNLLESYHVPELIKDAHLLVESKNLDFQKTVQLT SSELESTLETLKAENQPIRDVLKQLILVLCSEENMQKALELKAKYESDMVTGGYAALINL CCRHDKVEDALNLKEEFDRLDSSAVLDTGKYVGLVRVLAKHGKLQDAINILKEMKEKDVL IKDTTALSFFHMLNGAALRGEIETVKQLHEAIVTLGLAEPSTNISFPLVTVHLEKGDLST ALEVAIDCYEKYKVLPRIHDVLCKLVEKGETDLIQKAMDFVSQEQGEMVMLYDLFFAFLQ TGNYKEAKKIIETPGIRARSARLQWFCDRCVANNQVETLEKLVELTQKLFECDRDQMYYN LLKLYKINGDWQRADAVWNKIQEENVIPREKTLRLLAEILREGNQEVPFDVPELWYEDEK HSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSE DYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRL AVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENI ENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQ LVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEA YNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFE FYAQQLRKLRENSSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
